A modular polycistronic expression system for overexpressing protein complexes in Escherichia coli

Protein Expression and Purification

Volumn 21, Issue 1, 2001, Pages 224-234

  Tan, Song ^a  

^a PENNSYLVANIA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EXPRESSION VECTOR; GENE EXPRESSION SYSTEM; GENE OVEREXPRESSION; GENE TAGGING; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR CLONING; PROTEIN EXPRESSION;

ESCHERICHIA COLI;

EID: 0034963119     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1363     Document Type: Article

References (21)

1. Principles of protein folding in the cellular environment
2. The human TFIID components TAF(II)135 and TAF(II)20 and the yeast SAGA components ADA1 and TAF(II)68 heterodimerize to form histone-like pairs
3. Recombinant replication protein A: Expression, complex formation, and functional characterization
4. Purification, gene cloning, and reconstitution of the heterotrimeric single-stranded DNA-binding protein from Schizosaccharomyces pombe
5. Protein self-organization in vitro and in vivo: Partitioning between physical biochemistry and cell biology
6. Coexpression of nuclear receptor partners increases their solubility and biological activities
7. Crystal structure of the nucleosome core particle at 2.8 A resolution
8. Characterization of nucleosome core particles containing histone proteins made in bacteria
9. A totally synthetic plasmid for general cloning, gene expression and mutagenesis in Escherichia coli
10. Coexpression and assembly of myosin heavy chain and myosin light chain in Escherichia coli
11. The T7 phage gene 10 leader RNA, a ribosome-binding site that dramatically enhances the expression of foreign genes in Escherichia coli
12. A novel sequence element derived from bacteriophage T7 mRNA acts as an enhancer of translation of the lacZ gene in Escherichia coli
13. Hybrid enterotoxin LTA::STa proteins and their protection from degradation by in vivo association with B-subunits of Escherichia coli heat-labile enterotoxin
14. Catalytic activity of vaccinia mRNA capping enzyme subunits coexpressed in Escherichia coli
15. Structure of the VHL-ElonginC-ElonginB complex: Implications for VHL tumor suppressor function
16. Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system
17. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
18. Use of T7 RNA polymerase to direct expression of cloned genes
19. Crystal structure of a yeast TFIIA/TBP/DNA complex
20. Structural similarity between TAFs and the heterotetrameric core of the histone octamer
21. Recombinant RNA polymerase: Inducible overexpression, purification and assembly of Escherichia coli rpo gene products