Improved green fluorescent protein by molecular evolution using DNA shuffling

Nature Biotechnology

Volumn 14, Issue 3, 1996, Pages

  Crameri, Andreas ^a   Whitehorn, Erik A ^a   Tate, Emily ^a   Stemmer, Willem P C ^a  

^a AFFYMAX RESEARCH INSTITUTE   (United States)

Author keywords

Molecular libraries; Protein expression; Sexual PCR

Indexed keywords

DNA; GREEN FLUORESCENT PROTEIN;

DNA SHUFFLING; MOLECULAR EVOLUTION; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DNA RECOMBINATION; ESCHERICHIA COLI; EUKARYOTE; EXPRESSION VECTOR; FLUORESCENCE MICROSCOPY; GENE EXPRESSION REGULATION; GENE LIBRARY; GENETIC ENGINEERING; MOLECULAR CLONING; NONHUMAN; PLASMID; PRIORITY JOURNAL; ULTRAVIOLET RADIATION;

ANIMALS; BASE SEQUENCE; BIOTECHNOLOGY; CHO CELLS; CRICETINAE; DIRECTED MOLECULAR EVOLUTION; DNA PRIMERS; DNA, RECOMBINANT; ESCHERICHIA COLI; FLUORESCENCE; GENE EXPRESSION; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MUTAGENESIS; POLYMERASE CHAIN REACTION; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

EID: 0029670330     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt0396-315     Document Type: Article

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