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Volumn 14, Issue 2, 2004, Pages 155-164

The functions of E(Z)/EZH2-mediated methylation of lysine 27 in histone H3

Author keywords

E(Z); EED; Embryonic ectoderm development; Enhancer of Zeste; ESC; Extra sex combs; GFP; Green fluorescent protein; Histone methyltransferase; HMTase; Maternal effect sterile; MES; PcG; Polycomb group; Polycomb repressive complex 1; PRC1; SAM; Sterile motif

Indexed keywords

COMPLEMENTARY DNA; DNA BINDING PROTEIN; EPITOPE; HISTONE H3;

EID: 1942503942     PISSN: 0959437X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.gde.2004.02.001     Document Type: Review
Times cited : (749)

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    • The role of EZH2 in breast cancer progression is investigated. A comparison of several breast samples indicates that the EZH2 level is increased in invasive breast cancer as compared with normal breast epithelia. Tissue microarray analysis indicates that the EZH2 protein level correlates with breast cancer aggressiveness. Forced expression of EZH2 in immortalized human mammary epithelial cell lines promotes anchorage-independent growth and cell invasion. Similar to the mechanism of cell invasion in metastatic prostate cancer, EZH2-mediated breast cell invasion requires an intact SET domain.
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    • EZH2 is downstream of the pRB-E2F pathway, essential for proliferation and amplified in cancer
    • Several Polycomb group proteins, including EZH2, EED and SUZ12, have been previously identified as E2F target genes by gene expression profiling and chromatin immunoprecipitation. Here, the authors show that the pRB-E2F pathway regulates the expression of EED and EZH2. RNA-mediated interference experiments show that EED and EZH2 are required for the proliferation of both transformed and nontransformed cells. Fluorescence-activated cell sorting analysis indicates that EED and EZH2 may regulate the cell cycle at the S phase. Consistently, EZH2 is highly expressed in several primary human tumors and, in some cases, this may due to an increased copy number of EZH2.
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* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.