Bcl-2 and bax interactions in mitochondria probed with green fluorescent protein and fluorescence resonance energy transfer

Nature Biotechnology

Volumn 16, Issue 6, 1998, Pages 547-552

  Mahajan, Nupam P ^a   Linder, Katrina ^a   Berry, Gail ^a   Gordon, Gerald W ^a   Heim, Roger ^a   Herman, Brian ^a  

^a UNIVERSITY OF NORTH CAROLINA   (United States)

Author keywords

Apoptosis; Microscopy; Protein interactions

Indexed keywords

GREEN FLUORESCENT PROTEIN; PROTEIN BAX; PROTEIN BCL 2;

ANIMAL CELL; ARTICLE; CELL STRAIN 3T3; CELL STRAIN BHK; CONTROLLED STUDY; ENDOTHELIUM CELL; FLUORESCENCE; MITOCHONDRION; MOLECULAR INTERACTION; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION;

3T3 CELLS; ANIMALS; BCL-2-ASSOCIATED X PROTEIN; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; INDICATORS AND REAGENTS; LUMINESCENT PROTEINS; MICE; MICROSCOPY, FLUORESCENCE; MITOCHONDRIA; MOLECULAR PROBES; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-BCL-2; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, FLUORESCENCE;

ANIMALIA; HUMAN PAPILLOMAVIRUS; MAMMALIA;

EID: 0031865351     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt0698-547     Document Type: Article

References (26)

1. Tsujimoto, Y. and Croce, C.M. 1986. Analysis of the structure, transcripts and protein products of bcl-2, the gene involved in human follicular lymphoma. Proc. Natl. Acad. Sci. USA 83:5214-5218.
2. Rampino, N., Yamamoto, H., lonov, Y., Li, Y, Sawai, H., Reed, U.C. and Perucho, M. 1997. Somatic frameshift mutations in the BAX gene in colon cancers of the microsatellite mutator phenotype. Science 275:967-969.
3. Yin, C., Knudson, M.C., Korsymeyer, S.J., and Dyke, T.V. 1997. Bax suppresses tumorigenesis and stimulates apoptosis in vivo. Nature 385:637-640.
4. Hockenbery, D.M., Nunez, G., Milliman, C., Schreiber, R.D., and Korsmeyer, S. J. 1990. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 348:334-336.
5. Oltavi, Z.N., Milliman, C.L., and Korsmeyer, S.J. 1993. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 74:609-619.
6. Oltavi, Z.N. and Korsmeyer, S.J. 1994. Checkpoints of dueling dimers foil death wishes Cell 79:189-192.
7. Xiang, J., Chao, D.T., and Korsmeyer, S.J. 1996. BAX-induced cell death may not require interleukin 1 B-converting enzyme-like proteases. Proc. Natl. Acad. Sci. USA 93:14599-14563.
8. Liu, X., Kim, C.N.,Yang, J., Jemmerson, R., and Wang, X. 1996. Induction of apoptotic program in cell-free extracts: requirement of dATP and cytochrome c. Cell 83:147-157.
9. Chinnaiyan, A.M., O'Rouke, K., Lane, B.R., and Dixit, V.M. 1997. Interaction of CED-4 with CED-3 and CED-9: A molecular framework for cell death Science 275:1122-1126.
10. Wu, D., Wallen, H. D., and Nunez, G. 1997. Interaction and regulation of subcellular localization of CED-4 by CED-9 Science 275:1126-1129.
11. Yang, J., Liu, X., Bhalla, K., Kim, C.N., Ibrado, A.M., Cai, J. et al. 1997. Prevention of apoptosis by Bcl-2: Release of cytochrome c from mitochondria blocked Science 275:1129-1132.
12. Knudson M.C. and Korsmeyer S.J. 1997. Bcl-2 and Bax functions independently to regulate cell death Nat. Genet. 16,358-363.
13. Reed J.C., Zha H., Aime-Sempe, C., Takayama S. and Wang, H. 1996. Structurefunction analysis of Bcl-2 family proteins, pp 99-112 in Mechanisms of lymphocyte activation and immune regulation VI. Gupta and Cohen (eds.). Plenum Press, New York.
14. Cubitt, A.B., Heim, R., Adams, S.R., Boyd, A.E., Gross, L.A., and Tsien, R.Y. 1995. Understanding, improving and using green fluorescent proteins Trends in Biochemical Sciences 20:448-455.
15. Rizzuto, R., Brini, M., De Giorgi, F., Rossi, R., Heim, R., Tsien, R.Y, and Pozzan, T. 1996. Double labeling of subcellular structures with organelle-targeted GFP mutants in vivo. Curr. BibJ. 6:183-188.
16. Heim, R., Cubitt, A.B., and Tsien, R.Y. 1995. Improved green fluorescence. Nature 373:663-664.
17. Heim, R. and Tsien, R.Y. 1996. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer Curr. Biol. 6:178-182.
18. Mitra, R.D., Suva, C.M., and Youvan, D.C. 1996. Fluorescence resonance energy transfers between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein. Gene 173:13-17.
19. Miyawaki, A., Llopis, J., Heim, R., McCaffery, J.M., Adams, J.A., Ikura, M., and Tsien, R.Y. 1997. Fluorescence indicators for Ca2' based on green fluorescent proteins and calmodulin. Nature 388:882-887.
20. Gonsalez, D.H. and Neupert, W. 1990. Biogenesis of mitochondrial c-type cytochromes. J. Bioenerg. Biomembr. 22:753-768.
21. Hoppe-Seyler, F. and Butz, K. 1993. Repression of endogenous p53 transactivation function in Hela cervical carcinoma cells by human papillomavirus type 16 E6, human mdm-2 and mutant p53. J. Wot. 67:3111-3117.
22. Brown, J., Higo, H., McKalip, A., and Herman, B. 1997. Human papillomavirus (HPV) 16 E6 sensitizes cells to stractyloside-induced spoptosis: role of p53, ICE-like proteases and the mitochondrial permeability transition. J. Cell. Biochem. 66:245-255.
23. Casciola-Rosen, L.A., Nicholson, D.W., Chong, T., Rowan, K.R., Thornberry, N.A., Miller, O.K., and Rosen, A. 1996. Apopain/CPP32 cleaves proteins that are essential for cellular repair, a fundamental principle of apoptotic cell death. J. Exp. Med. 183:1957-1964.
24. Gordon, G.W., Berry, G., Liang, X.H., Levine, B., and Herman, B. 1998. Quantitative fluorescence resonance energy transfer (FRET) measurements using fluorescence microscopy. Biophysical Journal In press.
25. Terry, B.R., Matthews, E.K., and Haseloff, J. 1995. Molecular characterization of recombinant green fluorescent protein by fluorescence correlation microscopy. Biochem. Biophys. Res. Commun. 217,21-27.
26. Kharbanda, S., Pandey, P., Schofield, L, Israels, S., Roncinske, R., Yoshida, K. et al. 1997. Role of Bcl-X,. as an inhibitor of cytosolic cytochrome c accumulation in DNA damage-induced apoptosis. Proc. Natl. Acad. Sci. USA 94:6939-6942.