Spt5 and Spt6 are associated with active transcription and have characteristics of general elongation factors in D. melanogaster

Genes and Development

Volumn 14, Issue 20, 2000, Pages 2623-2634

  Kaplan, C D ^a   Morris, J R ^a   Wu, C T ^a   Winston, F ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

CTD; Elongation; P TEFb; Polytene; RNA polymerase; Spt

Indexed keywords

ELONGATION FACTOR; RNA POLYMERASE II; TRANSACTIVATOR PROTEIN;

ARTICLE; DEVELOPMENT; DROSOPHILA MELANOGASTER; HEAT SHOCK; NONHUMAN; NUCLEOTIDE SEQUENCE; PHOSPHORYLATION; POLYTENE CHROMOSOME; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; SACCHAROMYCES CEREVISIAE; SALIVARY GLAND; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; TRANSACTIVATION; TRANSCRIPTION REGULATION;

ANIMAL; CHROMOSOMES; CYCLINS; DROSOPHILA MELANOGASTER; EMBRYO, NONMAMMALIAN; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GLUE PROTEINS, DROSOPHILA; HEAT-SHOCK RESPONSE; INSECT PROTEINS; MOLECULAR SEQUENCE DATA; NUCLEAR PROTEINS; PEPTIDE ELONGATION FACTORS; RNA POLYMERASE II; SEQUENCE ANALYSIS; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.; TRANSCRIPTION, GENETIC;

DROSOPHILA MELANOGASTER;

EID: 0034667805     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: 10.1101/gad.831900     Document Type: Article

References (83)

1. Basic local alignment search tool
2. High resolution of Drosophila Spt5 and Spt6 at heat shock genes in vivo: Roles in promoter proximal pausing and transcription elongation
3. Conserved domains in DNA repair proteins and evolution of repair systems
4. FCP1, the RAP74-interacting subunit of a human protein phosphatase that dephosphorylates the carboxy-terminal domain of RNA polymerase IIO
5. Patterns of puffing activity in the salivary gland chromosomes of Drosophila. I. Autosomal puffing patterns in a laboratory stock of Drosophila melanogaster
6. Patterns of puffing activity in the salivary gland chromosomes of Drosophila. VI. Induction by ecdysone in salivary glands of D. melanogaster cultured in vitro
7. Temporal control of puffing activity in polytene chromosomes
8. Coupling RNA polymerase II transcription with pre-mRNA processing
9. Evidence that Spt6p controls chromatin structure by a direct interaction with histones
10. Cytostellin distributes to nuclear regions enriched with splicing factors
11. Combinatorial effects of NusA and NusG on transcription elongation and Rho-dependent termination in Escherichia coli
12. The solution structure of the S1 RNA binding domain: A member of an ancient nucleic acid-binding fold
13. The H3/H4 tetramer blocks transcript elongation by RNA polymerase II in vitro
14. A fluorescent quantitative PCR approach to map gene deletions in the Drosophila genome
15. A protein phosphatase functions to recycle RNA polymerase II
16. Mutations in the SPT4, SPT5, and SPT6 genes alter transcription of a subset of histone genes in Saccharomyces cerevisiae
17. Reversible phosphorylation of the C-terminal domain of RNA polymerase II
18. The helix-hairpin-helix DNA-binding motif: A structural basis for nonsequence-specific recognition of DNA
19. A new gene locus of Bordetella pertussis defines a novel family of prokaryotic transcriptional accessory proteins
20. HIV-1 Tat: Coping with negative elongation factors
21. Structure and mechanism in transcriptional antitermination by the bacteriophage lambda N protein
22. Nucleosomes and transcription: Recent lessons from genetics
23. Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae
24. Lentivirus Tat proteins specifically associate with a cellular protein kinase,TAK, that hyperphosphorylates the carboxy-terminal domain of the large subunit of RNA polymerase II: Candidate for a Tat cofactor
25. The transcriptional switch between the Drosophila genes Pig-1 and Sgs-4 depends on a SEBP1 binding site within a shared enhancer region
26. Puffs and PCR: The in vivo dynamics of early gene expression during ecdysone responses in Drosophila
27. Domains in the SPT5 protein that modulate its transcriptional regulatory properties
28. Taking a new TAK on tat transactivation
29. Stability of Drosophila RNA polymerase II elongation complexes in vitro
30. Splicing factors associate with hyperphosphorylated RNA polymerase II in the absence of pre-mRNA
31. Tat-SF1 protein associates with RAP30 and human SPT5 proteins
32. The helix-loop-helix proteins dAP-4 and daughterless bind both in vitro and in vivo to SEBP3 sites required for transcriptional activation of the Drosophila gene Sgs-4
33. Promoter is an important determinant of developmentally regulated puffing at the Sgs-4 locus of Drosophila melanogaster
34. Drosophila Sgs genes: Stage and tissue specificity of hormone responsiveness
35. NusG, a new Escherichia coli elongation factor involved in transcriptional antitermination by the N protein of phage lambda
36. The NusA and NusG proteins of Escherichia coli increase the in vitro readthrough frequency of a transcriptional attenuator preceding the gene for the beta subunit of RNA polymerase
37. Promoter-associated pausing in promoter architecture and postinitiation transcriptional regulation
38. Cloning and characterization of nine heat-shock-induced mRNAs of Drosophila melanogaster
39. P-TEFb kinase recruitment and function at heat shock loci
40. Use of insoluble fusion proteins to purify antibodies
41. The transition from initiation to elongation by RNA polymerase II
42. The Drosophila fork head factor directly controls larval salivary gland-specific expression of the glue protein gene Sgs3
43. Mutations in SPT16/CDC68 suppress cis- and trans-acting mutations that affect promoter function in Saccharomyces cerevisiae
44. Control of formation of two distinct classes of RNA polymerase II elongation complexes
45. Purification of P-TEFb, a transcription factor required for the transition into productive elongation
46. A transcriptional switch between the Pig-1 and Sgs-4 genes of Drosophila melanogaster
47. The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain
48. Phosphorylation of RNA polymerase II C-terminal domain and transcriptional elongation
49. The chromatin-specific transcription elongation factor FACT comprises human SPT16 and SSRP1 proteins
50. Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation
51. A novel RNA polymerase II-containing complex potentiates Tat-enhanced HIV-1 transcription
52. Imaging neuronal subsets and other cell types in whole-mount Drosophila embryos and larvae using antibody probes
53. Growth-related changes in phosphorylation of yeast RNA polymerase II
54. P-TEFb, a cyclin-dependent kinase controlling elongation by RNA polymerase II
55. The RNA polymerase II general elongation factors
56. Mechanism and regulation of transcriptional elongation by RNA polymerase II
57. Antitermination by bacteriophage lambda Q protein
58. Factors regulating the transcriptional elongation activity of RNA polymerase II
59. The RNA polymerase II general elongation complex
60. HSF recruitment and loss at most Drosophila heat shock loci is coordinated and depends on proximal promoter sequences
61. Human Supt5h protein, a putative modulator of chromatin structure, is reversibly phosphorylated in mitosis
62. Requirement for E. coli NusG protein in factor-dependent transcription termination
63. SPT6, an essential gene that affects transcription in Saccharomyces cerevisiae, encodes a nuclear protein with an extremely acidic amino terminus
64. SPT5, an essential gene important for normal transcription in Saccharomyces cerevisiae, encodes an acidic nuclear protein with a carboxy-terminal repeat
65. Pig heart calpastatin: Identification of repetitive domain structures and anomalous behavior in polyacrylamide gel electrophoresis
66. A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast
67. Puffs and gene regulation-molecular insights into the Drosophila ecdysone regulatory hierarchy
68. SMRTER, a Drosophila nuclear receptor coregulator, reveals that EcR-mediated repression is critical for development
69. Cytogenetic and genetic mapping of a salivary gland secretion protein in Drosophila melanogaster
70. DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs
71. Evidence that P-TEFb alleviates the negative effect of DSIF on RNA polymerase II-dependent transcription in vitro
72. FACT relieves DSIF/NELF-mediated inhibition of transcriptional elongation and reveals functional differences between P-TEFb and TFIIH
73. Cytostellin: A novel, highly conserved protein that undergoes continuous redistribution during the cell cycle
74. Locus-specific variation in phosphorylation state of RNA polymerase II in vivo: Correlations with gene activity and transcript processing
75. Transcription elongation factor hSPT5 stimulates mRNA capping
76. Construction and analysis of yeast RNA polymerase II CTD deletion and substitution mutations
77. The SAGA of Spt proteins and transcriptional analysis in yeast: Past, present, and future
78. Role of the human homolog of the yeast transcription factor SPT5 in HIV-1 Tat-activation
79. Interplay between positive and negative elongation factors: Drawing a new view of DRB
80. NELF, a multisubunit complex containing RD, cooperates with DSIF to repress RNA polymerase II elongation
81. Structure and function of the human transcription elongation factor DSIF
82. Suppression analysis reveals a functional difference between the serines in positions two and five in the consensus sequence of the C-terminal domain of yeast RNA polymerase II
83. The C-terminal domain of the largest subunit of RNA polymerase II interacts with a novel set of serine/arginine-rich proteins