The N-terminal portion of the preToc75 transit peptide interacts with membrane lipids and inhibits binding and import of precursor proteins into isolated chloroplasts

European Journal of Biochemistry

Volumn 268, Issue 14, 2001, Pages 4036-4043

  Inoue, Kentaro ^a   Demel, Rudy ^b   De Kruijff, Ben ^b   Keegstra, Kenneth ^a  

^a MICHIGAN STATE UNIVERSITY   (United States)

^b UTRECHT UNIVERSITY   (Netherlands)

Author keywords

Chloroplast protein import; IMPACT system; Peptide lipid interaction; Toc75; Transit peptide

Indexed keywords

DIACYLGLYCEROL DERIVATIVE; DIGALACTOSYL DIACYLGLYCEROL; INTEIN; MEMBRANE LIPID; MONOGALACTOSYL DIACYLGLYCEROL; OUTER MEMBRANE PROTEIN; PEPTIDE; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLETHANOLAMINE; PHOSPHATIDYLGLYCEROL; PROTEIN PRECURSOR; PROTEIN SUBUNIT; PROTEIN TOC75; RIBULOSEBISPHOSPHATE CARBOXYLASE; SULFOQUINOVOSYL DIACYLGLYCEROL; UNCLASSIFIED DRUG;

AMINO ACID ANALYSIS; AMINO TERMINAL SEQUENCE; ARTICLE; CHLOROPLAST; COMPETITIVE INHIBITION; ENZYME SUBUNIT; ESCHERICHIA COLI; EXPRESSION VECTOR; ISOTOPE LABELING; LIPID MONOLAYER; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN LIPID INTERACTION; PROTEIN PURIFICATION; PROTEIN TARGETING; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

CHLOROPLASTS; MEMBRANE PROTEINS; PEAS; PEPTIDE FRAGMENTS; PLANT PROTEINS; PROTEIN BINDING; PROTEIN PRECURSORS; PROTEIN SORTING SIGNALS; PROTEIN TRANSPORT; RIBULOSE-BISPHOSPHATE CARBOXYLASE;

EID: 0034827035     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2001.02316.x     Document Type: Article

References (30)

1. Protein import and routing systems of chloroplasts
2. Import and routing of nucleus-encoded chloroplast proteins
3. Domain structure of mitochondrial and chloroplast targeting peptides
4. Chloroplast transit peptides: Structure, function and evolution
5. Two components of the chloroplast protein import apparatus, IAP86 and IAP75, interact with the transit sequence during the recognition and translocation of precursor proteins at the outer envelope
6. Secondary structure and folding of a functional chloroplast precursor protein
7. The transit sequence mediates the specific interaction of the precursor of ferredoxin with chloroplast envelope membrane lipids
8. Functional domains of the ferredoxin transit sequence involved in chloroplast import
9. The structural flexibility of the preferredoxin transit peptide
10. Expression, isolation, and characterization of a chloroplast targeting peptide
11. Structure, dynamics, and insertion of a chloroplast targeting peptide in mixed micelles
12. Lipid-peptide interactions between fragments of the transit peptide of ribulose-1,5-bisphosphate carboxylase/oxygenase and chloroplast membrane lipids
13. Synthetic analogues of a transit peptide inhibit binding or translocation of chloroplastic precursor proteins
14. In vitro interaction between a chloroplast transit peptide and chloroplast outer envelope lipids is sequence-specific and lipid class-dependent
15. The C-terminus of a chloroplast precursor modulates its interaction with the translocation apparatus and PIRAC
16. In vivo and in vitro interaction of DnaK and a chloroplast transit peptide
17. Identification of a Hsp70 recognition domain within the rubisco small subunit transit peptide
18. Characterization of the import process of a transit peptide into chloroplasts
19. Isolation of components of the chloroplast protein import machinery
20. A component of the chloroplastic protein import apparatus is targeted to the outer envelope membrane via a novel pathway
21. Reconstitution of a chloroplast protein import channel
22. Analysis of the interactions of preproteins with the import machinery over the course of protein import into chloroplasts
23. A novel, bipartite transit peptide targets OEP75 to the outer membrane of the chloroplastic envelope
24. Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element
25. Investigation of the bicinchoninic acid protein assay: Identification of the groups responsible for color formation
26. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa
27. Envelope membrane proteins that interact with chloroplastic precursor proteins
28. In vitro import of proteins into chloroplasts
29. Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid
30. Biophysical characterization of a transit peptide directing chloroplast protein import