Design and intracellular activity of a human single-chain antibody to human immunodeficiency virus type 1 conserved gp41 epitope

Journal of Virology

Volumn 74, Issue 12, 2000, Pages 5712-5715

  Legastelois, Isabelle ^a,b   Desgranges, Claude ^a,c  

^a INSERM   (France)

^b Hôpital St Vincent de Paul ^*  (France)

^c HÔPITAL SAINT VINCENT DE PAUL   (France)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCOPROTEIN GP 160; PLASMID DNA; VIRUS ANTIBODY;

ANTIBODY PRODUCTION; ANTIGEN RECOGNITION; ARTICLE; DIAGNOSTIC VALUE; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; LYMPHOID CELL; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; VIRUS NEUTRALIZATION;

CELL LINE; CONSERVED SEQUENCE; CYTOPLASM; EPITOPES; HIV ANTIBODIES; HIV ENVELOPE PROTEIN GP160; HIV ENVELOPE PROTEIN GP41; HIV-1; HUMANS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN VARIABLE REGION; NEUTRALIZATION TESTS; PROTEIN ENGINEERING; RNA, MESSENGER; TRANSFECTION; TUMOR CELLS, CULTURED; VIRUS REPLICATION;

EID: 0034074811     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.74.12.5712-5715.2000     Document Type: Article

References (33)

1. Barsov, E. V., W. E. Huber, J. Marcotrigiano, P. K. Clark, A. D. Clark, E. Arnold, and S. H. Hughes. 1996. Inhibition of human immunodeficiency virus type 1 integrase by the Fab fragment of a specific monoclonal antibody suggests that different multimerization states are required for different enzymatic functions. J. Virol. 70:4484-4494.
2. Cartier, C., P. Sivard, C. Tranchat, D. Decimo, C. Desgranges, and V. Boyer. 1999. Identification of three major phosphorylation sites within HIV-1 capsid: role of phosphorylation during the early steps of infection. J. Biol. Chem. 274:19434-19440.
3. Chen, J. D., Q. Yang, A. G. Yang, W. A. Marasco, and S. Y. Chen. 1996. Intra-and extracellular immunization against HIV-1 infection with lymphocytes transduced with an AAV vector expressing a human anti-gp120 antibody. Hum. Gene Ther. 7:1515-1525.
4. Chen, S. Y., J. Bagley, and W. A. Marasco. 1994. Intracellular antibodies as a new class of therapeutic molecules for gene therapy. Hum. Gene Ther. 5:595-601.
5. Chen, S. Y., Y. Khouri, J. Bagley, and W. A. Marasco. 1994. Combined intra-and extracellular immunization against human immunodeficiency virus type 1 infection with a human anti-gp120 antibody. Proc. Natl. Acad. Sci. USA 91:5932-5936.
6. David, D., and M. Zouali. 1995. Variable region light chain genes encoding human antibodies to HIV-1. Mol. Immunol. 32:77-88.
7. David, D., D. Goossens, C. Desgranges, J. Thèze, and M. Zouali. 1995. Molecular characterization of human monoclonal antibodies specific for several HIV proteins: analysis of the VH3 family expression. Immunol. Lett. 47:107-112.
8. Deng, H., R. Liu, W. Ellmeier, S. Choe, D. Unutmaz, M. Burkhart, S. Marmon, R. E. Sutton, C. M. Hill, C. B. David, S. C. Peiper, T. J. Schall, D. R. Littman, and N. R. Landau. 1996. Identification of a major co-receptor for primary isolates of HIV-1. Nature 381:661-666.
9. Duan, L., M. Zhu, O. Bagasra, and R. J. Pomerantz. 1995. Intracellular immunization against HIV-1 infection of human T lymphocytes: utility of anti-Rev single chain variable fragments. Hum. Gene Ther. 6:1561-1573.
10. Duan, L., H. Zhang, J. W. Oakes, O. Bagasra, and R. J. Pomerantz. 1994. Molecular and virological effects of intracellular anti-Rev single-chain variable fragments on the expression of various human immunodeficiency virus-1 strains. Hum. Gene Ther. 5:1315-1324.
11. Duan, L. K., O. Bagasra, M. A. Laughlin, J. W. Oakes, and R. J. Pomerantz. 1994. Potent inhibition of human immunodeficiency virus type 1 replication by intracellular anti-Rev single chain antibody. Proc. Natl. Acad. Sci. USA 91:5075-5079.
12. Ho, W. Z., J. P. Lai, M. Bouhamdan, L. Duan, R. J. Pomerantz, and S. E. Starr. 1998. Inhibition of HIV type 1 replication in chronically infected monocytes and lymphocytes by retrovirus-mediated gene transfer of anti-rev single-chain variable fragments. AIDS Res. Hum. Retroviruses 14:1573-1580.
13. Inouye, R. T., B. Du, D. Boldt-Houle, A. Ferrante, I. O. Park, S. M. Hamer, L. Duan, J. E. Groopman, R. J. Pomerantz, and F. Terwilliger. 1997. Potent inhibition of human immunodeficiency virus type 1 in primary T cells and alveolar macrophages by combination anti-Rev strategy delivered in adeno-associated virus vector. J. Virol. 71:4071-4078.
14. Junker, U., C. S. Kalfoglou, J. J. Moon, M. K. Beck, H. Kaneshima, and E. Böhnlein. 1998. Inhibition of human immunodeficiency virus type 1 replication in myelomonocytic cells derived from retroviral vector-transduced peripheral blood progenitor cells. Hum. Gene Ther. 9:333-340.
15. Kitamura, Y., T. Ishikawa, N. Okui, N. Kobayashi, T. Kanda, T. Shimada, K. Miyake, and K. Yoshiike. 1999. Inhibition of replication of HIV-1 at both early and late stages of the viral life cycle by single-chain antibody against integrase. J. Acquired Immune Defic. Syndr. Hum. Retrovirol. 20:105-114.
16. Kozak, M. 1986. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44:283-292.
17. Kozak, M. 1987. At least six nucleotides preceding the AUG initiator codon enhance translation in mammalian cells. J. Mol. Biol. 196:947-950.
18. Landau, N. L., and D. R. Littman. 1992. Packaging system for rapid production of murine leukemia virus vectors with variable tropism. J. Virol. 66:5110-5113.
19. Levy-Mintz, P., L. Duan, H. Zhang, B. Hu, G. Dornadula, M. Zhu, J. Kulkosky, D. Bizub-Bender, A. M. Skalka, and R. J. Pomerantz. 1996. Intracellular expression of single-chain variable fragments to inhibit early stages of the viral life cycle by targeting human immunodeficiency virus type 1 integrase. J. Virol. 70:8821-8832.
20. Marasco, W. A. 1997. Intrabodies: turning the humoral immune system outside in for intracellular immunization. Gene Ther. 4:11-15.
21. Marasco, W. A., W. A. Haseltine, and S. Y. Chen. 1993. Design, intracellular expression, and activity of a human anti-human immunodeficiency virus type 1 gp120 single-chain antibody. Proc. Natl. Acad. Sci. USA 90:7889-7893.
22. Mhashilkar, A. M., D. K. Biswas, J. L. Vecchio, A. B. Pardee, and W. A. Marasco. 1997. Inhibition of human immunodeficiency virus type 1 replication in vitro by a novel combination of anti-Tat single-chain intrabodies and NF-κB antagonists. J. Virol. 71:6486-6494.
23. Mhashilkar, A. M., J. Bagley, S. Y. Chen, A. M. Szilvay, D. G. Helland, and W. A. Marasco. 1995. Inhibition of HIV-1 infection by anti-Tat single chain intrabodies. EMBO J. 14:1542-1551.
24. Modow, S., B. H. Hahn, G. M. Shaw, R. C. Gallo, F. Wong-Staal, and H. Wolf. 1997. Computer-assisted analysis of envelope protein sequences of seven human immunodeficiency virus isolates: prediction of antigenic epitopes in conserved and variable regions. J. Virol. 61:570-578.
25. Myers, G., S. Wain-Hobson, L. E. Henderson, B. Korber, K. T. Jeang, and G. N. Pavlakis. 1997. Human retroviruses and AIDS. Theoretical Biology and Biophysics Group, Los Alamos National Laboratory. Los Alamos, N.Mex.
26. Gargano, S. Biocca, A. Bradbury, and A. Cattaneo. 1996. Human recombinant antibody fragments neutralizing human immunodeficiency virus type 1 reverse transcriptase provide an experimental basis for the structural classification of the DNA polymerase family. J. Virol. 70:7706-7712.
27. Nilsen, B. M., I. R. Haugan, K. Berg, L. Olsen, P. O. Brown, and D. E. Helland. 1996. Monoclonal antibodies against human immunodeficiency virus type 1 integrase: epitope mapping and differential effects on integrase activities in vitro. J. Virol. 70:1580-1587.
28. Peer, Y. V., W. Janssens, L. Heyndrickx, K. Fransen, G. Groen, and Rupert De Wachter. 1996. Phylogenetic analysis of the env gene of HIV-1 isolates taking into account individual nucleotide substitution rates. AIDS 10:1485-1494.
29. Rondon, I. J., and W. A. Marasco. 1997. Intracellular antibodies (intrabodies) for gene therapy of infectious disease. Annu. Rev. Microbiol. 51:257-283.
30. Shaheen, F., L. Duan, M. Zhu, O. Bagasra, and R. J. Pomerantz. 1996 Targeting human immunodeficiency virus type 1 reverse transcriptase by intracellular expression of single-chain variable fragments to inhibit early stages of viral life cycle. J. Virol. 70:3392-3400.
31. Tewari, D., S. L. Goldstein, A. L. Notkins, and P. Zhou. 1998. cDNA encoding a single-chain antibody to HIV p17 with cytoplasmic or nuclear retention signals inhibits HIV-1 replication. J. Immunol. 161:2642-2647.
32. Wu, Y., L. Duan, M. Zhu, B. Hu, S. Kubota, O. Bagasra, and R. J. Pomerantz. 1996. Binding of intracellular anti-Rev single chain variable fragments to different epitopes of human immunodeficiency virus type 1 Rev: variations in viral inhibition. J. Virol. 70:3290-3297.
33. Zhou, P., S. Goldstein, K. Devadas, D. Tewari, and A. L. Notkins. 1998 Cells transfected with a non-neutralizing antibody gene are resistant to HIV infection: targeting the endoplasmic reticulum and trans-Golgi network. J. Immunol. 160:1489-1496.