The Production and Application of Single-Chain Antibody Fragments

Folia Microbiologica

Volumn 48, Issue 5, 2003, Pages 687-698

  Blazek D ^a   Celer, V ^a  

^a UNIVERSITY OF VETERINARY AND PHARMACEUTICAL SCIENCES   (Czech Republic)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMALIA;

EID: 0347567461     PISSN: 00155632     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02993480     Document Type: Review

References (87)

1. ALVAREZ R.D., BARNES M.N., GOMEZ-NAVARRO J., WANG M., STRONG T.V., ARAFAT W., ARANI R.B., JOHNSON M.R., ROBERTS B.L., SIEGAL G.P., CURIEL D.T.: A cancer gene therapy approach utilizing an anti-erbB-2 single-chain antibody-encoding adenovirus (AD21): a phase I trial. Clin. Cancer Res. 6, 3081-3087 (2000).
2. ARBABI GHAHROUDI M., DESMYTER A., WYNS L., HAMERS R., MUYLDERMANS S.: Selection and identification of single domain antibody fragments from camel heavy-chain antibodies. FEBS Lett. 414, 521-526 (1997).
3. BAI J., SUI J., ZHU R.Y., TALLARICO A.S., GENNARI F., ZHANG D., MARASCO W.A.: Inhibition of Tat-mediated transactivation and HIV-1 replication by human anti-hCyclinT1 intrabodies. J. Biol. Chem. 278, 1433-1442 (2003).
4. BALTIMORE D.: Gene therapy. Intracellular immunization. Nature 335, 395-396 (1988).
5. BARBAS C.F. 3rd, KANG A.S., LERNER R.A., BENKOVIC S.J.: Assembly of combinatorial antibody libraries on phage surfaces: the gene III site. Proc. Nat. Acad. Sci. USA 88, 7978-7982 (1991).
6. BARBAS C.F. 3rd, COLLET T.A., AMBERG W., ROBEN P., BINLEY J.M., HOEKSTRA D., CABABA D., JONES T.M., WILLIAMSON R.A., PILKINGTON G.R., HAIGWOOD N.L., CABEZAS E., SATTERTHWAIT A.C., SANZ I., BURTON D.R.: Molecular profile of an antibody response to HIV-1 as probed by combinatorial libraries. J. Mol. Biol. 230, 812-823 (1993).
7. BEI R., SCHLOM J., KASHMIRI S.V.: Baculovirus expression of a functional single-chain immunoglobulin and its IL-2 fusion protein. J. Immunol. Meth. 186, 245-255 (1995).
8. BIOCCA S., PIERANDREI-AMALDI P., CAMPIONI N., CATTANEO A.: Intracellular immunization with cytosolic recombinant antibodies. Biotechnology (New York) 12, 396-399 (1994).
9. BIRD R.E., HARDMAN K.D., JACOBSON J.W., JOHNSON S., KAUFMAN B.M., LEE S.M., LEE T., POPE S.H., RIORDAN G.S., WHITLOW M.: Single-chain antigen-binding proteins. Science 242, 423-426 (1988).
10. BOUHAMDAN M., DUAN L.X., POMERANTZ R.J., STRAYER D.S.: Inhibition of HIV-1 by an anti-integrase single-chain variable fragment (SFv): delivery by SV40 provides durable protection against HIV-1 and does not require selection. Gene Ther. 6, 660-666 (1999).
11. BOUHAMDAN M., KULKOSKY J., DUAN L.X., POMERANTZ R.J.: Inhibition of HIV-1 replication and infectivity by expression of a fusion protein, VPR-anti-integrase single-chain variable fragment (SFv): intravirion molecular therapies. J.Human Virol. 3, 6-15 (2000).
12. BOUHAMDAN M., STRAYER D.S., WEI D., MUKHTAR M., DUAN L.X., HOXIE J., POMERANTZ R.J.: Inhibition of HIV-1 infection by down-regulation of the CXCR4 co-receptor using an intracellular single chain variable fragment against CXCR4. Gene Ther. 8, 408-418 (2001).
13. BROWN P.O., BOTSTEIN D.: Exploring the new world of the genome with DNA microarrays. Nature Genet. 21, 33-37 (1999).
14. CELER V., BLAŽEK D., NAVRÁTILOVÁ I., SKLÁDAL P., BLACKLAWS B., BUJDOSO R.: Recombinant single-chain Fv antibodies that recognize the p25 protein of the Maedi-Visna virus. Folia Microbiol. 48, 435-440 (2003).
15. CHARI R. V.: Targeted delivery of chemotherapeutics: tumor-activated prodrug therapy. Adv. Drug Deliv. Rev. 31, 89-104 (1998).
16. CHARLTON K., HARRIS W.J., PORTER A.J.: The isolation of super-sensitive anti-hapten antibodies from combinatorial antibody libraries derived from sheep. Biosens. Bioelectron. 16, 639-646 (2001).
17. CHEN S.Y., KHOURI Y., BAGLEY J., MARASCO W.A.: Combined intra-and extracellular immunization against human immunodeficiency virus type 1 infection with a human anti-gp120 antibody. Proc. Nat. Acad. Sci. USA 91, 5932-5936 (1994).
18. CLACKSON T., HOOGENBOOM H.R., GRIFFITHS A.D., WINTER G.: Making antibody fragments using phage display libraries. Nature 352, 624-628 (1991).
19. COCHET O., GRUEL N., FRIDMAN W.H., TEILLAUD J.L.: Ras and p53 intracellular targeting with recombinant single-chain Fv (scFv) fragments: a novel approach for cancer therapy? Cancer Detect. Prev. 23, 506-510 (1999).
20. CONRAD U., FIEDLER U.: Expression of engineered antibodies in plant cells. Plant Mol. Biol. 26, 1023-1030 (1994).
21. DAVIS G.T., BEDZYK W.D., VOSS E.W., JACOBS T.W.: Single chain antibody (SCA) encoding genes: one-step construction and expression in eukaryotic cells. Biotechnology (New York) 9, 165-169 (1991).
22. DUAN L., BAGASRA O., LAUGHLIN M.A., OAKES J.W., POMERANTZ R.J.: Potent inhibition of human immunodeficiency virus type 1 replication by an intracellular anti-Rev single-chain antibody. Proc. Nat. Acad. Sci. USA 91, 5075-5079 (1994).
23. FREYRE F.M., VAZQUEZ J.E., AYALA M., CANAAN-HADEN L., BELL H., RODRIGUEZ I., GONZALEZ A., CINTADO A., GAVILONDO J.V.: Very high expression of an anti-carcinoembryonic antigen single chain Fv antibody fragment in the yeast Pichia pastoris. J. Biotechnol. 76, 157-163 (2000).
24. GEORGE A.J., JAMAR F., TAI M.S., HEELAN B.T., ADAMS G.P., MCCARTNEY J.E., HOUSTON L.L., WEINER L.M., OPPERMANN H., PETERS A.M.: Radiometal labeling of recombinant proteins by a genetically engineered minimal chelation site: technetium-99m coordination by single-chain Fv antibody fusion proteins through a C-terminal cysteinyl peptide. Proc. Nat. Acad. Sci. USA 92, 8358-8362 (1995).
25. GONCALVES J., SILVA F., FREITAS-VIEIRA A., SANTA-MARTA M., MALHO R., YANG X., GABUZDA D., BARBAS C. 3rd: Functional neutralization of HIV-1 Vif protein by intracellular immunization inhibits reverse transcription and viral replication. J. Biol. Chem. 277, 32036-32045 (2002).
26. GRIEP R.A., VAN TWISK C., KERSCHBAUMER R.J., HARPER K., TORRANCE L., HIMMLER G., VAN DER WOLF J.M., SCHOTS A.: pSKAP/S: an expression vector for the production of single-chain Fv alkaline phosphatase fusion proteins. Prot. Exp. Purif. 16, 63-69 (1999a).
27. GRIEP R.A., VAN TWISK C., VAN DER WOLF J.M., SCHOTS A.: Fluobodies: green fluorescent single-chain Fv fusion proteins. J. Immunol. Meth. 230, 121-130 (1999b).
28. GRIEP R.A., PRINS M., VAN TWISK C., KELLER H.J.H.G., KERSCHBAUMER R.J., KORMELINK R., GOLDBACH R.W., SCHOTS A.: Application of phage display in selecting tomato spotted wilt virus-specific single-chain antibodies (scFvs) for sensitive diagnosis in ELISA. Phytopathology 90, 183-190 (2000).
29. GRIFFITHS A.D., DUNCAN A.R.: Strategies for selection of antibodies by phage display. Curr. Opin. Biotechnol. 9, 102-108 (1998).
30. GUIRAKHOO F., CATALAN J., MONATH T., WELTZIN R.: Cloning, expression and functional activities of a single chain antibody fragment directed to fusion protein of respiratory syncytial virus. Immunotechnology 2, 219-228 (1996).
31. DE HAARD H.J., KAZEMIER B., KOOLEN M.J., NIJHOLT L.J., MELOEN R.H., VAN GEMEN B., HOOGENBOOM H.R., ARENDS J.W.: Selection of recombinant, library-derived antibody fragments against p24 for human immunodeficiency virus type 1 diagnostics. Clin. Diagn. Lab. Immunol. 5, 636-644 (1998).
32. HANES J., PLUCKTHUN A.: In vitro selection and evolution of functional proteins by using ribosome display. Proc. Nat. Acad. Sci. USA 94, 4937-4942 (1997).
33. HARPER K., TOTH R.L., MAYO M.A., TORRANCE L.: Properties of a panel of single chain variable fragments against potato leafroll virus obtained from two phage display libraries. J. Virol. Meth. 81, 159-168 (1999).
34. HEINTGES T., ZU PUTLITZ J., WANDS J.R.: Characterization and binding of intracellular antibody fragments to the hepatitis C virus core protein. Biochem. Biophys. Res. Commun. 263, 410-418 (1999).
35. HOLT L.J., BUSSOW K., WALTER G., TOMLINSON I.M.: By-passing selection: direct screening for antibody-antigen interactions using protein arrays. Nucl. Acids Res. 28, E72 (2000).
36. HUDSON P.J., SOURIAU C.: Recombinant antibodies for cancer diagnosis and therapy. Expert. Opin. Biol. Ther. 1, 845-855 (2001).
37. HUST M., MAISS E., JACOBSEN H.J., REINARD T.: The production of a genus-specific recombinant antibody (scFv) using a recombinant potyvirus protease. J. Virol. Meth. 106, 225-233 (2002).
38. JOST C.R., KURUCZ I., JACOBUS C.M., TITUS J.A., GEORGE A.J., SEGAL D.M.: Mammalian expression and secretion of functional single-chain Fv molecules. J. Biol. Chem. 269, 26267-26273 (1994).
39. JURADO P., RITZ D., BECKWITH J., DE LORENZO V., FERNANDEZ L.A.: Production of functional single-chain Fv antibodies in the cytoplasm of Escherichia coli. J. Mol. Biol. 320, 1-10 (2002).
40. KALDERON D., RICHARDSON W.D., MARKHAM A.F., SMITH A.E.: Sequence requirements for nuclear location of simian virus 40 large-T antigen. Nature 311, 33-38 (1984).
41. KERSCHBAUMER R.J., HIRSCHL S., KAUFMANN A., IBL M., KOENIG R., HIMMLER G.: Single-chain Fv fusion proteins suitable as coating and detecting reagents in a double antibody sandwich enzyme-linked immunosorbent assay. Anal. Biochem. 249, 219-227 (1997).
42. KIPRIYANOV S.M., MOLDENHAUER G., LITTLE M.: High level production of soluble single chain antibodies in small-scale Escherichia coli cultures. J. Immunol. Meth. 200, 69-77 (1997).
43. KNAPPIK A., GE L., HONEGGER A., PACK P., FISCHER M., WELLNHOFER G., HOESS A., WOLLE J., PLUCKTHUN A., VIRNEKAS B.: Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides. J. Mol. Biol. 296, 57-86 (2000).
44. KOHLER G., MILSTEIN C.: Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256, 495-497 (1975).
45. KOZAK M.: At least six nucleotides preceding the AUG initiator codon enhance translation in mammalian cells. J. Mol. Biol. 196, 947-950 (1987).
46. KUROKI M., ARAKAWA F., KHARE P.D., KUROKI M., LIAO S., MATSUMOTO H., ABE H., IMAKIIRE T.: Specific targeting strategies of cancer gene therapy using a single-chain variable fragment (scFv) with a high affinity for CEA. Anticancer Res. 20, 4067-4071 (2000).
47. LEGASTELOIS I., DESGRANGES C.: Design and intracellular activity of a human single-chain antibody to human immunodeficiency virus type 1 conserved gp41 epitope. J. Virol. 74, 5712-5715 (2000).
48. LENER M., HORN I.R., CARDINALE A., MESSINA S., NIELSEN U.B., RYBAK S.M., HOOGENBOOM H.R., CATTANEO A., BIOCCA S.: Diverting a protein from its cellular location by intracellular antibodies. The case of p21Ras. Eur. J. Biochem. 267, 1196-1205 (2000).
49. LEVIN R., MHASHILKAR A.M., DORFMAN T., BUKOVSKY A., ZANI C., BAGLEY J., HINKULA J., NIEDRIG M., ALBERT J., WAHREN B., GOTTLINGER H.G., MARASCO W.A.: Inhibition of early and late events of the HIV-1 replication cycle by cytoplasmic Fab intrabodies against the matrix protein, p17. Mol. Med. 3, 96-110 (1997).
50. LEVY-MINTZ P., DUAN L., ZHANG H., HU B., DORNADULA G., ZHU M., KULKOSKY J., BIZUB-BENDER D., SKALKA A.M., POMERANTZ R.J.: Intracellular expression of single-chain variable fragments to inhibit early stages of the viral life cycle by targeting human immunodeficiency virus type 1 integrase. J. Virol. 70, 8821-8832 (1996).
51. LI X., STUCKERT P., BOSCH I., MARKS J.D., MARASCO W.A.: Single-chain antibody-mediated gene delivery into ErbB2-positive human breast cancer cells. Cancer Gene Ther. 8, 555-565 (2001).
52. LI Y., COLLINS M.S., WHITELAM G.C., ALEXANDER D.J.: Rapid pathotyping of Newcastle disease virus using a single-chain Fv displayed on phage against the C-terminal end of the F2 polypeptide. Arch. Virol. 147, 2025-2037 (2002).
53. LIU B., HUANG L., SIHLBOM C., BURLINGAME A., MARKS J.D.: Towards proteome-wide production of monoclonal antibody by phage display. J. Mol. Biol. 315, 1063-1073 (2002).
54. LIU S.Y., EARY J.F., PETERSDORF S.H., MARTIN P.J., MALONEY D.G., APPELBAUM F.R., MATTHEWS D.C., BUSH S.A., DURACK L.D., FISHER D.R., GOOLEY T.A., BERNSTEIN I.D., PRESS O.W.: Follow-up of relapsed B-cell lymphoma patients treated with iodine-131-labeled anti-CD20 antibody and autologous stem-cell rescue. J. Clin. Oncol. 16, 3270-3278 (1998).
55. MARASCO W.A.: Intrabodies: turning the humoral immune system outside in for intracellular immunization. Gene Ther. 4, 11-15 (1997).
56. MARASCO W.A., DANA JONES S.: Antibodies for targeted gene therapy: extracellular gene targeting and intracellular expression. Adv. Drug Deliv. Rev. 31, 153-170 (1998).
57. MARASCO W.A., HASELTINE W.A., CHEN S.Y.: Design, intracellular expression, and activity of a human anti-human immunodeficiency virus type 1 gp120 single-chain antibody. Proc. Nat. Acad. Sci. USA 90, 7889-7893 (1993).
58. MARASCO W.A., LAVECCHIO J., WINKLER A.: Human anti-HIV-1 tat sFv intrabodies for gene therapy of advanced HIV-1-infection and AIDS. J. Immunol. Meth. 231, 223-238 (1999).
59. MARIN M., PELEGRIN-ZURILLA M., BACHRACH E., NOEL D., BROCKLY F., PIECHACZYK M.: Antiviral activity of an intracellularly expressed single-chain antibody fragment directed against the murine leukemia virus capsid protein. Human Gene Ther. 11, 389-401 (2000).
60. MCCAFFERTY J., GRIFFITHS A.D., WINTER G., CHISWELL D.J.: Phage antibodies: filamentous phage displaying antibody variable domains. Nature 348, 552-554 (1990).
61. MHASHILKAR A.M., BISWAS D.K., LAVECCHIO J., PARDEE A.B., MARASCO W.A.: Inhibition of human immunodeficiency virus type 1 replication in vitro by a novel combination of anti-Tat single-chain intrabodies and NF-κB antagonists. J. Virol. 71, 6486-6494 (1997).
62. MORADPOUR D., BIECK E., HUGLE T., WELS W., WU J.Z., HONG Z., BLUM H.E., BARTENSCHLAGER R.: Functional properties of a monoclonal antibody inhibiting the hepatitis C virus RNA-dependent RNA polymerase. J. Biol. Chem. 277, 593-601 (2002).
63. MORINO K., KATSUMI H., AKAHORI Y., IBA Y., SHINOHARA M., UKAI Y., KOHARA Y., KUROSAWA Y.: Antibody fusions with fluorescent proteins: a versatile reagent for profiling protein expression. J. Immunol. Meth. 257, 175-184 (2001).
64. MULLER B.H., LAFAY F., DEMANGEL C., PERRIN P., TORDO N., FLAMAND A., LAFAYE P., GUESDON J.L.: Phage-displayed and soluble mouse scFv fragments neutralize rabies virus. J. Virol. Meth. 67, 221-233 (1997).
65. MUNRO S., PELHAM H.R.: A C-terminal signal prevents secretion of luminal ER proteins. Cell 48, 899-907 (1987).
66. NEJATOLLAHI F., HODGETTS S.J., VALLELY P.J., BURNIE J.P.: Neutralizing human recombinant antibodies to human cytomegalovirus glycoproteins gB and gH. FEMS Immunol. Med. Microbiol. 34, 237-244 (2002).
67. OELSCHLAEGER P., SRIKANT-IYER S., LANGE S., SCHMITT J., SCHMID R.: Fluorophor-linked immunosorbent assay: a time-and cost-saving method for the characterization of antibody fragments using a fusion protein of a single-chain antibody fragment and enhanced green fluorescent protein. Anal. Biochem. 309, 27-34 (2002).
68. OWEN M., GANDECHA A., COCKBURN B., WHITELAM G.: Synthesis of a functional antiphytochrome single-chain-Fv protein in transgenic tobacco. Biotechnology 10, 790-794 (1992).
69. PAI L.H., PASTAN I.: Clinical trials with Pseudomonas exotoxin immunotoxins. Curr. Top. Microbiol. Immunol. 234, 83-96 (1998).
70. PARMLEY S.F., SMITH G.P.: Antibody-selectable filamentous fd phage vectors: affinity purification of target genes. Gene 73, 305-318 (1988).
71. PLUCKTHUN A., SKERRA A.: Expression of functional antibody Fv and Fab fragments in Escherichia coli. Meth. Enzymol. 178, 497-515 (1989).
72. RIDDER R., GEISSE S., KLEUSER B., KAWALLECK P., GRAM H.: A COS-cell-based system for rapid production and quantification of scFv::IgC-κ antibody fragments. Gene 166, 273-276 (1995).
73. RONDON I.J., MARASCO W.A.: Intracellular antibodies (intrabodies) for gene therapy of infectious diseases. Ann. Rev. Microbiol. 51, 257-283 (1997).
74. SHAHEEN F., DUAN L., ZHU M., BAGASRA O., POMERANTZ R.J.: Targeting human immunodeficiency virus type 1 reverse transcriptase by intracellular expression of single-chain variable fragments to inhibit early stages of the viral life cycle. J. Virol. 70, 3392-3400 (1996).
75. SHEETS M.D., AMERSDORFER P., FINNERN R., SARGENT P., LINDQUIST E., SCHIER R., HEMINGSEN G., WONG C., GERHART J.C., MARKS J.D., LINDQVIST E.: Efficient construction of a large nonimmune phage antibody library: the production of high-affinity human single-chain antibodies to protein antigens. Proc. Nat. Acad. Sci. USA 95, 6157-6162 (1998).
76. SKERRA A.: Bacterial expression of immunoglobulin fragments. Curr. Opin. Immunol. 5, 256-262 (1993).
77. SKERRA A., PLUCKTHUN A.: Assembly of a functional immunoglobulin Fv fragment in Escherichia coli. Science 240, 1038-1041 (1988).
78. STEINBERGER P., ANDRIS-WIDHOPF J., BUHLER B., TORBETT B.E., BARBAS C.F. 3rd: Functional deletion of the CCR5 receptor by intracellular immunization produces cells that are refractory to CCR5-dependent HIV-1 infection and cell fusion. Proc. Nat. Acad. Sci. USA 97, 805-810 (2000).
79. STRAYER D.S., BRANCO F., LANDRE J., BOUHAMDAN M., SHAHEEN F., POMERANTZ R.J.: Combination genetic therapy to inhibit HIV-1. Mol. Ther. 5, 33-41 (2002).
80. STRUBE R.W., CHEN S.Y.: Characterization of anti-cyclin E single-chain Fv antibodies and intrabodies in breast cancer cells: enhanced intracellular stability of novel sFv-F(c) intrabodies. J. Immunol. Meth. 263, 149-167 (2002).
81. SULLIVAN D.E., MONDELLI M.U., CURIEL D.T., KRASNYKH V., MIKHEEVA G., GAGLIO P., MORRIS C.B., DASH S., GERBER M.A.: Construction and characterization of an intracellular single-chain human antibody to hepatitis C virus non-structural 3 protein. J. Hepatol. 37, 660-668 (2002).
82. TAVLADORAKI P., BENVENUTO E., TRINCA S., DE MARTINIS D., CATTANEO A., GALEFFI P.: Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack. Nature 366, 469-472 (1993).
83. THOR STRATEN P., GULDBERG P., SCHRAMA D., ANDERSEN M.H., MOERCH U., SEREMET T., SIEDEL C., REISFELD R.A., BECKER J.C.: In situ cytokine therapy: redistribution of clonally expanded T cells. Eur. J. Immunol. 31, 250-258 (2001).
84. VERMA R., BOLETI E., GEORGE A.J.: Antibody engineering: comparison of bacterial, yeast, insect and mammalian expression systems. J. Immunol. Meth. 216, 165-181 (1998).
85. DE WILDT R.M., MUNDY C.R., GORICK B.D., TOMLINSON I.M.: Antibody arrays for high-throughput screening of antibody-antigen interactions. Nat. Biotechnol. 18, 989-994 (2000).
86. WU Y., DUAN L., ZHU M., HU B., KUBOTA S., BAGASRA O., POMERANTZ R.J.: Binding of intracellular anti-Rev single chain variable fragments to different epitopes of human immunodeficiency virus type 1 rev: variations in viral inhibition. J. Virol. 70, 3290-3297 (1996).
87. ZHOU Y.H., TAKEKOSHI M., MAEDA F., IHARA S., ESUMI M.: Recombinant antibody Fab against the hypervariable region 1 of hepatitis C virus blocks the virus adsorption to susceptible cells in vitro. Antiviral Res. 56, 51-59 (2002).