Kinetics of DNA strand breaks and protection by antioxidants in UVA- or UVB-irradiated HaCaT keratinocytes using the single cell gel electrophoresis assay

Mutation Research - DNA Repair

Volumn 407, Issue 2, 1998, Pages 97-108

  Lehmann, Jutta ^a   Pollet, Dieter ^a   Peker, Stefan ^b   Steinkraus, Volker ^b   Hoppe, Udo ^a  

^a BEIERSDORF AG   (Germany)

^b UNIVERSITY OF HAMBURG   (Germany)

Author keywords

Antioxidant; Comet assay; DNA damage; DNA repair; Keratinocyte; SCGE assay; UVA; UVB

Indexed keywords

ALPHA TOCOPHEROL; ANTIOXIDANT; BUTHIONINE SULFOXIMINE;

ARTICLE; CELL CULTURE; CELL VIABILITY; CONTROLLED STUDY; DNA DAMAGE; DNA REPAIR; DNA STRAND BREAKAGE; EXCISION REPAIR; HUMAN; HUMAN CELL; IN VITRO STUDY; KERATINOCYTE; KINETICS; PRIORITY JOURNAL; RADIATION DOSE; ULTRAVIOLET A RADIATION; ULTRAVIOLET B RADIATION; ULTRAVIOLET RADIATION;

ANTIOXIDANTS; ASCORBIC ACID; BUTHIONINE SULFOXIMINE; CELL LINE; CELL SURVIVAL; DNA; DNA DAMAGE; DNA REPAIR; DOSE-RESPONSE RELATIONSHIP, RADIATION; ELECTROPHORESIS, AGAR GEL; HUMANS; KERATINOCYTES; KINETICS; RADIATION-PROTECTIVE AGENTS; TIME FACTORS; ULTRAVIOLET RAYS; VITAMIN E;

EID: 0031776788     PISSN: 09218777     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0921-8777(97)00064-5     Document Type: Article

References (36)

1. J. Moan, A. Dahlback, Ultraviolet radiation and skin cancer. Epidemiological data from Scandinavia, in: A.R. Young, L.O. Bjorn, J. Moan, W. Nultsch (Eds.), Environmental UV Photobiology, Plenum, New York, 1993.
2. C. Robert, B. Muel, A. Benoit, L. Dubertret, A. Sarasin, A. Stary, Cell survival and shuttle vector mutagenesis induced by ultraviolet A and ultraviolet B radiation in a human cell line, J. Invest. Dermatol. 106 (1996) 721-728.
3. M.A. Pathak, Ultraviolet radiation and the development of non-melanoma and melanoma skin cancer: clinical and experimental evidence, Skin Pharmacol. 4 (1991) 85-94, Suppl. 1.
4. M.L. Cunningham, N.I. Krinsky, S.M. Giovanazzi, M.J. Peak, Superoxide anion is generated from cellular metabolites by solar radiation and its components, J. Free Radic. Biol. Med. 1 (1985) 381-386.
5. M.E. Churchill, J.G. Peak, M.J. Peak, Correlation between cell survival and DNA single-strand break repair proficiency in the Chinese hamster ovary cell lines AA8 and EM9 irradiated with 365-nm ultraviolet-A radiation, Photochem. Photobiol. 53 (1991) 229-236.
6. X. Zhang, B.S. Rosenstein, Y. Wang, M. Lebwohl, D.M. Mitchell, H. Wei, Induction of 8-oxo-7,8-dihydro-2′-deoxyguanosine by ultraviolet radiation in calf thymus DNA and HeLa cells, Photochem. Photobiol. 65 (1997) 119-124.
7. B.S. Rosenstein, The induction of DNA strand breaks in normal human skin fibroblasts exposed to solar ultraviolet radiation, Radiat. Res. 116 (1988) 313-319.
8. B.S. Rosenstein, D.L. Mitchell, Action spectra for the induction of pyrimidine photoproducts and cyclobutane pyrimidine dimers in normal human skin fibroblasts, Photochem. Photobiol. 45 (1987) 775-780.
9. A. Sancar, M.-S. Tang, Nucleotide excision repair, Photochem. Photobiol. 57 (1993) 905-921.
10. N.P. Singh, M.T. Mc Coy, R.R. Tice, E.L. Schneider, A simple technique for quantitation of low levels of DNA damage in individual cells, Exp. Cell Res. 175 (1988) 184-191.
11. C.F. Arlett, J.E. Lowe, S.A. Harcourt, A.P.W. Waugh, J. Cole, L. Roza, B.L. Diffey, T. Mori, O. Nikaido, M.H.L. Green, Hypersensitivity of human lymphocytes to UV-B and solar irradiation, Cancer Res. 53 (1993) 609-614.
12. A. Hartmann, G. Speit, Genotoxic effects of chemicals in the single cell gel (SCG) test with human blood cells in relation to the induction of sister-chromatid exchanges (SCE), Mutat. Res. 346 (1995) 49-56.
13. B.L. Pool-Zobel, C. Guigas, R. Klein, C. Neudecker, H.W. Renner, P. Schmezer, Assessment of genotoxic effects by lindane, Fd. Chem. Toxic 31 (1993) 271-283.
14. M.H.L. Green, J.E. Lowe, S.A. Harcourt, P. Akinluyi, T. Rowe, J. Cole, A.V. Anstey, C.F. Arlett, UV-C sensitivity of unstimulated and stimulated human lymphocytes from normal and xeroderma pigmentosum donors in the comet assay: a potential diagnostic technique, Mutat. Res. 273 (1992) 137-144.
15. M.H.L. Green, A.P.W. Waugh, J.E. Lowe, S.A. Harcourt, J. Cole, C.F. Arlett, Effect of desoxyribonucleosides on the hypersensitivity of human peripheral blood lymphocytes to UV-B and UV-C irradiation, Mutat. Res. 315 (1994) 25-32.
16. M.H. Lankinen, L.M. Vilpo, J.A. Vilpo, UV- and γ-irradiation-induced DNA single-strand breaks and their repair in human blood granulocytes and lymphocytes, Mutat. Res. 352 (1996) 31-38.
17. K.C. Noz, M. Bauwens, P.P.W. van Buul, H. Vrolijk, A.A. Schothorst, S. Pavel, H.J. Tanke, B.J. Vermeer, Comet assay demonstrates a higher ultraviolet B sensitivity to DNA damage in dysplastic nevus cells than in common melanocytic nevus cells and foreskin melanocytes, J. Invest. Dermatol. 106 (1996) 1198-1202.
18. C.M. Gedik, S.W.B. Ewen, A.R. Collins, Single cell gel electrophoresis applied to the analysis of UV-C damage and its repair in human cells, Int. J. Radiat. Biol. 62 (1992) 313-320.
19. V.J. McKelvey, R.R. Tice, N.P. Singh, A technique for measuring DNA single strand breaks and alkali-labile sites in individual cells, Br. J. Cancer 62 (1990) 529-530.
20. P. Boukamp, R.T. Petrussevska, D. Breitkreutz, J. Hornung, A. Markham, N.B. Fusenig, Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line, J. Cell Biol. 106 (1988) 761-771.
21. E. Borenfreund, J.A. Puerner, Toxicity determined in vitro by morphological alterations and neutral red absorption, Toxicol. Lett. 24 (1985) 119-124.
22. L. Roza, G.P. van der Schans, P.H.M. Lohman, The induction and repair of DNA damage and its influence on cell death in primary human fibroblasts exposed to UVA or UVC irradiation, Mutat. Res. 146 (1985) 89-98.
23. E.K. Henriksen, J. Moan, O. Kaalhus, G. Brunborg, Induction and repair of DNA damage in UV-irradiated human lymphocytes. Spectral differences and repair kinetics, J. Photochem. Photobiol. B Biol. 32 (1995) 39-48.
24. P. Fortini, G. Raspaglio, M. Falchi, E. Dogliotti, Analysis of DNA alkylation damage and repair in mammalian cells by the comet assay, Mutagenesis 11 (1996) 169-175.
25. T.A. Lehman, R. Modali, P. Boukamp, J. Stanek, W.P. Bennett, J.A. Welsh, R.A. Metcalf, M.R. Stampfer, N. Fusenig, E.M. Rogan, C.C. Harris, p53 mutations in human immortalized epithelial cell lines, Carcinogenesis 14 (1993) 833-839.
26. M.B. Kastan, Q. Zhan, W.S. el-Deiry, F. Carrier, T. Jacks, W.V. Walsh, B.S. Plunkett, B. Vogelstein, A.J. Fornace Jr., A mammalian cell cycle checkpoint pathway, Cell 71 (1992) 587-597.
27. S.W. Lowe, E.M. Schmitt, S.W. Smith, B.A. Osborne, T. Jacks, p53 is required for radiation-induced apoptosis in mouse thymocytes, Nature 362 (1993) 847-849.
28. G. Li, V.C. Ho, D.L. Mitchell, M.J. Trotter, V.A. Tron, Differentiation-dependent p53 regulation of nucleotide excision repair in keratinocytes, AJP 150 (1997) 1457-1464.
29. A.S. Jonason, S. Kunala, G.J. Price, R.J. Restifo, H.M. Spinelli, J.A. Persing, D.J. Leffell, R.E. Tarone, D.E. Brash, Frequent clones of p53-mutated keratinocytes in normal human skin, Proc. Natl. Acad. Sci. 93 (1996) 14025-14029.
30. A. de Laat, M. van Tilburg, J.C. van der Leun, W.A. van Vloten, F.R. de Gruijl, Cell cycle kinetics following UVA irradiation in comparison to UVB and UVC irradiation, Photochem. Photobiol. 63 (1996) 492-497.
31. W.K. Kaufmann, J.E. Cleaver, R.B. Painter, Ultraviolet radiation inhibits replicon initiation in S phase human cells, Biochim. Biophys. Acta 608 (1980) 191-195.
32. P.J. Smith, M.C. Paterson, Lethality and the induction and repair of DNA damage in far, mid or near UV-irradiated human fibroblasts: comparison of effects in normal, xeroderma pigmentosum and Bloom's syndrome cells, Photochem. Photobiol. 36 (1982) 333-343.
33. K. Sato, K. Yoshikawa, Simple system to irradiate cultured cells with UVA light, Acta Derm.-Venereol. 72 (1992) 98-101.
34. L.A. Applegate, D. Lautier, E. Frenk, R.M. Tyrrell, Endogenous glutathione levels modulate the frequency of both spontaneous and long wavelength ultraviolet induced mutations in human cells, Carcinogenesis 13 (1992) 1557-1560.
35. S. Kobayashi, M. Takehana, S. Itoh, E. Ogata, Protective effect of Mg-L-ascorbyl-2 phosphate against skin damage induced by UVB irradiation, Photochem. Photobiol. 64 (1996) 224-228.
36. D. Anderson, T.-W. Yu, B.J. Phillips, P. Schmezer, The effect of various antioxidants and other modifying agents on oxygen-radical-generated DNA damage in human lymphocytes in the comet assay, Mutat. Res. 307 (1994) 261-271.