High-resolution localization of Drosophila Spt5 and Spt6 at heat shock genes in vivo: Roles in promoter proximal pausing and transcription elongation

Genes and Development

Volumn 14, Issue 20, 2000, Pages 2635-2649

  Andrulis, Erik D ^a   Guzmán, Ernesto ^a   Döring, Pascal ^a,b   Werner, Janis ^a   Lis, John T ^b  

^a Department of Population Medicine and Diagnostic Sciences   (United States)

^b GOETHE UNIVERSITY   (Germany)

Author keywords

Elongation; Heat shock genes; P TEFb; Pol II pausing; Spt5; Spt6

Indexed keywords

CYCLINE; ELONGATION FACTOR; HEAT SHOCK PROTEIN; RNA POLYMERASE II;

ARTICLE; CROSS LINKING; DROSOPHILA; GENE FUNCTION; GENE LOCATION; HEAT SHOCK; IMMUNOFLUORESCENCE; IMMUNOHISTOCHEMISTRY; IMMUNOPRECIPITATION; NONHUMAN; POLYMERASE CHAIN REACTION; POLYTENE CHROMOSOME; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DNA INTERACTION; TRANSCRIPTION REGULATION; TRANSGENE;

ANIMALS; CHROMOSOMAL PROTEINS, NON-HISTONE; CHROMOSOMES; CROSS-LINKING REAGENTS; CYCLINS; DNA-BINDING PROTEINS; DROSOPHILA; DROSOPHILA PROTEINS; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; FUNGAL PROTEINS; GENE EXPRESSION REGULATION; HEAT-SHOCK PROTEINS; HEAT-SHOCK RESPONSE; HSP70 HEAT-SHOCK PROTEINS; INSECT PROTEINS; NUCLEAR PROTEINS; PROMOTER REGIONS (GENETICS); RNA POLYMERASE II; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC; TRANSCRIPTIONAL ELONGATION FACTORS;

EID: 0034667949     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: 10.1101/gad.844200     Document Type: Article

References (36)

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15. The nonphosphorylated form of RNA polymerase II preferentially associates with the preinitiation complex
16. Purification of P-TEFb, a transcription factor required for the transition into productive elongation
17. Rapid changes in Drosophila transcription after an instantaneous heat shock
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19. In vivo transcriptional pausing and cap formation on three Drosophila heat shock genes
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