Fluorophor-linked immunosorbent assay: A time- and cost-saving method for the characterization of antibody fragments using a fusion protein of a single-chain antibody fragment and enhanced green fluorescent protein

Analytical Biochemistry

Volumn 309, Issue 1, 2002, Pages 27-34

  Oelschlaeger, Peter ^a   Srikant Iyer, Srividhya ^a   Lange, Stefan ^a   Schmitt, Jutta ^a   Schmid, Rolf D ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

Author keywords

EGFP; ELISA; Fluorescence immunoassay; Fusion protein; Online monitoring; s Triazine; scFv

Indexed keywords

ANTIBODIES; ENZYMES; ESCHERICHIA COLI; FLUORESCENCE; POLYOLS;

EGFP; ELISA; FLUORESCENCE IMMUNOASSAY; FUSION PROTEINS; ONLINE MONITORING; S-TRIAZINES; SCFV;

RECOMBINANT PROTEINS;

ANTIBODY; GREEN FLUORESCENT PROTEIN; HAPTEN; HYBRID PROTEIN; RECOMBINANT PROTEIN; TRIAZINE DERIVATIVE; IMMUNOGLOBULIN FRAGMENT; PHOTOPROTEIN; PRIMER DNA; SCFV GREEN FLUORESCENT PROTEIN, RECOMBINANT;

ANALYTIC METHOD; ARTICLE; COST CONTROL; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FLUORESCENCE; MONITORING; PRIORITY JOURNAL; PROTEIN DOMAIN; SCREENING; TIME; ANTIBODY SPECIFICITY; CALIBRATION; CHEMISTRY; FLUOROMETRY; GENETICS; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; PROTEIN FOLDING;

ESCHERICHIA COLI;

ANTIBODY SPECIFICITY; CALIBRATION; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FLUOROMETRY; GREEN FLUORESCENT PROTEINS; HAPTENS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOSORBENT TECHNIQUES; LUMINESCENT PROTEINS; PROTEIN BINDING; PROTEIN FOLDING; RECOMBINANT FUSION PROTEINS; TRIAZINES;

EID: 0036806997     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00290-7     Document Type: Article

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