RNA helicase dynamics in pre-mRNA splicing

EMBO Journal

Volumn 19, Issue 23, 2000, Pages 6582-6591

  Schwer, B ^a   Meszaros, T ^a  

^a WEILL CORNELL MEDICAL COLLEGE   (United States)

Author keywords

DExH box helicases; Pre mRNA splicing; Prp22; Spliceosome disassembly

Indexed keywords

ADENOSINE TRIPHOSPHATE; MESSENGER RNA PRECURSOR; NUCLEOSIDE TRIPHOSPHATE; PROTEIN DEXH; PROTEIN PRP22; RNA; RNA HELICASE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; ENZYME ACTIVITY; ESTERIFICATION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FAMILY; RNA SPLICING; SPLICEOSOME;

EID: 0034423411     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/19.23.6582     Document Type: Article

References (41)

1. SLU7 and a novel activity, SSF1, act during the PRP16-dependent step of yeast pre-mRNA splicing
2. Prp43: An RNA helicase-like factor involved in spliceosome disassembly
3. Splicing of precursors to mRNA by the spliceosome
4. A putative ATP binding protein influences the fidelity of branchpoint recognition in yeast splicing
5. Genetic interactions of conserved regions in the DEAD-box protein Prp28p
6. The yeast PRP2 protein, a putative RNA-dependent ATPase, shares extensive sequence homology with two other pre-mRNA splicing factors
7. Requirement of the RNA helicase-like protein PRP22 for release of messenger RNA from spliceosomes
8. Unwinding RNA in Saccharomyces cerevisiae: DEAD-box proteins and related families
9. Dominant negative mutants of the yeast splicing factor Prp2 map to a putative cleft region in the helicase domain of DExD/H-box proteins
10. Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes
11. Mutational analysis of vaccinia virus nucleoside triphosphate phosphohydrolase II, a DExH box RNA helicase
12. The QRxGRxGRxxxG motif of the vaccinia virus DExH box RNA helicase NPH-II is required for ATP hydrolysis and RNA unwinding but not for RNA binding
13. The nucleoside triphosphatase and helicase activities of vaccinia virus NPH-II are essential for virus replication
14. Messenger RNA splicing in yeast: Clues to why the spliceosome is a ribonucleoprotein particle
15. A point mutation abolishes the helicase but not the nucleoside triphosphatase activity of hepatitis C virus NS3 protein
16. Mutational analysis of the yeast DEAH-box splicing factor Prp16
17. The DExH/D protein family database
18. Mutational analysis of hepatitis C virus RNA helicase
19. Hepatitis C virus NS3 RNA helicase domain with a bound oligonucleotide: The crystal structure provides insights into the mode of unwinding
20. The purified yeast pre-mRNA splicing factor PRP2 is an RNA-dependent NTPase
21. The DNA replication priming protein, PriA, is required for homologous recombination and double-strand break repair
22. A new RNA helicase isolated from HeLa cells that catalytically translocates in the 3' to 5' direction
23. Yeast mRNA splicing in vitro
24. Dynamic RNA-RNA interactions in the spliceosome
25. Mutational analysis of vaccinia virus nucleoside triphosphate phosphohydrolase I, a DNA-dependent ATPase of the DExH box family
26. Splicing of precursors to mRNA by the spliceosome
27. RNA-RNA interactions in the spliceosome: Unraveling the ties that bind
28. Mutational analysis of a DEAD box RNA helicase: The mammalian translation initiation factor eIF-4A
29. A dominant negative mutation in the conserved RNA helicase motif 'SAT' causes splicing factor PRF2 to stall in spliceosomes
30. Differential suppression of priA::kan phenotypes in Escherichia coli K-12 by mutations in priA, lexA and dnaC
31. Prp22, a DExH-box RNA helicase, plays two distinct roles in yeast pre-mRNA splicing
32. PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome
33. Mechanical devices of the spliceosome: Motors clocks, springs and things
34. Mutation of lysine-48 to arginine in the yeast RAD3 protein abolishes its ATPase and DNA helicase activities but not the ability to bind ATP
35. A novel role for a U5 snRNP protein in 3' splice site selection
36. Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism
37. The DEAH-box protein PRP22 is an ATPase that mediates ATP-dependent mRNA release from spliceosomes and unwinds RNA duplexes
38. The DEAH-box splicing factor Prp16 unwinds RNA duplexes in vitro
39. Protein functions in pre-mRNA splicing
40. Structure of the hepatitis C virus RNA helicase domain
41. ATPase-deficient mutants of the Escherichia coli DNA replication protein PriA are capable of catalyzing the assembly of active primosomes