Movement of nuclear poly(A) RNA throughout the interchromatin space in living cells

Current Biology

Volumn 9, Issue 6, 1999, Pages 285-291

  Politz, J C ^a   Tuft, R A ^a   Pederson, T ^a   Singer, R H ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL MIGRATION; CELL STRUCTURE; CHROMATIN STRUCTURE; DIFFUSION; FLUORESCEIN; MESSENGER RNA; NUCLEAR TRANSFER; PHOTOLYSIS; RNA SPLICING; TRANSCRIPTION REGULATION;

EUKARYOTA;

EID: 0033602460     PISSN: 09609822     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-9822(99)80136-5     Document Type: Article

References (29)

1. F Stutz M Rosbash Nuclear RNA export Genes Dev 12 1998 3303 3319
2. JE Dahlberg E Lund Functions of the GTPase Ran in RNA export from the nucleus Curr Opin Cell Biol 10 1998 400 408
3. IW Mattaj L Englmeier Nucleocytoplasmic transport: the soluble phase Annu Rev Biochem 67 1998 265 306
4. L Englmeier JC Olivo IW Mattaj Receptor-mediated substrate translocation through the nuclear pore complex without nucleotide triphosphate hydrolysis Curr Biol 9 1999 30 41
5. G Blobel Gene gating: a hypothesis Proc Natl Acad Sci USA 82 1985 8527 8529
6. Z Zachar J Kramer IP Mims PM Bingham Evidence for channeled diffusion of pre-mRNAs during nuclear RNA transport in metazoans J Cell Biol 121 1993 729 742
7. PS Agutter Models for solid-state transport: messenger RNA movement from nucleus to cytoplasm Cell Biol Int 18 1994 849 858
8. WF Marshall A Straight JF Marko J Swedlow A Dernburg A Belmont Interphase chromosomes undergo constrained diffusional motion in living cells Curr Biol 7 1997 930 939
9. J Wang LG Cao YL Wang T Pederson Localization of pre-messenger RNA at discrete nuclear sites Proc Natl Acad Sci USA 88 1991 7391 7395
10. MR Jacobson LG Cao YL Wang T Pederson Dynamic localization of RNase MRP RNA in the nucleolus observed by fluorescent RNA cytochemistry in living cells J Cell Biol 131 1995 1649 1658
11. MR Jacobson LG Cao KL Taneja RH Singer YL Wang T Pederson Nuclear domains of the RNA subunit of RNase P RNA J Cell Sci 110 1997 829 837
12. O Seksek J Biwersi AS Verkman Translational diffusion of macromolecule-sized solutes in cytoplasm and nucleus J Cell Biol 138 1997 131 142
13. JC Politz ES Browne DE Wolf T Pederson Intranuclear diffusion and hybridization state of oligonucleotides measured by fluorescence correlation spectroscopy in living cells Proc Natl Acad Sci USA 95 1998 6043 6048
14. JC Politz KL Taneja RH Singer Characterization of hybridization between syntheticoligodeoxynucleotides and RNA in living cells Nucleic Acids Res 23 1995 4946 4953
15. JC Politz RH Singer in situ reverse transcription for the detection of hybridization between oligonucleotides and their intracellular targets Methods 1999 in press
16. TJ Mitchison KE Sawin JA Theriot K Gee A Mallavarapu Caged fluorescent probes Methods Enzymol 291 1998 63 78
17. TJ Mitchison Polewards microtubule flux in the mitotic spindle: evidence from photoactivation of fluorescence J Cell Biol 109 1989 637 652
18. KL Taneja LM Lifshitz FS Fay RH Singer Poly(A) RNA codistribution with microfilaments: evaluation by in situ hybridization and quantitative digital imaging microscopy J Cell Biol 119 1992 1245 1260
19. A Einstein Investigations on the Theory of Brownian Movement 1956 Dover Publishing New York
20. MJ Saxton Single-particle tracking: effects of corrals Biophys J 69 1995 389 398
21. MJ Saxton Single-particle tracking: the distribution of diffusion coefficients Biophys J 72 1997 1744 1753
22. BP Olveczky AS Verkman Monte Carlo analysis of obstructed diffusion in three dimensions: application to molecular diffusion in organelles Biophys J 74 1998 2722 2730
23. EJ Steinmetz Pre-mRNA processing and the CTD of RNA polymerase II: the tail that wags the dog? Cell 89 1997 491 494
24. T Misteli DL Spector The cellular organization of gene expression Curr Opin Cell Biol 10 1998 323 331
25. T Pederson Thinking about a nuclear matrix J Mol Biol 277 1998 147 159
26. 2+ responses Science 280 1998 1763 1766
27. R Rizzuto W Carrington RA Tuft Digital imaging microscopy of living cells Trends Cell Biol 8 1998 288 292
28. WA Carrington RM Lynch ED Moore G Isenberg KE Forgarty FS Fay Superresolution three-dimensional images of fluorescence in cells with minimal light exposure Science 268 1995 1483 1487
29. RA Cardullo RM Mungovan DE Wolf Imaging membrane organization and dynamics TD Dewey Biophysical and Biochemical Aspects of Fluorescence Spectroscopy 1991 Plenum Publishing New York 231 260