The dynamics of a pre-mRNA splicing factor in living cells

Nature

Volumn 387, Issue 6632, 1997, Pages 523-527

  Misteli, Tom ^a   Cáceres, Javier F ^a   Spector, David L ^a  

^a Howard Hughes Medical Institute Cold Spring Harbor Laboratory Cold Spring Harbor   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

OKADAIC ACID;

ARTICLE; GENE ACTIVATION; GENETIC TRANSCRIPTION; INTERPHASE; PRIORITY JOURNAL; PROTEIN ANALYSIS; RNA SPLICING; TRANSCRIPTION INITIATION;

CELL COMPARTMENTATION; CELL LINE; CELL NUCLEUS; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; NUCLEAR PROTEINS; PHOSPHORYLATION; RECOMBINANT FUSION PROTEINS; RNA POLYMERASE II; RNA PRECURSORS; RNA SPLICING; TRANSCRIPTION, GENETIC;

EID: 1842376906     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/387523a0     Document Type: Article

References (29)

1. Beyer, A. L. & Osheim, Y. N. Splice site selection, rate of splicing and alternative splicing on nascent transcripts. Genes Dev. 2, 754-765 (1988).
2. Moore, J. M., Query, C. C. & Sharp, P. A. in The RNA World (eds Gesteland, R. F. & Atkins, J. F.) 303-358 (Cold Spring Harbor Laboratory Press, New York, 1993).
3. Spector, D. L. Macromolecular domains within the cell nucleus. Annu. Rev. Cell Biol. 9, 265-315 (1993).
4. Prasher, D. C., Eckenrode, C. K., Ward, W. W., Prendergast, F. G. & Cormier, M. J. Primary structure of the Aequorea victoria green-fluorescent protein. Gene 111, 229-233 (1992).
5. Ge, H., Zuo, P. & Manley, J. L. Primary structure of the human splicing factor ASF reveals similarities with Drosophila regulators. Cell 66, 373-382 (1991).
6. Krainer, A. R., Mayeda, A., Kozak, D. & Binns, G. Functional expression of cloned human splicing factor SF2: Homology to RNA-binding protein, U1-70K, and Drosophila splicing regulators. Cell 66, 383-394 (1991).
7. Cáceres, J. F., Stamm, S., Helfman, D. M. & Krainer, A. R. Regulation of alternative splicing in vivo by overexpression of antagonistic splicing factors. Science 265, 1706-1709 (1994).
8. Jiménez-García, L. F. & Spector, D. L. In vivo evidence that transcription and splicing are coordinated by a recruiting mechanism. Cell 73, 47-59 (1993).
9. Huang, S. & Spector, D. L. Intron-dependent recruitment of pre-mRNA splicing factors to sites of transcription. J. Cell Biol. 131, 719-732 (1996).
10. Helfman, D. M., Ricci, W. M. & Finn, L. A. Alternative splicing of tropomyosin pre-mRNAs in vitro and in vivo. Genes Dev. 2, 1627-1638 (1988).
11. Fu, X.-D. & Maniatis, T. Factor required for mammalian spliceosome assembly is located to discrete regions in the nucleus. Nature 343, 437-441 (1990).
12. Lindell, T. J., Weinberg, F., Morris, P. W., Roeder, R. G. & Rutter, W. J. Specific inhibition of nuclear RNA polymerase II by α-amanitin. Science 170, 447-448 (1970).
13. Gui, J. F., Lane, W. S. & Fu, X.-D. A serine kinase regulates intracellular localization of splicing factors in the cell cycle. Nature 369, 678-682 (1994).
14. Colwill, K. et al. The Clk/Sty protein kinase phosphorylates splicing factors and regulates their intranuclear distribution. EMBO J. 15, 265-275 (1996).
15. Misteli, T. & Spector, D. L. Protein phosphorylation and the nuclear organization of pre-mRNA splicing. Trends Cell Biol. 7, 135-138 (1997).
16. Gadbois, D. M., Hamaguchi, J. R., Swank, R. A. & Bradbury, E. M. Staurosporine is a potent inhibitor of p34cdc2 and p34cdc2-like kinases. Biochem. Biophys. Res. Commun. 184, 80-85 (1992).
17. Bialojan, C. & Takai, A. Inhibitory effect of a marine-sponge toxin, okadaic acid, on proton phosphatases. Biochem. J. 256, 283-290 (1988).
18. Mermoud, J. E., Cohen, P. T. W. & Lamond, A. I. Regulation of mammalian spliceosome assembly by a protein phosphorylation mechanism. EMBO J. 13, 5679-5688 (1994).
19. Misteli, T. & Spector, D. L. Serine/threonine phosphatase 1 modulates the subnuclear distribution of pre-mRNA splicing factors. Mol. Biol. Cell 7, 1559-1572 (1996).
20. Moens, U., Sundsfjord, A., Flægstad, T. & Traavik, T. BK virus early RNA transcripts in stably transformed cells: enhanced levels induced by dibutyryl cyclic AMP, forskolin and 12-O-tetradecanoylphorbol-13-acetate treatment. Gen. Virol. 71, 1461-1471 (1990).
21. Boom, R. et al. Establishment of a rat cell line inducible for the expression of human cytomegalovirus immediate early gene products by protein synthesis inhibitors. J. Virol. 58, 851-859 (1986).
22. Dirks, R. W., de Pauw, E. S. D. & Raap, A. K. Splicing factors associate with nuclear HCMV-IE transcripts after transcriptional activation of the gene, but dissociate upon transcription inhibition: evidence for a dynamic organization of splicing factors. J. Cell Sci. 110, 505-513 (1997).
23. Pombo, A., Ferreira, J., Bridge, E. & Carmo-Fonseca, M. Adenovirus replication and transcription sites are spatially separated in the nucleus of infected cells. EMBO J. 13, 5075-5085 (1994).
24. Xing, Y., Johnson, C. V., Moen, P. T., McNeil, J. A. & Lawrence, J. B. Nonrandom gene organization: Structural arrangements of specific pre-mRNA transcription and splicing with SC-35 domains. J. Cell Biol. 131, 1635-1647 (1995).
25. Fakan, S. Perichromatin fibrils are in situ forms of nascent transcripts. Trends Cell Biol. 4, 86-90 (1994).
26. Mortillaro, M. J. et al. A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix. Proc. Natl Acad. Sci. USA 93, 8253-8257 (1996).
27. Kim, E., Du, L., Bregman, D. B. & Warren, S. L. Splicing factors associate with hyperphosphorylated RNA polymerase II in the absence of pre-mRNA J. Cell Biol. 136, 19-28 (1997).
28. McCracken, S. et al. The C-terminal domain of RNA polymerase II couples mRNA processing to transcription. Nature 385, 357-361 (1997).
29. Habets, W. J., Hoet, M. H., DeJong, B. A. W., VanDer Kemp, A. & Van Venrooij, W. J. Mapping of B cell epitopes on small nuclear ribonucleoproteins that react with human autoantibodies as well as with experimentally induced mouse monoclonal antibodies. J. Immunol. 143, 2560-2466 (1986).