-
1
-
-
0026599021
-
-
G. Felsenfeld, Nature 355, 219 (1992); S. M. Paranjape, R. T. Kamakaka, J. T. Kadonaga, Annu. Rev. Bachern. 63, 265 (1994).
-
(1992)
Nature
, vol.355
, pp. 219
-
-
Felsenfeld, G.1
-
2
-
-
0028239906
-
-
G. Felsenfeld, Nature 355, 219 (1992); S. M. Paranjape, R. T. Kamakaka, J. T. Kadonaga, Annu. Rev. Bachern. 63, 265 (1994).
-
(1994)
Annu. Rev. Bachern.
, vol.63
, pp. 265
-
-
Paranjape, S.M.1
Kamakaka, R.T.2
Kadonaga, J.T.3
-
5
-
-
0028314013
-
-
B. R. Cairns, Y. J. Kim, M. H. Sayre, B. C. Laurent, R. D. Komberg, Proc. Natl. Acad. Sci. U.S.A. 91, 1950 (1994); C. L. Peterson, A. Dingwall, M. P. Scott, ibid., p. 2905; J. Côté et al., Science 265, 53 (1994).
-
(1994)
Proc. Natl. Acad. Sci. U.S.A.
, vol.91
, pp. 1950
-
-
Cairns, B.R.1
Kim, Y.J.2
Sayre, M.H.3
Laurent, B.C.4
Komberg, R.D.5
-
6
-
-
0028325734
-
-
B. R. Cairns, Y. J. Kim, M. H. Sayre, B. C. Laurent, R. D. Komberg, Proc. Natl. Acad. Sci. U.S.A. 91, 1950 (1994); C. L. Peterson, A. Dingwall, M. P. Scott, ibid., p. 2905; J. Côté et al., Science 265, 53 (1994).
-
Proc. Natl. Acad. Sci. U.S.A.
, pp. 2905
-
-
Peterson, C.L.1
Dingwall, A.2
Scott, M.P.3
-
7
-
-
0028467446
-
-
B. R. Cairns, Y. J. Kim, M. H. Sayre, B. C. Laurent, R. D. Komberg, Proc. Natl. Acad. Sci. U.S.A. 91, 1950 (1994); C. L. Peterson, A. Dingwall, M. P. Scott, ibid., p. 2905; J. Côté et al., Science 265, 53 (1994).
-
(1994)
Science
, vol.265
, pp. 53
-
-
Côté, J.1
-
8
-
-
0027493251
-
-
P. A. Khavari, C. L. Peterson, J. W. Tamkun, D. B. Mendel, G. R. Crabtree, Nature 366, 170 (1993); C. Muchardt and M. Yaniv, EMBO J. 12, 4279 (1993); H. Kwon et al., Nature 370, 477 (1994); A. N. Imbalzano et al., ibid., p. 481
-
(1993)
Nature
, vol.366
, pp. 170
-
-
Khavari, P.A.1
Peterson, C.L.2
Tamkun, J.W.3
Mendel, D.B.4
Crabtree, G.R.5
-
9
-
-
0027434083
-
-
P. A. Khavari, C. L. Peterson, J. W. Tamkun, D. B. Mendel, G. R. Crabtree, Nature 366, 170 (1993); C. Muchardt and M. Yaniv, EMBO J. 12, 4279 (1993); H. Kwon et al., Nature 370, 477 (1994); A. N. Imbalzano et al., ibid., p. 481
-
(1993)
EMBO J.
, vol.12
, pp. 4279
-
-
Muchardt, C.1
Yaniv, M.2
-
10
-
-
0028093378
-
-
P. A. Khavari, C. L. Peterson, J. W. Tamkun, D. B. Mendel, G. R. Crabtree, Nature 366, 170 (1993); C. Muchardt and M. Yaniv, EMBO J. 12, 4279 (1993); H. Kwon et al., Nature 370, 477 (1994); A. N. Imbalzano et al., ibid., p. 481
-
(1994)
Nature
, vol.370
, pp. 477
-
-
Kwon, H.1
-
11
-
-
0027493251
-
-
P. A. Khavari, C. L. Peterson, J. W. Tamkun, D. B. Mendel, G. R. Crabtree, Nature 366, 170 (1993); C. Muchardt and M. Yaniv, EMBO J. 12, 4279 (1993); H. Kwon et al., Nature 370, 477 (1994); A. N. Imbalzano et al., ibid., p. 481
-
Nature
, pp. 481
-
-
Imbalzano, A.N.1
-
12
-
-
0027068143
-
-
J. N. Hirschhorn, S. A. Brown, C. D. Clark, F. Winston, Genes Dev. 5, 2288 (1992).
-
(1992)
Genes Dev.
, vol.5
, pp. 2288
-
-
Hirschhorn, J.N.1
Brown, S.A.2
Clark, C.D.3
Winston, F.4
-
15
-
-
0023134783
-
-
C. D. Clark-Adams and F. Winston, Mol. Cell Biol. 7, 679 (1987); L. Neigeborn, J. L. Celenza, M. Carlson, ibid., p. 672; M. S. Swanson, M. Carlson, F. Winston, ibid. 10, 4935 (1990).
-
(1987)
Mol. Cell Biol.
, vol.7
, pp. 679
-
-
Clark-Adams, C.D.1
Winston, F.2
-
16
-
-
0023134783
-
-
C. D. Clark-Adams and F. Winston, Mol. Cell Biol. 7, 679 (1987); L. Neigeborn, J. L. Celenza, M. Carlson, ibid., p. 672; M. S. Swanson, M. Carlson, F. Winston, ibid. 10, 4935 (1990).
-
Mol. Cell Biol.
, pp. 672
-
-
Neigeborn, L.1
Celenza, J.L.2
Carlson, M.3
-
17
-
-
0025117930
-
-
C. D. Clark-Adams and F. Winston, Mol. Cell Biol. 7, 679 (1987); L. Neigeborn, J. L. Celenza, M. Carlson, ibid., p. 672; M. S. Swanson, M. Carlson, F. Winston, ibid. 10, 4935 (1990).
-
(1990)
Mol. Cell Biol.
, vol.10
, pp. 4935
-
-
Swanson, M.S.1
Carlson, M.2
Winston, F.3
-
18
-
-
15844379711
-
-
A. Bortvin and F. Winston, data not shown
-
A. Bortvin and F. Winston, data not shown.
-
-
-
-
19
-
-
15844396230
-
-
note
-
The isolation and analysis of yeast chromatin were done as previously described (6).
-
-
-
-
20
-
-
0023055514
-
-
M. A. Osley, J. Gould, S. Kim, M. Kane, L. Hereford, Cell 45, 537 (1986).
-
(1986)
Cell
, vol.45
, pp. 537
-
-
Osley, M.A.1
Gould, J.2
Kim, S.3
Kane, M.4
Hereford, L.5
-
22
-
-
0028794516
-
-
+ derivative of BJ2168 [E. Jones, in Methods Enzymol. 194, 428 (1991)]. Standard yeast genetic methods were used [F. Sherman, G. R. Fink, C. W. Lawrence, Methods in Yeast Genetics (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1978)].
-
(1995)
Yeast
, vol.11
, pp. 53
-
-
Winston, F.1
-
23
-
-
0025974219
-
-
+ derivative of BJ2168 [E. Jones, in Methods Enzymol. 194, 428 (1991)]. Standard yeast genetic methods were used [F. Sherman, G. R. Fink, C. W. Lawrence, Methods in Yeast Genetics (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1978)].
-
(1991)
Methods Enzymol.
, vol.194
, pp. 428
-
-
Jones, E.1
-
24
-
-
0003529274
-
-
Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
-
+ derivative of BJ2168 [E. Jones, in Methods Enzymol. 194, 428 (1991)]. Standard yeast genetic methods were used [F. Sherman, G. R. Fink, C. W. Lawrence, Methods in Yeast Genetics (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1978)].
-
(1978)
Methods in Yeast Genetics
-
-
Sherman, F.1
Fink, G.R.2
Lawrence, C.W.3
-
25
-
-
15844385463
-
-
note
-
119IIe) was PCR-amplified from genomic DNA of strain FY889, which contains this hht1 mutation. All PCR products were verified by sequencing. We determined the effect of histone overexpression on cell viability by shifting liquid cell cultures to the restrictive temperature (37°C) for 8 hours, after which cells were sonicated to disperse any clumps, dilutions were plated in duplicate, and plates were incubated at 30°C. Percent viability represents the fraction of viable cells at time zero that were able to grow after 8 hours of incubation at the restrictive temperature. Similar results were obtained when colony formation was monitored by light microscopy.
-
-
-
-
27
-
-
0028919756
-
-
A. Hecht, T. Laroche, S. Strahl-Bolsinger, S. M. Gasser, M. Grunstein, Cell 80, 583 (1995).
-
(1995)
Cell
, vol.80
, pp. 583
-
-
Hecht, A.1
Laroche, T.2
Strahl-Bolsinger, S.3
Gasser, S.M.4
Grunstein, M.5
-
30
-
-
0003903126
-
-
A. Rich, Ed. Springer-Verlag, New York
-
K. E. van Holde, Chromatin, A. Rich, Ed. (Springer-Verlag, New York, 1989).
-
(1989)
Chromatin
-
-
Van Holde, K.E.1
-
31
-
-
15844368494
-
-
note
-
3, 250 mM NaCl, and 1 mM dithiothreitol (DTT)] and the protease inhibitors chymostatin, leupeptin, pepstatin, benzamidine, and phenylmethylsulfonyl fluoride. For the column chromatography experiments, 10 mg of protein lysate was incubated with 0.5 ml of glutathione beads (Pharmacia) for 45 min in a final volume of 5 ml. The beads were then extensively washed with A250 and with A1000 (A250 with NaCl at 1 M).
-
-
-
-
33
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15844386966
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note
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Acid-extracted yeast histones were prepared from purified nuclei (6) as described (33), except that no butyrate was used in extraction buffers A and B, and extraction with 0.4 N sulfuric acid was done only once. The acid-soluble protein was precipitated in 20% trichloroacetic acid and the pellet was washed in acidified acetone, air-dried, and suspended in 10 mM tris-HCl (pH 8.0). Protein microsequencing was done at the Biopolymer Facility of the Howard Hughes Institute and the Department of Genetics, Harvard Medical School.
-
-
-
-
34
-
-
15844386585
-
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A. Bortvin, D. M. O'Rourke, F. Winston, data not shown
-
A. Bortvin, D. M. O'Rourke, F. Winston, data not shown.
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-
-
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35
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15844410747
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note
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GST and GST-Spt6p proteins used in far-western analysis were purified as described above (21), except that 1.5 ml of glutathione beads was used for every 10 mg of protein lysate. After the beads were washed with 30 ml of 50 mM tris-HCl (pH 7.5), 50 mM NaCl, and 1 mM DTT, the bound proteins were eluted in 2 ml of the same buffer plus 10 mM glutathione (Sigma) and concentrated on Centricon concentrators (Amicon). Far-western analysis was performed as described in the Fig. 3 legend.
-
-
-
-
36
-
-
0026297020
-
-
J. L. Workman, I. C. A. Taylor, R. E. Kingston, R. G. Roeder, Methods Cell Biol. 35, 419 (1991).
-
(1991)
Methods Cell Biol.
, vol.35
, pp. 419
-
-
Workman, J.L.1
Taylor, I.C.A.2
Kingston, R.E.3
Roeder, R.G.4
-
37
-
-
0021470855
-
-
Y. Ishimi et al., Eur. J. Biochem. 142, 431 (1984).; Y. Ishimi and A. Kikuchi, J. Biol. Chem. 266, 7025 (1991).
-
(1984)
Eur. J. Biochem.
, vol.142
, pp. 431
-
-
Ishimi, Y.1
-
38
-
-
0025816844
-
-
Y. Ishimi et al., Eur. J. Biochem. 142, 431 (1984).; Y. Ishimi and A. Kikuchi, J. Biol. Chem. 266, 7025 (1991).
-
(1991)
J. Biol. Chem.
, vol.266
, pp. 7025
-
-
Ishimi, Y.1
Kikuchi, A.2
-
39
-
-
0029618706
-
-
M. Bulger, T. Ito, R. Kamakaka, J. T. Kadonaga, Proc. Natl. Acad. Sci. U.S.A. 92, 11726 (1995).
-
(1995)
Proc. Natl. Acad. Sci. U.S.A.
, vol.92
, pp. 11726
-
-
Bulger, M.1
Ito, T.2
Kamakaka, R.3
Kadonaga, J.T.4
-
40
-
-
0027221804
-
-
K. Nishiwaki, T. Sano, J. Miwa, Mol. Gen. Genet. 239, 313 (1993); J. A. Segre et al., Genomics 28, 549 (1995).
-
(1993)
Mol. Gen. Genet.
, vol.239
, pp. 313
-
-
Nishiwaki, K.1
Sano, T.2
Miwa, J.3
-
41
-
-
0029149189
-
-
K. Nishiwaki, T. Sano, J. Miwa, Mol. Gen. Genet. 239, 313 (1993); J. A. Segre et al., Genomics 28, 549 (1995).
-
(1995)
Genomics
, vol.28
, pp. 549
-
-
Segre, J.A.1
-
42
-
-
15844368867
-
-
in press
-
G. A. Hartzog, M. A. Basrai, S. L. Ricupero-Hovasse, P. Hieter, F. Winston, Mol. Cell. Biol., in press.
-
Mol. Cell. Biol.
-
-
Hartzog, G.A.1
Basrai, M.A.2
Ricupero-Hovasse, S.L.3
Hieter, P.4
Winston, F.5
-
43
-
-
0004270170
-
-
Green Publishing/ Wiley Interscience, New York
-
All recombinant DNA manipulations were done by standard procedures [F. M. Ausubel et al., Current Protocols in Molecular Biology (Green Publishing/ Wiley Interscience, New York, 1988)].
-
(1988)
Current Protocols in Molecular Biology
-
-
Ausubel, F.M.1
-
45
-
-
0019402113
-
-
J. R. Davie, C. A. Saunders, J. M. Walsh, S. C. Weber, Nucleic Acids Res. 9, 3205 (1981).
-
(1981)
Nucleic Acids Res.
, vol.9
, pp. 3205
-
-
Davie, J.R.1
Saunders, C.A.2
Walsh, J.M.3
Weber, S.C.4
-
46
-
-
0019882018
-
-
W. Wray, T. Boulikas, V. P. Wray, R. Hancock, Anal. Biochem. 118, 197 (1981).
-
(1981)
Anal. Biochem.
, vol.118
, pp. 197
-
-
Wray, W.1
Boulikas, T.2
Wray, V.P.3
Hancock, R.4
-
47
-
-
15844369959
-
-
note
-
We are especially grateful to G. Hartzog for providing the GST-Spt6 fusion; to M. A. Osley for providing the Δ16′ promoter; and to S. Brown, T. Imbalzano, and R. Kingston for the human histones and frog extracts. We also thank E. Elion for the gift of pSYB305; S. Roth for advice on far-western analysis; J. Workman for valuable discussions; and A. Dudley, G. Hartzog, and R. Kingston for critical reading of the manuscript. Supported by NIH grant GM32967.
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