Capping, splicing, and 3′ processing are independently stimulated by RNA polymerase II: Different functions for different segments of the CTD

Genes and Development

Volumn 15, Issue 14, 2001, Pages 1783-1795

  Fong, N ^a   Bentley, D L ^a  

^a UNIVERSITY OF COLORADO   (United States)

Author keywords

Carboxy terminal domain; CstF; mRNA processing; RNA polymerase II; Splicing

Indexed keywords

RNA POLYMERASE;

ANIMAL CELL; ARTICLE; CAPPING PHENOMENON; CARBOXY TERMINAL SEQUENCE; CELL VIABILITY; COMPLEX FORMATION; GENE DISRUPTION; INTRON; NONHUMAN; POLYADENYLATION; PRIORITY JOURNAL; PROTEIN DOMAIN; RNA CLEAVAGE; RNA SPLICING; RNA TRANSCRIPTION; TANDEM REPEAT; TRANSCRIPTION REGULATION;

AMINO ACID SEQUENCE; MOLECULAR SEQUENCE DATA; MRNA CLEAVAGE AND POLYADENYLATION FACTORS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; REPETITIVE SEQUENCES, AMINO ACID; RNA CAPS; RNA POLYMERASE II; RNA PROCESSING, POST-TRANSCRIPTIONAL; RNA SPLICING; RNA-BINDING PROTEINS; SEQUENCE DELETION;

ANIMALIA;

EID: 0035878112     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: 10.1101/gad.889101     Document Type: Article

References (58)

1. Cleavage/polyadenylation factor IA associates with the carboxyl-terminal domain of RNA polymerase II in Saccharomyces cerevisiae
2. Genetic analysis of the repetitive carboxyl-terminal domain of the largest subunit of mouse RNA polymerase II
3. Transcriptional termination in the Balbiani ring 1 gene is closely coupled to 3′-end formation and excision of the 3′-terminal intron
4. Coupling RNA polymerase II transcription with pre-mRNA processing
5. Splice site selection, rate of splicing, and alternative splicing on nascent transcripts
6. Exonic splicing enhancers: Mechanism of action, diversity and role in human genetic diseases
7. mRNA capping enzyme is recruited to the transcription complex by phosphorylation of the RNA polymerase II carboxy-terminal domain
8. The cap and the 3′ splice site similarly affect polyadenylation efficiency
9. Promoter-proximal pausing by RNA polymerase II in vitro: Transcripts shorter than 20 nucleotides are not capped
10. Identification of a new class of exonic splicing enhancers by in vivo selection
11. Coupling of transcription with alternative splicing: RNA Pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer
12. Reversible phosphorylation of the C-terminal domain of RNA polymerase II
13. A human homologue of the Drosophila sex determination factor transformer-2 has conserved splicing regulatory functions
14. Functional interaction between the carboxy-terminal domain of RNA polymerase II and pre-messenger RNA splicing
15. Terminal exon definition occurs cotranscriptionally and promotes termination of RNA polymerase II
16. Participation of the nuclear cap binding complex in pre-mRNA 3′ processing
17. RNA polymerase II C-terminal domain required for enhancer-driven transcription
18. Positive patches and negative noodles: Linking RNA processing to transcription?
19. Functional mRNA can be generated by RNA polymerase III
20. Poly(A) site cleavage in a HeLa nuclear extract is dependent on downstream sequences
21. RNA polymerase II is an essential mRNA polyadenylation factor
22. RNA polymerase II and the integration of nuclear events
23. Phosphorylated RNA polymerase II stimulates pre-mRNA splicing
24. Distinct effector roles for Ser2 and Ser5 phosphorylation of the RNA polymerase II CTD in the recruitment and allosteric activation of mammalian capping enzyme
25. Effect of the cap structure on pre-mRNA splicing in Xenopus oocyte nuclei
26. Splicing factors associate with hyperphosphorylated RNA polymerase II in the absence of pre-messenger RNA
27. Functional interaction of BRCAl-associated BARD1 with polyadenylation factor CstF-50
28. Different phosphorylated forms of RNA polymerase II and associated mRNA processing factors during transcription
29. A splicing enhancer in the human fibronectin alternate ED 1 exon interacts with SR proteins and stimulates U2 snRNP binding
30. Transcription of eukaryotic protein-coding genes
31. A nuclear cap-binding complex facilitates association of U1-snRNP with the cap-proximal 5′ splice-site
32. Growth retardation and neonatal lethality in mice with a homozygous deletion in the C-terminal domain of RNA polymerase II
33. 5′-capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II
34. The C-terminal domain of RNA polymerase II couples messenger RNA processing to transcription
35. Role of RNA polymerase II carboxy-terminal domain in coordinating transcription with RNA processing
36. RNA polymerase II targets pre-mRNA splicing factors to transcription sites in vivo
37. A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix
38. In vivo degradation of RNA polymerase II largest subunit triggered by alphaamanitin
39. Mutation of the AAUAAA polyadenylation signal depresses in vitro splicing of proximal but not distal introns
40. In vitro polyadenylation is stimulated by the presence of an upstream intron
41. Growth-related changes in phosphorylation of yeast RNA polymerase II
42. A subset of SR proteins activates splicing of the cardiac troponin T alternative exon by direct interactions with an exonic enhancer
43. In-vivo transcriptional pausing and cap formation on three Drosophila heat-shock genes
44. Cyclin C/CDK8 and cyclin H/CDK7/p36 are biochemically distinct CTD kinases
45. Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II
46. Dynamic association of capping enzymes with transcribing RNA polymerase II
47. Transcription of herpes simplex virus tk sequences under the control of wild-type and mutant human RNA polymerase I promoters
48. Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing
49. A human polyadenylation factor is a G protein beta-subunit homologue
50. Complex protein interactions within the human polyadenylation machinery identify a novel component
51. The carboxyl terminus of vertebrate poly(A) polymerase interacts with U2AF 65 to couple 3′-end processing and splicing
52. The nuclear matrix protein p255 is a highly phosphorylated form of RNA polymerase II largest subunit which associates with spliceosomes
53. Transcriptional activation by the acidic domain of VMW65 requires the integrity of the domain and involves additional determinants distinct from those necessary for TFIIB binding
54. Construction and analysis of yeast RNA polymerase II CTD deletion and substitution mutations
55. MCM proteins are associated with RNA polymerase II holoenzyme
56. Mammalian capping enzyme complements mutant Saccharomyces cerevisiae lacking mRNA guanylyltransferase and selectively binds the elongating form of RNA Pol II
57. The CTD of RNA polymerase II interacts with a novel set of SR-like proteins
58. Participation of the C-terminal domain of RNA polymerase II in exon definition during pre-mRNA splicing