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ChIP assays were here used for the systematic analysis of the promoter and open reading frame occupancy by transcription initiation and elongation factors in vivo. Two major novel observations were made. First, the distribution of the Paf1/Cdc73 complex was found to be similar to that of the known transcript elongation factors, TFIIS and Spt5. This observation suggested a role for the Paf1/Cdc73 complex in transcript elongation. Second, Elongator was not detected in the coding regions of the genes tested and, as was revealed by the immunofluorescence studies, it was mostly localized to the cytoplasm. Thus the role for the Elongator in the PolII transcription apparatus became more obscure.
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