A visual screen of a GFP-fusion library identifies a new type of nuclear envelope membrane protein

Journal of Cell Biology

Volumn 146, Issue 1, 1999, Pages 29-43

  Rolls, Melissa M ^b   Stein, Pascal A ^b   Taylor, Stephen S ^a,c   Ha, Edward ^a   McKeon, Frank ^a   Rapoport, Tom A ^b  

^a HARVARD MEDICAL SCHOOL   (United States)

^b HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^c UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Green fluorescent protein; Nuclear envelope; Nurim; Protein targeting; Visual screen

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; NUCLEAR PROTEIN;

ANIMAL CELL; ARTICLE; CELL NUCLEUS MEMBRANE; CONTROLLED STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN LOCALIZATION; SCREENING;

AMINO ACID SEQUENCE; ANIMALS; BIOLOGICAL TRANSPORT; CELL LINE; CELL NUCLEUS; CLONING, MOLECULAR; ENDOPLASMIC RETICULUM; FLUORESCENCE; GENE LIBRARY; GREEN FLUORESCENT PROTEINS; HUMANS; LAMINS; LUMINESCENT PROTEINS; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEAR ENVELOPE; NUCLEAR PROTEINS; PROTEIN SORTING SIGNALS; RECOMBINANT FUSION PROTEINS; SOLUBILITY; SUBCELLULAR FRACTIONS;

ANIMALIA; MAMMALIA;

EID: 0033549555     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.146.1.29     Document Type: Article

References (47)

1. Altschul, S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller, and D.J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402.
2. Bailer, S.M., H.M. Eppenberger, G. Griffiths, and E.A. Nigg. 1991. Characterization of a 54-kD protein of the inner nuclear membrane: evidence for a cell cycle-dependent interaction with the nuclear lamina. J. Cell Biol. 114:389-400.
3. Bastos, R., N. Panté, and B. Burke. 1995. Nuclear pore complex proteins. Int. Rev. Cytol. 162B:257-302.
4. Bergmann, J.E., and S.J. Singer. 1983. Immunoelectron microscopic studies of the intracellular transport of the membrane glycoprotein (G) of vesicular stomatitis virus in infected Chinese hamster ovary cells. J. Cell Biol. 97:1777-1787.
5. Bione, S., E. Maestrini, S. Rivella, M. Mancini, S. Regis, G. Romeo, and D. Toniolo. 1994. Identification of a novel X-linked gene responsible for Emery-Dreifuss muscular dystrophy. Nat. Genet. 8:323-327.
6. Bonne, G., M. Raffaele Di Barletta, S. Varnous, H.-M. Bécane, E.-H. Hammouda, L. Merlini, F. Muntoni, C.R. Greenberg, F. Gary, J.-A. Urtizberea, et al. 1999. Mutations in the gene encoding lamin A/C cause autosomal dominant Emery-Dreifuss muscular dystrophy. Nat. Genet. 21:285-288.
7. Burns, N., B. Grimwade, P.B. Ross-Macdonald, E.-Y. Choi, K. Finberg, G.S. Roeder, and M. Snyder. 1994. Large-scale analysis of gene expression, protein localization, and gene disruption in Saccharomyces cerevisiae. Genes Dev. 8:1087-1105.
8. Chaparian, M.G., and D.R. Evans. 1988. Intracellular location of the multidomain protein CAD in mammalian cells. FASEB (Fed. Am. Soc. Exp. Biol.) J. 2:2982-2989.
9. Davis, L.I., and G. Blobel. 1986. Identification and characterization of a nuclear pore complex protein. Cell. 45:699-709.
10. Davis, L.I., and G. Blobel. 1987. Nuclear pore complex contains a family of glycoproteins that includes p62: glycosylation through a previously unidentified cellular pathway. Proc. Natl. Acad. Sci. USA. 84:7552-7556.
11. Ellenberg, J., E.D. Siggia, J.E. Moreira, C.L. Smith, J.F. Presley, H.J. Worman, and J. Lippincott-Schwartz. 1997. Nuclear membrane dynamics and reassembly in living cells: targeting of an inner nuclear membrane protein in interphase and mitosis. J. Cell Biol. 138:1193-1206.
12. Ellis, J.A., M. Craxton, J.A. Yates, and J. Kendrick-Jones. 1998. Aberrant intracellular targeting and cell cycle-dependent phosphorylation of emerin contribute to the Emery-Dreifuss muscular dystrophy phenotype. J. Cell Sci. 111:781-792.
13. Foisner, R., and L. Gerace. 1993. Integral membrane proteins of the nuclear envelope interact with lamins and chromosomes, and binding is modulated by mitotic phosphorylation. Cell. 73:1267-1279.
14. Fricker, M., M. Hollinshead, N. White, and D. Vaux. 1997. Interphase nuclei of many mammalian cell types contain deep, dynamic, tubular membrane-bound invaginations of the nuclear envelope. J. Cell Biol. 136: 531-544.
15. Furukawa, K., N. Panté, U. Aebi, and L. Gerace. 1995. Cloning of a cDNA for lamina-associated polypeptide 2 (LAP2) and identification of regions that specify targeting to the nuclear envelope. EMBO (Eur. Mol. Biol. Organ.) J. 14:1626-1636.
16. Furukawa, K., C.E. Fritze, and L. Gerace. 1998. The major nuclear envelope targeting domain of LAP2 coincides with its lamin binding region but is distinct from its chromatin interaction domain. J. Biol. Chem. 273: 4213-4219.
17. Gant, T.M., and K.L. Wilson. 1997. Nuclear assembly. Annu. Rev. Cell Dev. Biol. 13:699-695.
18. Gant, T.M., C.A. Harris, and K.L. Wilson. 1999. Roles of LAP2 proteins in nuclear assembly and DNA replication: truncated LAP2β proteins alter lamina assembly, envelope formation, nuclear size, and DNA replication efficiency in Xenopus laevis extracts. J. Cell Biol. 144:1083-1096.
19. Gerace, L., Y. Ottaviano, and C. Kondor-Koch. 1982. Identification of a major polypeptide of the nuclear pore complex. J. Cell Biol. 95:826-837.
20. Heald, R., M. McLoughlin, and F. McKeon. 1993. Human weel maintains mitotic timing by protecting the nucleus from cytoplasmically activated Cdc2 kinase. Cell. 74:463-474.
21. Kitamura, T., M. Onishi, S. Kinoshita, A. Shibuya, A. Miyajima, and G.P. Nolan. 1995. Efficient screening of retroviral cDNA expression libraries. Proc. Natl. Acad. Sci. USA. 92:9146-9150.
22. Kozak, M. 1991. Structural features in eukaryotic mRNAs that modulate the initiation of translation. J. Biol. Chem. 266:19867-19870.
23. Lustig, K.D., P.T. Stukenberg, T.J. McGarry, R.W. King, V.L. Cryns, P.E. Mead, L.I. Zon, J. Yuan, and M.W. Kirschner. 1997. Small pool expression screening: identification of genes involved in cell cycle control, apoptosis, and early development. Methods Enzymol. 283:83-99.
24. Mahajan, R., C. Delphin, T. Guan, L. Gerace, and F. Melcior. 1997. A small ubiquitin-related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein Ran BP2. Cell. 88:97-107.
25. Malviya, A., and P.J. Rogue. 1998. "Tell me where is calcium bred": clarifying the roles of nuclear calcium. Cell. 92:17-23.
26. Manilal, S., N. thi Man, C.A. Sewry, and G.E. Morris. 1996. The Emery-Dreifuss muscular dystrophy protein, emerin, is a nuclear membrane protein. Hum. Mol Genet. 5:801-808.
27. Matunis, M.J., J. Wu, and G. Blobel. 1998. SUMO-1 modification and its role in targeting the Ran GTPase-activating protein, RanGAP1, to the nuclear pore complex. J. Cell Biol. 140:499-509.
28. McKeon, F.D., M.W. Kirschner, and D. Caput. 1986. Homologies in both primary and secondary structure between nuclear envelope and intermediate filament proteins. Nature. 319:463-468.
29. 2+ based on green fluorescent proteins and calmodulin. Nature. 388:882-887.
30. Östlund, C., J. Ellenberg, E. Hallberg, J. Lippincott-Schwartz. and H.J. Worman. 1999. Intracellular trafficking of emerin, the Emery-Dreifuss muscular dystrophy protein. J. Cell Sci. 112:1709-1719.
31. Powell, L., and B. Burke. 1990. Internuclear exchange of an inner nuclear membrane protein (p55) in heterokaryons: in vivo evidence for the interaction of p55 with the nuclear lamina. J. Cell Biol. 111:2225-2234.
32. Sawin, K.E., and P. Nurse. 1996. Identification of fission yeast nuclear markers using random polypeptide fusions with green fluorescent protein. Proc. Natl. Acad. Sci. USA. 94:15146-15151.
33. Schuler, E., F. Lin, and H.J. Worman. 1994. Characterization of the human gene encoding LBR, an integral protein of the nuclear envelope inner membrane. J. Biol. Chem. 269:11312-11317.
34. Shamu, C. 1998. Splicing: HACking into the unfolded-protein response. Curr. Biol. 8:R121-R123.
35. Shibasaki, F., E.R. Price, D. Milan, and F. McKeon. 1996. Role of kinases and the phosphatase calcineurin in the nuclear shuttling of transcription factor NF-AT4. Nature. 382:370-373.
36. Silve, S., P.-H. Dupuy, P. Ferrara, and G. Loison. 1998. Human lamin B receptor exhibits sterol C14-reductasc activity in Saccharomyces cerevisiae. Biochim. Biophys. Acta. 1392:233-244.
37. Snow, C.M., A. Senior, and L. Gerace. 1987. Nuclear pore complex glycoproteins. J. Cell Biol. 104:1143-1156.
38. Söderqvist, H., G. Imreh, M. Kihlmark. C. Linnman, N. Ringertz, and E. Hallberg. 1997. Intracellular distribution of an integral pore membrane protein fused to green fluorescent protein: localization of a targeting domain. Eur. J. Biochem. 250:808-813.
39. Soullam, B., and H.J. Worman. 1993. The amino-terminal domain of the lamin B receptor is a nuclear envelope targeting signal. J. Cell Biol. 120: 1093-1100.
40. Spector, D.L., R.D. Goldman, and L.A. Leinwand. 1998. Cells: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
41. Stuurman, N., S. Heins, and U. Aebi. 1998. Nuclear lamins: their structure, assembly, and interactions. J. Struct. Biol. 122:42-66.
42. Torrisi, M.R., and S. Bonatti. 1985. Immunocytochemical study of the partition and distribution of Sindbis virus glycoproteins in freeze-fractured membranes of infected baby hamster kidney cells. J. Cell Biol. 101:1300-1306.
43. Worman, H.J., J. Yuan, G. Blobel, and S.D. Georgatos. 1988. A lamin B receptor in the nuclear envelope. Proc. Natl. Acad. Sci. USA. 85:8531-8534.
44. Wozniak, R.W., and G. Blobel. 1992. The single transmembrane segment of gp210 is sufficient for sorting to the pore membrane domain of the nuclear envelope. J. Cell Biol. 119:1441-1449.
45. Yang, L., T. Guan, and L. Gerace. 1997. Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the cell cycle and progression into S phase. J. Cell Biol. 139:1077-1087.
46. Ye, Q., and H.J. Worman. 1994. Primary structure analysis and lamin B and DNA binding of human LBR, and integral protein of the nuclear envelope inner membrane. J. Biol. Chem. 269:11306-11311.
47. Ye, Q., and H.J. Worman. 1996. Interaction between an integral protein of the nuclear envelope inner membrane and human chromodomain proteins homologous to Drosophila HP1. J. Biol. Chem. 271:14653-14656.