Transcription elongation factors repress transcription initiation from cryptic sites

Science

Volumn 301, Issue 5636, 2003, Pages 1096-1099

  Kaplan, Craig D ^a   Laprade, Lisa ^a   Winston, Fred ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENES; GENETIC ENGINEERING; RNA;

MUTANTS;

ENZYMES;

BACTERIAL PROTEIN; ELONGATION FACTOR; HISTONE H3; MUTANT PROTEIN; NUCLEASE; PROTEIN SPT6; RNA POLYMERASE II; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG;

RNA;

ARTICLE; CHROMATIN STRUCTURE; ENZYME ACTIVITY; GENE MUTATION; GENETIC CODE; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; SACCHAROMYCES CEREVISIAE; TRANSCRIPTION INITIATION;

CHROMATIN; GENE EXPRESSION REGULATION, FUNGAL; GENES, FUNGAL; HISTONES; MUTATION; NUCLEAR PROTEINS; NUCLEOSOMES; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PROMOTER REGIONS (GENETICS); RNA POLYMERASE II; RNA, FUNGAL; RNA, MESSENGER; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TATA-BOX BINDING PROTEIN; TRANS-ACTIVATORS; TRANSCRIPTION, GENETIC; TRANSCRIPTIONAL ELONGATION FACTORS;

BACTERIA (MICROORGANISMS); SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0041828953     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1087374     Document Type: Article

References (33)

1. G. Hartzog, J. Speer, D. Lindstrom, Biochim. Biophys. Acta 1577, 276 (2002).
2. G. A. Hartzog, T. Wada, H. Handa, F. Winston, Genes Dev. 12, 357 (1998).
3. E. D. Andrulis, E. Guzman, P. Doring, J. Werner, J. T. Lis, Genes Dev. 14, 2635 (2000).
4. E. D. Andrutis et al., Nature 420, 837 (2002).
5. C. D. Kaplan, J. R. Morris, C. Wu, F. Winston, Genes. Dev. 14, 2623 (2000).
6. M. S. Swanson, F. Winston, Genetics 132, 325 (1992).
7. E. A. Malone, C. D. Clark, A. Chiang, F. Winston, Mol. Cell. Biol. 11, 5710 (1991).
8. G. Natsoulis, F. Winston, J. D. Boeke, Genetics 136, 93 (1994).
9. C. Dollard, S. L. Ricupero-Hovasse, G. Natsoulis, J. D. Boeke, F. Winston, Mol. Cell. Biol. 14, 5223 (1994).
10. C. L. Denis, Genetics 108, 833 (1984).
11. G. Prelich, F. Winston, Genetics 135, 665 (1993).
12. D. L. Lindstrom, G. A. Hartzog, Genetics 159, 487 (2001).
13. N. J. Krogan et al., Mol. Cell. Biol. 22, 6979 (2002).
14. G. Orphanides, W. H. Wu, W. S. Lane, M. Hampsey, D. Reinberg, Nature 400, 284 (1999).
15. C. D. Kaplan, L. Laprade, F. Winston, unpublished observations.
16. A. Bortvin, F. Winston, Science 272, 1473 (1996).
17. M. Winkler, T. aus Dem Siepen, T. Stamminger, J. Virol. 74, 8053 (2000).
18. U. J. Kim, M. Han, P. Kayne, M. Grunstein, EMBO J. 7, 2211 (1988).
19. M. Han, M. Chang, U. J. Kim, M. Grunstein, Cell 48, 589 (1987).
20. D. Norris, B. Dunn, M. A. Osley, Science 242, 759 (1988).
21. M. Nonet, C. Scafe, J. Sexton, R. Young, Mol. Cell. Biol. 7, 1602 (1987).
22. T. Formosa et al., Genetics 162, 1557 (2002).
23. K. Adelman, J. T. Lis, Mol. Cell 9, 451 (2002).
24. R. H. Morse, Trends Biochem. Sci. 17, 23 (1992).
25. G. Orphanides, D. Reinberg, Nature 407, 471 (2000).
26. K. E. van Holde, D. E. Lohr, C. Robert, J. Biol. Chem. 267, 2837 (1992).
27. J. Gribnau, K. Diderich, S. Pruzina, R. Calzolari, P. Fraser, Mol. Cell 5, 377 (2000).
28. L. Neigeborn, J. L. Celenza, M. Carlson, Mol. Cell. Biol. 7, 672 (1987).
29. M. Han, M. Grunstein, Cell 55, 1137 (1988).
30. F. Winston, D. T. Chaleff, B. Valent, G. R. Fink, Genetics 107, 179 (1984).
31. C. D. Clark-Adams, F. Winston, Mol. Cell. Biol. 7, 679 (1987).
32. P. Komarnitsky, E. J. Cho, S. Buratowski, Genes Dev. 14, 2452 (2000).
33. We are indebted to J. Kaplan and B. Cairns for microarray experiments performed at the Huntsman Cancer Institute microarray facility. We thank S. Kaplan for data analysis and materials for RNA isolation; S. Buratowski for the gift of TBP-specific antibodies and discussions about 5′ RACE; A. Dudley for help with primer extension and sequencing analysis; E. Schwartzfarb for help with strain constructions; A. Dudley, G. Hartzog, and J. Wittmeyer for helpful comments on the manuscript; and Winston laboratory members, K. Struhl, and B. Meyer for helpful discussions. We thank an anonymous reviewer for suggesting the histone ChIP experiment. This work was supported by NIH grant GM32967 to F.W.