Kinetics of HCMV immediate early mRNA expression in stably transfected fibroblasts

Journal of Cell Science

Volumn 115, Issue 2, 2002, Pages 321-328

  Snaar, Sabine P ^a   Verdijk, Pauline ^a   Tanke, Hans J ^a   Dirks, Roeland W ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Confocal scanning laser microscopy; Fluorescent in situ hybridization; Human cytomegalovirus; RNA polymerase II

Indexed keywords

IMMEDIATE EARLY PROTEIN; MESSENGER RNA PRECURSOR; NUCLEAR RNA; RNA POLYMERASE II; VIRUS MESSENGER RNA;

ANIMAL CELL; ARTICLE; CELL NUCLEUS; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME INHIBITION; FIBROBLAST; FLUORESCENCE IN SITU HYBRIDIZATION; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; HUMAN CYTOMEGALOVIRUS; IMMEDIATE EARLY GENE; MICROBIAL KINETICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; RAT; RNA SPLICING; TEMPERATURE DEPENDENCE; TIME; VIRUS EXPRESSION;

ACTIVE TRANSPORT, CELL NUCLEUS; ANIMALS; CELL NUCLEUS; CELLS, CULTURED; CYTOMEGALOVIRUS; GENE EXPRESSION REGULATION; GENETIC VECTORS; HUMANS; KINETICS; NUCLEIC ACID SYNTHESIS INHIBITORS; RATS; RNA POLYMERASE II; RNA SPLICING; RNA, MESSENGER; TEMPERATURE; TIME FACTORS; TRANSCRIPTION, GENETIC;

ANIMALIA; CYTOMEGALOVIRUS; DNA VIRUSES; EUKARYOTA; HUMAN HERPESVIRUS 5;

EID: 0037081567     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (63)

1. Coupling RNA polymerase II transcription with pre-mRNA processing
2. Establishment of a rat cell line inducible for the expression of human cytomegalovirus immediate early gene products by protein synthesis inhibitors
3. Induction of gene expression under human cytomegalovirus immediate early enhancer-promoter control by inhibition of protein synthesis is cell cycle-dependent
4. Transcription-dependent redistribution of the large subunit of RNA polymerase II to discrete nuclear domains
5. Localization of poly(A)-binding protein 2 (PABP2) in nuclear speckles is independent of import into the nucleus and requires binding to poly(A) RNA
6. Regulation by HIV Rev depends upon recognition of splice sites
7. Requirement of the RNA helicase-like protein PRP22 for release of messenger RNA from spliceosomes
8. Inefficient processing impears release of RNA from the site of transcription
9. Reversible phosphorylation of the C-terminal domain of RNA polymerase II
10. Export of mRNA from microinjected nuclei of Xenopus laevis oocytes
11. Cell-cycle-dependent gene expression studied by two-colour fluorescent detection of a mRNA and histone mRNA
12. Methodologies for specific intron and exon RNA localization in cultured cells by haptenized and fluorochromized probes
13. Splicing factors associate with nuclear HCMV-IE transcripts after transcriptional activation of the gene, but dissociate upon transcription inhibition: Evidence for a dynamic organization of splicing factors
14. Quantitative imaging of pre-mRNA splicing factors in living cells
15. Quantitative digital analysis of diffuse and concentrated nuclear distributions of nascent transcripts, SC35 and poly(A)
16. Visualization of single RNA transcripts in situ
17. Capping, splicing, and 3′ processing are independently stimulated by RNA polymerase II: Different functions for different segments of the CTD
18. Assembly of the nuclear transcription and processing machinery: Cajal bodies (coiled bodies) and transcriptosomes
19. Transport between the cell nucleus and the cytoplasm
20. Selection and nuclear immobilization of exportable RNAs
21. TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus
22. RNA polymerase II and the integration of nuclear events
23. Splicing factors SR20 and 9G8 promote the nucleocytoplasmic export of mRNA
24. Numbers and organization of RNA polymerases, nascent transcripts, and transcription units in HeLa nuclei
25. The balance sheet for transcription: An analysis of nuclear RNA metabolism in mammalian cells
26. Nuclear export of different classes of RNA is mediated by specific factors
27. A block to mRNA nuclear export in S. cerevisiae leads to hyperadenylation of transcripts that accumulate at the site of transcription
28. Tracking COL1A1 RNA in osteogenesis imperfecta: Splice-defective transcripts initiate transport from the gene but are retained within the SC35 domain
29. The human Tap protein is a nuclear mRNA export factor that contains novel RNA-binding and nucleocytoplasmic transport sequences
30. The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human
31. Different phosphorylated forms of RNA polymerase II and associated mRNA processing factors during transcription
32. Highly localized tracks of specific transcripts within interphase nuclei visualized by in situ hybridization
33. The exon-exon junction complex provides a binding olatform for factors involved in mRNA export and nonsense-mediated mRNA decay
34. Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm
35. Messenger RNAs are recruited for nuclear export during transcription
36. Splicing is required for rapid and efficient mRMA export in metazoans
37. Monitoring morphology and signal during non-radioactive in situ hybridization procedures by reflection-contrast microscopy and transmission electron microscopy
38. Evaluation of pepsin treatment for electron microscopic RNA in situ hybridization on ultra-thin cryosections of cultured cells
39. Nuclear pre-mRNA compartmentalization: Trafficking of released transcripts to splicing factor reservoirs
40. Prespliceosomal assembly on microinjected precursor mRNA takes place in nuclear speckles
41. Electron tomography reveals posttranscriptional binding of pre-mRNPs to specific fibers in the nucleoplasm
42. The dynamics of a pre-mRNA splicing factor in living cells
43. The cellular organization of gene expression
44. RNA polymerase II targets pre-mRNA splicing factors to transcription sites in vivo
45. A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix
46. A human RNA helicase-like protein. HRH1, facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome
47. Intracellular dynamics of c-myc mRNA traffic in single cells in situ
48. Inhibition of RNA synthesis by actinomycin D: Characteristic dose-response of different RNA species
49. Intranuclear diffusion and hybridization state of oligonucleotides measured by fluorescence correlation spectroscopy in living cells
50. Movement of nuclear poly(A) RNA throughout the interchromatin space in living cells
51. Bicolor fluorescence in situ hybridization to intron and exon mRNA sequences
52. Dynamic association of capping enzymes with transcribing RNA polymerase II
53. RNA helicase dynamics in pre-mRNA splicing
54. The intranuclear movement of Balbiani ring premessenger ribonucleoprotein particles
55. RNA polymerase II localizes at sites of human cytomegalovirus immediate-early RNA synthesis and processing
56. Nuclear organization of pre-mRNA processing
57. TAPping into transport
58. The human dystrophin gene requires 16 hours to be transcribed and is cotranscriptionally spliced
59. Fluorescence in situ hybridization using horseradish peroxidase labeled oligonucleotides and tyramide signal amplification for sensitive DNA and mRNA detection
60. A nuclear cap-binding complex binds Balbiani ring pre-mRNA cotranscriptionally and accompanies the ribonucleoprotein particle during nuclear export
61. Nonrandom gene organization: Structural arrangements of specific pre-mRNA transcription and splicing with SC-35 domains
62. The transcriptional elongation inhibitor 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole inhibits transcription factor IIH-associated protein kinase
63. Participation of the C-terminal domain of RNA polymerase II in exon definition during pre-mRNA splicing