Validation and implementation of an internal standard in comet assay analysis

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 469, Issue 2, 2000, Pages 181-197

  De Boeck, Marlies ^a   Touil, Nadia ^a   De Visscher, Geofrey ^a   Vande, Peter Aka ^a   Kirsch Volders, Micheline ^a  

^a VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

Author keywords

Comet assay; DNA damage; Interelectrophoresis and interexperimenter variation; Internal standard

Indexed keywords

ARTICLE; CELL STRAIN K 562; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; ELECTROPHORESIS; ERYTHROLEUKEMIA CELL; GENOTOXICITY; HUMAN; HUMAN CELL; METHODOLOGY; OBSERVER VARIATION; PRIORITY JOURNAL; STANDARD; VALIDATION PROCESS;

COMET ASSAY; DNA DAMAGE; ETHYL METHANESULFONATE; HUMANS; K562 CELLS; LYMPHOCYTES; MUTAGENS; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS;

EID: 0034692623     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1383-5718(00)00075-9     Document Type: Article

References (14)

1. Singh N.P., McCoy M.T., Tice R.R., Schneider E.L. A simple technique for quantification of low levels of DNA damage in individual cells. Exp. Cell Res. 175:1988;184-191.
2. Tice R.R., Agurell E., Anderson D., Burlinson B., Hartmann A., Kobayashi H., Miyamae Y., Rojas E., Ryu J., Sasaki Y.F. Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen. 35:2000;206-221.
3. Vaghef H., Nygren P., Edling C., Bergh J., Hellman B. Alkaline single-cell gel electrophoresis and human biomonitoring for genotoxicity: a pilot study on breast cancer patients undergoing chemotherapy including cyclophosphamide. Mutat. Res. 395:1997;127-138.
4. Lebailly P., Vigreux C., Lechevrel C., Ledemeney D., Godard T., Sichel F., LeTalaër J.Y., Henry-Amar M., Gauduchon P. DNA damage in mononuclear leukocytes of farmers measured using the alkaline comet assay: discussion of critical parameters and evaluation of seasonal variations in relation to pesticide exposure. Cancer Epidemiol. Biomarkers and Prevention. 7:1998;917-927.
5. Lozzio C.B., Lozzio B.B. Human chronic myelogenous leukemia cell line with positive Philadelphia chromosome. Blood. 45:1975;321-334.
6. Telerman A., Tuynder M., Dupression T., Robaye B., Sigaux F., Shaulian E., Oren M., Rommelaere J., Amson R. A model for tumor suppression by H-1 parvovirus. Proc. Natl. Acad. Sci. U.S.A. 90:1993;8702-8706.
7. Casenghi M., Mangiacasale R., Tuynder M., Caillet-Fauquet P., Elhajouji A., Lavia P., Mousset S., Kirsch-Volders M., Cundari E. p53-Independent apoptosis and p53-dependent block of DNA rereplication following mitotic spindle inhibition in human cells. Exp. Cell Res. 250:1999;339-350.
8. Verdoodt B., Decordier I., Geleyns K., Cunha M., Cundari E., Kirsch-Volders M. Induction of polyploidy and apoptosis after exposure to high concentrations of the spindle poison nocodazole. Mutagenesis. 14(5):1999;513-520.
9. De Boeck M., Lison D., Kirsch-Volders M. Evaluation of the in vitro direct and indirect genotoxic effects of cobalt compounds using the alkaline comet assay. Influence of interdonor and interexperimental variability. Carcinogenesis. 19:1998;2021-2029.
10. Anderson D., Yu T., McGregor D.B. Comet assay responses as indicators of carcinogen exposure. Mutagenesis. 13:1998;539-555.
11. Belpaeme K., Cooreman K., Kirsch-Volders M. Development and validation of the in vivo alkaline comet assay for detecting genomic damage in marine flatfish. Mutat. Res. 415:1998;167-184.
12. M. De Boeck, S. Lardau, M. Kirsch-Volders, D. Lison, Absence of significant genotoxicity in lymphocytes and urine from workers exposed to moderate levels of cobalt-cotaining dust: A cross-sectional study, Environ. Mol. Mutagen. (2000) in press.
13. N. Touil, P.V. Aka, H. Thierens, J.P. Buchet, M. Kirsch-Volders, Assessment of Genetic Risk Related to Chronic Low Level Exposure to Ionising Radiation Using Biological Markers, in preparation.
14. Alapetite C., Thirion P., De la Rocheforière A., Cosset J., Moustacchi E. Analysis by alkaline comet assay of cancer patients with severe reactions to radiotherapy: defective rejoining of radioinduced DNA strand breaks in lymphocytes of breast cancer patients. Int. J. Cancer. 83:1999;83-90.