Numbers and organization of RNA polymerases, nascent transcripts, and transcription units in HeLa nuclei

Molecular Biology of the Cell

Volumn 9, Issue 6, 1998, Pages 1523-1536

  Jackson, Dean A ^a   Iborra, Francisco J ^a   Manders, Erik M M ^a   Cook, Peter R ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

RNA POLYMERASE;

ARTICLE; ENZYME LOCALIZATION; GENE STRUCTURE; GENOME; HUMAN; HUMAN CELL; PRIORITY JOURNAL; RNA STRUCTURE; RNA TRANSCRIPTION;

EID: 0031813779     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.9.6.1523     Document Type: Article

References (49)

1. Beyer, A.L., Bouton, A.M., Hodge, L.D., and Miller, O.L. (1981). Visualization of the major late R strand transcription unit of adenovirus serotype 2. J. Mol. Biol. 147, 269-295.
2. Bonner, J., Gottesfeld, J., Garrard, W., Billing, R., and Uphouse, L. (1975). Isolation of template active and inactive regions of chromatin. Methods Enzymol. 40, 97-102.
3. Bregman, D.B., Du, L., van der Zee, S., and Warren, S.L. (1995). Transcription-dependent redistribution of the large subunit of RNA polvmerase II to discrete nuclear domains. J. Cell Biol. 129, 287-298.
4. Chambon, P. (1974). RNA polymerases. In: The Enzymes, ed. P.D. Boyer, New York: Academic Press, 261-331.
5. Cox, R.F. (1976). Quantitation of elongating form A and B RNA polymerases in chick oviduct nuclei and effects of estradiol. Cell 7, 455-465.
6. Dahmus M.E. (1996). Reversible phosphorylation of the C-terminal domain of RNA polymerase II. J. Biol. Chem. 271, 19009-19012.
7. Fakan, S., Leser, G., and Martin, I.E. (1986). Immunoelectron microscope visualization of nuclear ribonucleoprotein antigens spread within transcription complexes. J. Cell Biol. 103, 1153-1157.
8. Fay, F.S., Taneja, K.L., Shenoy, S., Lifshitz, L., and Singer, R.H. (1997). Quantitative digital analysis of diffuse and concentrated nuclear distributions of nascent transcripts, SC35 and poly(A). Exp. Cell Res. 231, 27-37.
9. Geiduschek, E.P., and Tocchini-Valentini, G.P. (1988). Transcription by RNA polymerase III. Annu. Rev. Biochem. 57, 873-914.
10. Harlow, E., and Lane, D. (1988). Antibodies: A Laboratory Manual, Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
11. Hawley, D.K., and Roeder, R.G. (1987). Functional steps in transcriprional initiation and reinitiation from the major late promoter in a HeLa nuclear extract. J. Biol. Chem. 262, 3452-3461.
12. Hozák, P., Cook, P.R., Schöfer, C., Mosgöller, W., and Wachtler, F. (1994). Site of transcription of ribosomal RNA and intra-nucleolar structure in HeLa cells. J. Cell Sci. 107, 639-648.
13. Hozák, P., Hassan, A.B., Jackson, D.A., and Cook, P.R. (1993). Visualization of replication factories attached to a nucleoskeleton. Cell 73, 361-373.
14. Huang, R.C., Smith, M.M., and Reeve, A.E. (1978). Studies on gene transcription in vitro by analysis of the primary transcripts. Cold Spring Harb. Symp. Quant. Biol. 42, 589-596.
15. Iborra, F.J., Pombo, A., Jackson, D.A., and Cook, P.R. (1996a). Active RNA polymerases are localized within discrete transcription 'factories' in human nuclei. J. Cell Sci. 109, 1427-1436.
16. Iborra, F.J., Pombo, A., McManus, J., Jackson, D.A., and Cook, P.R. (1996b). The topology of transcription by immobilized polymerases. Exp. Cell Res. 229, 167-173.
17. Igo-Kemenes, T., and Zachau, H.G. (1977). Domains in chromatin structure. Cold Spring Harbor Symp. Quant. Biol. 42, 109-118.
18. Iyer, V., and Struhl, K. (1996). Absolute mRNA levels and transcriptional initiation rates in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 93, 5208-5212.
19. Jackson, D.A., Bartlett, J., and Cook, P.R. (1996). Sequences attaching loops of nuclear and mitochondrial DNA to underlying structures in human cells: the role of transcription units. Nucleic Acids Res. 24, 1212-1219.
20. Jackson, D.A., and Cook, P.R. (1985). Transcription occurs at a nucleoskeleton. EMBO J. 4, 919-925.
21. Jackson, D.A., Hassan, A.B., Errington, R.J., and Cook, P.R. (1993). Visualization of focal sites of transcription within human nuclei. EMBO J. 12, 1059-1065.
22. Jackson, D.A., McCready, S.J., and Cook, P.R. (1981). RNA is synthesised at the nuclear cage. Nature 292, 552-555.
23. Jackson, D.A., Yuan, J., and Cook, P.R. (1988). A gentle method for preparing cyto- and nucleo-skeletons and associated chromatin. J. Cell Sci. 90, 365-378.
24. Kim, E., Du, L., Bregman, D.B., and Warren, S.L. (1997). Splicing factors associate with hyperphosphorylated RNA polymerase II in the absence of pre-mRNA. J. Cell Biol. 136, 19-28.
25. Laird, C.D., and Chooi, W.Y. (1976). Morphology of transcription units in Drosophila melanogaster. Chromosoma 58, 193-218.
26. Lewin, B. (1974). Gene Expression-2: Eukaryotic Chromosomes, vol. II, London: Wiley.
27. Lewin, B. (1975). Units of transcription and translation: sequence components of heterogeneous nuclear RNA and messenger RNA. Cell 4, 77-93.
28. McKnight, S.L., and Miller, O.L. (1976). Ultrastructural patterns of RNA synthesis during early embryogenesis in Drosophila melanogaster. Cell 8, 305-319.
29. McKnight, S.L., and Miller, O.L. (1979). Post-replicative nonribosomal transcription units in D. melanogaster embryos. Cell 17, 551-563.
30. Miklos, G.L.G., and Rubin, G.M. (1996). The role of the genome project in determining gene function: insights from model organisms. Cell 86, 521-529.
31. O'Brien, T. and Lis, J.T. (1993). Rapid changes in Drosophila transcription after an instantaneous heat shock. Mol. Cell. Biol. 13, 3456-3463.
32. Osheim, Y.N., and Beyer, A.L. (1989). Electron microscopy of ribonucleoprotein complexes on nascent RNA using Miller chromatin spreading method. Methods Enzymol. 180, 481-509.
33. Parra, I., and Windle, B. (1993). High resolution visual mapping of stretched DNA by fluorescent hybridization. Nat. Genet. 5, 17-21.
34. Perry, R.P., and Kelley, D.E. (1970). Inhibition of RNA synthesis by actinomycin D: characteristic dose-response of different RNA species. J. Cell Physiol. 76, 127-140.
35. Puvion, E., and Moyne, G. (1978). Intranuclear migration of newly synthesized extranucleolar ribonucleoproteins. Exp. Cell Res. 115, 79-88.
36. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
37. Shaw, P.J., and Jordan, E.G. (1995). The nucleolus. Annu. Rev. Cell Dev. Biol. 11, 93-121.
38. Shermoen, A.W., and O'Farrell, P.H. (1991). Progression of the cell cycle through mitosis leads to abortion of nascent transcripts. Cell 67, 303-310.
39. Sierakowska, H., Shuklas, R.R., Dominski, Z., and Kole, R. (1989). Inhibition of pre-mRNA splicing by 5-fluoro-, 5-chloro-, and 5-bromouridine. J. Biol. Chem. 264, 19185-19191.
40. Sogo, J.M., and Thoma, F. (1989). Electron microscopy of chromatin. Methods Enzymol. 170, 142-179.
41. Sollner-Webb, B., and Tower, J. (1986). Transcription of cloned eukaryotic ribosomal RNA genes. Annu. Rev. Biochem. 55, 801-830.
42. Sugden, B., and Keller, W. (1973). Mammalian deoxyribonucleic acid-dependent ribonucleic acid polymerases, I; purification and properties of an α-amanitin-sensitive ribonucleic acid polymerase and stimulatory factors from HeLa and KB cells. J. Biol. Chem. 248, 3777-3788.
43. Thompson, N.E., Steinberg, T.H., Aronson, D.B., and Burgess, R.R. (1989). Inhibition of in vivo and in vitro transcription by monoclonal antibodies prepared against wheat germ RNA polymerase II that react with the heptapeptide repeat of eukaryotic RNA polymerase II. J. Biol. Chem. 264, 11511-11520.
44. Wansink, D.G., Nelissen, R.L., and de Jong, L. (1994). In vitro splicing of pre-mRNA containing bromouridine. Mol. Biol. Rep. 19, 109-113.
45. Wansink, D.G., Schul, W., van der Kraan, I., van Steensel, B., van Driel, R., and de Jong, L. (1993). Fluorescent labelling of nascent RNA reveals transcription by RNA polymerase II in domains scattered throughout the nucleus. J. Cell Biol. 122, 283-293.
46. Weinmann, R., Raskas, H.J., and Roeder, R.G. (1975). The transcriptional role of host DNA-dependent RNA polymerases in adenovirus-infected KB cells. Cold Spring Harb. Symp. Quant. Biol. 34, 495-500.
47. Wijgerde, M., Grosveld, F., and Fraser, P. (1995). Transcription complex stability and chromatin dynamics in vivo. Nature 377, 209-213.
48. Wolgemuth, D.J., and Hsu, M.-T. (1981). Visualization of nascent RNA transcripts and simultaneous transcription and replication in viral nucleoprotein complexes from adenovirus 2-infected HeLa cells. J. Mol. Biol. 147, 247-268.
49. Zawel, L., and Reinberg, D. (1995). Common themes in assembly and function of eukaryotic transcription complexes. Annu. Rev. Biochem. 64, 533-561.