Genetic interference in Trypanosoma brucei by heritable and inducible double-stranded RNA

RNA

Volumn 6, Issue 7, 2000, Pages 1069-1076

  Shi, Huafang ^a   Djikeng, Appolinaire ^a   Mark, Tomer ^a   Wirtz, Elizabeth ^b   Tschudi, Christian ^a   Ullu, Elisabetta ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b ROCKEFELLER UNIVERSITY   (United States)

Author keywords

Double stranded RNA; Inducible expression; RNA interference; T7 RNA polymerase; Trypanosome

Indexed keywords

ACTIN; ALPHA TUBULIN; DOUBLE STRANDED RNA; MESSENGER RNA;

ARTICLE; CONTROLLED STUDY; DOWN REGULATION; GENE CONTROL; GENE DISRUPTION; GENE EXPRESSION; GENE INDUCTION; GENE TARGETING; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; RNA DEGRADATION; RNA STRUCTURE; TRYPANOSOMA BRUCEI;

ACTINS; ANIMALS; DNA-DIRECTED RNA POLYMERASES; DOSE-RESPONSE RELATIONSHIP, DRUG; DOWN-REGULATION; ELECTROPORATION; GENE EXPRESSION; GENETIC ENGINEERING; PHENOTYPE; PLASMIDS; PROMOTER REGIONS (GENETICS); PROTEIN SYNTHESIS INHIBITORS; PROTEINS; RNA, DOUBLE-STRANDED; TETRACYCLINE; TIME FACTORS; TRANSFECTION; TRYPANOSOMA BRUCEI BRUCEI; TUBULIN; VIRAL PROTEINS;

PROTOZOA; TRYPANOSOMA; TRYPANOSOMA BRUCEI;

EID: 0033928734     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838200000297     Document Type: Article

References (21)

1. Carruthers VB, Cross GA. 1992. High-efficiency clonal growth of bloodstream- and insect-form Trypanosoma brucei on agarose plates. Proc Natl Acad Sci USA 89:8818-8821.
2. Cogoni C, Irelan JT, Schumacher M, Schmidhauser TJ, Selker EU, Macino G. 1996. Transgene silencing of the al-1 gene in vegetative cells of Neurospora is mediated by a cytoplasmic effector and does not depend on DNA-DNA interactions or DNA methylation. EMBO J 15:3153-3163.
3. Cogoni C, Macino G. 1999a. Gene silencing in Neurospora crassa requires a protein homologous to RNA-dependent RNA polymerase. Nature 399:166-169.
4. Cogoni C, Macino G. 1999b. Posttranscriptional gene silencing in Neurospora by a RecQ DNA helicase. Science 286:2342-2344.
5. Deflorin J, Rudolf M, Seebeck T. 1994. The major components of the paraflagellar rod of Trypanosoma brucei are two similar, but distinct proteins which are encoded by two different gene loci. J Biol Chem 269:28745-28751.
6. Dobrowolski JM, Sibley LD. 1996. Toxoplasma invasion of mammalian cells is powered by the actin cytoskeleton of the parasite. Cell 84:933-939.
7. Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC. 1998. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391:806-811.
8. Ketting RF, Haverkamp TH, van Luenen HG, Plasterk RH. 1999. mut-7 of C. elegans, required for transposon silencing and RNA interference, is a homolog of Werner syndrome helicase and RNaseD. Cell 99:133-141.
9. Lohmann JU, Endl I, Bosch TC. 1999. Silencing of developmental genes in Hydra. Dev Biol 214:211-214.
10. Misquitta L, Paterson BM. 1999. Targeted disruption of gene function in Drosophila by RNA interference (RNA-i): A role for nautilus in embryonic somatic muscle formation. Proc Natl Acad Sci USA 96:1451-1456.
11. Mortara RA. 1989. Studies on trypanosomatid actin. I. Immunochemical and biochemical identification. J Protozool 36:8-13.
12. Ngo H, Tschudi C, Gull K, Ullu E. 1998. Double-stranded RNA induces mRNA degradation in Trypanosoma brucei. Proc Natl Acad Sci USA 95:14687-14692.
13. Sanchez Alvarado A, Newmark PA. 1999. Double-stranded RNA specifically disrupts gene expression during planarian regeneration. Proc Natl Acad Sci USA 96:5049-5054.
14. Tabara H, Grishok A, Mello CC. 1998. RNAi in C. elegans: Soaking in the genome sequence. Science 282:430-431.
15. Tabara H, Sarkissian M, Kelly WG, Fleenor J, Grishok A, Timmons L, Fire A, Mello CC. 1999. The rde-1 gene, RNA interference, and transposon silencing in C. elegans. Cell 99:123-132.
16. Timmons L, Fire A. 1998. Specific interference by ingested dsRNA. Nature 395:854.
17. Wassenegger M, Pelissier T. 1998. A model for RNA-mediated gene silencing in higher plants. Plant Mol Biol 37:349-362.
18. Wirtz E, Clayton C. 1995. Inducible gene expression in trypanosomes mediated by a prokaryotic repressor. Science 268:1179-1183.
19. Wirtz E, Hartmann C, Clayton C. 1994. Gene expression mediated by bacteriophage T3 and T7 RNA polymerases in transgenic trypanosomes. Nucleic Acids Res 22:3887-3894.
20. Wirtz E, Hoek M, Cross GAM. 1998. Regulated processive transcription of chromatin by T7 RNA polymerase in Trypanosoma brucei. Nucleic Acids Res 26:4626-4634.
21. Wirtz E, Leal S, Ochatt C, Cross GA. 1999. A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei. Mol Biochem Parasitol 99:89-101.