Detection of DNA lesions induced by chemical mutagens by the single cell gel electrophoresis (Comet) assay. 1. Relationship between the onset of DNA damage and the characteristics of mutagens

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 393, Issue 1-2, 1997, Pages 99-106

  Miyamae, Youichi ^a   Zaizen, Kazuyo ^a   Ohara, Kaname ^a   Mine, Yasuhiro ^a   Sasaki, Yu F ^b  

^a ASTELLAS PHARMA INC   (Japan)

^b HACHINOHE NATIONAL COLLEGE OF TECHNOLOGY   (Japan)

Author keywords

Chemical mutagen; Comet assay; DNA lesion; Single cell gel electrophoresis (SCG) assay

Indexed keywords

BLEOMYCIN; CHEMICAL MUTAGEN; MERCAPTOPURINE; MESYLIC ACID METHYL ESTER; METHYLNITROSOUREA; MITOMYCIN;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CYTOTOXICITY; DNA DAMAGE; GEL ELECTROPHORESIS; GENOTOXICITY; LYMPHOMA CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL;

6-MERCAPTOPURINE; ANIMALS; BLEOMYCIN; CROSS-LINKING REAGENTS; DNA DAMAGE; ELECTROPHORESIS; LEUKEMIA L5178; METHYL METHANESULFONATE; METHYLNITROSOUREA; MICE; MITOMYCIN; MUTAGENICITY TESTS; MUTAGENS; NUCLEIC ACID SYNTHESIS INHIBITORS; TIME FACTORS; TUMOR CELLS, CULTURED; ULTRAVIOLET RAYS; X-RAYS;

ANIMALIA;

EID: 0030692118     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1383-5718(97)00090-9     Document Type: Article

References (15)

1. Singh N.P., McCoy M.T., Tice R.R., Schneider E.L. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell. Res. 175:1988;184-191.
2. Fairbairn D.W., Olive P.L., O'Neill K.L. The comet assay: a comprehensive review. Mutation Res. 339:1995;37-59.
3. Vijayalaxmi, Tice R.R., Strauss G.H.S. Assessment of radiation-induced DNA damage in human blood lymphocytes using the single-cell gell electrophoresis technique. Mutation Res. 271:1992;243-252.
4. McKelvey V.J., Tice R.R., Singh N.P. A Technique for measuring DNA single strand breaks and alkali labile sites in individual cells. Br. J. Cancer. 62:1990;529-530.
5. Olive P.L., Wlodek D., Durand R.E., Banáth J.P. Factors influencing DNA migration from individual cells subjected to gel electrophoresis. Exp. Cell Res. 198:1992;259-267.
6. Pfuhler S., Wolf H.U. Detection of DNA-crosslinking agents with the alkaline comet assay. Environ. Mol. Mutagen. 27:1996;196-201.
7. M. Ishidate, Jr., Data Book of Chromosomal Aberration Test In Vitro, Life-Science Information Center Press, 1987.
8. Friedberg E.C., Ehmann U.K., Williams J.I. Human diseases associated with defective DNA repair. Adv. Radiat. Biol. 8:1979;85-174.
9. Hanawalt P.C., Cooper P.K., Ganesan A.K., Smith C.A. DNA repair in bacteria and mammalian cells. Annu. Rev. Biochem. 48:1979;783-803.
10. Vig B.K., Lewis R. Genetic toxicology of bleomycin. Mutation Res. 49:1978;189-238.
11. Szybalski W. Crosslinking of DNA by enzymatically or chemically activated mitomycins and porfiromycins, bifunctionally alkylating antibiotics. Fed. Proc. 23:1964;946-957.
12. Miyamae Y., Iwasaki K., Kinae N., Tsuda S., Murakami M., Tanaka M., Sasaki Y. Detection of DNA lesions induced by chemical mutagens using the single-cell gel electrophoresis (Comet) assay: relationship between DNA migration and alkaline condition. Mutation. Res. 393:1997;107-113.
13. Olive, Frazer G., Bançth J.P. Radiation-induced apoptosis measured in TK6 human B lymphoblast cells using the comet assay. Radiat. Res. 136:1993;130-136.
14. ± mouse lymphoma mutagen assay system. Mutation Res. 59:1979;61-108.
15. Van Scoik K.G., Johnson A. The pharmacology and mechanism of the thiopurine drugs 6-mercaptopurine and azathioprine. Drug Metab. Rev. 16:1985;157-174.