Development and application of a GFP-FRET intracellular caspase assay for drug screening

Journal of Biomolecular Screening

Volumn 5, Issue 5, 2000, Pages 307-317

  Jones, Jay ^a   Heim, Roger ^a   Hare, Eric ^a   Stack, Jeffrey ^a   Pollok, Brian A ^a  

^a Aurora Biosciences Corporation   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETAZOLAMIDE; ANTINEOPLASTIC AGENT; CAMPTOTHECIN; CASPASE 3; COMPLEMENTARY DNA; CYTARABINE; EMETINE; ETOPOSIDE; FAS ANTIGEN; GREEN FLUORESCENT PROTEIN; IMMUNOGLOBULIN M; IMMUNOGLOBULIN M ANTIBODY; STAUROSPORINE; THIOMERSAL;

APOPTOSIS; ARTICLE; CELL DEATH; CONTROLLED STUDY; CYTOLYSIS; DRUG SCREENING; ENERGY TRANSFER; ENZYME ACTIVATION; ENZYME ASSAY; FLUORESCENCE; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; TUMOR CELL;

AMINO ACID SEQUENCE; ANTIBODIES; ANTIGENS, CD95; APOPTOSIS; BIOSENSING TECHNIQUES; CASPASE 3; CASPASES; CYSTEINE PROTEINASE INHIBITORS; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG EVALUATION, PRECLINICAL; ENERGY TRANSFER; ENZYME ACTIVATION; FLOW CYTOMETRY; FLUORESCENCE; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; JURKAT CELLS; LUMINESCENT PROTEINS; MOLECULAR SEQUENCE DATA; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT FUSION PROTEINS; REPRODUCIBILITY OF RESULTS; TRANSFECTION;

EID: 0033777736     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1177/108705710000500502     Document Type: Article

References (25)

1. Apoptotic pathways: The roads to ruin
2. The green fluorescent protein
3. Using GFP in FRET-based applications
4. Detection of programmed cell death using fluorescence energy transfer
5. Novel mutant green fluorescent protein protease substrates reveal the activation of specific caspases during apoptosis
6. Caspases: Enemies within
7. All's well that ends dead
8. Caspase activity sows the seeds of neuronal death
9. Granzyme release and caspase activation in activated human T-lymphocytes
10. 2+ indicators with greatly improved fluorescence properties
11. A novel assay for apoptosis: Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled annexin V
12. 2+ based on green fluorescent proteins and calmodulin
13. Green fluorescent protein forms for energy transfer
14. Substrate specificities of caspase family proteases
15. Substrate and inhibitor specificity of interleukin-1β-converting enzyme and related caspases
16. Involvement of an ICE-like protease in apoptotic cells
17. CD95 (CD95)/CD95L interactions required for programmed cell death after T cell activation
18. CPP-32/apopain is a key interleukin-1β-converting enzyme-like protease involved in CD95-mediated apoptosis
19. 1 aspartate-based α-((2,6-dichlorobenzoy-1βoxy) methyl ketones as potent time-dependent inhibitors of interleukin-1(-converting enzyme
20. Role of Ced-3/ICE-family proteases in staurosporine-induced programmed cell death
21. A simple parameter for use in evaluation and validation of high throughput screening assays
22. Trichothecene mycotoxins trigger a ribotoxic stress response that activates c-Jun N-terminal kinase and p38 mitogen-activated protein kinase and induces apoptosis
23. Effect of thimerosal and other sulfhydryl reagents on calcium permeability in thymus lymphocytes
24. The mechanism of ara-C-induced apoptosis of differentiating cerebellar granule neurons
25. Different subcellular distribution of caspase-3 and caspase-7 following CD95-induced apoptosis in mouse liver