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1
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0037423933
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Polycomb, epigenomes, and control of cell identity
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Orlando V. Polycomb, epigenomes, and control of cell identity. Cell. 112:2003;599-606.
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(2003)
Cell
, vol.112
, pp. 599-606
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Orlando, V.1
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2
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0035313867
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The Polycomb group - No longer an exclusive club?
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Brock H.W., Van Lohuizen M. The Polycomb group - no longer an exclusive club? Curr Opin Genet Dev. 11:2001;175-181.
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(2001)
Curr Opin Genet Dev
, vol.11
, pp. 175-181
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Brock, H.W.1
Van Lohuizen, M.2
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4
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0035144663
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The Polycomb-group protein Xeed and the YY1 protein interact and both induce neural tissue in Xenopus embryos
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Satijn D.P.E., Hamer C.M., Den Blaauwen J., Otte A.P. The Polycomb-group protein Xeed and the YY1 protein interact and both induce neural tissue in Xenopus embryos. Mol Cell Biol. 21:2001;1360-1369.
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(2001)
Mol Cell Biol
, vol.21
, pp. 1360-1369
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Satijn, D.P.E.1
Hamer, C.M.2
Den Blaauwen, J.3
Otte, A.P.4
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5
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0032927872
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RING1 interacts with multiple Polycomb-group proteins and displays tumorigenic activity
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Satijn D.P.E., Otte A.P. RING1 interacts with multiple Polycomb-group proteins and displays tumorigenic activity. Mol Cell Biol. 19:1999;57-68.
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(1999)
Mol Cell Biol
, vol.19
, pp. 57-68
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Satijn, D.P.E.1
Otte, A.P.2
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6
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0034531408
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Loss- and gain-of-function mutations show a polycomb group function for Ring1A in mice
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del Mar Lorente M., Marcos-Gutierrez C., Perez C., Schoorlemmer J., Ramirez A., Magin T., Vidal M. Loss- and gain-of-function mutations show a polycomb group function for Ring1A in mice. Development. 127:2000;5093-5100.
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(2000)
Development
, vol.127
, pp. 5093-5100
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Del Mar Lorente, M.1
Marcos-Gutierrez, C.2
Perez, C.3
Schoorlemmer, J.4
Ramirez, A.5
Magin, T.6
Vidal, M.7
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7
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0345269795
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Rnf2 (Ring1b) deficiency causes gastrulation arrest and cell cycle inhibition
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Voncken J.W., Roelen B.A., Roefs M., de Vries S., Verhoeven E., Marino S., Deschamps J., van Lohuizen M. Rnf2 (Ring1b) deficiency causes gastrulation arrest and cell cycle inhibition. Proc Natl Acad Sci USA. 100:2003;2468-2473.
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(2003)
Proc Natl Acad Sci USA
, vol.100
, pp. 2468-2473
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Voncken, J.W.1
Roelen, B.A.2
Roefs, M.3
De Vries, S.4
Verhoeven, E.5
Marino, S.6
Deschamps, J.7
Van Lohuizen, M.8
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8
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0034785891
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Reconstitution of a functional core polycomb repressive complex
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This report reconstitutes for the first time a functional PcG complex, PRC1. It shows that PC, PH, PSC and dRING1 are the four proteins that can act as a complex that inhibits SWI/SNF-mediated chromatin remodelling. It also shows that PSC can perform this function alone, which calls into question the minimal number of PcG proteins that is required to reconstitute a functional PcG complex.
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Francis N.J., Saurin A.J., Shao Z., Kingston R.E. Reconstitution of a functional core polycomb repressive complex. Mol Cell. 8:2001;545-556 This report reconstitutes for the first time a functional PcG complex, PRC1. It shows that PC, PH, PSC and dRING1 are the four proteins that can act as a complex that inhibits SWI/SNF-mediated chromatin remodelling. It also shows that PSC can perform this function alone, which calls into question the minimal number of PcG proteins that is required to reconstitute a functional PcG complex.
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(2001)
Mol Cell
, vol.8
, pp. 545-556
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Francis, N.J.1
Saurin, A.J.2
Shao, Z.3
Kingston, R.E.4
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9
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0035833718
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A Drosophila Polycomb complex includes Zeste and dTAFII proteins
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A large PRC1 complex is purified and it is shown that numerous general transcription factors associate with this complex. Saurian et al. underline the importance of the tissue or cell type from which PRC1 is purified. The associated proteins outnumber the PRC1 proteins when a different source (HeLa cells in [10]) for PRC1 isolation is taken.
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Saurin A.J., Shao Z., Erdjument-Bromage H., Tempst P., Kingston R.E. A Drosophila Polycomb complex includes Zeste and dTAFII proteins. Nature. 412:2001;655-660 A large PRC1 complex is purified and it is shown that numerous general transcription factors associate with this complex. Saurian et al. underline the importance of the tissue or cell type from which PRC1 is purified. The associated proteins outnumber the PRC1 proteins when a different source (HeLa cells in [10]) for PRC1 isolation is taken.
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(2001)
Nature
, vol.412
, pp. 655-660
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Saurin, A.J.1
Shao, Z.2
Erdjument-Bromage, H.3
Tempst, P.4
Kingston, R.E.5
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10
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0036340237
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The core of the polycomb repressive complex is compositionally and functionally conserved in flies and humans
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Levine S.S., Weiss A., Erdjument-Bromage H., Shao Z., Tempst P., Kingston R.E. The core of the polycomb repressive complex is compositionally and functionally conserved in flies and humans. Mol Cell Biol. 22:2002;6070-6078.
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(2002)
Mol Cell Biol
, vol.22
, pp. 6070-6078
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Levine, S.S.1
Weiss, A.2
Erdjument-Bromage, H.3
Shao, Z.4
Tempst, P.5
Kingston, R.E.6
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11
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0034794690
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Su(z)12, a novel Drosophila Polycomb group gene that is conserved in vertebrates and plants
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Birve A., Sengupta A.K., Beuchle D., Larsson J., Kennsion J.A., Rasmuson-Lestander A., Muller J. Su(z)12, a novel Drosophila Polycomb group gene that is conserved in vertebrates and plants. Development. 128:2001;3371-3379.
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(2001)
Development
, vol.128
, pp. 3371-3379
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Birve, A.1
Sengupta, A.K.2
Beuchle, D.3
Larsson, J.4
Kennsion, J.A.5
Rasmuson-Lestander, A.6
Muller, J.7
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12
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18644383738
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Histone methyltransferase activity of a Drosophila Polycomb group repressor complex
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A Drosophila PRC2 is purified that contains the ESC, E(Z) and Su(z)12 proteins. It is shown that this complex contains HMTase activity that predominantly methylates histone H3-K27. The HMTase activity resides in the E(Z) protein. The H3-K27 tag is vital to targeting PRC1 to genetic loci.
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Muller J., Hart C.M., Francis N.J., Vargas M.L., Sengupta A., Wild B., Miller E.L., O'Connor M.B., Kingston R.E., Simon J.A. Histone methyltransferase activity of a Drosophila Polycomb group repressor complex. Cell. 111:2002;197-208 A Drosophila PRC2 is purified that contains the ESC, E(Z) and Su(z)12 proteins. It is shown that this complex contains HMTase activity that predominantly methylates histone H3-K27. The HMTase activity resides in the E(Z) protein. The H3-K27 tag is vital to targeting PRC1 to genetic loci.
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(2002)
Cell
, vol.111
, pp. 197-208
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Muller, J.1
Hart, C.M.2
Francis, N.J.3
Vargas, M.L.4
Sengupta, A.5
Wild, B.6
Miller, E.L.7
O'Connor, M.B.8
Kingston, R.E.9
Simon, J.A.10
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13
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0037111831
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Histone methyltransferase activity associated with a human multiprotein complex containing the Enhancer of Zeste protein
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A human PRC2 is isolated that contains the EZH2, EED and Su(z)12 proteins and this complex contains HMTase activity. This HMTase activity preferentially methylates histone H3-K27, but to a lesser extent also H3-K9. As in Drosophila, the HMTase activity is due to the EZH2 protein that has an intact EZH2 domain. In fact two PRC2s are isolated, a large complex that co-fractionates with HDAC1 and a small PRC2 that does not contain HDAC.
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Kuzmichev A., Nishioka K., Erdjumant-Bromage H., Tempst P., Reinberg D. Histone methyltransferase activity associated with a human multiprotein complex containing the Enhancer of Zeste protein. Genes Dev. 16:2002;2893-2905 A human PRC2 is isolated that contains the EZH2, EED and Su(z)12 proteins and this complex contains HMTase activity. This HMTase activity preferentially methylates histone H3-K27, but to a lesser extent also H3-K9. As in Drosophila, the HMTase activity is due to the EZH2 protein that has an intact EZH2 domain. In fact two PRC2s are isolated, a large complex that co-fractionates with HDAC1 and a small PRC2 that does not contain HDAC.
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(2002)
Genes Dev
, vol.16
, pp. 2893-2905
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Kuzmichev, A.1
Nishioka, K.2
Erdjumant-Bromage, H.3
Tempst, P.4
Reinberg, D.5
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14
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0032087892
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The Drosophila Polycomb group gene pleiohomeotic encodes a sequence-specific DNA binding protein with homology to the multifunctional mammalian transcription factor YY1
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Brown J.L., Mucci D., Whiteley M., Dirksen M.L., Kassis J.A. The Drosophila Polycomb group gene pleiohomeotic encodes a sequence-specific DNA binding protein with homology to the multifunctional mammalian transcription factor YY1. Mol Cell. 7:1998;1057-1064.
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(1998)
Mol Cell
, vol.7
, pp. 1057-1064
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Brown, J.L.1
Mucci, D.2
Whiteley, M.3
Dirksen, M.L.4
Kassis, J.A.5
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15
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0035476530
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Establishment of Polycomb silencing requires a transient interaction between PC and ESC
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Poux S., Melfi R., Pirrotta V. Establishment of Polycomb silencing requires a transient interaction between PC and ESC. Genes Dev. 15:2001;2509-2514.
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(2001)
Genes Dev
, vol.15
, pp. 2509-2514
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Poux, S.1
Melfi, R.2
Pirrotta, V.3
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16
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0036837870
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Pleiohomeotic can link Polycomb to DNA and mediate transcriptional repression
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Mohd-Sarip A., Venturini F., Chalkley G.E., Verrijzer C.P. Pleiohomeotic can link Polycomb to DNA and mediate transcriptional repression. Mol Cell Biol. 22:2002;7473-7483.
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(2002)
Mol Cell Biol
, vol.22
, pp. 7473-7483
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Mohd-Sarip, A.1
Venturini, F.2
Chalkley, G.E.3
Verrijzer, C.P.4
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17
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0037451302
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Transcription factor YY1 functions as a PcG protein in vivo
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Atchison L., Ghias A., Wilkinson F., Bonini N., Atchison M.L. Transcription factor YY1 functions as a PcG protein in vivo. EMBO J. 22:2003;1347-1358.
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(2003)
EMBO J
, vol.22
, pp. 1347-1358
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Atchison, L.1
Ghias, A.2
Wilkinson, F.3
Bonini, N.4
Atchison, M.L.5
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18
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0344223442
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RYBP, a new repressor protein that interacts with components of the mammalian Polycomb complex, and with the transcription factor YY1
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Garcia E., Marcos-Gutierrez C., del Mar Lorente M., Moreno J.C., Vidal M. RYBP, a new repressor protein that interacts with components of the mammalian Polycomb complex, and with the transcription factor YY1. EMBO J. 18:1999;3404-3418.
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(1999)
EMBO J
, vol.18
, pp. 3404-3418
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Garcia, E.1
Marcos-Gutierrez, C.2
Del Mar Lorente, M.3
Moreno, J.C.4
Vidal, M.5
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19
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0035833726
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General transcription factors bind promotors repressed by Polycomb group proteins
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Chromatin immunoprecipitation experiments are here used to study the presence of PcG proteins and general transcription factors on promoter regions of Drosophila PcG target genes. PcG proteins are found to be present on promoters of repressed genes, but so are several general transcription factors. The data suggest that PcG complexes mediate silencing by interfering with the general transcription machinery.
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Breiling A., Turner B.M., Bianchi M.E., Orlando V. General transcription factors bind promotors repressed by Polycomb group proteins. Nature. 412:2001;651-655 Chromatin immunoprecipitation experiments are here used to study the presence of PcG proteins and general transcription factors on promoter regions of Drosophila PcG target genes. PcG proteins are found to be present on promoters of repressed genes, but so are several general transcription factors. The data suggest that PcG complexes mediate silencing by interfering with the general transcription machinery.
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(2001)
Nature
, vol.412
, pp. 651-655
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Breiling, A.1
Turner, B.M.2
Bianchi, M.E.3
Orlando, V.4
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20
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0035852632
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The E2F6 transcription factor is a component of the mammalian Bmi1-containing Polycomb complex
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Trimarchi J.M., Fairchild B., Wen J., Lees J.A. The E2F6 transcription factor is a component of the mammalian Bmi1-containing Polycomb complex. Proc Natl Acad Sci USA. 98:2001;1519-1524.
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(2001)
Proc Natl Acad Sci USA
, vol.98
, pp. 1519-1524
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Trimarchi, J.M.1
Fairchild, B.2
Wen, J.3
Lees, J.A.4
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22
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0034785586
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Linking the Rb and Polycomb pathways
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Rb is involved in gene repression in an HDAC-dependent and a HDAC-independent pathway. The paper shows that the HDAC-independent pathway uses PRC1 to repress gene activity. Specifically, the human PcG protein HPC2 and the co-repressor CtBP are required. The data demonstrate functional links between a 'core' PRC1 and more loosely associated factors such as CtBP and the general transcription factor E2F.
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Dahiya A., Wong S., Gonzalo S., Gavin M., Dean D.C. Linking the Rb and Polycomb pathways. Mol Cell. 8:2001;557-569 Rb is involved in gene repression in an HDAC-dependent and a HDAC-independent pathway. The paper shows that the HDAC-independent pathway uses PRC1 to repress gene activity. Specifically, the human PcG protein HPC2 and the co-repressor CtBP are required. The data demonstrate functional links between a 'core' PRC1 and more loosely associated factors such as CtBP and the general transcription factor E2F.
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(2001)
Mol Cell
, vol.8
, pp. 557-569
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Dahiya, A.1
Wong, S.2
Gonzalo, S.3
Gavin, M.4
Dean, D.C.5
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23
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0032931768
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C-Terminal binding protein is a transcriptional repressor that interacts with a specific class of vertebrate Polycomb proteins
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Sewalt R.G.A.B., Gunster M.J., van der Vlag J., Satijn D.P.E., Otte A.P. C-Terminal binding protein is a transcriptional repressor that interacts with a specific class of vertebrate Polycomb proteins. Mol Cell Biol. 19:1999;777-787.
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(1999)
Mol Cell Biol
, vol.19
, pp. 777-787
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Sewalt, R.G.A.B.1
Gunster, M.J.2
Van Der Vlag, J.3
Satijn, D.P.E.4
Otte, A.P.5
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24
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0037418829
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The Polycomb protein Pc2 is a SUMO E3
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The HPC2 PcG protein is shown to be involved in sumoylation of the CtBP co-repressor. The addition of a SUMO group to CtBP presumably influences the activity of CtBP. Another consequence of CtBP sumoylation is that CtBP is targeted to large PcG domains in the cell nucleus.
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Kagey M.H., Melhuish T.A., Wotton D. The Polycomb protein Pc2 is a SUMO E3. Cell. 113:2003;127-137 The HPC2 PcG protein is shown to be involved in sumoylation of the CtBP co-repressor. The addition of a SUMO group to CtBP presumably influences the activity of CtBP. Another consequence of CtBP sumoylation is that CtBP is targeted to large PcG domains in the cell nucleus.
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(2003)
Cell
, vol.113
, pp. 127-137
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Kagey, M.H.1
Melhuish, T.A.2
Wotton, D.3
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25
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0032751323
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Transcriptional repression mediated by the human Polycomb-group protein EED involves histone deacetylation
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Van der Vlag J., Otte A.P. Transcriptional repression mediated by the human Polycomb-group protein EED involves histone deacetylation. Nat Genet. 23:1999;474-478.
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(1999)
Nat Genet
, vol.23
, pp. 474-478
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Van Der Vlag, J.1
Otte, A.P.2
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26
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0035141264
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The Drosophila Polycomb Group proteins ESC and E(Z) are present in a complex containing the histone-binding protein p55 and the histone deacetylase RPD3
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The Drosophila HDAC RPD3 is found to be in a complex with ESC and E(Z), the PRC2. Functional RPD3 is needed for a PRE to mediate repression of a target gene properly. Furthermore, RPD3 binds directly to the ESC protein as well as to the PRE in vivo.
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Tie F., Furuyama T., Prasad-Sinha J., Jane E., Harte P.J. The Drosophila Polycomb Group proteins ESC and E(Z) are present in a complex containing the histone-binding protein p55 and the histone deacetylase RPD3. Development. 128:2001;275-286 The Drosophila HDAC RPD3 is found to be in a complex with ESC and E(Z), the PRC2. Functional RPD3 is needed for a PRE to mediate repression of a target gene properly. Furthermore, RPD3 binds directly to the ESC protein as well as to the PRE in vivo.
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(2001)
Development
, vol.128
, pp. 275-286
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Tie, F.1
Furuyama, T.2
Prasad-Sinha, J.3
Jane, E.4
Harte, P.J.5
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27
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0035859854
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Essential role of Drosophila Hdac1 in homeotic gene silencing
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Chang Y.-L., Peng Y.-H., Pan I.-C., Sun D.-S., King B., Huang D.-H. Essential role of Drosophila Hdac1 in homeotic gene silencing. Proc Natl Acad Sci USA. 98:2001;9730-9735.
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(2001)
Proc Natl Acad Sci USA
, vol.98
, pp. 9730-9735
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Chang, Y.-L.1
Peng, Y.-H.2
Pan, I.-C.3
Sun, D.-S.4
King, B.5
Huang, D.-H.6
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28
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0037131523
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Drosophila enhancer of Zeste/ESC complexes have a histone H3 methyltransferase activity that marks chromosomal Polycomb sites
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A Drosophila PRC2 containing ESC, RPD3, E(Z) and Su(z)12 is purified and shown to contain HMTase activity that is due to the E(Z) protein that has an intact SET domain. It is actually shown that the PC protein binds in vitro to methylated H3-K27.
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Czermin B., Melfi R., McCabe D., Seitz V., Imhof A., Pirrotta V. Drosophila enhancer of Zeste/ESC complexes have a histone H3 methyltransferase activity that marks chromosomal Polycomb sites. Cell. 111:2002;185-196 A Drosophila PRC2 containing ESC, RPD3, E(Z) and Su(z)12 is purified and shown to contain HMTase activity that is due to the E(Z) protein that has an intact SET domain. It is actually shown that the PC protein binds in vitro to methylated H3-K27.
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(2002)
Cell
, vol.111
, pp. 185-196
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Czermin, B.1
Melfi, R.2
McCabe, D.3
Seitz, V.4
Imhof, A.5
Pirrotta, V.6
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29
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0037404421
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A 1-Megadalton ESC/E(Z) complex from Drosophila that contains Polycomblike and RPD3
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Two PRC2s are isolated from Drosophila embryos. One large, 1Mda complex, contains the ESC, Su(z)12 and E(Z) proteins, but also the PcG protein Polycomblike and the HDAC RPD3. A second PRC2 of ∼600kDa does not contain the Polycomblike or the RPD3 proteins. On polytene chromosomes, binding sites for E(Z) overlap almost completely with the binding sites for Polycomblike, as well as with a subset of RPD3 binding sites. They discuss that other groups may purify the 600kDa PRC2, which could explain the conflicting results on the presence of different associated gene regulatory factors in PRC preparations.
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Tie F., Prasad-Sinha J., Birve A., Rasmuson-Lestander A., Harte P.J. A 1-Megadalton ESC/E(Z) complex from Drosophila that contains Polycomblike and RPD3. Mol Cell Biol. 23:2003;3352-3362 Two PRC2s are isolated from Drosophila embryos. One large, 1Mda complex, contains the ESC, Su(z)12 and E(Z) proteins, but also the PcG protein Polycomblike and the HDAC RPD3. A second PRC2 of ∼600kDa does not contain the Polycomblike or the RPD3 proteins. On polytene chromosomes, binding sites for E(Z) overlap almost completely with the binding sites for Polycomblike, as well as with a subset of RPD3 binding sites. They discuss that other groups may purify the 600kDa PRC2, which could explain the conflicting results on the presence of different associated gene regulatory factors in PRC preparations.
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(2003)
Mol Cell Biol
, vol.23
, pp. 3352-3362
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Tie, F.1
Prasad-Sinha, J.2
Birve, A.3
Rasmuson-Lestander, A.4
Harte, P.J.5
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30
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0036375953
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A panel of monoclonal antibodies against human polycomb group proteins
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Hamer C.M., Sewalt R.G.A.B., Den Blaauwen J.L., Hendrix M., Satijn D.P.E., Otte A.P. A panel of monoclonal antibodies against human polycomb group proteins. Hybridoma Hybridomics. 21:2002;245-252.
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(2002)
Hybridoma Hybridomics
, vol.21
, pp. 245-252
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Hamer, C.M.1
Sewalt, R.G.A.B.2
Den Blaauwen, J.L.3
Hendrix, M.4
Satijn, D.P.E.5
Otte, A.P.6
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31
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0035036571
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Differential expression of human Polycomb group proteins in various tissues and cell types
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Immunohistochemistry on different human tissues with a panel of antibodies against human PcG proteins is used to study the dynamic distributions of PcG complexes. In virtually all different cell types the compositions of even the 'core' PRCs differ completely. Particularly during the development of fetal kidney cells the composition of PRC1 changes dramatically when cells proceed from one developmental stage to the next. The data argue against an oversimplified view of PcG proteins being distributed over only two 'core' PRCs.
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Gunster M.J., Raaphorst F.M., Hamer C.M., Den Blaauwen J.L., Fieret E., Meijer C.J.L.M., Otte A.P. Differential expression of human Polycomb group proteins in various tissues and cell types. J Cell Biochem. 36:2001;129-143 Immunohistochemistry on different human tissues with a panel of antibodies against human PcG proteins is used to study the dynamic distributions of PcG complexes. In virtually all different cell types the compositions of even the 'core' PRCs differ completely. Particularly during the development of fetal kidney cells the composition of PRC1 changes dramatically when cells proceed from one developmental stage to the next. The data argue against an oversimplified view of PcG proteins being distributed over only two 'core' PRCs.
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(2001)
J Cell Biochem
, vol.36
, pp. 129-143
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Gunster, M.J.1
Raaphorst, F.M.2
Hamer, C.M.3
Den Blaauwen, J.L.4
Fieret, E.5
Meijer, C.J.L.M.6
Otte, A.P.7
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32
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0036830642
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Role of histone H3 lysine 27 methylation in Polycomb-group silencing
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Cao R., Wang L., Wang H., Xia L., Erdjument-Bromage H., Tempst P., Jones R.S., Zhang Y. Role of histone H3 lysine 27 methylation in Polycomb-group silencing. Science. 298:2002;1039-1043.
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(2002)
Science
, vol.298
, pp. 1039-1043
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Cao, R.1
Wang, L.2
Wang, H.3
Xia, L.4
Erdjument-Bromage, H.5
Tempst, P.6
Jones, R.S.7
Zhang, Y.8
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33
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0034632829
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Regulation of chromatin structure by site-specific histone H3 methyltransferases
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Rea S.F., Eisenhaber D., O'Carroll D., Strahl B.D., Sun Z.W., Schmid M., Opravil S., Mechtler K., Ponting C.P., Allis C.D., Jenuwein T. Regulation of chromatin structure by site-specific histone H3 methyltransferases. Nature. 406:2000;593-599.
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(2000)
Nature
, vol.406
, pp. 593-599
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Rea, S.F.1
Eisenhaber, D.2
O'Carroll, D.3
Strahl, B.D.4
Sun, Z.W.5
Schmid, M.6
Opravil, S.7
Mechtler, K.8
Ponting, C.P.9
Allis, C.D.10
Jenuwein, T.11
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34
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0035282573
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Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins
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Lachner M., O'Carroll D., Rea S., Mechtler K., Jenuwein T. Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins. Nature. 410:2001;116-120.
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(2001)
Nature
, vol.410
, pp. 116-120
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Lachner, M.1
O'Carroll, D.2
Rea, S.3
Mechtler, K.4
Jenuwein, T.5
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35
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0036310641
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Selective interactions between vertebrate Polycomb homologs and the SUV39H1 HMTase suggest histone H3-K9 methylation to contribute to chromosomal targeting of Polycomb-group proteins
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