The comet assay: Mechanisms and technical considerations

Mutation Research - DNA Repair

Volumn 363, Issue 2, 1996, Pages 89-96

  Klaude, Maria ^a   Eriksson, Stefan ^a   Nygren, Jonas ^a   Ahnström, Gunnar ^a  

^a STOCKHOLM UNIVERSITY   (Sweden)

Author keywords

Comet assay; Microautoradiography; Single cell gel electrophoresis

Indexed keywords

DNA; SODIUM CHLORIDE;

AGAR GEL ELECTROPHORESIS; ANIMAL CELL; ARTICLE; AUTORADIOGRAPHY; CHO CELL; CONTROLLED STUDY; DNA DAMAGE; GENETIC PROCEDURES; HUMAN CELL; PH; PRIORITY JOURNAL;

ANIMALIA; MAMMALIA;

EID: 0030580947     PISSN: 09218777     EISSN: None     Source Type: Journal    
DOI: 10.1016/0921-8777(95)00063-1     Document Type: Article

References (10)

1. Ahnström, G. and K. Erixon (1981) Measurement of strand breaks by alkaline denaturation and hydroxylapatite chromatography, in: E.C. Friedberg and P.C. Hanawalt (Eds.), DNA Repair: A Laboratory Manual of Research Procedures, Marcel Dekker, Inc., New York and Basel, pp. 403-418.
2. Fairbairn, D.W., P.L. Olive and K.L. O'Neill (1995) The comet assay: a comprehensive review, Mutation Res., 339, 37-59.
3. Hjertvik, M. and G. Ahnström, in preparation.
4. Jackson, D.A. and P.R. Cook (1986) Replication occurs at a nucleoskeleton, EMBO J., 5, 1403-1410.
5. Ljungman, M., S. Nyberg, J. Nygren, M. Eriksson and G. Ahnström (1991) DNA-bound proteins contribute much more than soluble intracellular compounds to the intrinsic protection against radiation-induced DNA strand breaks in human cells, Rad. Res., 127, 171-176.
6. McKelvey-Martin, V.J., M.H.L Green, P. Schmezer, B.L. PoolZobel, M.P. De Méo and A. Collins (1993) The single cell gel electrophoresis (comet assay): a European review, Mutation Res., 288, 47-63.
7. Olive, P.L., D. Wlodek, R.E. Durand and J.P. Banáth (1992) Factors influencing DNA migration from individual cells subjected to gel electrophoresis, Exptl. Cell Res., 198, 259-267.
8. Östling, O. and K.J. Johanson (1984) Microelectrophoretic study of radiation-induced DNA damages in individual cells, Biochem. Biophys. Res. Commun., 123, 291-298.
9. Östling, O. and K.J. Johanson (1987) Bleomycin, in contrast to gamma irradiation, induces extreme variation of DNA strand breakage from cell to cell, Int. J. Radiat. Biol., 52, 683-691.
10. Singh, N.P., M.T. McCoy, R.R. Tice, and E.L. Schneider (1988) A simple technique for quantification of low levels of DNA damage in individual cells, Exp. Cell Res., 17, 184-191.