A block to mRNA nuclear export in S. cerevisiae leads to hyperadenylation of transcripts that accumulate at the site of transcription

Molecular Cell

Volumn 7, Issue 4, 2001, Pages 887-898

  Jensen, Torben Heick ^a   Patricio, Kimberly ^a   McCarthy, Terri ^a   Rosbash, Michael ^a  

^a BRANDEIS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HEAT SHOCK PROTEIN 104; POLYADENYLIC ACID; PROTEIN DERIVATIVE; PROTEIN SSA4; UNCLASSIFIED DRUG;

ADENYLATION; ARTICLE; CELL STRAIN; PROTEIN STRUCTURE; PROTEIN TRANSPORT; RNA TRANSCRIPTION; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

EID: 0035022577     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(01)00232-5     Document Type: Article

References (40)

1. Arts, G.J., Fornerod, M., and Mattaj, I.W. (1998). Identification of a nuclear export receptor for tRNA. Curr. Biol. 8, 305-314.
2. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. (1994). Current Protocols in Molecular Biology (New York: Greene Publishing Associates).
3. Boorstein, W.R., and Craig, E.A. (1990). Structure and regulation of the SSA4 HSP70 gene of Saccharomyces cerevisiae. J. Biol. Chem. 265, 18912-18921.
4. Brown, C.E., and Sachs, A.B. (1998). Poly(A) tail length control in Saccharomyces cerevisiae occurs by message-specific deadenylation. Mol. Cell. Biol. 18, 6548-6559.
5. Custodio, N., Carmo-Fonseca, M., Geraghty, F., Pereira, H.S., Grosveld, F., and Antoniou, M. (1999). Inefficient processing impairs release of RNA from the site of transcription. EMBO J. 18, 2855-2866.
6. Eckner, R., Ellmeier, W., and Birnstiel, M.L. (1991). Mature mRNA 3′end formation stimulates RNA export from the nucleus. EMBO J. 10, 3513-3522.
7. Feldherr, C.M., Kallenbach, E., and Schultz, N. (1984). Movement of a karyophilic protein through the nuclear pores of oocytes. J. Cell Biol. 99, 2216-2222.
8. Forrester, W., Stutz, F., Rosbash, M., and Wickens, M. (1992). Defects in mRNA 3′-end formation, transcription initiation, and mRNA transport associated with the yeast mutation prp20: possible coupling of mRNA processing and chromatin structure. Genes Dev. 6, 1914-1926.
9. Gorlich, D., Dabrowski, M., Bischoff, F.R., Kutay, U., Bork, P., Hartmann, E., Prehn, S., and Izaurralde, E. (1997). A novel class of RanGTP binding proteins. J. Cell Biol. 138, 65-80.
10. Gorsch, L.C., Dockendorff, T.C., and Cole, C.N. (1995). A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/ NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes. J. Cell Biol. 129, 939-955.
11. Huang, Y., and Carmichael, G.G. (1996). Role of polyadenylation in nucleocytoplasmic transport of mRNA. Mol. Cell. Biol. 16, 1534-1542.
12. Hurt, E., Sträßer, K., Segref, A., Bailer, S., Schlaich, N., Presutti, C., Tollervey, D., and Jansen, R. (2000). Mex67p mediates nuclear export of a variety of RNA polymerase II transcripts. J. Biol. Chem. 275, 8361-8368.
13. Izaurralde, E., Kutay, U., von Kobbe, C., Mattaj, I.W., and Gorlich, D. (1997). The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus. EMBO J. 16, 6535-6547.
14. Katahira, J., Straßer, K., Podtelenikov, A., Mann, M., Jung, J.U., and Hurt, E. (1999). The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human. EMBO J. 18, 2593-2609.
15. Kutay, U., Lipowsky, G., Izaurralde, E., Bischoff, F.R., Schwarzmaier, P., Hartmann, E., and Gorlich, D. (1998). Identification of a tRNA-specific nuclear export receptor. Cell 1, 359-369.
16. Long, R.M., Elliott, D.J., Stutz, F., Rosbash, M., and Singer, R.H. (1995). Spatial consequences of defective processing of specific yeast mRNAs revealed by fluorescence in situ hybridization. RNA 1, 1071-1078.
17. Long, R.M., Singer, R.H., Meng, X., Gonzalez, I., Nasmyth, K., and Jansen, R. (1997). Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA. Science 277, 383-387.
18. Mattaj, I.W., and Englmeier, L. (1998). Nucleocytoplasmic transport: the soluble phase. Annu. Rev. Biochem. 67, 265-306.
19. Minvielle-Sebastia, L., Winsor, B., Bonneaud, N., and Lacroute, F. (1991). Mutations in the yeast RNA14 and RNA 15 genes result in an abnormal mRNA decay rate; sequence analysis reveals an RNA-binding domain in the RNA15 protein. Mol. Cell. Biol. 11, 3075-3087.
20. Murphy, R., and Wente, S.R. (1996). An RNA-export mediator with an essential nuclear export signal. Nature 383, 357-360.
21. Nakielny, S., and Dreyfuss, G. (1999). Transport of proteins and RNAs in and out of the nucleus. Cell 99, 677-690.
22. Roth, F.P., Hughes, J.D., Estep, P.W., and Church, G.M. (1998). Finding DNA regulatory motifs within unaligned noncoding sequences clustered by whole-genome mRNA quantitation. Nat Biotechnol 16, 939-945.
23. Rout, M.P., Aitchison, J.D., Suprapto, A., Hjertaas, K., Zhao, Y., and Chait, B.T. (2000). The yeast nuclear pore complex; composition, architecture, and transport mechanism. J. Cell Biol. 148, 635-651.
24. Saavedra, C.A., Hammell, C.M., Heath, C.V., and Cole, C.N. (1997). Yeast heat shock mRNAs are exported through a distinct pathway defined by Rip1p. Genes Dev. 11, 2845-2856.
25. Salles, F.J., Richards, W.G., and Strickland, S. (1999). Assaying the polyadenylation state of mRNAs. Methods 17, 38-45.
26. Santos-Rosa, H., Moreno, H., Simos, G., Segref, A., Fahrenkrog, B., Pante, N., and Hurt, E. (1998). Nuclear mRNA export requires complex formation between Mex67p and Mtr2p at the nuclear pores. Mol. Cell. Biol. 18, 6826-6838.
27. Sarkar, S., and Hopper, A.K. (1998). tRNA nuclear export in Saccharomyces cerevisiae: In situ hybridization analysis. Mol. Biol. Cell 9, 3041-3055.
28. Schmitt, C., von Kobbe, C., Bachi, A., Pante, N., Rodrigues, J.P., Boscheron, C., Rigaut, G., Wilm, M., Seraphin, B., Carmo-Fonseca, M., and Izaurralde, E. (1999). Dbp5, a DEAD-box protein required for mRNA export, is recruited to the cytoplasmic fibrils of the nuclear pore complex via a conserved interaction with CAN/Nup159p. EMBO J. 18, 4332-4347.
29. + RNA and nuclear pores. EMBO J. 16, 3256-3271.
30. Snay-Hodge, C.A., Colot, H.V., Goldstein, A.L., and Cole, C.N. (1998). Dbp5p/Rat8p is a yeast nuclear pore-associated DEAD-box protein essential for RNA export. EMBO J. 17, 2663-2676.
31. Stoffler, D., Fahrenkrog, B., and Aebi, U. (1999). The nuclear pore complex: from molecular architecture to functional dynamic. Curr. Opin. Cell Biol. 11, 391-401.
32. Strahm, Y., Fahrenkrog, B., Zenklusen, D., Rychner, E., Kantor, J., Rosbash, M., and Stutz, F. (1999). The RNA export factor Gle1p is located on the cytoplasmic fibrils of the NPC and physically interacts with the FG-nucleoporin Rip1p, the DEAD-box protein Rat8p/Dbp5p and a new protein Ymr255p. EMBO J. 18, 5761-5777.
33. Straßer, K., Bassler, J., and Hurt, E. (2000). Binding of the Mex67p/ Mtr2p heterodimer to FXFG, GLFG, and FG repeat nucleoporins is essential for nuclear mRNA export. J. Cell Biol. 150, 695-706.
34. Stutz, F., and Rosbash, M. (1998). Nuclear RNA export. Genes Dev. 72, 3303-3319.
35. Stutz, F., Kantor, J., Zhang, D., McCarthy, T., Neville, M., and Rosbash, M. (1997). The yeast nucleoporin Rip1p contributes to multiple export pathways with no essential role for its FG-repeat region. Genes Dev. 11, 2857-2868.
36. Tseng, S.S., Weaver, P.L., Liu, Y., Hitomi, M., Tartakoff, A.M., and Chang, T.-H. (1998). Dbp5p, a cytosolic RNA helicase is required for poly (A)+ RNA export. EMBO J. 17, 2651-2662.
37. Vainberg, I.E., Dower, K., and Rosbash, M. (2000). Nuclear export of heat shock and non-heat-shock mRNA occurs via similar pathways. Mol. Cell. Biol. 20, 3996-4005.
38. Watkins, J.L., Murphy, R., Emtage, J.L., and Wente, S. (1998). The human homologue of Saccharomyces cerevisiae Gle1p is required for poly(A)+ RNA export. Proc.Natl.Acad.Sci.USA 95, 6779-6784.
39. Wozniak, R.W., Rout, M.P., and Aitchison, J.D. (1998). Karyopherins and kissing cousins. Trends Cell Biol. 8, 184-188.
40. Zhao, J., Hyman, L.E., and Moore, C. (1999). Formation of mRNA 3′ ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis. Microbiol. Mol. Biol. Rev. 63, 405-445.