-
1
-
-
0036498752
-
Functional cell-based uHTS in chemical genomic drug discovery
-
Croston GE: Functional cell-based uHTS in chemical genomic drug discovery. Trends Biotechnol 2002;3:110-115.
-
(2002)
Trends Biotechnol
, vol.3
, pp. 110-115
-
-
Croston, G.E.1
-
2
-
-
0030941983
-
New methods to mimic nature in high-throughput screening
-
Okun I, Veerapandian P: New methods to mimic nature in high-throughput screening. Nat Biotechnol 1997;15:287-288.
-
(1997)
Nat Biotechnol
, vol.15
, pp. 287-288
-
-
Okun, I.1
Veerapandian, P.2
-
3
-
-
85084247357
-
High-throughput screening for drug discovery
-
Broach JR, Thorner J: High-throughput screening for drug discovery. Nature 1996;384:14-16.
-
(1996)
Nature
, vol.384
, pp. 14-16
-
-
Broach, J.R.1
Thorner, J.2
-
4
-
-
0038342510
-
The cellular revolution, Addressing drug discovery bottlenecks with cell based imaging technologies
-
Goodyer ID, Brophy G, Roquemore EP: The cellular revolution: Addressing drug discovery bottlenecks with cell based imaging technologies. JCLA 2002;25:294-304.
-
(2002)
JCLA
, vol.25
, pp. 294-304
-
-
Goodyer, I.D.1
Brophy, G.2
Roquemore, E.P.3
-
5
-
-
0035028126
-
Cell-based versus isolated target screening: How lucky do you feel?
-
Moore K, Rees S: Cell-based versus isolated target screening: how lucky do you feel? J Biomol Screen 2001;6:69-74.
-
(2001)
J Biomol Screen
, vol.6
, pp. 69-74
-
-
Moore, K.1
Rees, S.2
-
6
-
-
34548576414
-
Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea
-
Shimomura O, Johnson FH and Saiga Y: Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. J Cell Comp Physiol 1962;59:223-239.
-
(1962)
J Cell Comp Physiol
, vol.5
, pp. 223-239
-
-
Shimomura, O.1
Johnson, F.H.2
Saiga, Y.3
-
7
-
-
0026578325
-
Primary structure of the Aequorea victoria green-fluorescent protein
-
Prasher DC, Eckenrode VK, Ward WW, Prendergast FG, Cormier MJ: Primary structure of the Aequorea victoria green-fluorescent protein. Gene 1992;111:229-233.
-
(1992)
Gene
, vol.111
, pp. 229-233
-
-
Prasher, D.C.1
Eckenrode, V.K.2
Ward, W.W.3
Prendergast, F.G.4
Cormier, M.J.5
-
8
-
-
23444431611
-
Green fluorescent protein as a marker for gene expression
-
Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC: Green fluorescent protein as a marker for gene expression. Science 1994;263:802-805.
-
(1994)
Science
, vol.263
, pp. 802-805
-
-
Chalfie, M.1
Tu, Y.2
Euskirchen, G.3
Ward, W.W.4
Prasher, D.C.5
-
9
-
-
0028227833
-
Implications for bcd mRNA localization from spatial distribution of exu protein in Drosophila oogenesis
-
Wang S, Hazelrigg T: Implications for bcd mRNA localization from spatial distribution of exu protein in Drosophila oogenesis. Nature 1994;369:400-403.
-
(1994)
Nature
, vol.369
, pp. 400-403
-
-
Wang, S.1
Hazelrigg, T.2
-
10
-
-
0028832822
-
Understanding, improving and using green fluorescent proteins
-
Cubitt AB, Heim R, Adams SR, Boyd AE, Gross LA, Tsien RY: Understanding, improving and using green fluorescent proteins. Trends Biochem Sci 1995;11:448-455.
-
(1995)
Trends Biochem Sci
, vol.11
, pp. 448-455
-
-
Cubitt, A.B.1
Heim, R.2
Adams, S.R.3
Boyd, A.E.4
Gross, L.A.5
Tsien, R.Y.6
-
11
-
-
0028580734
-
Wavelength mutations, posttranslational autoxidation of green fluorescent protein
-
Heim R, Prasher DC, Tsien RY.: Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc Natl Acad Sci USA 1994;91:12501-12504.
-
(1994)
Proc Natl Acad Sci USA
, vol.91
, pp. 12501-12504
-
-
Heim, R.1
Prasher, D.C.2
Tsien, R.Y.3
-
12
-
-
0037140742
-
New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: Synthesis and biological applications
-
Adams SR, Campbell RE, Gross LA, Martin BR, Walkup GK, Yao Y, Llopis J, Tsien RY: New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: Synthesis and biological applications. J Am Chem Soc 2002;124:6063-6076.
-
(2002)
J Am Chem Soc
, vol.124
, pp. 6063-6076
-
-
Adams, S.R.1
Campbell, R.E.2
Gross, L.A.3
Martin, B.R.4
Walkup, G.K.5
Yao, Y.6
Llopis, J.7
Tsien, R.Y.8
-
13
-
-
0029909407
-
Optimized codon usage, chromophore mutations provide enhanced sensitivity with the green fluorescent protein
-
Yang TT, Cheng L, Kain SR: Optimized codon usage and chromophore mutations provide enhanced sensitivity with the green fluorescent protein. Nucl Acids Res 1996;24:4592-4593.
-
(1996)
Nucl Acids Res
, vol.24
, pp. 4592-4593
-
-
Yang, T.T.1
Cheng, L.2
Kain, S.R.3
-
14
-
-
0029847806
-
Crystal structure of the Aequorea victoria green fluorescent protein
-
Ormo M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ: Crystal structure of the Aequorea victoria green fluorescent protein. Science 1996;273:1392-1395.
-
(1996)
Science
, vol.273
, pp. 1392-1395
-
-
Ormo, M.1
Cubitt, A.B.2
Kallio, K.3
Gross, L.A.4
Tsien, R.Y.5
Remington, S.J.6
-
15
-
-
0032532156
-
Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein
-
Wachter RM, Elsliger MA, Kallio K, Hanson GT, Remington SJ: Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein. Structure 1998;6:1267-1277.
-
(1998)
Structure
, vol.6
, pp. 1267-1277
-
-
Wachter, R.M.1
Elsliger, M.A.2
Kallio, K.3
Hanson, G.T.4
Remington, S.J.5
-
16
-
-
0030843865
-
Crystal structure, photodynamic behavior of the blue emission variant Y66H/Y145F of green fluorescent protein
-
Wachter RM, King BA, Heim R, Kallio K, Tsien RY, Boxer SG, Remington SJ: Crystal structure and photodynamic behavior of the blue emission variant Y66H/Y145F of green fluorescent protein. Biochemistry 1997;36:9759-9765.
-
(1997)
Biochemistry
, vol.36
, pp. 9759-9765
-
-
Wachter, R.M.1
King, B.A.2
Heim, R.3
Kallio, K.4
Tsien, R.Y.5
Boxer, S.G.6
Remington, S.J.7
-
17
-
-
0020482348
-
Reversible denaturation of Aequorea green-fluorescent protein: Physical separation, characterization of the renatured protein
-
Ward WW, Bokman SH: Reversible denaturation of Aequorea green-fluorescent protein: Physical separation and characterization of the renatured protein. Biochemistry 1982;21:4535-4540.
-
(1982)
Biochemistry
, vol.21
, pp. 4535-4540
-
-
Ward, W.W.1
Bokman, S.H.2
-
18
-
-
0030953638
-
Structural basis for dual excitation, photoisomerization of the Aequorea victoria green fluorescent protein
-
Brejc K, Sixma TK, Kitts PA, Kain SR, Tsien RY, Ormo M, Remington SJ: Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein. Proc Natl Acad Sci USA 1997;94:2306-2311.
-
(1997)
Proc Natl Acad Sci USA
, vol.94
, pp. 2306-2311
-
-
Brejc, K.1
Sixma, T.K.2
Kitts, P.A.3
Kain, S.R.4
Tsien, R.Y.5
Ormo, M.6
Remington, S.J.7
-
19
-
-
0031885535
-
Green fluorescent protein as a noninvasive intracellular pH indicator
-
Kneen M, Farinas J, Li Y, Verkman AS: Green fluorescent protein as a noninvasive intracellular pH indicator. Biophys J 1998;74:1591-1599.
-
(1998)
Biophys J
, vol.74
, pp. 1591-1599
-
-
Kneen, M.1
Farinas, J.2
Li, Y.3
Verkman, A.S.4
-
20
-
-
0029757121
-
Ultra-fast excited state dynamics in green fluorescent protein: Multiple states and proton transfer
-
Chattoraj M, King BA, Bublitz GU, Boxer SG: Ultra-fast excited state dynamics in green fluorescent protein: Multiple states and proton transfer. Proc Natl Acad Sci USA 1996;93:8362-8367.
-
(1996)
Proc Natl Acad Sci USA
, vol.93
, pp. 8362-8367
-
-
Chattoraj, M.1
King, B.A.2
Bublitz, G.U.3
Boxer, S.G.4
-
21
-
-
0031134224
-
The structural basis for spectral variations in green fluorescent protein
-
Palm GJ, Zdanov A, Gaitanaris GA, Stauber R, Pavlakis GN, Wlodawer A: The structural basis for spectral variations in green fluorescent protein. Nat Struct Biol 1997;4:361-365.
-
(1997)
Nat Struct Biol
, vol.4
, pp. 361-365
-
-
Palm, G.J.1
Zdanov, A.2
Gaitanaris, G.A.3
Stauber, R.4
Pavlakis, G.N.5
Wlodawer, A.6
-
22
-
-
0031663369
-
The green fluorescent protein
-
Tsien RY: The green fluorescent protein. Annu Rev Biochem 1998;67:509-544.
-
(1998)
Annu Rev Biochem
, vol.67
, pp. 509-544
-
-
Tsien, R.Y.1
-
23
-
-
0032607587
-
Understanding structure-function relationships in the Aequorea victoria green fluorescent protein
-
Cubitt AB, Woollenweber LA, Heim R: Understanding structure-function relationships in the Aequorea victoria green fluorescent protein. Methods Cell Biol 1999;58:19-30.
-
(1999)
Methods Cell Biol
, vol.58
, pp. 19-30
-
-
Cubitt, A.B.1
Woollenweber, L.A.2
Heim, R.3
-
24
-
-
0033613235
-
Circular permutation, receptor insertion within green fluorescent proteins
-
Baird GS, Zacharias DA, Tsien RY: Circular permutation and receptor insertion within green fluorescent proteins. Proc Natl Acad Sci USA. 1999;96:11241-11246.
-
(1999)
Proc Natl Acad Sci USA
, vol.96
, pp. 11241-11246
-
-
Baird, G.S.1
Zacharias, D.A.2
Tsien, R.Y.3
-
25
-
-
0030452512
-
Mutations that suppress the thermosensitivity of green fluorescent protein
-
Siemering KR, Golbik R, Sever R, Haseloff J: Mutations that suppress the thermosensitivity of green fluorescent protein. Curr Biol 1996;6:1653-1663.
-
(1996)
Curr Biol
, vol.6
, pp. 1653-1663
-
-
Siemering, K.R.1
Golbik, R.2
Sever, R.3
Haseloff, J.4
-
26
-
-
0028894696
-
Red-shifted excitation mutants of the green fluorescent protein
-
Delagrave S, Hawtin RE, Silva CM, Yang MM, Youvan DC: Red-shifted excitation mutants of the green fluorescent protein. Biotechnology (NY) 1995;13:151-154.
-
(1995)
Biotechnology (NY)
, vol.13
, pp. 151-154
-
-
Delagrave, S.1
Hawtin, R.E.2
Silva, C.M.3
Yang, M.M.4
Youvan, D.C.5
-
27
-
-
0029973636
-
FACS-optimized mutants of the green fluorescent protein (GFP)
-
Cormack BP, Valdivia RH, Falkow S: FACS-optimized mutants of the green fluorescent protein (GFP). Gene 1996;173:33-38.
-
(1996)
Gene
, vol.173
, pp. 33-38
-
-
Cormack, B.P.1
Valdivia, R.H.2
Falkow, S.3
-
28
-
-
0029670330
-
Improved green fluorescent protein by molecular evolution using DNA shuffling
-
Crameri A, Whitehorn EA, Tate E, Stemmer WP: Improved green fluorescent protein by molecular evolution using DNA shuffling. Nat Biotechnol 1996;14:315-319
-
(1996)
Nat Biotechnol
, vol.14
, pp. 315-319
-
-
Crameri, A.1
Whitehorn, E.A.2
Tate, E.3
Stemmer, W.P.4
-
29
-
-
0034601826
-
Folding of green fluorescent protein, the cycle3 mutant
-
Fukuda H, Arai M, Kuwajima K: Folding of green fluorescent protein and the cycle3 mutant. Biochemistry 2000;39:12025-12032.
-
(2000)
Biochemistry
, vol.39
, pp. 12025-12032
-
-
Fukuda, H.1
Arai, M.2
Kuwajima, K.3
-
30
-
-
0030087710
-
Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer
-
Heim R, Tsien RY: Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr Biol 1996;6:178-182.
-
(1996)
Curr Biol
, vol.6
, pp. 178-182
-
-
Heim, R.1
Tsien, R.Y.2
-
31
-
-
0031555862
-
A novel mutation which enhances the fluorescence of green fluorescent protein at high temperatures
-
Kimata Y, Iwaki M, Lim CR, Kohno K: A novel mutation which enhances the fluorescence of green fluorescent protein at high temperatures. Biochem Biophys Res Commun 1997;232:69-73.
-
(1997)
Biochem Biophys Res Commun
, vol.232
, pp. 69-73
-
-
Kimata, Y.1
Iwaki, M.2
Lim, C.R.3
Kohno, K.4
-
32
-
-
0037012847
-
Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells
-
Zacharias DA, Violin JD, Newton AC, Tsien RY: Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science 2002;296:913-916.
-
(2002)
Science
, vol.296
, pp. 913-916
-
-
Zacharias, D.A.1
Violin, J.D.2
Newton, A.C.3
Tsien, R.Y.4
-
33
-
-
0029061274
-
Green-fluorescent protein mutants with altered fluorescence excitation spectra
-
Ehrig T, O'Kane DJ, Prendergast FG: Green-fluorescent protein mutants with altered fluorescence excitation spectra. FEBS Lett 1995;367:163-166.
-
(1995)
FEBS Lett
, vol.367
, pp. 163-166
-
-
Ehrig, T.1
O'Kane, D.J.2
Prendergast, F.G.3
-
34
-
-
0030832226
-
On/off blinking and switching behaviour of single molecules of green fluorescent protein
-
Dickson RM, Cubitt AB, Tsien RY, Moerner WE: On/off blinking and switching behaviour of single molecules of green fluorescent protein. Nature 1997;388:355-358.
-
(1997)
Nature
, vol.388
, pp. 355-358
-
-
Dickson, R.M.1
Cubitt, A.B.2
Tsien, R.Y.3
Moerner, W.E.4
-
35
-
-
0032499784
-
Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins
-
Llopis J, McCaffery JM, Miyawaki A, Farquhar MG, Tsien RY: Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins. Proc Natl Acad Sci USA 1998;95:6803-6808.
-
(1998)
Proc Natl Acad Sci USA
, vol.95
, pp. 6803-6808
-
-
Llopis, J.1
McCaffery, J.M.2
Miyawaki, A.3
Farquhar, M.G.4
Tsien, R.Y.5
-
36
-
-
0035800773
-
Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism applications
-
Griesbeck O, Baird GS, Campbell RE, Zacharias DA, Tsien RY: Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications. J Biol Chem 2001;276:29188-194.
-
(2001)
J Biol Chem
, vol.276
, pp. 29188-29194
-
-
Griesbeck, O.1
Baird, G.S.2
Campbell, R.E.3
Zacharias, D.A.4
Tsien, R.Y.5
-
37
-
-
0036138908
-
A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications
-
Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A: A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol 2002;20:87-90.
-
(2002)
Nat Biotechnol
, vol.20
, pp. 87-90
-
-
Nagai, T.1
Ibata, K.2
Park, E.S.3
Kubota, M.4
Mikoshiba, K.5
Miyawaki, A.6
-
38
-
-
0032404444
-
Aequorea victoria bioluminescence moves into an exciting new era
-
Kendall JM, Badminton MN: Aequorea victoria bioluminescence moves into an exciting new era. Trends Biotechnol 1998;16:216-224.
-
(1998)
Trends Biotechnol
, vol.16
, pp. 216-224
-
-
Kendall, J.M.1
Badminton, M.N.2
-
40
-
-
0032867477
-
Fluorescent proteins from nonbioluminescent Anthozoa species
-
Matz MV, Fradkov AF, Labas YA, Savitsky AP, Zaraisky AG, Markelov ML, Lukyanov SA: Fluorescent proteins from nonbioluminescent Anthozoa species. Nat Biotechnol 1999;17:969-973.
-
(1999)
Nat Biotechnol
, vol.17
, pp. 969-973
-
-
Matz, M.V.1
Fradkov, A.F.2
Labas, Y.A.3
Savitsky, A.P.4
Zaraisky, A.G.5
Markelov, M.L.6
Lukyanov, S.A.7
-
41
-
-
0035913959
-
GFP-like chromoproteins as a source of far-red fluorescent proteins
-
Gurskaya NG, Fradkov AF, Terskikh A, Matz MV, Labas YA, Martynov VI, Yanushevich YG, Lukyanov KA, Lukyanov SA: GFP-like chromoproteins as a source of far-red fluorescent proteins. FEBS Lett 2001;507:16-20.
-
(2001)
FEBS Lett
, vol.507
, pp. 16-20
-
-
Gurskaya, N.G.1
Fradkov, A.F.2
Terskikh, A.3
Matz, M.V.4
Labas, Y.A.5
Martynov, V.I.6
Yanushevich, Y.G.7
Lukyanov, K.A.8
Lukyanov, S.A.9
-
42
-
-
0037007086
-
Diversity, evolution of the green fluorescent protein family
-
Labas YA, Gurskaya NG, Yanushevich YG, Fradkov AF, Lukyanov KA, Lukyanov SA, Matz MV: Diversity and evolution of the green fluorescent protein family. Proc Natl Acad Sci USA 2002;99:4256-4261.
-
(2002)
Proc Natl Acad Sci USA
, vol.99
, pp. 4256-4261
-
-
Labas, Y.A.1
Gurskaya, N.G.2
Yanushevich, Y.G.3
Fradkov, A.F.4
Lukyanov, K.A.5
Lukyanov, S.A.6
Matz, M.V.7
-
43
-
-
0037062424
-
A monomeric red fluorescent protein
-
Campbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharias DA, Tsien RY: A monomeric red fluorescent protein. Proc Natl Acad Sci USA 2002;99:7877-7882.
-
(2002)
Proc Natl Acad Sci USA
, vol.99
, pp. 7877-7882
-
-
Campbell, R.E.1
Tour, O.2
Palmer, A.E.3
Steinbach, P.A.4
Baird, G.S.5
Zacharias, D.A.6
Tsien, R.Y.7
-
44
-
-
0030736417
-
A beta-arrestin/green fluorescent protein biosensor for detecting G protein-coupled receptor activation
-
Barak L, Ferguson SS, Zhang J, Caron MG: A beta-arrestin/green fluorescent protein biosensor for detecting G protein-coupled receptor activation. J Biol Chem 1997;272:27497-500.
-
(1997)
J Biol Chem
, vol.272
, pp. 27497-27500
-
-
Barak, L.1
Ferguson, S.S.2
Zhang, J.3
Caron, M.G.4
-
45
-
-
0027200260
-
Mechanisms of multifunctional signalling by G protein-linked receptors
-
Milligan G: Mechanisms of multifunctional signalling by G protein-linked receptors. Trends Pharmacol Sci 1993;14:239-244.
-
(1993)
Trends Pharmacol Sci
, vol.14
, pp. 239-244
-
-
Milligan, G.1
-
46
-
-
0037013265
-
Oligomerization of G-protein-coupled receptors shown by selective co-immunoprecipitation
-
Salim K, Fenton T, Bacha J, Urien-Rodriguez H, Bonnert T Skynner HA, Watts E, Kerby J, Heald A, Beer M, McAllister G, Guest PC: Oligomerization of G-protein-coupled receptors shown by selective co-immunoprecipitation. J Biol Chem 2002;277:15482-15485.
-
(2002)
J Biol Chem
, vol.277
, pp. 15482-15485
-
-
Salim, K.1
Fenton, T.2
Bacha, J.3
Urien-Rodriguez, H.4
Bonnert, T.5
Skynner, H.A.6
Watts, E.7
Kerby, J.8
Heald, A.9
Beer, M.10
McAllister, G.11
Guest, P.C.12
-
47
-
-
0034724192
-
Detection of beta 2-adrenergic receptor dimerization in living cells using bioluminescence resonance energy transfer (BRET)
-
Angers S, Salahpour A, Joly E, Hilairet S, Chelsky D, Dennis M, Bouvier M: Detection of beta 2-adrenergic receptor dimerization in living cells using bioluminescence resonance energy transfer (BRET). Proc Natl Acad Sci USA 2000;97:3684-3689.
-
(2000)
Proc Natl Acad Sci USA
, vol.97
, pp. 3684-3689
-
-
Angers, S.1
Salahpour, A.2
Joly, E.3
Hilairet, S.4
Chelsky, D.5
Dennis, M.6
Bouvier, M.7
-
48
-
-
0035937571
-
Receptor-mediated activation of heterotrimeric G-proteins in living cells
-
Janetopoulos C, Jin T, Devreotes P: Receptor-mediated activation of heterotrimeric G-proteins in living cells. Science 2001;291:2408-2411.
-
(2001)
Science
, vol.291
, pp. 2408-2411
-
-
Janetopoulos, C.1
Jin, T.2
Devreotes, P.3
-
49
-
-
0035893654
-
Functional expression, FRET analysis of green fluorescent proteins fused to G-protein subunits in rat sympathetic neurons
-
Ruiz-Velasco V, Ikeda SR: Functional expression and FRET analysis of green fluorescent proteins fused to G-protein subunits in rat sympathetic neurons. J Physiol 2001;537:679-692.
-
(2001)
J Physiol
, vol.537
, pp. 679-692
-
-
Ruiz-Velasco, V.1
Ikeda, S.R.2
-
50
-
-
0033612277
-
Unlocking family secrets, K+ channel transmembrane domains
-
Clapham DE: Unlocking family secrets: K+ channel transmembrane domains. Cell 1999;97:547-550.
-
(1999)
Cell
, vol.97
, pp. 547-550
-
-
Clapham, D.E.1
-
51
-
-
0344222200
-
HTS approaches to voltage-gated ion channel drug discovery
-
Denyer J, Worley J, Cox B, Allenby G, Banks M: HTS approaches to voltage-gated ion channel drug discovery. Drug Discovery Today 1998;3:323-332.
-
(1998)
Drug Discovery Today
, vol.3
, pp. 323-332
-
-
Denyer, J.1
Worley, J.2
Cox, B.3
Allenby, G.4
Banks, M.5
-
52
-
-
0344500746
-
Cell-based assays and instrumentation for screening ion-channel targets
-
Gonzalez JE, Oades K, Leychkis Y, Harootunian A, Negulescu PA: Cell-based assays and instrumentation for screening ion-channel targets. Drug Discov Today 1999;4:431-439.
-
(1999)
Drug Discov Today
, vol.4
, pp. 431-439
-
-
Gonzalez, J.E.1
Oades, K.2
Leychkis, Y.3
Harootunian, A.4
Negulescu, P.A.5
-
53
-
-
0030834492
-
A genetically encoded optical probe of membrane voltage
-
Siegel MS, Isacoff EY: A genetically encoded optical probe of membrane voltage. Neuron 1997;19:735-741.
-
(1997)
Neuron
, vol.19
, pp. 735-741
-
-
Siegel, M.S.1
Isacoff, E.Y.2
-
54
-
-
0036221893
-
A genetically targetable fluorescent probe of channel gating with rapid kinetics
-
Ataka K, Pieribone VA. A genetically targetable fluorescent probe of channel gating with rapid kinetics. Biophys J 2002;82:509-516.
-
(2002)
Biophys J
, vol.82
, pp. 509-516
-
-
Ataka, K.1
Pieribone, V.A.2
-
55
-
-
0027397544
-
Inositol trisphosphate, calcium signalling
-
Berridge MJ: Inositol trisphosphate and calcium signalling. Nature 1993;361:315-325.
-
(1993)
Nature
, vol.361
, pp. 315-325
-
-
Berridge, M.J.1
-
56
-
-
0028831997
-
Calcium signaling
-
Clapham DE: Calcium signaling. Cell 1995;80:259-268.
-
(1995)
Cell
, vol.80
, pp. 259-268
-
-
Clapham, D.E.1
-
57
-
-
0031758718
-
Fluorescence techniques for measuring ion channel activity
-
Velicelebi G, Stauderman KA, Varney MA, Akong M, Hess SD, Johnson EC: Fluorescence techniques for measuring ion channel activity. Methods Enzymol 1999;294:20-47.
-
(1999)
Methods Enzymol
, vol.294
, pp. 20-47
-
-
Velicelebi, G.1
Stauderman, K.A.2
Varney, M.A.3
Akong, M.4
Hess, S.D.5
Johnson, E.C.6
-
58
-
-
0024488636
-
Fluorescent probes of cell signaling
-
Tsien RY: Fluorescent probes of cell signaling. Annu Rev Neurosci 1989;12:227-253.
-
(1989)
Annu Rev Neurosci
, vol.12
, pp. 227-253
-
-
Tsien, R.Y.1
-
60
-
-
0031011418
-
2+-dependent changes in the fluorescence emission of an indicator composed of two green fluorescent protein variants linked by a calmodulin-binding sequence. A new class of fluorescent indicators
-
2+-dependent changes in the fluorescence emission of an indicator composed of two green fluorescent protein variants linked by a calmodulin-binding sequence. A new class of fluorescent indicators. J Biol Chem 1997;272:13270-13274.
-
(1997)
J Biol Chem
, vol.272
, pp. 13270-13274
-
-
Romoser, V.A.1
Hinkle, P.M.2
Persechini, A.3
-
63
-
-
0029941366
-
Deletion mapping of the Aequorea victoria green fluorescent protein
-
Dopf J, Horiagon TM: Deletion mapping of the Aequorea victoria green fluorescent protein. Gene 1996;173:39-44.
-
(1996)
Gene
, vol.173
, pp. 39-44
-
-
Dopf, J.1
Horiagon, T.M.2
-
64
-
-
0032788447
-
Circularly permuted variants of the green fluorescent protein
-
Topell S, Hennecke J, Glockshuber R: Circularly permuted variants of the green fluorescent protein. FEBS Lett 1999;457:283-289.
-
(1999)
FEBS Lett
, vol.457
, pp. 283-289
-
-
Topell, S.1
Hennecke, J.2
Glockshuber, R.3
-
65
-
-
0034019496
-
A fluorescent indicator for visualizing cAMP-induced phosphorylation in vivo
-
Nagai Y, Miyazaki M, Aoki R, Zama T, Inouye S, Hirose K, Iino M, Hagiwara M: A fluorescent indicator for visualizing cAMP-induced phosphorylation in vivo. Nat Biotechnol 2000;18:313-316.
-
(2000)
Nat Biotechnol
, vol.18
, pp. 313-316
-
-
Nagai, Y.1
Miyazaki, M.2
Aoki, R.3
Zama, T.4
Inouye, S.5
Hirose, K.6
Iino, M.7
Hagiwara, M.8
-
66
-
-
0035129282
-
A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein
-
Nakai J, Ohkura M, Imoto K: A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein. Nat Biotechnol 2001;19:137-141.
-
(2001)
Nat Biotechnol
, vol.19
, pp. 137-141
-
-
Nakai, J.1
Ohkura, M.2
Imoto, K.3
-
68
-
-
0024423668
-
Identification of the cystic fibrosis gene, genetic analysis
-
Kerem B, Rommens JM, Buchanan JA, Markiewicz D, Cox TK, Chakravarti A, Buchwald M, Tsui LC: Identification of the cystic fibrosis gene: genetic analysis. Science 1989;245:1073-1080.
-
(1989)
Science
, vol.245
, pp. 1073-1080
-
-
Kerem, B.1
Rommens, J.M.2
Buchanan, J.A.3
Markiewicz, D.4
Cox, T.K.5
Chakravarti, A.6
Buchwald, M.7
Tsui, L.C.8
-
69
-
-
0026705098
-
The skeletal muscle chloride channel in dominant, recessive human myotonia
-
Koch MC, Steinmeyer K, Lorenz C, Ricker K, Wolf F, Otto M, Zoll B, Lehmann-Horn F, Grzeschik KH, Jentsch TJ: The skeletal muscle chloride channel in dominant and recessive human myotonia. Science 1992;257:797-800.
-
(1992)
Science
, vol.257
, pp. 797-800
-
-
Koch, M.C.1
Steinmeyer, K.2
Lorenz, C.3
Ricker, K.4
Wolf, F.5
Otto, M.6
Zoll, B.7
Lehmann-Horn, F.8
Grzeschik, K.H.9
Jentsch, T.J.10
-
70
-
-
0033724998
-
A genetically encoded ratiometric indicator for chloride capturing chloride transients in cultured hippocampal neurons
-
Kuner T, Augustine GJ: A genetically encoded ratiometric indicator for chloride: capturing chloride transients in cultured hippocampal neurons. Neuron. 2000;27:447-459.
-
(2000)
Neuron
, vol.27
, pp. 447-459
-
-
Kuner, T.1
Augustine, G.J.2
-
71
-
-
0023841862
-
A view of acidic intracellular compartments
-
Anderson RG, Orci L: A view of acidic intracellular compartments. J Cell Biol 1988;106(3):539-543.
-
(1988)
J Cell Biol
, vol.106
, Issue.3
, pp. 539-543
-
-
Anderson, R.G.1
Orci, L.2
-
72
-
-
0032500053
-
Visualizing secreton, synaptic transmission with pH-sensitive green fluorescent proteins
-
Miesenbock G, De Angelis DA, Rothman JE: Visualizing secreton and synaptic transmission with pH-sensitive green fluorescent proteins. Nature 1998;394:192-195.
-
(1998)
Nature
, vol.394
, pp. 192-195
-
-
Miesenbock, G.1
De Angelis, D.A.2
Rothman, J.E.3
-
73
-
-
0027404263
-
Beta-adrenergic receptor sequestration. A potential mechanism of receptor resensitization
-
Yu SS, Lefkowitz RJ, Hausdorff WP: Beta-adrenergic receptor sequestration. A potential mechanism of receptor resensitization. J Biol Chem 1993;268:337-341.
-
(1993)
J Biol Chem
, vol.268
, pp. 337-341
-
-
Yu, S.S.1
Lefkowitz, R.J.2
Hausdorff, W.P.3
-
74
-
-
0025343230
-
Signal transduction by receptors with tyrosine kinase activity
-
Ullrich A, Schlessinger J: Signal transduction by receptors with tyrosine kinase activity. Cell 1990;61:203-212.
-
(1990)
Cell
, vol.61
, pp. 203-212
-
-
Ullrich, A.1
Schlessinger, J.2
-
76
-
-
0034614490
-
Signaling-2000 and beyond
-
Hunter T: Signaling-2000 and beyond. Cell 2000;100:113-127.
-
(2000)
Cell
, vol.100
, pp. 113-127
-
-
Hunter, T.1
-
78
-
-
0035956940
-
Spatiotemporal dynamics of guanosine 3′,5′-cyclic monophosphate revealed by a genetically encoded, fluorescent indicator
-
Honda A, Adams SR, Sawyer CL, Lev-Ram V, Tsien RY, Dostmann WR: Spatiotemporal dynamics of guanosine 3′,5′-cyclic monophosphate revealed by a genetically encoded, fluorescent indicator. Proc Natl Acad Sci USA 2001;98:2437-2442.
-
(2001)
Proc Natl Acad Sci USA
, vol.98
, pp. 2437-2442
-
-
Honda, A.1
Adams, S.R.2
Sawyer, C.L.3
Lev-Ram, V.4
Tsien, R.Y.5
Dostmann, W.R.6
-
79
-
-
0035909994
-
Genetically encoded fluorescent reporters of protein tyrosine kinase activities in living cells
-
Ting AY, Kain KH, Klemke RL, Tsien RY: Genetically encoded fluorescent reporters of protein tyrosine kinase activities in living cells. Proc Natl Acad Sci USA 2001;98:15003-15008.
-
(2001)
Proc Natl Acad Sci USA
, vol.98
, pp. 15003-15008
-
-
Ting, A.Y.1
Kain, K.H.2
Klemke, R.L.3
Tsien, R.Y.4
-
80
-
-
0036196296
-
Fluorescent indicators for imaging protein phosphorylation in single living cells
-
Sato M, Ozawa T, Inukai K, Asano T, Umezawa Y: Fluorescent indicators for imaging protein phosphorylation in single living cells. Nat Biotechnol 2002;20:287-294.
-
(2002)
Nat Biotechnol
, vol.20
, pp. 287-294
-
-
Sato, M.1
Ozawa, T.2
Inukai, K.3
Asano, T.4
Umezawa, Y.5
-
81
-
-
0034284637
-
Mitochondria as the central control point of apoptosis
-
Desagher S, Martinou JC: Mitochondria as the central control point of apoptosis. Trends Cell Biol 2000;10:369-377.
-
(2000)
Trends Cell Biol
, vol.10
, pp. 369-377
-
-
Desagher, S.1
Martinou, J.C.2
-
82
-
-
0033781027
-
The coordinate release of cytochrome c during apoptosis is rapid, complete and kinetically invariant
-
Goldstein JC, Waterhouse NJ, Juin P, Evan GI, Green DR: The coordinate release of cytochrome c during apoptosis is rapid, complete and kinetically invariant. Nat Cell Biol 2000;2:156-162.
-
(2000)
Nat Cell Biol
, vol.2
, pp. 156-162
-
-
Goldstein, J.C.1
Waterhouse, N.J.2
Juin, P.3
Evan, G.I.4
Green, D.R.5
-
83
-
-
0031865351
-
Bcl-2 and Bax interactions in mitochondria probed with green fluorescent protein, fluorescence resonance energy transfer
-
Mahajan NP, Linder K, Berry G, Gordon GW, Heim R, Herman B: Bcl-2 and Bax interactions in mitochondria probed with green fluorescent protein and fluorescence resonance energy transfer. Nat Biotechnol 1998;16:547-552.
-
(1998)
Nat Biotechnol
, vol.16
, pp. 547-552
-
-
Mahajan, N.P.1
Linder, K.2
Berry, G.3
Gordon, G.W.4
Heim, R.5
Herman, B.6
-
84
-
-
0032522223
-
Detection of programmed cell death using fluorescence energy transfer
-
Xu X, Gerard AL, Huang BC, Anderson DC, Payan DG, Luo Y. Detection of programmed cell death using fluorescence energy transfer. Nucleic Acids Res 1998;26:2034-2035.
-
(1998)
Nucleic Acids Res
, vol.26
, pp. 2034-2035
-
-
Xu, X.1
Gerard, A.L.2
Huang, B.C.3
Anderson, D.C.4
Payan, D.G.5
Luo, Y.6
-
85
-
-
0033777736
-
Development application of a GFP-FRET intracellular caspase assay for drug screening
-
Jones J, Heim R, Hare E, Stack J, Pollok BA. Development and application of a GFP-FRET intracellular caspase assay for drug screening. J Biomol Screen 2000;5:307-318.
-
(2000)
J Biomol Screen
, vol.5
, pp. 307-318
-
-
Jones, J.1
Heim, R.2
Hare, E.3
Stack, J.4
Pollok, B.A.5
-
86
-
-
0034801529
-
Application of the fluorescence resonance energy transfer method for studying the dynamics of caspase-3 activation during UV-induced apoptosis in living HeLa cells
-
Luo KQ, Yu VC, Pu Y, Chang DC: Application of the fluorescence resonance energy transfer method for studying the dynamics of caspase-3 activation during UV-induced apoptosis in living HeLa cells. Biochem Biophys Res Commun 2001;283:1054-1060.
-
(2001)
Biochem Biophys Res Commun
, vol.283
, pp. 1054-1060
-
-
Luo, K.Q.1
Yu, V.C.2
Pu, Y.3
Chang, D.C.4
-
87
-
-
0036199586
-
A perspective on protein microarrays
-
Mitchell P: A perspective on protein microarrays. Nat Biotechnol 2002;20:225-229.
-
(2002)
Nat Biotechnol
, vol.20
, pp. 225-229
-
-
Mitchell, P.1
-
88
-
-
0033491334
-
Motif trap, a rapid method to clone motifs that can target proteins to defined subcellular localizations
-
Bejarano LA, Gonzalez C: Motif trap: a rapid method to clone motifs that can target proteins to defined subcellular localizations. J Cell Sci 1999;112:4207-4211.
-
(1999)
J Cell Sci
, vol.112
, pp. 4207-4211
-
-
Bejarano, L.A.1
Gonzalez, C.2
-
89
-
-
0033549555
-
A visual screen of a GFP-fusion library identifies a new type of nuclear envelope membrane protein
-
Rolls MM, Stein PA, Taylor SS, Ha E, McKeon F, Rapoport TA: A visual screen of a GFP-fusion library identifies a new type of nuclear envelope membrane protein. J Cell Biol 1999;146:29-44.
-
(1999)
J Cell Biol
, vol.146
, pp. 29-44
-
-
Rolls, M.M.1
Stein, P.A.2
Taylor, S.S.3
Ha, E.4
McKeon, F.5
Rapoport, T.A.6
-
90
-
-
0034177506
-
Protein traps, using intracellular localization for cloning
-
Gonzalez C, Bejarano LA: Protein traps: using intracellular localization for cloning. Trends Cell Biol 2000;10:162-165.
-
(2000)
Trends Cell Biol
, vol.10
, pp. 162-165
-
-
Gonzalez, C.1
Bejarano, L.A.2
-
91
-
-
0034280113
-
Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing
-
Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S: Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing. EMBO Reports 2000;1:287-292.
-
(2000)
EMBO Reports
, vol.1
, pp. 287-292
-
-
Simpson, J.C.1
Wellenreuther, R.2
Poustka, A.3
Pepperkok, R.4
Wiemann, S.5
-
92
-
-
0034714469
-
A visual intracellular classification strategy for uncharacterized human proteins
-
Hoja MR, Wahlestedt C, Hoog C: A visual intracellular classification strategy for uncharacterized human proteins. Exp Cell Res 2000;259(1):239-246.
-
(2000)
Exp Cell Res
, vol.259
, Issue.1
, pp. 239-246
-
-
Hoja, M.R.1
Wahlestedt, C.2
Hoog, C.3
-
93
-
-
0037045398
-
ORF-FINDER: A vector for high-throughput gene identification
-
Rombel IT, Sykes KF, Rayner S, Johnston SA: ORF-FINDER: a vector for high-throughput gene identification. Gene 2002;282:33-41.
-
(2002)
Gene
, vol.282
, pp. 33-41
-
-
Rombel, I.T.1
Sykes, K.F.2
Rayner, S.3
Johnston, S.A.4
-
94
-
-
0034883501
-
Using green fluorescent protein to study intracellular signalling
-
Tavare JM, Fletcher LM, Welsh GI: Using green fluorescent protein to study intracellular signalling. J Endocrinol 2001;170:297-306.
-
(2001)
J Endocrinol
, vol.170
, pp. 297-306
-
-
Tavare, J.M.1
Fletcher, L.M.2
Welsh, G.I.3
-
95
-
-
0033324780
-
Visualizing protein-protein interactions in the nucleus of the living cell
-
Day RN, Nordeen SK, Wan Y: Visualizing protein-protein interactions in the nucleus of the living cell. Mol Endocrinol 1999;13:517-526.
-
(1999)
Mol Endocrinol
, vol.13
, pp. 517-526
-
-
Day, R.N.1
Nordeen, S.K.2
Wan, Y.3
-
96
-
-
0033083490
-
Using GFP in FRET-based applications
-
Pollok BA, Heim R: Using GFP in FRET-based applications. Trends Cell Biol 1999;9:57-60.
-
(1999)
Trends Cell Biol
, vol.9
, pp. 57-60
-
-
Pollok, B.A.1
Heim, R.2
-
97
-
-
0030738524
-
Monitoring protein-protein interactions in intact eukaryotic cells by beta-galactosidase complementation
-
Rossi F, Charlton CA, Blau HM: Monitoring protein-protein interactions in intact eukaryotic cells by beta-galactosidase complementation. Proc Natl Acad Sci USA 1997;94:8405-8410.
-
(1997)
Proc Natl Acad Sci USA
, vol.94
, pp. 8405-8410
-
-
Rossi, F.1
Charlton, C.A.2
Blau, H.M.3
-
98
-
-
0032977886
-
An in vivo library-versus-library selection of optimized protein-protein interactions
-
Pelletier JN, Arndt KM, Pluckthun A, Michnick SW: An in vivo library-versus-library selection of optimized protein-protein interactions. Nat Biotechnol 1999;17:683-690.
-
(1999)
Nat Biotechnol
, vol.17
, pp. 683-690
-
-
Pelletier, J.N.1
Arndt, K.M.2
Pluckthun, A.3
Michnick, S.W.4
-
99
-
-
0035894271
-
Protein splicing-based reconstitution of split green fluorescent protein for monitoring protein-protein interactions in bacteria: Improved sensitivity and reduced screening time
-
Ozawa T, Takeuchi TM, Kaihara A, Sato M, Umezawa Y:Protein splicing-based reconstitution of split green fluorescent protein for monitoring protein-protein interactions in bacteria: improved sensitivity and reduced screening time. Anal Chem 2001;73:5866-5874.
-
(2001)
Anal Chem
, vol.73
, pp. 5866-5874
-
-
Ozawa, T.1
Takeuchi, T.M.2
Kaihara, A.3
Sato, M.4
Umezawa, Y.5
-
100
-
-
0034329620
-
A fluorescent indicator for detecting protein-protein interactions in vivo based on protein splicing
-
Ozawa T, Nogami S, Sato M, Ohya Y, Umezawa Y: A fluorescent indicator for detecting protein-protein interactions in vivo based on protein splicing. Anal Chem 2000;72:5151-5157.
-
(2000)
Anal Chem
, vol.72
, pp. 5151-5157
-
-
Ozawa, T.1
Nogami, S.2
Sato, M.3
Ohya, Y.4
Umezawa, Y.5
-
101
-
-
0036241055
-
Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation and Rel family proteins in living cells using bimolecular fluorescence complementation
-
Hu CD, Chinenov Y, Kerppola TK. Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. Mol Cell 2002;9:789-798.
-
(2002)
Mol Cell
, vol.9
, pp. 789-798
-
-
Hu, C.D.1
Chinenov, Y.2
Kerppola, T.K.3
|