Genetic interactions of Spt4-Spt5 and TFIIS with the RNA polymerase II CTD and CTD modifying enzymes in Saccharomyces cerevisiae

Genetics

Volumn 159, Issue 2, 2001, Pages 487-497

  Lindstrom, Derek L ^a   Hartzog, Grant A ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ELONGATION FACTOR; ELONGATION FACTOR SPT4; ELONGATION FACTOR SPT5; ELONGATION FACTOR SPT6; ELONGATION FACTOR TFIIS; HEPTAPEPTIDE; PROTEIN KINASE; PROTEIN KINASE BUR1; PROTEIN KINASE CTK1; PROTEIN KINASE KIN28; RNA POLYMERASE II; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; ENZYME ACTIVITY; ENZYME BINDING; GENE MUTATION; GENETIC TRANSCRIPTION; IMMUNOPRECIPITATION; MOLECULAR INTERACTION; NONHUMAN; NUCLEOTIDE REPEAT; PHENOTYPE; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS;

BLOTTING, WESTERN; CHROMOSOMAL PROTEINS, NON-HISTONE; FUNGAL PROTEINS; GENES, FUNGAL; NUCLEAR PROTEINS; PRECIPITIN TESTS; PROTEIN KINASES; RNA POLYMERASE II; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SERINE; TRANSCRIPTION FACTORS; TRANSCRIPTION FACTORS, GENERAL; TRANSCRIPTIONAL ELONGATION FACTORS;

EID: 0034764781     PISSN: 00166731     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (61)

1. Distinct activated and non-activated RNA polymerase II complexes in yeast
2. High-resolution localization of Drosophila Spt5 and Spt6 at heat shock genes in vivo: Roles in promoter proximal pausing and transcription elongation
3. Regulated phosphorylation of the RNA polymerase II C-terminal domain (CTD)
4. Isolation, sequencing, and mapping of the human homologue of the yeast transcription factor, SPT5
5. Isolation and characterization of the human and mouse homologues (SUPT4H and Supt4h) of the yeast SPT4 gene
6. A protein phosphatase functions to recycle RNA polymerase II
7. Synthetic lethal interactions suggest a role for the Saccharomyces cerevisiae Rtf1 protein in transcription elongation
8. Reversible phosphorylation of the C-terminal domain of RNA polymerase II
9. The yeast protein complex containing cdc68 and pob3 mediates core-promoter repression through the cdc68 N-terminal domain
10. A novel human SRB/MED-containing cofactor complex. SMCC, involved in transcription regulation
11. Synthetic enhancement in gene interaction: A genetic tool come of age
12. A regulator of transcriptional elongation controls vertebrate neuronal development
13. Identification and analysis of a functional human homolog of the SPT4 gene of Saccharomyces cerevisiae
14. Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae
15. Temporal regulation of RNA polymerase II by Srb10 and Kin28 cyclin-dependent kinases
16. Domains in the SPT5 protein that modulate its transcriptional regulatory properties
17. Spt5 and spt6 are associated with active transcription and have characteristics of general elongation factors in D. melanogaster
18. P-TEFb phosphorylates hSPT5 and RNA polymerase II CTD independently of CAK
19. Purification of RNA polymerase IIO from calf thymus
20. An unusual eukaryotic protein phosphatase required for transcription by RNA polymerase II and CTD dephosphorylation in S. cerevisiae
21. Different phosphorylated forms of RNA polymerase II and associated mRNA processing factors during transcription
22. Functional relationships of Srb10-Srb11 kinase, carboxy-terminal domain kinase CTDK-1, and transcriptional corepressor Ssn6-Tupl
23. CTD kinase large subunit is encoded by CTK1, a gene required for normal growth of Saccharomyces cerevisiae
24. Modulation of RNA polymerase II elongation efficiency by C-terminal heptapeptide repeat domain kinase I
25. Transcription of eukaryotic protein-coding genes
26. Requirement of RSF and FACT for transcription of chromatin templates in vitro
27. Mutations in SPT16/CDC68 suppress cis- and transacting mutations that affect promoter function in Saccharomyces cerevisiae
28. P-TEFb kinase is required for HIV Tat transcriptional activation in vivo and in vitro
29. Purification of P-TEFb, a transcription factor required for the transition into productive elongation
30. Control of RNA polymerase II elongation potential by a novel carboxyl-terminal domain kinase
31. A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix
32. Phosphorylation of the RNA polymerase II Carboxy-terminal domain by the Burl cyclin-dependent kinase
33. Intragenic and extragenic suppressors of mutations in the heptapeptide repeat domain of Saccharomyces cerevisiae RNA polymerase II
34. Functional redundancy and structural polymorphism in the large subunit of RNA polymerase II
35. FACT, a factor that facilitates transcript elongation through nucleosomes
36. The chromatin-specific transcription elongation factor FACT comprises human SPT16 and SSRP1 proteins
37. Growth-related changes in phosphorylation of yeast RNA polymerase II
38. Yeast carboxyl-terminal domain kinase I positively and negatively regulates RNA polymerase II carboxyl-terminal domain phosphorylation
39. DSIF and NELF interact with RNA Polymerase II elongation complex and HIV-1 Tat stimulates P-TEFb-mediated phosphorylation of RNA polymerase II and DSIF during transcription elongation
40. Mutations that suppress the deletion of an upstream activating sequence in yeast: Involvement of a protein kinase and histone H3 in repressing transcription in vivo
41. P-TEFb, a cyclin-dependent kinase controlling elongation by RNA polymerase II
42. Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II
43. CDC68, a yeast gene that affects regulation of cell proliferation and transcription, encodes a protein with a highly acidic carboxyl terminus
44. The yeast carboxyl-terminal repeat domain kinase CTDK-I is a divergent cyclin-cyclin-dependent kinase complex
45. NAT, a human complex containing Srb polypeptides that functions as a negative regulator of activated transcription
46. SPT4, SPT5 and SPT6 interactions: Effects on transcription and viability in Saccharomyces cerevisiae
47. SPT5, an essential gene important for normal transcription in Saccharomyces cerevisiae, encodes an acidic nuclear protein carboxy-terminal repeat
48. Inhibition of in vivo and in vitro transcription by monoclonal antibodies prepared against wheat germ RNA polymerase II that react with the heptapeptide repeat of eukaryotic RNA polymerase II
49. Basic mechanisms of transcript elongation and its regulation
50. DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs
51. Evidence that P-TEFb alleviates the negative effect of DSIF on RNA polymerase II-dependent transcription in vitro
52. FACT relieves DSIF/NELF-mediated inhibition of transcriptional elongation and reveals functional differences between P-TEFb and TFIIH
53. Cytostellin: A novel, highly conserved protein that undergoes continuous redistribution during the cell
54. Construction and analysis of yeast RNA polymerase II CTD deletion and substitution mutations
55. Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae
56. Construction of a set of convenient S. cerevisiae strains that are isogenic to S288C
57. The Saccharomyces cerevisiae DNA polymerase alpha catalytic subunit interacts with Cdc68/Spt16 and with Pob3, a protein similar to an HMG1-like protein
58. Role of the human homolog of the yeast transcription factor SPT5 in HIV-1 Tat-activation
59. NELF, a multisubunit complex containing RD, cooperates with DSIF to repress RNA polymerase II elongation
60. Transcription elongation factor P-TEFb is required for HIV-1 tat transactivation in vitro