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Volumn 18, Issue 2, 2003, Pages 159-166

Statistics of the Comet assay: A key to discriminate between genotoxic effects

Author keywords

[No Author keywords available]

Indexed keywords

AMINOLEVULINIC ACID; CHEMICAL AGENT; PORPHYRIN;

EID: 0037342116     PISSN: 02678357     EISSN: None     Source Type: Journal    
DOI: 10.1093/mutage/18.2.159     Document Type: Article
Times cited : (138)

References (56)
  • 2
    • 0031021024 scopus 로고    scopus 로고
    • The comet assay as a repair test for prenatal diagnosis of xeroderma pigmentosum and trichothiodystrophy
    • Alapetite,C., Benoit,A., Moustacchi,E. and Sarasin,A. (1997) The comet assay as a repair test for prenatal diagnosis of xeroderma pigmentosum and trichothiodystrophy. J. Invest. Dermatol., 108, 154-159.
    • (1997) J. Invest. Dermatol. , vol.108 , pp. 154-159
    • Alapetite, C.1    Benoit, A.2    Moustacchi, E.3    Sarasin, A.4
  • 3
    • 0034255796 scopus 로고    scopus 로고
    • IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans
    • Albertini,R.J., Anderson,D., Douglas,G.R. et al. (2000) IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans. Mutat. Res., 463, 111-172.
    • (2000) Mutat. Res. , vol.463 , pp. 111-172
    • Albertini, R.J.1    Anderson, D.2    Douglas, G.R.3
  • 4
    • 0028305534 scopus 로고
    • The effect of various antioxidants and other modifying agents on oxygen-radical-generated DNA damage in human lymphocytes in the COMET assay
    • Anderson,D., Yu,T.W., Phillips,B.J. and Schmezer,P. (1994) The effect of various antioxidants and other modifying agents on oxygen-radical-generated DNA damage in human lymphocytes in the COMET assay. Mutat. Res., 307, 261-271.
    • (1994) Mutat. Res. , vol.307 , pp. 261-271
    • Anderson, D.1    Yu, T.W.2    Phillips, B.J.3    Schmezer, P.4
  • 6
    • 0031755770 scopus 로고    scopus 로고
    • Comet assay responses as indicators of carcinogen exposure
    • Anderson,D., Yu,T.W. and McGregor,D.B. (1998) Comet assay responses as indicators of carcinogen exposure. Mutagenesis, 13, 539-555.
    • (1998) Mutagenesis , vol.13 , pp. 539-555
    • Anderson, D.1    Yu, T.W.2    McGregor, D.B.3
  • 8
    • 0028910894 scopus 로고
    • The single cell gel electrophoresis assay for induced DNA damage (comet assay): Measurement of tail length and moment
    • Ashby,J., Tinwell,H., Lefevre,P.A. and Browe,M.A. (1995) The single cell gel electrophoresis assay for induced DNA damage (comet assay): measurement of tail length and moment, Mutagenesis, 10, 85-90.
    • (1995) Mutagenesis , vol.10 , pp. 85-90
    • Ashby, J.1    Tinwell, H.2    Lefevre, P.A.3    Browe, M.A.4
  • 10
    • 0032584623 scopus 로고    scopus 로고
    • Development and validation of the in vivo alkaline comet assay for detecting genomic damage in marine flatfish
    • Belpaeme,K., Cooreman,K. and Kirsch-Volders,M. (1998) Development and validation of the in vivo alkaline comet assay for detecting genomic damage in marine flatfish. Mutat. Res., 415, 167-184.
    • (1998) Mutat. Res. , vol.415 , pp. 167-184
    • Belpaeme, K.1    Cooreman, K.2    Kirsch-Volders, M.3
  • 11
    • 0032429174 scopus 로고    scopus 로고
    • Evaluation of radiation-induced DNA damage and DNA repair in human lung cancer cell lines with different radiosensitivity using alkaline and neutral single cell gel electrophoresis
    • Bergqvist,M., Brattstrom,D., Stalberg,M., Vaghef,H., Brodin,O. and Hellman,B. (1998) Evaluation of radiation-induced DNA damage and DNA repair in human lung cancer cell lines with different radiosensitivity using alkaline and neutral single cell gel electrophoresis. Cancer Lett., 133, 9-18.
    • (1998) Cancer Lett. , vol.133 , pp. 9-18
    • Bergqvist, M.1    Brattstrom, D.2    Stalberg, M.3    Vaghef, H.4    Brodin, O.5    Hellman, B.6
  • 12
    • 0028284199 scopus 로고
    • Microgel electrophoresis assay (comet test) and SCE analysis in human lymphocytes from 100 normal subjects
    • Betti,C., Davini,T., Giannessi,L., Loprieno,N. and Barale,R. (1994) Microgel electrophoresis assay (comet test) and SCE analysis in human lymphocytes from 100 normal subjects. Mutat. Res., 307, 323-333.
    • (1994) Mutat. Res. , vol.307 , pp. 323-333
    • Betti, C.1    Davini, T.2    Giannessi, L.3    Loprieno, N.4    Barale, R.5
  • 13
    • 0031879190 scopus 로고    scopus 로고
    • Comet assay detects cold repair of UV-A damages in a human B-lymphoblast cell line
    • Bock,C., Dittmar,H., Gemeinhardt,H., Bauer,E. and Greulich,K.O. (1998) Comet assay detects cold repair of UV-A damages in a human B-lymphoblast cell line. Mutat. Res., 408, 111-120.
    • (1998) Mutat. Res. , vol.408 , pp. 111-120
    • Bock, C.1    Dittmar, H.2    Gemeinhardt, H.3    Bauer, E.4    Greulich, K.O.5
  • 15
    • 0031557167 scopus 로고    scopus 로고
    • Validation of chromatographic methods in biomedical analysis. Viewpoint and discussion
    • Causon,R. (1997) Validation of chromatographic methods in biomedical analysis. Viewpoint and discussion. J. Chromatogr. B Biomed. Appl., 689, 175-180.
    • (1997) J. Chromatogr. B Biomed. Appl. , vol.689 , pp. 175-180
    • Causon, R.1
  • 16
    • 0029015296 scopus 로고
    • Rat mesothelial and tracheal epithelial cells show equal DNA sensitivity to hydrogen peroxide-induced oxidant injury
    • Churg,A., Keeling,B., Gilks,B., Porter,S. and Olive,P. (1995) Rat mesothelial and tracheal epithelial cells show equal DNA sensitivity to hydrogen peroxide-induced oxidant injury. Am. J. Physiol., 268, L832-L838.
    • (1995) Am. J. Physiol. , vol.268
    • Churg, A.1    Keeling, B.2    Gilks, B.3    Porter, S.4    Olive, P.5
  • 17
    • 0036043770 scopus 로고    scopus 로고
    • The comet assay. Principles, applications and limitations
    • Collins,A.R. (2002) The comet assay. Principles, applications and limitations. Methods Mol. Biol., 203, 163-177.
    • (2002) Methods Mol. Biol. , vol.203 , pp. 163-177
    • Collins, A.R.1
  • 18
    • 0030014152 scopus 로고    scopus 로고
    • Oxidative damage to DNA: Do we have a reliable biomarker?
    • Collins,A.R., Dusinska,M., Gedik,C.M. and Stetina,R. (1996) Oxidative damage to DNA: do we have a reliable biomarker? Environ. Health Perspect., 104 (suppl. 3), 465-469.
    • (1996) Environ. Health Perspect. , vol.104 , Issue.SUPPL. 3 , pp. 465-469
    • Collins, A.R.1    Dusinska, M.2    Gedik, C.M.3    Stetina, R.4
  • 21
    • 0342904908 scopus 로고    scopus 로고
    • Comet assay in genetic ecotoxicology: A review
    • Cotelle,S. and Ferard,J.F. (1999) Comet assay in genetic ecotoxicology: a review. Environ. Mol. Mutagen., 34, 246-255.
    • (1999) Environ. Mol. Mutagen. , vol.34 , pp. 246-255
    • Cotelle, S.1    Ferard, J.F.2
  • 22
  • 23
    • 0035007619 scopus 로고    scopus 로고
    • Photodynamic DNA damage mediated by δ-aminolevulinic acid-induced porphyrins
    • Duez,P., Hanocq,M. and Dubois,J. (2001) Photodynamic DNA damage mediated by δ-aminolevulinic acid-induced porphyrins. Carcinogenesis, 22, 101-109.
    • (2001) Carcinogenesis , vol.22 , pp. 101-109
    • Duez, P.1    Hanocq, M.2    Dubois, J.3
  • 25
    • 0026348625 scopus 로고
    • Apoptosis: Molecular mechanisms in programmed cell death
    • Fesus,L., Davies,P.J. and Piacentini,M. (1991) Apoptosis: molecular mechanisms in programmed cell death. Eur. J. Cell Biol., 56, 170-177.
    • (1991) Eur. J. Cell Biol. , vol.56 , pp. 170-177
    • Fesus, L.1    Davies, P.J.2    Piacentini, M.3
  • 26
    • 0026738581 scopus 로고
    • Single-cell gel electrophoresis applied to the analysis of UV-C damage and its repair in human cells
    • Gedik,C.M., Ewen,S.W. and Collins,A.R. (1992) Single-cell gel electrophoresis applied to the analysis of UV-C damage and its repair in human cells. Int. J. Radiat. Biol., 62, 313-320.
    • (1992) Int. J. Radiat. Biol. , vol.62 , pp. 313-320
    • Gedik, C.M.1    Ewen, S.W.2    Collins, A.R.3
  • 27
    • 0032460862 scopus 로고    scopus 로고
    • Measuring oxidative damage to DNA; HPLC and the comet assay compared
    • Gedik,C.M., Wood,S.G. and Collins,A.R. (1998) Measuring oxidative damage to DNA; HPLC and the comet assay compared. Free Radic. Res., 29, 609-615.
    • (1998) Free Radic. Res. , vol.29 , pp. 609-615
    • Gedik, C.M.1    Wood, S.G.2    Collins, A.R.3
  • 28
    • 0028956450 scopus 로고
    • The concepts of tail moment and tail inertia in the single cell gel electrophoresis assay
    • Hellman,B., Vaghef,H. and Boström,B. (1995) The concepts of tail moment and tail inertia in the single cell gel electrophoresis assay. Mutat. Res., 336, 123-131.
    • (1995) Mutat. Res. , vol.336 , pp. 123-131
    • Hellman, B.1    Vaghef, H.2    Boström, B.3
  • 29
    • 0029085953 scopus 로고
    • Reproducibility of basal and induced DNA single-strand breaks detected by the single-cell gel electrophoresis assay in human peripheral mononuclear leukocytes
    • Holz,O., Jorres,R., Kastner,A., Krause,T. and Magnussen,H. (1995) Reproducibility of basal and induced DNA single-strand breaks detected by the single-cell gel electrophoresis assay in human peripheral mononuclear leukocytes. Int. Arch. Occup. Environ. Health, 67, 305-310.
    • (1995) Int. Arch. Occup. Environ. Health , vol.67 , pp. 305-310
    • Holz, O.1    Jorres, R.2    Kastner, A.3    Krause, T.4    Magnussen, H.5
  • 30
    • 0034213140 scopus 로고    scopus 로고
    • Single cell gel electrophoresis assay: A new technique for human biomonitoring studies
    • Kassie,F., Parzefall,W. and Knasmuller,S. (2000) Single cell gel electrophoresis assay: a new technique for human biomonitoring studies. Mutat. Res., 463, 13-31.
    • (2000) Mutat. Res. , vol.463 , pp. 13-31
    • Kassie, F.1    Parzefall, W.2    Knasmuller, S.3
  • 31
    • 0029045164 scopus 로고
    • The comet moment as a measure of DNA damage in the comet assay
    • Kent,C.R., Eady,J.J., Ross,G.M. and Steel,G.G. (1995) The comet moment as a measure of DNA damage in the comet assay. Int. J. Radiat. Biol., 67, 655-660.
    • (1995) Int. J. Radiat. Biol. , vol.67 , pp. 655-660
    • Kent, C.R.1    Eady, J.J.2    Ross, G.M.3    Steel, G.G.4
  • 32
    • 0030580947 scopus 로고    scopus 로고
    • The comet assay: Mechanisms and technical considerations
    • Klaude,M., Eriksson,S., Nygren,J. and Ahnstrom,G. (1996) The comet assay: mechanisms and technical considerations. Mutat. Res., 363, 89-96.
    • (1996) Mutat. Res. , vol.363 , pp. 89-96
    • Klaude, M.1    Eriksson, S.2    Nygren, J.3    Ahnstrom, G.4
  • 34
    • 0031776788 scopus 로고    scopus 로고
    • Kinetics of DNA strand breaks and protection by antioxidants in UVA- or UVB-irradiated HaCaT keratinocytes using the single cell gel electrophoresis assay
    • Lehmann,J., Pollet,D., Peker,S., Steinkraus,V. and Hoppe,U. (1998) Kinetics of DNA strand breaks and protection by antioxidants in UVA- or UVB-irradiated HaCaT keratinocytes using the single cell gel electrophoresis assay. Mutat. Res., 407, 97-108.
    • (1998) Mutat. Res. , vol.407 , pp. 97-108
    • Lehmann, J.1    Pollet, D.2    Peker, S.3    Steinkraus, V.4    Hoppe, U.5
  • 35
    • 0032940837 scopus 로고    scopus 로고
    • Issues related to the experimental design and subsequent statistical analysis of in vivo and in vitro comet studies
    • Lovell,D.P., Thomas,G. and Dunbow,R. (1999) Issues related to the experimental design and subsequent statistical analysis of in vivo and in vitro comet studies. Teratog. Carcinog. Mutagen., 19, 109-119.
    • (1999) Teratog. Carcinog. Mutagen. , vol.19 , pp. 109-119
    • Lovell, D.P.1    Thomas, G.2    Dunbow, R.3
  • 36
    • 0033501585 scopus 로고    scopus 로고
    • New approaches to estimating the mutagenic potential of chemicals
    • Marzin,D. (1999) New approaches to estimating the mutagenic potential of chemicals. Cell Biol. Toxicol., 15, 359-365.
    • (1999) Cell Biol. Toxicol. , vol.15 , pp. 359-365
    • Marzin, D.1
  • 37
    • 0032960477 scopus 로고    scopus 로고
    • Detection of crosslinks with the comet assay in relationship to genotoxicity and cytotoxicity
    • Merk,O. and Speit,G. (1999) Detection of crosslinks with the comet assay in relationship to genotoxicity and cytotoxicity. Environ. Mol. Mutagen., 33, 167-172.
    • (1999) Environ. Mol. Mutagen. , vol.33 , pp. 167-172
    • Merk, O.1    Speit, G.2
  • 38
    • 0030692118 scopus 로고    scopus 로고
    • Detection of DNA lesions induced by chemical mutagens by the single cell gel electrophoresis (Comet) assay. 1. Relationship between the onset of DNA damage and the characteristics of mutagens
    • Miyamae,Y., Zaizen,K., Ohara,K., Mine,Y. and Sasaki,Y.F. (1997) Detection of DNA lesions induced by chemical mutagens by the single cell gel electrophoresis (Comet) assay. 1. Relationship between the onset of DNA damage and the characteristics of mutagens. Mutat. Res., 393, 99-106.
    • (1997) Mutat. Res. , vol.393 , pp. 99-106
    • Miyamae, Y.1    Zaizen, K.2    Ohara, K.3    Mine, Y.4    Sasaki, Y.F.5
  • 39
    • 0033790136 scopus 로고    scopus 로고
    • The comet assay as a rapid test in biomonitoring occupational exposure to DNA-damaging agents and effect of confounding factors
    • Moller,P., Knudsen,L.E., Loft,S. and Wallin,H. (2000) The comet assay as a rapid test in biomonitoring occupational exposure to DNA-damaging agents and effect of confounding factors. Cancer Epidemiol. Biomarkers Prev., 9, 1005-1015.
    • (2000) Cancer Epidemiol. Biomarkers Prev. , vol.9 , pp. 1005-1015
    • Moller, P.1    Knudsen, L.E.2    Loft, S.3    Wallin, H.4
  • 40
    • 0029583789 scopus 로고
    • Comparison of the microgel electrophoresis assay and other assays for genotoxicity in the detection of DNA damage
    • Monteith,D.K. and Vanstone,J. (1995) Comparison of the microgel electrophoresis assay and other assays for genotoxicity in the detection of DNA damage. Mutat. Res., 345, 97-103.
    • (1995) Mutat. Res. , vol.345 , pp. 97-103
    • Monteith, D.K.1    Vanstone, J.2
  • 42
    • 0032959983 scopus 로고    scopus 로고
    • DNA damage and repair in individual cells: Applications of the comet assay in radiobiology
    • Olive,P.L. (1999) DNA damage and repair in individual cells: applications of the comet assay in radiobiology. Int. J. Radiat. Biol., 75, 395-405.
    • (1999) Int. J. Radiat. Biol. , vol.75 , pp. 395-405
    • Olive, P.L.1
  • 43
    • 0026659839 scopus 로고
    • Detection of hypoxic cells in a murine tumor with the use of the comet assay
    • Olive,P.L. and Durand,R.E. (1992) Detection of hypoxic cells in a murine tumor with the use of the comet assay. J. Natl Cancer Inst., 84, 707-711.
    • (1992) J. Natl. Cancer Inst. , vol.84 , pp. 707-711
    • Olive, P.L.1    Durand, R.E.2
  • 44
    • 0025270234 scopus 로고
    • Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the 'comet' assay
    • Olive,P.L., Banath,J.P. and Durand,R.E. (1990) Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the 'comet' assay. Radiat. Res., 122, 86-94.
    • (1990) Radiat. Res. , vol.122 , pp. 86-94
    • Olive, P.L.1    Banath, J.P.2    Durand, R.E.3
  • 45
    • 0031939561 scopus 로고    scopus 로고
    • The comet assay: A new method to examine heterogeneity associated with solid tumors
    • Olive,P.L., Johnston,P.J., Banath,J.P. and Durand,R.E. (1998) The comet assay: a new method to examine heterogeneity associated with solid tumors. Nature Med., 4, 103-105.
    • (1998) Nature Med. , vol.4 , pp. 103-105
    • Olive, P.L.1    Johnston, P.J.2    Banath, J.P.3    Durand, R.E.4
  • 46
    • 0030012151 scopus 로고    scopus 로고
    • Detection of DNA-crosslinking agents with the alkaline comet assay
    • Pfuhler,S. and Wolf,H.U. (1996) Detection of DNA-crosslinking agents with the alkaline comet assay. Environ. Mol. Mutagen., 27, 196-201.
    • (1996) Environ. Mol. Mutagen. , vol.27 , pp. 196-201
    • Pfuhler, S.1    Wolf, H.U.2
  • 47
    • 0033067120 scopus 로고    scopus 로고
    • Single cell gel electrophoresis assay: Methodology and applications
    • Rojas,E., Lopez,M.C. and Valverde,M. (1999) Single cell gel electrophoresis assay: methodology and applications. J. Chromatogr. B Biomed. Appl., 722, 225-254.
    • (1999) J. Chromatogr. B Biomed. Appl. , vol.722 , pp. 225-254
    • Rojas, E.1    Lopez, M.C.2    Valverde, M.3
  • 48
    • 0033060055 scopus 로고    scopus 로고
    • The alkaline single cell gel electrophoresis assay with mouse multiple organs: Results with 30 aromatic amines evaluated by the IARC and U.S. NTP
    • Sasaki,Y.F., Fujikawa,K., Ishida,K. et al. (1999) The alkaline single cell gel electrophoresis assay with mouse multiple organs: results with 30 aromatic amines evaluated by the IARC and U.S. NTP. Mutat. Res., 440, 1-18.
    • (1999) Mutat. Res. , vol.440 , pp. 1-18
    • Sasaki, Y.F.1    Fujikawa, K.2    Ishida, K.3
  • 49
    • 0000898845 scopus 로고
    • An analysis of variance test for normality (complete samples)
    • Shapiro,S.S. and Wilk,M.B. (1965) An analysis of variance test for normality (complete samples). Biometrika, 52, 591-611.
    • (1965) Biometrika , vol.52 , pp. 591-611
    • Shapiro, S.S.1    Wilk, M.B.2
  • 50
    • 0023919963 scopus 로고
    • A simple technique for quantitation of low levels of DNA damage in individual cells
    • Singh,N.P., McCoy,M.T., Tice,R.R. and Schneider,E.L. (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res., 175, 184-191.
    • (1988) Exp. Cell Res. , vol.175 , pp. 184-191
    • Singh, N.P.1    McCoy, M.T.2    Tice, R.R.3    Schneider, E.L.4
  • 51
    • 0028865604 scopus 로고
    • The contribution of excision repair to the DNA effects seen in the alkaline single cell gel test (comet assay)
    • Speit,G. and Hartmann,A. (1995) The contribution of excision repair to the DNA effects seen in the alkaline single cell gel test (comet assay). Mutagenesis, 10, 555-559.
    • (1995) Mutagenesis , vol.10 , pp. 555-559
    • Speit, G.1    Hartmann, A.2
  • 52
    • 0004274431 scopus 로고    scopus 로고
    • Statsoft Inc. Statsoft. Available at
    • Statsoft Inc. (1999) Electronic Statistics Textbook, Statsoft. Available at http://www.statsoft.com/textbook/stathome.html.
    • (1999) Electronic Statistics Textbook
  • 54
    • 0026751636 scopus 로고
    • Assessment of radiation-induced DNA damage in human blood lymphocytes using the single-cell gel electrophoresis technique
    • Vijayalaxmi, Tice,R.R. and Strauss,G.H. (1992) Assessment of radiation-induced DNA damage in human blood lymphocytes using the single-cell gel electrophoresis technique. Mutat. Res., 271, 243-252.
    • (1992) Mutat. Res. , vol.271 , pp. 243-252
    • Vijayalaxmi, A.1    Tice, R.R.2    Strauss, G.H.3
  • 55
    • 0344301939 scopus 로고    scopus 로고
    • Application of the comet assay for monitoring DNA damage in workers exposed to chronic low-dose irradiation. I. Strand breakage
    • Wojewodzka,M., Kruszewski,M., Iwanenko,T., Collins,A.R. and Szumiel,I. (1998) Application of the comet assay for monitoring DNA damage in workers exposed to chronic low-dose irradiation. I. Strand breakage. Mutat. Res., 416, 21-35.
    • (1998) Mutat. Res. , vol.416 , pp. 21-35
    • Wojewodzka, M.1    Kruszewski, M.2    Iwanenko, T.3    Collins, A.R.4    Szumiel, I.5
  • 56
    • 0004252445 scopus 로고    scopus 로고
    • 3rd Edn. Prentice Hall, Upper Saddle River, NJ
    • Zar,J.H. (1996) Biostatistical Analysis, 3rd Edn. Prentice Hall, Upper Saddle River, NJ.
    • (1996) Biostatistical Analysis
    • Zar, J.H.1


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