The C-terminal portion of RAG2 protects against transposition in vitro

EMBO Journal

Volumn 22, Issue 8, 2003, Pages 1931-1938

  Elkin, Sheryl K ^a   Matthews, Adam G ^a   Oettinger, Marjorie A ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

Hybrid joining; RAG1; RAG2; Transposition; V(D)J recombination

Indexed keywords

RAG1 PROTEIN; RAG2 PROTEIN;

ARTICLE; CARBOXY TERMINAL SEQUENCE; DNA CLEAVAGE; DNA TRANSPOSITION; GENETIC RECOMBINATION; IN VITRO STUDY; IN VIVO STUDY; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; RECEPTOR GENE;

DNA; DNA-BINDING PROTEINS; HOMEODOMAIN PROTEINS; HYBRIDIZATION, GENETIC; NUCLEIC ACID CONFORMATION; PROTEIN STRUCTURE, TERTIARY; RECOMBINATION, GENETIC;

EID: 0037447338     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/cdg184     Document Type: Article

References (39)

1. Agrawal, A., Eastman, Q.M. and Schatz, D.G. (1998) Transposition mediated by RAG1 and RAG2 and its implications for the evolution of the immune system. Nature, 394, 744-751.
2. Akamatsu, Y. et al. (2003) Deletion of the RAG2 C terminus leads to impaired lymphoid development in mice. Proc. Natl. Acad. Sci. USA, 100, 1209-1214.
3. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K. (1989) Current Protocols in Molecular Biology. Greene Publishing Associates-Wiley Interscience, New York, NY.
4. Bassing, C.H., Swat, W. and Alt, F.W. (2002) The mechanism and regulation of chromosomal V(D)J recombination. Cell, 109, Suppl., S45-S55.
5. Bogue, M.A., Wang, C., Zhu, C. and Roth, D.B. (1997) V(D)J recombination in Ku86-deficient mice: distinct effects on coding, signal and hybrid joint formation. Immunity, 7, 37-47.
6. Colman, P.M., Laver, W.G., Varghese, J.N., Baker, A.T., Tulloch, P.A., Air, G.M. and Webster, R.G. (1987) Three-dimensional structure of a complex of antibody with influenza virus neuraminidase. Nature, 326, 358-363.
7. Cuomo, C.A. and Oettinger, M.A. (1994) Analysis of regions of RAG-2 important for V(D)J recombination. Nucleic Acids Res., 22, 1810-1814.
8. Cuomo, C.A., Mundy, C.L. and Oettinger, M.A. (1996) DNA sequence and structure requirements for cleavage of V(D)J recombination signal sequences. Mol. Cell. Biol., 16, 5683-5690.
9. Eastman, Q.M., Leu, T.M. and Schatz, D.G. (1996) Initiation of V(D)J recombination in vitro obeying the 12/23 rule. Nature, 380, 85-88.
10. Gellert, M. (2002) V(D)J recombination: rag proteins, repair factors and regulation. Annu. Rev. Biochem., 71, 101-132.
11. Han, J.O., Steen, S.B. and Roth, D.B. (1997) Ku86 is not required for protection of signal ends or for formation of nonstandard V(D)J recombination products. Mol. Cell. Biol., 17, 2226-2234.
12. Hiom, K., Melek, M. and Gellert, M. (1998) DNA transposition by the RAG1 and RAG2 proteins: a possible source of oncogenic translocations. Cell, 94, 463-470.
13. Hopp, T.P., Prickett, K.S., Price, V., Libby, R.T., March, C.J., Cerretti, P., Urdal, D.L. and Conlon, P.J. (1988) A short polypeptide marker sequence useful for recombinant protein identification and purification. Biotechnology, 6, 1205-1210.
14. Jones, J.M. and Gellert, M. (2001) Intermediates in V(D)J recombination: a stable RAG1/2 complex sequesters cleaved RSS ends. Proc. Natl. Acad. Sci. USA, 98, 12926-12931.
15. Kim, D.R. and Oettinger, M.A. (1998) Functional analysis of coordinated cleavage in V(D)J recombination. Mol. Cell. Biol., 18, 4679-4688.
16. Kim, D.R., Dai, Y., Mundy, C.L., Yang, W. and Oettinger, M.A. (1999) Mutations of acidic residues in RAG1 define the active site of the V(D)J recombinase. Genes Dev., 13, 3070-3080.
17. Kirch, S.A., Sudarsanam, P. and Oettinger, M.A. (1996) Regions of RAG1 protein critical for V(D)J recombination. Eur. J. Immunol., 26, 886-891.
18. Kirch, S.A., Rathbun, G.A. and Oettinger, M.A. (1998) Dual role of RAG2 in V(D)J recombination: catalysis and regulation of ordered Ig gene assembly. EMBO J., 17, 4881-4886.
19. Lewis, S.M., Hesse, J.E., Mizuuchi, K. and Gellert, M. (1988) Novel strand exchanges in V(D)J recombination. Cell, 55, 1099-1107.
20. Liang, H.-E., Hsu, L.-Y., Cado, D., Cowell, L.G., Kelsoe, G. and Schlissel, M.S. (2002) The 'dispensable' portion of RAG2 is necessary for efficient V-to-DJ rearrangement during B and T cell development. Immunity, 17, 639-651.
21. Litman, G.W., Anderson, M.K. and Rast, J.P. (1999) Evolution of antigen binding receptors. Annu. Rev. Immunol., 17, 109-147.
22. McBlane, J.F., van Gent, D.C., Ramsden, D.A., Romeo, C., Cuomo, C.A., Gellert, M. and Oettinger, M.A. (1995) Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps. Cell, 83, 387-395.
23. Melek, M. and Gellert, M. (2000) RAG1/2-mediated resolution of transposition intermediates: two pathways and possible consequences. Cell, 101, 625-633.
24. Melek, M., Gellert, M. and van Gent, D.C. (1998) Rejoining of DNA by the RAG1 and RAG2 proteins. Science, 280, 301-303.
25. Morzycka-Wroblewska, E., Lee, F.E. and Desiderio, S.V. (1988) Unusual immunoglobulin gene rearrangement leads to replacement of recombinational signal sequences. Science, 242, 261-263.
26. Mundy, C.L., Patenge, N., Matthews, A.G. and Oettinger, M.A. (2002) Assembly of the RAG1/RAG2 synaptic complex. Mol. Cell. Biol., 22, 69-77.
27. Neiditch, M.B., Lee, G.S., Landree, M.A. and Roth, D.B. (2001) RAG transposase can capture and commit to target DNA before or after donor cleavage. Mol. Cell. Biol., 21, 4302-4310.
28. Noordzij, J.G., Verkaik, N.S., Hartwig, N.G., de Groot, R., van Gent, D.C. and van Dongen, J.J. (2000) N-terminal truncated human RAG1 proteins can direct T-cell receptor but not immunoglobulin gene rearrangements. Blood, 96, 203-209.
29. Peixoto, B.R., Mikawa, Y. and Brenner, S. (2000) Characterization of the recombinase activating gene-1 and 2 locus in the Japanese pufferfish, Fugu rubripes. Gene, 246, 275-283.
30. -/- B cells reveals a requirement for novel elements in the N-terminus of RAG-1. Immunity, 7, 13-24.
31. Sadofsky, M.J., Hesse, J.E., McBlane, J.F. and Gellert, M. (1993) Expression and V(D)J recombination activity of mutated RAG-1 proteins. Nucleic Acids Res., 21, 5644-5650.
32. Sadofsky, M.J., Hesse, J.E. and Gellert, M. (1994) Definition of a core region of RAG-2 that is functional in V(D)J recombination. Nucleic Acids Res., 22, 1805-1809.
33. Santagata, S., Gomez, C.A., Sobacchi, C., Bozzi, F., Abinun, M., Pasic, S., Cortes, P., Vezzoni, P. and Villa, A. (2000) N-terminal RAG1 frameshift mutations in Omenn's syndrome: internal methionine usage leads to partial V(D)J recombination activity and reveals a fundamental role in vivo for the N-terminal domains. Proc. Natl. Acad. Sci. USA, 97, 14572-14577.
34. Schlissel, M.S. (2002) Does artemis end the hunt for the hairpin-opening activity in V(D)J recombination? Cell, 109, 1-4.
35. Sekiguchi, J.A., Whitlow, S. and Alt, F.W. (2001) Increased accumulation of hybrid V(D)J joins in cells expressing truncated versus full-length RAGs. Mol. Cell, 8, 1383-1390.
36. Silver, D.P., Spanopoulou, E., Mulligan, R.C. and Baltimore, D. (1993) Dispensable sequence motifs in the RAG-1 and RAG-2 genes for plasmid V(D)J recombination. Proc. Natl. Acad. Sci. USA, 90, 6100-6104.
37. Steen, S.B., Han, J.O., Mundy, C., Oettinger, M.A. and Roth, D.B. (1999) Roles of the 'dispensable' portions of RAG-1 and RAG-2 in V(D)J recombination. Mol. Cell. Biol., 19, 3010-3017.
38. Tsai, C.L., Drejer, A.H. and Schatz, D.G. (2002) Evidence of a critical architectural function for the RAG proteins in end processing, protection and joining in V(D)J recombination. Genes Dev., 16, 1934-1949.
39. van Gent, D.C., Ramsden, D.A. and Gellert, M. (1996) The RAG1 and RAG2 proteins establish the 12/23 rule in V(D)J recombination. Cell, 85, 107-113.