-
1
-
-
0029024475
-
Par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed
-
S. Guo, and K.J. Kemphues par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed Cell 81 1995 611 620
-
(1995)
Cell
, vol.81
, pp. 611-620
-
-
Guo, S.1
Kemphues, K.J.2
-
2
-
-
0032545933
-
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
-
A. Fire, S. Xu, M.K. Montgomery, S.A. Kostas, S.E. Driver, and C.C. Mello Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans Nature 391 1998 806 811
-
(1998)
Nature
, vol.391
, pp. 806-811
-
-
Fire, A.1
Xu, S.2
Montgomery, M.K.3
Kostas, S.A.4
Driver, S.E.5
Mello, C.C.6
-
3
-
-
0030829387
-
Wnt signaling and an APC-related gene specify endoderm in early C. elegans embryos
-
C.E. Rocheleau, W.D. Downs, R. Lin, C. Wittmann, Y. Bei, Y.H. Cha, M. Ali, J.R. Priess, and C.C. Mello Wnt signaling and an APC-related gene specify endoderm in early C. elegans embryos Cell 90 1997 707 716
-
(1997)
Cell
, vol.90
, pp. 707-716
-
-
Rocheleau, C.E.1
Downs, W.D.2
Lin, R.3
Wittmann, C.4
Bei, Y.5
Cha, Y.H.6
Ali, M.7
Priess, J.R.8
Mello, C.C.9
-
4
-
-
0032497694
-
The dynactin complex is required for cleavage plane specification in early Caenorhabditis elegans embryos
-
A.R. Skop, and J.G. White The dynactin complex is required for cleavage plane specification in early Caenorhabditis elegans embryos Curr Biol 8 1998 1110 1116
-
(1998)
Curr Biol
, vol.8
, pp. 1110-1116
-
-
Skop, A.R.1
White, J.G.2
-
5
-
-
0032497690
-
A nematode kinesin required for cleavage furrow advancement
-
J. Powers, O. Bossinger, D. Rose, S. Strome, and W. Saxton A nematode kinesin required for cleavage furrow advancement Curr Biol 8 1998 1133 1136
-
(1998)
Curr Biol
, vol.8
, pp. 1133-1136
-
-
Powers, J.1
Bossinger, O.2
Rose, D.3
Strome, S.4
Saxton, W.5
-
6
-
-
0031674842
-
Atypical protein kinase C cooperates with PAR-3 to establish embryonic polarity in Caenorhabditis elegans
-
Y. Tabuse, Y. Izumi, F. Piano, K.J. Kemphues, J. Miwa, and S. Ohno Atypical protein kinase C cooperates with PAR-3 to establish embryonic polarity in Caenorhabditis elegans Development 125 1998 3607 3614
-
(1998)
Development
, vol.125
, pp. 3607-3614
-
-
Tabuse, Y.1
Izumi, Y.2
Piano, F.3
Kemphues, K.J.4
Miwa, J.5
Ohno, S.6
-
7
-
-
0032443021
-
Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway
-
J.R. Kennerdell, and R.W. Carthew Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway Cell 95 1998 1017 1026
-
(1998)
Cell
, vol.95
, pp. 1017-1026
-
-
Kennerdell, J.R.1
Carthew, R.W.2
-
8
-
-
0034612276
-
Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways
-
J.C. Clemens, C.A. Worby, N. Simonson-Leff, M. Muda, T. Maehama, B.A. Hemmings, and J.E. Dixon Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways Proc Natl Acad Sci USA 97 2000 6499 6503
-
(2000)
Proc Natl Acad Sci USA
, vol.97
, pp. 6499-6503
-
-
Clemens, J.C.1
Worby, C.A.2
Simonson-Leff, N.3
Muda, M.4
Maehama, T.5
Hemmings, B.A.6
Dixon, J.E.7
-
9
-
-
0034737298
-
RNAi: Double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals
-
P.D. Zamore, T. Tuschl, P.A. Sharp, and D.P. Bartel RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals Cell 101 2000 25 33
-
(2000)
Cell
, vol.101
, pp. 25-33
-
-
Zamore, P.D.1
Tuschl, T.2
Sharp, P.A.3
Bartel, D.P.4
-
10
-
-
0035942736
-
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
-
S.M. Elbashir, J. Harborth, W. Lendeckel, A. Yalcin, K. Weber, and T. Tuschl Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells Nature 411 2001 494 498
-
(2001)
Nature
, vol.411
, pp. 494-498
-
-
Elbashir, S.M.1
Harborth, J.2
Lendeckel, W.3
Yalcin, A.4
Weber, K.5
Tuschl, T.6
-
11
-
-
0035859929
-
Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems
-
N.J. Caplen, S. Parrish, F. Imani, A. Fire, and R.A. Morgan Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems Proc Natl Acad Sci USA 98 2001 9742 9747
-
(2001)
Proc Natl Acad Sci USA
, vol.98
, pp. 9742-9747
-
-
Caplen, N.J.1
Parrish, S.2
Imani, F.3
Fire, A.4
Morgan, R.A.5
-
12
-
-
0036235167
-
RNAi-based functional genomics in C. elegans
-
F. Piano, and K.C. Gunsalus RNAi-based functional genomics in C. elegans Curr Genomics 3 2002 69 81
-
(2002)
Curr Genomics
, vol.3
, pp. 69-81
-
-
Piano, F.1
Gunsalus, K.C.2
-
13
-
-
5544315049
-
High-throughput RNAi in Caenorhabditis elegans: Genome-wide screens and functional genomics
-
A. Sugimoto High-throughput RNAi in Caenorhabditis elegans: genome-wide screens and functional genomics Differentiation 72 2004 81 91
-
(2004)
Differentiation
, vol.72
, pp. 81-91
-
-
Sugimoto, A.1
-
14
-
-
5544306760
-
Genome-wide high-throughput screens in functional genomics
-
A. Friedman, and N. Perrimon Genome-wide high-throughput screens in functional genomics Curr Opin Genet Dev 14 2004 470 476
-
(2004)
Curr Opin Genet Dev
, vol.14
, pp. 470-476
-
-
Friedman, A.1
Perrimon, N.2
-
15
-
-
0034676457
-
Functional genomic analysis of C. elegans chromosome I by systematic RNA interference
-
A.G. Fraser, R.S. Kamath, P. Zipperlen, M. Martinez-Campos, M. Sohrmann, and J. Ahringer Functional genomic analysis of C. elegans chromosome I by systematic RNA interference Nature 408 2000 325 330
-
(2000)
Nature
, vol.408
, pp. 325-330
-
-
Fraser, A.G.1
Kamath, R.S.2
Zipperlen, P.3
Martinez-Campos, M.4
Sohrmann, M.5
Ahringer, J.6
-
16
-
-
0034676448
-
Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III
-
P. Gönczy, C. Echeverri, K. Oegema, A. Coulson, S.J. Jones, R.R. Copley, J. Duperon, J. Oegema, M. Brehm, and E. Cassin Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III Nature 408 2000 331 336
-
(2000)
Nature
, vol.408
, pp. 331-336
-
-
Gönczy, P.1
Echeverri, C.2
Oegema, K.3
Coulson, A.4
Jones, S.J.5
Copley, R.R.6
Duperon, J.7
Oegema, J.8
Brehm, M.9
Cassin, E.10
-
17
-
-
0034649570
-
RNAi analysis of genes expressed in the ovary of Caenorhabditis elegans
-
F. Piano, A.J. Schetter, M. Mangone, L. Stein, and K.J. Kemphues RNAi analysis of genes expressed in the ovary of Caenorhabditis elegans Curr Biol 10 2000 1619 1622
-
(2000)
Curr Biol
, vol.10
, pp. 1619-1622
-
-
Piano, F.1
Schetter, A.J.2
Mangone, M.3
Stein, L.4
Kemphues, K.J.5
-
18
-
-
0035128859
-
Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi
-
I. Maeda, Y. Kohara, M. Yamamoto, and A. Sugimoto Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi Curr Biol 11 2001 171 176
-
(2001)
Curr Biol
, vol.11
, pp. 171-176
-
-
Maeda, I.1
Kohara, Y.2
Yamamoto, M.3
Sugimoto, A.4
-
19
-
-
0035125780
-
Functional genomics: The worm scores a knockout
-
S.K. Kim Functional genomics: the worm scores a knockout Curr Biol 11 2001 R85 R87
-
(2001)
Curr Biol
, vol.11
-
-
Kim, S.K.1
-
20
-
-
0037448540
-
Systematic functional analysis of the Caenorhabditis elegans genome using RNAi
-
R.S. Kamath, A.G. Fraser, Y. Dong, G. Poulin, R. Durbin, M. Gotta, A. Kanapin, N. Le Bot, S. Moreno, and M. Sohrmann Systematic functional analysis of the Caenorhabditis elegans genome using RNAi Nature 421 2003 231 237
-
(2003)
Nature
, vol.421
, pp. 231-237
-
-
Kamath, R.S.1
Fraser, A.G.2
Dong, Y.3
Poulin, G.4
Durbin, R.5
Gotta, M.6
Kanapin, A.7
Le Bot, N.8
Moreno, S.9
Sohrmann, M.10
-
21
-
-
1542298437
-
Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals novel gene functions
-
F. Simmer, C. Moorman, A.M. Van Der Linden, E. Kuijk, P.V. Van Den Berghe, R. Kamath, A.G. Fraser, J. Ahringer, and R.H. Plasterk Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals novel gene functions PLoS Biol 1 2003 E12
-
(2003)
PLoS Biol
, vol.1
-
-
Simmer, F.1
Moorman, C.2
Van Der Linden, A.M.3
Kuijk, E.4
Van Den Berghe, P.V.5
Kamath, R.6
Fraser, A.G.7
Ahringer, J.8
Plasterk, R.H.9
-
22
-
-
0043202980
-
A genome-wide screen identifies 27 genes involved in transposon silencing in C. elegans
-
N.L. Vastenhouw, S.E. Fischer, V.J. Robert, K.L. Thijssen, A.G. Fraser, R.S. Kamath, J. Ahringer, and R.H. Plasterk A genome-wide screen identifies 27 genes involved in transposon silencing in C. elegans Curr Biol 13 2003 1311 1316
-
(2003)
Curr Biol
, vol.13
, pp. 1311-1316
-
-
Vastenhouw, N.L.1
Fischer, S.E.2
Robert, V.J.3
Thijssen, K.L.4
Fraser, A.G.5
Kamath, R.S.6
Ahringer, J.7
Plasterk, R.H.8
-
23
-
-
0037443054
-
Identification of genes that protect the C. elegans genome against mutations by genome-wide RNAi
-
J. Pothof, G. van Haaften, K. Thijssen, R.S. Kamath, A.G. Fraser, J. Ahringer, R.H. Plasterk, and M. Tijsterman Identification of genes that protect the C. elegans genome against mutations by genome-wide RNAi Genes Dev 17 2003 443 448
-
(2003)
Genes Dev
, vol.17
, pp. 443-448
-
-
Pothof, J.1
Van Haaften, G.2
Thijssen, K.3
Kamath, R.S.4
Fraser, A.G.5
Ahringer, J.6
Plasterk, R.H.7
Tijsterman, M.8
-
24
-
-
0037448528
-
Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes
-
K. Ashrafi, F.Y. Chang, J.L. Watts, A.G. Fraser, R.S. Kamath, J. Ahringer, and G. Ruvkun Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes Nature 421 2003 268 272
-
(2003)
Nature
, vol.421
, pp. 268-272
-
-
Ashrafi, K.1
Chang, F.Y.2
Watts, J.L.3
Fraser, A.G.4
Kamath, R.S.5
Ahringer, J.6
Ruvkun, G.7
-
25
-
-
8844239144
-
Genome-wide RNAi identifies p53-dependent and -independent regulators of germ cell apoptosis in C. elegans
-
G. Lettre, E.A. Kritikou, M. Jaeggi, A. Calixto, A.G. Fraser, R.S. Kamath, J. Ahringer, and M.O. Hengartner Genome-wide RNAi identifies p53-dependent and -independent regulators of germ cell apoptosis in C. elegans Cell Death Differ 11 2004 1198 1203 This important analysis uses the Ahringer RNAi library [20] to identify genes involved in germline apoptosis and uses epistatic analysis to show that a large number of the genes identified rely on p53 activity.
-
(2004)
Cell Death Differ
, vol.11
, pp. 1198-1203
-
-
Lettre, G.1
Kritikou, E.A.2
Jaeggi, M.3
Calixto, A.4
Fraser, A.G.5
Kamath, R.S.6
Ahringer, J.7
Hengartner, M.O.8
-
26
-
-
3843064603
-
Caenorhabditis elegans WASP and Ena/VASP proteins play compensatory roles in morphogenesis and neuronal cell migration
-
J. Withee, B. Galligan, N. Hawkins, and G. Garriga Caenorhabditis elegans WASP and Ena/VASP proteins play compensatory roles in morphogenesis and neuronal cell migration Genetics 167 2004 1165 1176
-
(2004)
Genetics
, vol.167
, pp. 1165-1176
-
-
Withee, J.1
Galligan, B.2
Hawkins, N.3
Garriga, G.4
-
27
-
-
4444279075
-
Gene interactions in the DNA damage-response pathway identified by genome-wide RNA-interference analysis of synthetic lethality
-
G. van Haaften, N.L. Vastenhouw, E.A. Nollen, R.H. Plasterk, and M. Tijsterman Gene interactions in the DNA damage-response pathway identified by genome-wide RNA-interference analysis of synthetic lethality Proc Natl Acad Sci USA 101 2004 12992 12996 This important paper reports the first genome-scale analysis that uses the RNAi by feeding method in liquid culture, facilitating rapid large-scale screening using the existing Ahringer library [20]. This technology is used to identify several genes that are synthetically lethal with a mutation involved in double-stranded DNA repair or the chemical camptothecin, thus opening the door to synthetic screening on a scale previously performed only in yeast.
-
(2004)
Proc Natl Acad Sci USA
, vol.101
, pp. 12992-12996
-
-
Van Haaften, G.1
Vastenhouw, N.L.2
Nollen, E.A.3
Plasterk, R.H.4
Tijsterman, M.5
-
28
-
-
0037137443
-
Gene clustering based on RNAi phenotypes of ovary-enriched genes in C. elegans
-
F. Piano, A.J. Schetter, D.G. Morton, K.C. Gunsalus, V. Reinke, S.K. Kim, and K.J. Kemphues Gene clustering based on RNAi phenotypes of ovary-enriched genes in C. elegans Curr Biol 12 2002 1959 1964
-
(2002)
Curr Biol
, vol.12
, pp. 1959-1964
-
-
Piano, F.1
Schetter, A.J.2
Morton, D.G.3
Gunsalus, K.C.4
Reinke, V.5
Kim, S.K.6
Kemphues, K.J.7
-
29
-
-
0035422776
-
Roles for 147 embryonic lethal genes on C. elegans chromosome I identified by RNA interference and video microscopy
-
P. Zipperlen, A.G. Fraser, R.S. Kamath, M. Martinez-Campos, and J. Ahringer Roles for 147 embryonic lethal genes on C. elegans chromosome I identified by RNA interference and video microscopy EMBO J 20 2001 3984 3992
-
(2001)
EMBO J
, vol.20
, pp. 3984-3992
-
-
Zipperlen, P.1
Fraser, A.G.2
Kamath, R.S.3
Martinez-Campos, M.4
Ahringer, J.5
-
30
-
-
0345714887
-
Functional genomics of cell morphology using RNA interference: Pick your style, broad or deep
-
T.D. Pollard Functional genomics of cell morphology using RNA interference: pick your style, broad or deep J Biol 2 2003 25
-
(2003)
J Biol
, vol.2
, pp. 25
-
-
Pollard, T.D.1
-
31
-
-
0036329002
-
Molecular dissection of cytokinesis by RNA interference in Drosophila cultured cells
-
M.P. Somma, B. Fasulo, G. Cenci, E. Cundari, and M. Gatti Molecular dissection of cytokinesis by RNA interference in Drosophila cultured cells Mol Biol Cell 13 2002 2448 2460
-
(2002)
Mol Biol Cell
, vol.13
, pp. 2448-2460
-
-
Somma, M.P.1
Fasulo, B.2
Cenci, G.3
Cundari, E.4
Gatti, M.5
-
32
-
-
12344288635
-
Parallel chemical and genome-wide RNAi screens identify cytokinesis inhibitors and targets
-
in press.
-
Eggert US, Kiger AA, Richter C, Perlman ZE, Perrimon N, Mitchison TJ, Field CM: Parallel chemical and genome-wide RNAi screens identify cytokinesis inhibitors and targets. PLoS Biol 2004, in press. This impressive study combines genome-wide RNAi, carried out in triplicate, with testing of 51000 small molecules for their effect on cytokinesis. Very similar phenotypes were elicited by both methods, suggesting that RNAi and small molecule perturbations affect the same pathways. These observations further point to specific genes as potential molecular targets for the activity of small molecules.
-
(2004)
PLoS Biol
-
-
Eggert, U.S.1
Kiger, A.A.2
Richter, C.3
Perlman, Z.E.4
Perrimon, N.5
Mitchison, T.J.6
Field, C.M.7
-
33
-
-
4544374422
-
Terminal cytokinesis events uncovered after an RNAi screen
-
••]). It is noteworthy that the authors follow up on interesting candidates by using GFP markers and live time-lapse microscopy to probe the effect of the RNAi.
-
(2004)
Curr Biol
, vol.14
, pp. 1685-1693
-
-
Echard, A.1
Hickson, G.R.2
Foley, E.3
O'Farrell, P.H.4
-
34
-
-
0141433278
-
Molecular requirements for actin-based lamella formation in Drosophila S2 cells
-
S.L. Rogers, U. Wiedemann, N. Stuurman, and R.D. Vale Molecular requirements for actin-based lamella formation in Drosophila S2 cells J Cell Biol 162 2003 1079 1088
-
(2003)
J Cell Biol
, vol.162
, pp. 1079-1088
-
-
Rogers, S.L.1
Wiedemann, U.2
Stuurman, N.3
Vale, R.D.4
-
35
-
-
0141768258
-
The roles of microtubule-based motor proteins in mitosis: Comprehensive RNAi analysis in the Drosophila S2 cell line
-
G. Goshima, and R.D. Vale The roles of microtubule-based motor proteins in mitosis: comprehensive RNAi analysis in the Drosophila S2 cell line J Cell Biol 162 2003 1003 1016
-
(2003)
J Cell Biol
, vol.162
, pp. 1003-1016
-
-
Goshima, G.1
Vale, R.D.2
-
36
-
-
0242361514
-
A functional genomic analysis of cell morphology using RNA interference
-
A. Kiger, B. Baum, S. Jones, M. Jones, A. Coulson, C. Echeverri, and N. Perrimon A functional genomic analysis of cell morphology using RNA interference J Biol 2 2003 27
-
(2003)
J Biol
, vol.2
, pp. 27
-
-
Kiger, A.1
Baum, B.2
Jones, S.3
Jones, M.4
Coulson, A.5
Echeverri, C.6
Perrimon, N.7
-
37
-
-
0842309871
-
Genome-wide RNAi analysis of growth and viability in Drosophila cells
-
M. Boutros, A.A. Kiger, S. Armknecht, K. Kerr, M. Hild, B. Koch, S.A. Haas, H.F. Consortium, R. Paro, and N. Perrimon Genome-wide RNAi analysis of growth and viability in Drosophila cells Science 303 2004 832 835 This significant paper, which reports the first screen in D. melanogaster cell lines using an RNAi library covering nearly the entire genome, describes a high-throughput RNAi analysis using a rapid read-out to assay cell growth and viability. This study sets the stage for many future genome-scale screens in D. melanogaster cell lines.
-
(2004)
Science
, vol.303
, pp. 832-835
-
-
Boutros, M.1
Kiger, A.A.2
Armknecht, S.3
Kerr, K.4
Hild, M.5
Koch, B.6
Haas, S.A.7
Consortium, H.F.8
Paro, R.9
Perrimon, N.10
-
38
-
-
19344370696
-
Functional dissection of an innate immune response by a genome-wide RNAi screen
-
•]). The analysis uses a form of clustering based on the effect that RNAi treatments have on the expression level of a reporter gene designed to read the modulation of innate immunity. Importantly, it uses double-RNAi (i.e. simultaneous inhibition of two different genes) to probe epistatic relationships.
-
(2004)
PLoS Biol
, vol.2
-
-
Foley, E.1
O'Farrell, P.H.2
-
39
-
-
0037016444
-
Combined functional genomic maps of the C. elegans DNA damage response
-
S.J. Boulton, A. Gartner, J. Reboul, P. Vaglio, N. Dyson, D.E. Hill, and M. Vidal Combined functional genomic maps of the C. elegans DNA damage response Science 295 2002 127 131
-
(2002)
Science
, vol.295
, pp. 127-131
-
-
Boulton, S.J.1
Gartner, A.2
Reboul, J.3
Vaglio, P.4
Dyson, N.5
Hill, D.E.6
Vidal, M.7
-
40
-
-
10744231500
-
Systematic interactome mapping and genetic perturbation analysis of a C. elegans TGF-β signaling network
-
M. Tewari, P.J. Hu, J.S. Ahn, N. Ayivi-Guedehoussou, P.O. Vidalain, S. Li, S. Milstein, C.M. Armstrong, M. Boxem, and M.D. Butler Systematic interactome mapping and genetic perturbation analysis of a C. elegans TGF-β signaling network Mol Cell 13 2004 469 482 This paper reports the identification of new components of the TGF-β pathway by a combination of protein interaction mapping and RNAi. Importantly, this paper also establishes the epistatic relationships of newly discovered proteins with known pathway components.
-
(2004)
Mol Cell
, vol.13
, pp. 469-482
-
-
Tewari, M.1
Hu, P.J.2
Ahn, J.S.3
Ayivi-Guedehoussou, N.4
Vidalain, P.O.5
Li, S.6
Milstein, S.7
Armstrong, C.M.8
Boxem, M.9
Butler, M.D.10
-
41
-
-
0042092531
-
Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegans
-
C.T. Murphy, S.A. McCarroll, C.I. Bargmann, A. Fraser, R.S. Kamath, J. Ahringer, H. Li, and C. Kenyon Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegans Nature 424 2003 277 283
-
(2003)
Nature
, vol.424
, pp. 277-283
-
-
Murphy, C.T.1
McCarroll, S.A.2
Bargmann, C.I.3
Fraser, A.4
Kamath, R.S.5
Ahringer, J.6
Li, H.7
Kenyon, C.8
-
42
-
-
3042718729
-
Dissection of the mammalian midbody proteome reveals conserved cytokinesis mechanisms
-
A.R. Skop, H. Liu, J. Yates III, B.J. Meyer, and R. Heald Dissection of the mammalian midbody proteome reveals conserved cytokinesis mechanisms Science 305 2004 61 66 This important study combines the use of mass spectrometry to identify components of the mammalian midbody with RNAi of the homologues in C. elegans, where cytokinesis can be studied in living animals, and demonstrates that many of these proteins are required for completion of cytokinesis.
-
(2004)
Science
, vol.305
, pp. 61-66
-
-
Skop, A.R.1
Liu, H.2
Yates III, J.3
Meyer, B.J.4
Heald, R.5
-
43
-
-
0141569812
-
Interfering with disease: Opportunities and roadblocks to harnessing RNA interference
-
J. Lieberman, E. Song, S.K. Lee, and P. Shankar Interfering with disease: opportunities and roadblocks to harnessing RNA interference Trends Mol Med 9 2003 397 403
-
(2003)
Trends Mol Med
, vol.9
, pp. 397-403
-
-
Lieberman, J.1
Song, E.2
Lee, S.K.3
Shankar, P.4
-
44
-
-
3042704068
-
Optimizing RNA interference for application in mammalian cells
-
R.H. Medema Optimizing RNA interference for application in mammalian cells Biochem J 380 2004 593 603
-
(2004)
Biochem J
, vol.380
, pp. 593-603
-
-
Medema, R.H.1
-
45
-
-
3342903336
-
Challenges for RNAi in vivo
-
Z. Paroo, and D.R. Corey Challenges for RNAi in vivo Trends Biotechnol 22 2004 390 394
-
(2004)
Trends Biotechnol
, vol.22
, pp. 390-394
-
-
Paroo, Z.1
Corey, D.R.2
-
46
-
-
4644350944
-
Unlocking the potential of the human genome with RNA interference
-
G.J. Hannon, and J.J. Rossi Unlocking the potential of the human genome with RNA interference Nature 431 2004 371 378
-
(2004)
Nature
, vol.431
, pp. 371-378
-
-
Hannon, G.J.1
Rossi, J.J.2
-
47
-
-
12144289681
-
A large-scale RNAi screen in human cells identifies new components of the p53 pathway
-
••] describe similar approaches to high-throughput RNAi in mammalian cells. A library of 8000 human shRNAs is screened in pools to increase throughput and targets are identified using molecular barcodes.
-
(2004)
Nature
, vol.428
, pp. 431-437
-
-
Berns, K.1
Hijmans, E.M.2
Mullenders, J.3
Brummelkamp, T.R.4
Velds, A.5
Heimerikx, M.6
Kerkhoven, R.M.7
Madiredjo, M.8
Nijkamp, W.9
Weigelt, B.10
-
48
-
-
12144288006
-
A resource for large-scale RNA-interference-based screens in mammals
-
••] describe similar approaches to high-throughput RNAi in mammalian cells. An shRNA library against 10 000 human and 5000 mouse genes is screened in pools to increase throughput and targets affecting proteasome function are recovered using molecular barcodes. Multifunctional shRNA cassettes have been designed to allow shuttling of inserts to other vector systems.
-
(2004)
Nature
, vol.428
, pp. 427-431
-
-
Paddison, P.J.1
Silva, J.M.2
Conklin, D.S.3
Schlabach, M.4
Li, M.5
Aruleba, S.6
Balija, V.7
O'Shaughnessy, A.8
Gnoj, L.9
Scobie, K.10
-
49
-
-
0035799654
-
Microarrays of cells expressing defined cDNAs
-
J. Ziauddin, and D.M. Sabatini Microarrays of cells expressing defined cDNAs Nature 411 2001 107 110
-
(2001)
Nature
, vol.411
, pp. 107-110
-
-
Ziauddin, J.1
Sabatini, D.M.2
-
50
-
-
10744230287
-
RNAi microarray analysis in cultured mammalian cells
-
S. Mousses, N.J. Caplen, R. Cornelison, D. Weaver, M. Basik, S. Hautaniemi, A.G. Elkahloun, R.A. Lotufo, A. Choudary, and E.R. Dougherty RNAi microarray analysis in cultured mammalian cells Genome Res 13 2003 2341 2347
-
(2003)
Genome Res
, vol.13
, pp. 2341-2347
-
-
Mousses, S.1
Caplen, N.J.2
Cornelison, R.3
Weaver, D.4
Basik, M.5
Hautaniemi, S.6
Elkahloun, A.G.7
Lotufo, R.A.8
Choudary, A.9
Dougherty, E.R.10
-
51
-
-
0142092529
-
High-throughput selection of effective RNAi probes for gene silencing
-
R. Kumar, D.S. Conklin, and V. Mittal High-throughput selection of effective RNAi probes for gene silencing Genome Res 13 2003 2333 2340
-
(2003)
Genome Res
, vol.13
, pp. 2333-2340
-
-
Kumar, R.1
Conklin, D.S.2
Mittal, V.3
-
52
-
-
2342513372
-
RNA interference microarrays: High-throughput loss-of-function genetics in mammalian cells
-
J.M. Silva, H. Mizuno, A. Brady, R. Lucito, and G.J. Hannon RNA interference microarrays: high-throughput loss-of-function genetics in mammalian cells Proc Natl Acad Sci USA 101 2004 6548 6552 This study confirms and extends the above proof-of-principle reports [50,51] demonstrating the feasibility of printing siRNAs or shRNAs on glass slides for RNAi studies in mammalian cells.
-
(2004)
Proc Natl Acad Sci USA
, vol.101
, pp. 6548-6552
-
-
Silva, J.M.1
Mizuno, H.2
Brady, A.3
Lucito, R.4
Hannon, G.J.5
-
53
-
-
7444270371
-
Towards improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library
-
J.F. Rual, J. Ceron, J. Koreth, T. Hao, A.-S. Nicot, T. Hirozane-Kishikawa, J. Vandenhaute, Orkin Sh, D.E. Hill, and S. Van Den Heuvel Towards improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library Genome Res 14 2004 2162 2168 A new library based on the ORFeome for performing large-scale RNAi studies in C. elegans is described and tested.
-
(2004)
Genome Res
, vol.14
, pp. 2162-2168
-
-
Rual, J.F.1
Ceron, J.2
Koreth, J.3
Hao, T.4
Nicot, A.-S.5
Hirozane-Kishikawa, T.6
Vandenhaute, J.7
Orkin, Sh.8
Hill, D.E.9
Van Den Heuvel, S.10
-
54
-
-
0037137446
-
Integrating interactome, phenome, and transcriptome mapping data for the C. elegans germline
-
A.J. Walhout, J. Reboul, O. Shtanko, N. Bertin, P. Vaglio, H. Ge, H. Lee, L. Doucette-Stamm, K.C. Gunsalus, and A.J. Schetter Integrating interactome, phenome, and transcriptome mapping data for the C. elegans germline Curr Biol 12 2002 1952 1958
-
(2002)
Curr Biol
, vol.12
, pp. 1952-1958
-
-
Walhout, A.J.1
Reboul, J.2
Shtanko, O.3
Bertin, N.4
Vaglio, P.5
Ge, H.6
Lee, H.7
Doucette-Stamm, L.8
Gunsalus, K.C.9
Schetter, A.J.10
-
55
-
-
0037173615
-
Functional profiling of the Saccharomyces cerevisiae genome
-
G. Giaever, A.M. Chu, L. Ni, C. Connelly, L. Riles, S. Veronneau, S. Dow, A. Lucau-Danila, K. Anderson, and B. Andre Functional profiling of the Saccharomyces cerevisiae genome Nature 418 2002 387 391
-
(2002)
Nature
, vol.418
, pp. 387-391
-
-
Giaever, G.1
Chu, A.M.2
Ni, L.3
Connelly, C.4
Riles, L.5
Veronneau, S.6
Dow, S.7
Lucau-Danila, A.8
Anderson, K.9
Andre, B.10
-
56
-
-
0036923370
-
Damage recovery pathways in Drosophila revealed by genomic phenotyping and interactome mapping
-
T.J. Begley, A.S. Rosenbach, T. Ideker, and L.D. Samson Damage recovery pathways in Drosophila revealed by genomic phenotyping and interactome mapping Mol Cancer Res 1 2002 103 112
-
(2002)
Mol Cancer Res
, vol.1
, pp. 103-112
-
-
Begley, T.J.1
Rosenbach, A.S.2
Ideker, T.3
Samson, L.D.4
-
57
-
-
3142536716
-
Exploration of essential gene functions via titratable promoter alleles
-
S. Mnaimneh, A.P. Davierwala, J. Haynes, J. Moffat, W.T. Peng, W. Zhang, X. Yang, J. Pootoolal, G. Chua, and A. Lopez Exploration of essential gene functions via titratable promoter alleles Cell 118 2004 31 44 This beautiful study reports how titration of gene function can be used to study genes whose complete loss of function is lethal and therefore not very informative. By analyzing the phenotypes and transcription profiles of the titrated alleles, the authors show how this approach can decipher novel functions for this critical set of genes.
-
(2004)
Cell
, vol.118
, pp. 31-44
-
-
Mnaimneh, S.1
Davierwala, A.P.2
Haynes, J.3
Moffat, J.4
Peng, W.T.5
Zhang, W.6
Yang, X.7
Pootoolal, J.8
Chua, G.9
Lopez, A.10
-
58
-
-
10744230485
-
Global mapping of the yeast genetic interaction network
-
A.H. Tong, G. Lesage, G.D. Bader, H. Ding, H. Xu, X. Xin, J. Young, G.F. Berriz, R.L. Brost, and M. Chang Global mapping of the yeast genetic interaction network Science 303 2004 808 813 A genetic interaction network was derived in S. cerevisiae by crossing viable mutant strains and scoring the resulting double mutant progeny for fitness defects. Networks of functionally related genes were identified based on similar patterns of interactions. This work lays the foundation for understanding multigenic traits.
-
(2004)
Science
, vol.303
, pp. 808-813
-
-
Tong, A.H.1
Lesage, G.2
Bader, G.D.3
Ding, H.4
Xu, H.5
Xin, X.6
Young, J.7
Berriz, G.F.8
Brost, R.L.9
Chang, M.10
-
59
-
-
0347473810
-
Integration of chemical-genetic and genetic interaction data links bioactive compounds to cellular target pathways
-
A.B. Parsons, R.L. Brost, H. Ding, Z. Li, C. Zhang, B. Sheikh, G.W. Brown, P.M. Kane, T.R. Hughes, and C. Boone Integration of chemical-genetic and genetic interaction data links bioactive compounds to cellular target pathways Nat Biotechnol 22 2004 62 69
-
(2004)
Nat Biotechnol
, vol.22
, pp. 62-69
-
-
Parsons, A.B.1
Brost, R.L.2
Ding, H.3
Li, Z.4
Zhang, C.5
Sheikh, B.6
Brown, G.W.7
Kane, P.M.8
Hughes, T.R.9
Boone, C.10
-
60
-
-
9144264169
-
A map of the interactome network of the metazoan C. elegans
-
S. Li, C.M. Armstrong, N. Bertin, H. Ge, S. Milstein, M. Boxem, P.O. Vidalain, J.D. Han, A. Chesneau, and T. Hao A map of the interactome network of the metazoan C. elegans Science 303 2004 540 543 This paper describes the first large-scale mapping of protein interaction data for C. elegans. 4000 interactions focusing on metazoan-specific genes are identified using the yeast two-hybrid system. The overall quality of the dataset is confirmed using coaffinity purification.
-
(2004)
Science
, vol.303
, pp. 540-543
-
-
Li, S.1
Armstrong, C.M.2
Bertin, N.3
Ge, H.4
Milstein, S.5
Boxem, M.6
Vidalain, P.O.7
Han, J.D.8
Chesneau, A.9
Hao, T.10
-
61
-
-
0037847407
-
Differential activation of the DNA replication checkpoint contributes to asynchrony of cell division in C. elegans embryos
-
M. Brauchle, K. Baumer, and P. Gonczy Differential activation of the DNA replication checkpoint contributes to asynchrony of cell division in C. elegans embryos Curr Biol 13 2003 819 827
-
(2003)
Curr Biol
, vol.13
, pp. 819-827
-
-
Brauchle, M.1
Baumer, K.2
Gonczy, P.3
-
62
-
-
0347755544
-
RNAiDB and PhenoBlast: Web tools for genome-wide phenotypic mapping projects
-
K.C. Gunsalus, W.C. Yueh, P. MacMenamin, and F. Piano RNAiDB and PhenoBlast: web tools for genome-wide phenotypic mapping projects Nucleic Acids Res 32 Database issue 2004 D406 D410 This paper describes the first online database devoted to RNAi phenotypes and associated tools for analyzing and mining phenotypic data. Despite being implemented for C. elegans RNAi data, these resources are applicable to phenotypic data from other experimental systems.
-
(2004)
Nucleic Acids Res
, vol.32
-
-
Gunsalus, K.C.1
Yueh, W.C.2
MacMenamin, P.3
Piano, F.4
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