Messenger RNAs are recruited for nuclear export during transcription

Genes and Development

Volumn 15, Issue 14, 2001, Pages 1771-1782

  Lei, Elissa P ^a   Krebber, Heike ^a,b   Silver, Pamela A ^a  

^a DANA FARBER CANCER INSTITUTE   (United States)

^b PHILIPPS UNIVERSITY MARBURG   (Germany)

Author keywords

Chromatin IP; mRNA export; Npl3; RNA polymerase II; Transcription; Yra1

Indexed keywords

RNA POLYMERASE; TATA BINDING PROTEIN; TRANSCRIPTION FACTOR;

ANIMAL CELL; ARTICLE; CELL COMPARTMENTALIZATION; CELL NUCLEUS TRANSPLANTATION; CHROMATIN STRUCTURE; GENE MUTATION; IMMUNOPRECIPITATION; MACROMOLECULE; MESSENGER RNA SYNTHESIS; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DOMAIN; RNA SPLICING; SACCHAROMYCES CEREVISIAE; TRANSCRIPTION REGULATION;

BIOLOGICAL TRANSPORT; CELL CYCLE PROTEINS; CELL NUCLEUS; CHROMATIN; DNA-BINDING PROTEINS; FUNGAL PROTEINS; MODELS, GENETIC; MUTATION; NUCLEAR PROTEINS; RNA POLYMERASE II; RNA, FUNGAL; RNA, MESSENGER; RNA-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TATA-BOX BINDING PROTEIN; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

ANIMALIA; EUKARYOTA; SACCHAROMYCES CEREVISIAE;

EID: 0035878119     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: 10.1101/gad.892401     Document Type: Article

References (61)

1. A protein of the SR family of splicing factors binds extensively to exonic Balbiani ring premRNA and accompanies the RNA from the gene to the nuclear pore
2. Isolation and characterization of RAT1: An essential gene of Saccharomyces cerevisiae required for the efficient nucleocytoplasmic trafficking of mRNA
3. TBP mutants defective in activated transcription in vivo
4. The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates
5. A mutant nuclear protein with similarity to RNA binding proteins interferes with nuclear import in yeast
6. mRNA capping enzyme is recruited to the transcription complex by phosphorylation of the RNA polymerase II car-boxy-terminal domain
7. The TATA-binding protein is required for transcription by all three nuclear RNA polymerases in yeast cells
8. Transcription factor TFIID recruits factor CPSF for formation of 3′ end of mRNA
9. Enhancement of mRNA nuclear transport by promoter elements
10. The Spt components of SAGA facilitate TBP binding to a promoter at a post-activator-binding step in vivo
11. Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking
12. A yeast RNA-binding protein shuttles between the nucleus and the cytoplasm
13. Phosphorylation by Skylp promotes Npl3p shuttling and mRNA dissociation
14. The yeast hnRNP-like protein Hrp1/Nab4 marks a transcript for nonsense-mediated mRNA decay
15. TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus
16. Guide to yeast genetics and molecular biology
17. Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae
18. Potential RNA binding proteins in Saccharomyces cerevisiae identified as suppressors of temperature-sensitive mutations in NPL3
19. RNA polymerase II and the integration of nuclear events
20. Rat8p/Dbp5p is a shuttling transport factor that interacts with Rat7p/Nup159p and Gle1p and suppresses the mRNA export defect of xpol-1 cells
21. Active disruption of an RNA-protein interaction by a DExH/D RNA helicase
22. In vitro transcription from the adenovirus 2 major late promoter utilizing templates truncated at promoter-proximal sites
23. Nuclear mRNA accumulation causes nucleolar fragmentation in yeast mtr2 mutant
24. The human Tap protein is a nuclear mRNA export factor that contains novel RNA-binding and nucleocytoplasmic transport sequences
25. The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human
26. Pre-mRNA splicing imprints mRNA in the nucleus with a novel RNA-binding protein that persists in the cytoplasm
27. Hrpl, a sequence-specific RNA-binding protein that shuttles between the nucleus and the cytoplasm, is required for mRNA 3′ -end formation in yeast
28. Different phosphorylated forms of RNA polymerase II and associated mRNA processing factors during transcription
29. Uncoupling of the hnRNP Npl3p from mRNAs during the stress-induced block in mRNA export
30. Genetic analysis of the large subunit of yeast transcription factor IIE reveals two regions with distinct functions
31. Binding of TBP to promoters in vivo is stimulated by activators and requires Pol II holoenzyme
32. Classical mutagenesis techniques
33. The spliceosome deposits multiple proteins nucleotides upstream of mRNA exon-exon junctions
34. A protein that shuttles between the nucleus and the cytoplasm is an important mediator of RNA export
35. Splicing is required for rapid and efficient mRNA export in metazoans
36. The C-terminal domain of RNA polymerase II couples mRNA processing to transcription
37. A hyper-phosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix
38. Analysis of chromatin structure by in vivo formaldehyde cross-linking
39. A distinct and parallel pathway for the nuclear import of an mRNA-binding protein
40. In vivo transcriptional pausing and cap formation on three Drosophila heat shock genes
41. REF proteins mediate the export of spliced and unspliced mRNAs from the nucleus
42. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector
43. The addition of 5′ cap structures occurs early in hnRNA synthesis and prematurely terminated molecules are capped
44. Nuclear mRNA export requires complex formation between Mex67p and Mtr2p at the nuclear pores
45. Dynamic association of capping enzymes with transcribing RNA polymerase II
46. Mtr10p functions as a nuclear import receptor for the mRNA-binding protein Npl3p
47. Arginine methylation facilitates the nuclear export of hnRNP proteins
48. The yeast mRNA-binding protein Npl3p interacts with the cap-binding complex
49. Characterization of the ptr6(+) gene in fission yeast: A possible involvement of a transcriptional coactivator TAF in nucleocytoplasmic transport of mRNA
50. A yeast protein that bidirectionally affects nucleocytoplasmic transport
51. Dbp5p/Rat8p is a yeast nuclear pore-associated DEAD-box protein essential for RNA export
52. The RNA export factor Glelp is located on the cytoplasmic fibrils of the NPC and physically interacts with the FG-nucleoporin Rip1p, the DEAD-box protein Rat8p/DbpSp and a new protein Ymr255p
53. Yralp, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export
54. Binding of the Mex67p/Mtr2p heterodimer to FXFG, GLFG, and FG repeat nucleoporins is essential for nuclear mRNA export
55. The hrp23 protein in the balbiani ring pre-mRNP particles is released just before or at the binding of the particles to the nuclear pore complex
56. General requirement for RNA polymerase II holoenzymes in vivo
57. A pre-mRNA-binding protein accompanies the RNA from the gene through the nuclear pores and into polysomes
58. A nuclear cap-binding complex binds Balbiani ring pre-mRNA cotranscriptionally and accompanies the ribonucleoprotein particle during nuclear export
59. Characterization of nuclear polyadenylated RNA-binding proteins in Saccharomyces cerevisiae
60. The C-terminal domain of the largest subunit of RNA polymerase II interacts with a novel set of serine/arginine-rich proteins
61. The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans