Motif Trap: A rapid method to clone motifs that can target proteins to defined subcellular localisations

Journal of Cell Science

Volumn 112, Issue 23, 1999, Pages 4207-4211

  Bejarano, Luis A ^a   González, Cayetano ^a  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

GFP; Protein targeting motif; Subcellular localisation; Visual screen

Indexed keywords

COMPLEMENTARY DNA; DNA FRAGMENT; GREEN FLUORESCENT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CELL ISOLATION; CELL ORGANELLE; EUKARYOTIC CELL; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN STRUCTURE; PROTEIN TARGETING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TECHNIQUE;

AMINO ACID SEQUENCE; CELL LINE; CLONING, MOLECULAR; DNA; DNA PRIMERS; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; MITOCHONDRIA; MOLECULAR SEQUENCE DATA; PEPTIDE LIBRARY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SUBCELLULAR FRACTIONS; TRANSFECTION;

EUKARYOTA;

EID: 0033491334     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (15)

1. Cannon, K. S. and Helenius, A. (1999). Trimming and readdition of glucose to N-linked oligosaccharides determines calnexin association of a substrate glycoprotein in living cells. J. Biol. Chem. 274, 7537-7544.
2. Chen, C. and Okayama, H. (1987). High-efficiency transformation of mammalian cells hy plasmid DNA. Mol. Cell Biol. 7, 2745-2752.
3. Cubitt, A. B., Heim, R., Adams, S. R., Boyd, A. E., Gross, L. A. and Tsien, R. Y. (1995). Understanding, improving and using green fluorescent proteins. Trends Biochem. Sci. 20, 448-455.
4. Cudmore, S. Reckmann, I. and Way, M. (1997). Viral manipulations of the actin cytoskeleton. Trend Microbiol. 5, 142-147.
5. Dingwall, C. and Laskey, R. A. (1991). Nuclear targeting sequences - a consensus? Trends Biochem. Sci. 16, 478-481.
6. Dotti, C. G. and Simmons, K. (1990). Polarized sorting of viral glycoproteins to the axon and dendrites of hippocampal neurons in culture. Cell 62, 63-72.
7. Hancock, J. F., Paterson, H. and Marshall, C. J. (1990). A polybasic domain or palmitoylation is required in addition to the CAAX motif to localize p21ras to the plasma membrane. Cell 63, 133-139.
8. Harlow, E. and Lane, D. (1988). Antibodies: a Laboratory Manual. Cold Spring Harbour Laboratory Press, NY.
9. Johnston, J. A., Ward, C. L. and Kopito, R. R. (1998). Aggresomes: a cellular response to misfolded proteins. J. Cell Biol. 143, 1883-1898.
10. Kislauskis, E. H. and Singer, R. H. (1992). Determinants of mRNA localization. Curr. Opin. Cell Biol. 4, 975-978.
11. Nacken, W., Klempt, M. and Sorg, C. (1995). The mouse homologue of the HTLV-1 tax responsive element binding protein TAXREB 107 is a highly conserved gene which may regulate some basal cellular functions. Biochim. Biophys. Acta 1261, 432-434.
12. Roise, D. and Schatz, G. (1988). Mitochondrial presequences. J. Biol. Chem. 63, 4509-4511.
13. Rolls, M. M., Stein, P. A., Taylor, S. S., Ha, E., McKeon, F. and Rapoport, T. A. (1999). A visual screen of a GFP-fusion library identifies a new type of nuclear envelope membrane protein. J. Cell Biol. 146, 29-43.
14. Sawin, K. E. and Nurse, P. (1996). Identification of fission yeast nuclear markers using random polypeptide fusions with green fluorescent protein. Proc. Nat. Acad. Sci. USA 94, 15146-15151.
15. von Heijne, G. (1992). Membrane protein structure prediction, hydrophobicity analysis and the positive-inside rule. J. Mol. Biol. 225, 487-494.