RNA polymerase II localizes at sites of human cytomegalovirus immediate- early RNA synthesis and processing

Journal of Histochemistry and Cytochemistry

Volumn 47, Issue 2, 1999, Pages 245-254

  Snaar, Sabine P ^a   Vincent, Michel ^b   Dirks, Roeland W ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^b UNIVERSITÉ LAVAL   (Canada)

Author keywords

Immunocytochemistry; In situ hybridization; Monoclonal antibody CC 3; Splicing; Transcription

Indexed keywords

RNA; RNA POLYMERASE II;

ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELLULAR DISTRIBUTION; ENZYME BINDING; GENE CLUSTER; HUMAN CYTOMEGALOVIRUS; IMMEDIATE EARLY GENE; IMMUNOCYTOCHEMISTRY; IN SITU HYBRIDIZATION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; RAT; RNA PROCESSING; RNA SPLICING; RNA SYNTHESIS; TRANSCRIPTION INITIATION;

ANIMALIA; EUKARYOTA; HUMAN HERPESVIRUS 5;

EID: 0032920756     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215549904700213     Document Type: Article

References (54)

1. Baurén G, Jiang W-Q, Bernholm K, Gu F, Wieslander L (1996) Demonstration of a dynamic, transcription-dependent organization of pre-mRNA splicing factors in polytene nuclei. J Cell Biol 133:929-941
2. Baurén G, Wieslander L (1994) Splicing of Balbiani ring 1 gene premRNA occurs simultaneously with transcription. Cell 76:183-192
3. Beyer AL, Osheim YN (1988) Splice site selection, rate of splicing, and alternative splicing on nascent transcripts. Genes Dev 2:754-765
4. Bisotto S, Lauriault P, Duval M, Vincent M (1995) Co-localization of a high molecular mass phosphoprotein of the nuclear matrix (p255) with spliceosomes. J Cell Sci108:1873-1882
5. Boom R, Geelen JL, Sol CJ, Raap AK, Minnaar RP, Klaver BP, van der Noordana J (1986) Establishment of a rat cell line inducible for the expression of human cytomegalovirus immediate early gene products by protein synthesis inhibitors. J Virol 58:851-859
6. Bregman DB, Du L, van der Zee S, Warren SL (1995) Transcription-dependent redistribution of the large subunit of RNA polymerase II to discrete nuclear domains. J Cell Biol 129:287-298
7. Carter KC, Bowman D, Carrington W, Fogarty K, McNeil JA, Fay FS, Lawrence JB (1993) A three-dimensional view of precursor messenger RNA metabolism within the mammalian nucleus. Science 259:1330-1335
8. Carter KC, Taneja KL, Lawrence JB (1991) Discrete nuclear domains of poly(A) RNA and their relationship to the functional organization of the nucleus. J Cell Biol 115:1191-1202
9. Chabot B, Bisotto S, Vincent M (1995) The nuclear matrix phosphoprotein p255 associates with splicing complexes as part of the [U4/U6.U5] tri-snRNP particle. Nucleic Acids Res 23:3206-3213
10. Cho E-J, Takagi CR, Moore CR, Buratowski S (1997) mRNA capping enzyme is recruited to the transcription complex by phosphorylation of the RNA polyrnerase II carboxyterminal domain. Genes Dev 11:3319-3326
11. Dahmus ME (1996) Reversible phosphorylation of the C-terminal domain of RNA polymerase H. J Biol Chem 271:19009-19012
12. Dirks RW (1996) RNA molecules lighting up under the microscope. Histochcm Cell Biol 106:151-166
13. Dirks RW, Daniël KC, Raap AK (1995) RNAs radiate from gene to cytoplasm as revealed by fluorescence in situ hybridization. J Cell Sci108:2565-2572
14. Dirks RW, de Pauw ESD, Raap AK (1997) Splicing factors associate with nuclear HCMV-IE transcripts after transcriptional activation of the gene, but dissociate upon transcription inhibition: evidence for a dynamic organization of splicing factors. J Cell Sci 110:515-522
15. Dirks RW, Raap AK (1995) Cell-cycle-dependent gene expression studied by two-colour fluorescent detection of a mRNA and histonc mRNA. Histochem Cell Biol 104:391-395
16. Dirks RW, van de Rijke FM, Fujishita S, van der Ploeg M, Raap AK (1993) Methodologies for specific intron and exon localization in cultured cells by haptenized and fluorochromized probes. J Cell Sci104:1187-1197
17. Du L, Warren SL (1997) A functional interaction between the carboxy-terminal domain of RNA polymerase II and pre-mRNA splicing. J Cell Biol 136:5-18
18. Duhois M-F, Bellier S, Seo S-J, Bensaude O (1994a) Phosphorylation of the RNA polymerase II largest subunit during heat shock and inhibition of transcription in HeLa cells. J Cell Physiol 158:417-426
19. Dubois M-F, Nguyen VT, Bellier S, Bensaude O (1994b) Inhibitors of transcription such as 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole and isoquinoline sulfonamide derivatives (H-8 and H-7) promote dephosphorylation of the carboxyl-terminal domain of RNA polymerase II largest subunit. J Biol Chem 269: 13331-13336
20. Fakan S (1994) Perichromatin fibrils are in situ forms of nascent transcripts. Trends Cell Biol 4:86-90
21. Grande MA, van der Kraan I, de Jong L, van Driel R (1997) Nuclear distribution of transcription factors in relation to sites of transcription and RNA polymerase II. J Cell Sci110:1781-1791
22. Greenleaf AL (1993) Positive patches and negative noodles: linking RNA processing to transcription? Trends Biochem Sci18:117-119
23. Ishov AM, Stenberg RM, Maul GG (1997) Human cytomegalovirus immediate early interaction with host nuclear structures: definition of an immediate transcript environment. J Cell Biol 138: 5-16
24. Jackson DA, Hassan AB, Errington RJ, Cook PR (1993) Visualization of focal sites of transcription within human nuclei. EMBO J 12:1059-1065
25. Jiménez-García LF, Spector DL (1993) In vivo evidence that transcription and splicing are coordinated by a recruiting mechanism. Cell 73:47-59
26. Kim E, Du L, Bregman DB, Warren SL (1997) Splicing factors associate with hyperphosphorylated RNA polymerase II in the absence of pre-mRNA. J Cell Biol 136:19-28
27. Laybourn PJ, Dahmus ME (1990) Phosphorylation of RNA polymerase IIA occurs subsequent to interaction with the promoter and before the initiation of transcription. J Biol Chem 265: 13165-13173
28. Marshall NF, Peng J, Xie Z, Price DH (1996) Control of RNA polymerase II elongation potential by a novel carboxyl-terminal domain kinase J Biol Chem 271:27176-27183
29. McCracken S, Fong N, Rosonina E, Yankulov K, Brothers G, Siderovski D, Hessel A, Foster S, Amgen EST Program, Shuman S, Bentley DL (1997a) 5′-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II. Genes Dev 11:3306-3318
30. McCracken S, Fong N, Yankulov K, Ballantyne S, Pan G, Greenblatt J, Patterson SD, Wickens M, Bentley DL (1997b) The C-terminal domain of RNA polymerase II couples mRNA processing to transcription. Nature 385:357-361
31. Misteli T, Cáceres JF, Spector DL (1997) The dynamics of a pre-mRNA splicing factor in living cells. Nature 387:523-527
32. Misteli T, Spector DL (1996) Serine/threonine phosphatase 1 modulates the subnuclear distribution of pre-mRNA splicing factors. Mol Biol Cell 7:1559-1572
33. Moen PT, Smith KP, Lawrence JB (1995) Compartmentalization of specific pre-mRNA metabolism: an emerging view. Hum Mol Genet 4:1779-1789
34. Mortillaro MJ, Blencowe BJ, Wei X, Nakayasu H, Du L, Warren SL, Sharp PA, Berezney R (1996) A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix. Proc Natl Acad Sci USA 93:8253-8257
35. Neugebauer KM, Roth MB (1997) Distribution of pre-mRNA splicing factors at sites of RNA polymerase II transcription. Genes Dev 11:1148-1159
36. O'Keefe RT, Mayeda A, Sadowski CL, Krainer AR, Spector DL (1994) Disruption of pre-mRNA splicing in vivo results in reorganization of splicing factors. J Cell Biol 124:249-260
37. Payne JM, Dahmus ME (1993) Partial purification and characterization of two distinct protein kinases that differentially phosphorylate the carboxy-terminal domain of RNA polymerase subunit ila. J Biol Chem 268:80-87
38. Perry RP, Kelly DL (1970) Inhibition of RNA synthesis by actinomycin D: characteristic dose-response of different RNA species. J Cell Physiol 76:127-139
39. Reuter R, Appel B, Bringmann P, Rinke J, Lührmann R (1984) 5′-Terminal caps of snRNAs are reactive with antibodies specific for 2,2,7-trimethylguanosine in whole cells and nuclear matrices. Exp Cell Res 154:548-560
40. Sisodia SS, Sollner-Webb B, Cleveland DW (1987) Specificity of RNA maturation pathways: RNAs transcribed by RNA polymerase III are not substrates for splicing or polyadenylation. Mol Cell Biol 7:3602-3612
41. Spector DL (1993) Macromolecular domains within the cell nucleus. Annu Rev Cell Biol 9:265-315
42. Spector DL, O'Keefe RT, Jiménez-García LF (1993) Dynamics of transcription and pre-mRNA splicing within the mammalian cell nucleus. Cold Spring Harbor Symp Quant Biol 58:799-805
43. Steinmetz EJ (1997) Pre-mRNA processing and the CTD of RNA polymerase II: the tail that wags the dog? Cell 89:491-494
44. Thompson NE, Steinberg TH, Aronson DB, Burgess RR (1989) Inhibition of in vivo and in vitro transcription by monoclonal antibodies prepared against wheat germ RNA polymerase II that react with the heptapeptide repeat of eukaryotic RNA polymerase II. J Biol Chem 264:11511-11520
45. van de Corput MPC; Dirks RW, van Gijlswijk RPM, van de Rijke FM, Raap AK (1998) Fluorescence in situ hybridization using horseradish peroxidase labeled oligonucleotides and tyramide signal amplification tor sensitive DNA and mRNA detection. Histochem Cell Biol 110:431-437
46. Vincent M, Lauriault P, Duhois M-F, Lavoie S, Bensaude O, Chabot B (19961 The nuclear matrix protein p255 is a highly phosphorylated form of RNA polymerase II largest subunit which associates with spliceosomes. Nucleic Acids Res 24:4649-4652
47. Wansirk DG, Schul W, van der Kraan I, van Steensel B, van Driel R, de Jong L (1993) Fluorescent labeling of nascent RNA reveals transcription by RNA polymerase II in domains scattered throughout the nucleus. J Cell Biol 122:283-293
48. Weeks JR, Hardin SE, Shen J, Lee JM, Greenleaf AL (1993) Locus-specific variation in phosphorylation state of RNA polymerase II in vivo: correlations with gene activity and transcript processing. Genes Dev 7:2329-2344
49. Wuarin Y, Schibler U (1994) Physical isolation of nascent RNA chains transcribed by RNA polymerase II: evidence for cotran-scriptional splicing. Mol Cell Biol 14:7219-7225
50. Xing Y, Johnson CV, Dobner PR, Lawrence JB (1993) Higher level organization of individual gene transcription and RNA splicing. Science 259:1326-1330
51. Xing Y, Johnson CV, Moen PT, McNeil JA, Lawrence JB (1995) Nonrandom gene organization: structural arrangements of specific pre-mRNA transcription and splicing with SC-35 domains. J Cell Biol 131:1635-1647
52. Yankulov K, Yamashita K, Roy R, Egly J-M, Bentley DL (1995) The transcriptional elongation inhibitor 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole inhibits transcription factor IIH-associated protein kinase. J Biol Chem 270:23922-23925
53. Yuryev A, Patturajan M, Litingtung Y, Joshi RV, Gentile C, Gebara M, Corden JL (1996) The C-terminal domain of the largest subunit of RNA polymerase II interacts with a novel set of serine/ arginine-rich proteins. Proc Natl Acad Sci USA 93:6975-6980
54. Zeng C, Kim E, Warren SL, Berget SM (1997) Dynamic relocation of transcription and splicing factors dependent upon transcriptional activity. EMBO J 16:1401-1412