Enzymic properties of recombinant BACE2

European Journal of Biochemistry

Volumn 269, Issue 22, 2002, Pages 5668-5677

  Kim, Yong Tae ^a   Downs, Deborah ^a,b   Wu, Shili ^a,b   Dashti, Azar ^a,b   Pan, Yujun ^a   Zhai, Peng ^a,b   Wang, Xinjuan ^a,b,c   Zhang, Xuejun C ^a   Lin, Xinli ^a,b,d  

^a OKLAHOMA MEDICAL RESEARCH FOUNDATION   (United States)

^b ProteomTech Inc   (United States)

^c PEKING UNIVERSITY HEALTH SCIENCE CENTER   (China)

^d University of Oklahoma Medical Center   (United States)

Author keywords

secretase; Alzheimer's disease; B amyloid precursor protein; BACE2; Propeptide processing enzyme

Indexed keywords

ASPARTIC PROTEINASE; BETA SECRETASE; BETA SECRETASE2; PEPSIN A; PROLINE; RECOMBINANT ENZYME; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL INCLUSION; ENZYME SPECIFICITY; ESCHERICHIA COLI; MOLECULAR BIOLOGY; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; AMYLOID PRECURSOR PROTEIN SECRETASES; ASPARTIC ENDOPEPTIDASES; BINDING SITES; CATALYTIC DOMAIN; CIRCULAR DICHROISM; CLONING, MOLECULAR; ENDOPEPTIDASES; ESCHERICHIA COLI; HUMANS; HYDROGEN-ION CONCENTRATION; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SERINE; TEMPERATURE;

ESCHERICHIA COLI;

EID: 0036438915     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2002.03277.x     Document Type: Article

References (48)

1. Selkoe, D.J. (1997) Alzheimer's disease: Genotypes, phenotypes, and treatments. Science 275, 630-631.
2. Hardy, J. (1997) The Alzheimer family of diseases: Many ethiologies, one pathogenesis? Proc. Natl. Acad. Sci. USA 94, 2095-2097.
3. Wolfe, M.S., Xia, W., Ostaszewski, B.L., Diehl, T.S., Kimberly, W.T. & Selkoe, D.J. (1999) Two transmembrane aspartates in presenilin-1 required for presenilin endoproteolysis and γ-secretase activity. Nature 398, 513-517.
4. Kimberly, W.T., Xia, W., Rahmati, T., Wolfe, M.S. & Selkoe, D.J. (2000) The transmembrane aspartates in presenilin 1 and 2 are obligatory for γ-secretase activity & amyloid β-protein generation. J. Biol. Chem. 275, 3173-3178.
5. Sinha, S. & Lieberburg, I. (1999) Cellular mechanisms of β-amyloid production and secretion. Proc. Natl. Acad. Sci. USA 96, 11049-11053.
6. Sinha, S., Anderson, J.P., Barbour, R., Basi, G.S., Caccavello, R., Davis, D., Doan, M., Dovey, H.F., Frigon, N., Hong, J., Jacobson-Croak, K., Jewett, N., Keim, P., Knops, J., Lieberburg, I., Power, M., Tan, H., Tatsuno, G., Tung, J., Schenk, D., Seubert, P., Suomensaari, S.M., Wang, S., Walker, D., John, V., et al. (1999) Purification and cloning of amyloid precursor protein β-secretase from human brain. Nature 402, 537-540.
7. Vassar, R., Bennett, B.D., Babu-Khan, S., Kahn, S., Mendiaz, E.A., Denis, P., Teplow, D.B., Ross, S., Amarante, P., Loeloff, R., Luo, Y., Fisher, S., Fuller, J., Edenson, S., Lile, J., Jarosinski, M.A., Biere, A.L., Curran, E., Burgess, T., Louis, J.C., Collins, F., Treanor, J., Rogers, G. & Citron, M. (1999) β-Secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 286, 735-741.
8. Yan, R., Bienkowski, M.J., Shuck, M.E., Miao, H., Tory, M.C., Pauley, A.M., Brashier, J.R., Stratman, N.C., Mathews, W.R., Buhl, A.E., Carter, D.B., Tomasselli, A.G., Parodi, L.A., Heinrikson, R.L. & Gurney, M.E. (1999) Membrane-anchored aspartyl protease with Alzheimer's disease β-secretase activity. Nature 402, 533-537.
9. Hussain, I., Powell, D., Howlett, D.R., Tew, D.G., Meek, T.D., Chapman, C., Gloger, I.S., Murphy, K.E., Southan, C.D., Ryan, D.M., Smith, T.S., Simmons, D.L., Walsh, F.S., Dingwall, C. & Christie, G. (1999) Identification of a novel aspartic protease (Asp2) as β-secretase. Mol. Cell. Neurosci. 14, 419-427.
10. Lin, X., Koelsch, G., Wu, S., Downs, D., Dashti, A. & Tang, J. (2000) Human aspartic protease memapsin 2 cleaves the β-secretase site of β-amyloid precursor protein. Proc. Natl. Acad. Sci. USA 97, 1456-1460.
11. Hong, L., Koelsch, G., Lin, X., Wu, S., Terzyan, S., Ghosh, A.K., Zhang, X.C. & Tang, J. (2000) Structure of the protease domain of memapsin 2 (β-secretase) complexed with inhibitior. Science 290, 150-153.
12. Luo, Y., Bolon, B., Kahn, S., Bennett, B.D., Babu-Khan, S., Denis, P., Fan, W., Kha, H., Zhang, J., Gong, Y., Martin, L., Louis, J.C., Yan, Q., Richards, W.G., Citron, M. & Vassar, R. (2001) Mice deficient in BACE1, the Alzheimer's beta-secretase, have normal phenotype and abolished beta-amyloid generation. Nature Neurosci. 4, 231-232.
13. Cai, H., Wang, Y., McCarthy, D., Wen, H., Borchelt, D., Price, D.L. & Wong, P.C. (2001) BACE1 is the major β-secretase for generation of Aβ peptides by neurons. Nature Neurosci. 4, 233-234.
14. Saunders, A.J., Kim, T.-W., Tanzi, R.E., Fan, W., Bennett, B.D., Babu-Kahn, S., Luo, Y., Louis, J.-C., McCaleb, M., Citron, M., Vassar, R. & Richards, W.G. (1999) BACE maps to chromosome 11 and a BACE homolog, BACE2, reside in the obligate down syndrome region of chromosome 21. Science 286, 1255a-1255a.
15. Acquati, F., Accarino, M., Nucci, C., Fumagalli, P., Jovine, L., Ottolenghi, S. & Taramelli, R. (2000) The gene encoding DRAP (BACE2), a glycosylated transmembrane protein of the aspartic protease family, maps to the Down critical region. FEBS Lett. 468, 59-64.
16. Solans, A., Estivill, X. & de La Luna, S. (2000) A new aspartyl protease on 21q22.3, BACE2, is highly similar to Alzheimer's amyloid precursor protein β-secretase. Cytogenet. Cell. Genet. 89, 177-184.
17. Tang, J. & Wong, R.N. (1987) Evolution in the structure and function of aspartic proteases. J. Cell. Biochem. 33, 53-63.
18. Jutras, I. & Reudelhuber, T.L. (1999) Prorenin processing by cathepsin B in vitro and in transfected cells. FEBS Lett. 443, 48-52
19. Creemers, J.W., Dominguez, D.I., Plets, E., Serneels, L., Taylor, N.A., Multhaup, G., Craessaerts, K., Annaert, W. & De Strooper, B. (2001) Processing of β-secretase by furin and other members of the proprotein convertase family. J. Biol. Chem. 276, 4211-4217.
20. Bennett, B.D., Denis, P., Haniu, M., Teplow, D.B., Kahn, S., Louis, J.C., Citron, M. & Vassar, R. (2000) A furin-like convertase mediates propeptide cleavage of BACE, the Alzheimer's β-secretase. J. Biol. Chem. 275, 37712-37717.
21. Shi, X.P., Chen, E., Yin, K.C., Na, S., Garsky, V.M., Lai, M.T., Li, Y.M., Platchek, M., Register, R.B., Sardana, M.K., Tang, M.J., Thiebeau, J., Wood, T., Shafer, J.A. & Gardell, S.J. (2001) The pro domain of β-secretase does not confer strict zymogen-like properties but does assist proper folding of the protease domain. J. Biol. Chem. 276, 10366-10373.
22. Tatnell, P.J., Powell, D.J., Hill, J., Smith, T.S., Tew, D.G. & Kay, J. (1998) Napsins: new human aspartic proteinases. Distinction between two closely related genes. FEBS Lett. 441, 43-48.
23. Chuman, Y., Bergman, A., Ueno, T., Saito, S., Sakaguchi, K., Alaiya, A.A., Franzen, B., Bergman, T., Arnott, D., Auer, G., Appella, E., Jornvall, H. & Linder, S. (1999) Napsin A, a member of the aspartic protease family, is abundantly expressed in normal lung and kidney tissue and is expressed in lung adenocarcinomas. FEBS Lett. 462, 129-134.
24. Hussain, I., Powell, D.J., Howlett, D.R., Chapman, G.A., Gilmour, L., Murdock, P.R., Tew, D.G., Meek, T.D., Chapman, C., Schneider, K., Ratcliffe, S.J., Tattersall, D., Testa, T.T., Southan, C., Ryan, D.M., Simmons, D.L., Walsh, F.S., Dingwall, C. & Christie, G. (2000) ASP1 (BACE2) cleaves the amyloid precursor protein at the β-secretase site. Mol. Cell. Neurosci. 16, 609-619.
25. Farzan, M., Schnitzler, C.E., Vasilieva, N., Leung, D. & Choe, H. (2000) BACE2, a β-secretase homolog, cleaves at the β site and within the amyloid-β region of the amyloid-β precursor protein. Proc. Natl. Acad. Sci. USA 97, 9712-9717.
26. Marcinkiewicz, M. & Seidah, N.G. (2000) Coordinated expression of β-amyloid precursor protein and the putative β-secretase BACE and α-secretase ADAM10 in mouse and human brain. J. Neurochem. 75, 2133-2143.
27. Bennett, B.D., Babu-Khan, S., Loeloff, R., Louis, J.C., Curran, E., Citron, M. & Vassar, R. (2000) Expression analysis of BACE2 in brain and peripheral tissues. J. Biol. Chem. 275, 20647-20651.
28. Lin, X., Lin, Y. & Tang, J. (1994) Relationships of human immunodeficiency virus protease with eukaryotic aspartic proteases. Methods Enzymol. 241, 195-224.
29. Schroeter, E.H., Kisslinger, J.A. & Kopan, R. (1998) Notch-1 signalling requires ligand-induced proteolytic release of intracellular domain. Nature 393, 382-386.
30. Leatherbarrow, R.J. (1998) Grafit, Version 4.0 Staines, UK.
31. Kim, Y.T., Muramatsu, T. & Takahashi, K. (1995) Leader peptidase from E. coli: Overexpression, characterization, and inactivation by modification of tryptophan residues 300 and 310 with N-bromosuccinimide. J. Biochem. (Tokyo) 117, 535-544.
32. Jeohn, G.H. & Takahashi, K. (1995) Purification and characterization of a vasoactive intestinal polypeptide-degrading endoprotease from porcine antral mucosal membranes. J. Biol. Chem. 270, 7809-7815.
33. Lee, M.J., Kang, B.S., Kim, D.S., Kim, Y.T., Kim, S.K., Chung, K.H., Kim, J.K., Nam, K.S., Lee, Y.C. & Kim, C.H. (1997) Processing of an intracellular immature pullulanase to the mature form involves enzymatic activation and stabilization in alkaliphilic Bacillus sp. S-1. J. Biochem. Mol. Biol. 30, 46-54.
34. Jeohn, G.H., Serizawa, S., Iwamatsu, A. & Takahashi, K. (1995) Isolation and characterization of gastric trypsin from the microsomal fraction of porcine gastric antral mucosa. J. Biol. Chem. 270, 14748-14755.
35. Ermoliff, J., Loy, J.A., Koelsch, G. & Tang, J. (2000) Proteolytic activation of recombinant pro-memapsin 2 (pro-β-secretase) studied with new fluorogenic substrates. Biochemistry 39, 12450-12456.
36. Khan, A.R., Khazanovich-Bernstein, N., Bergmann, E.M. & James, M.N. (1999) Structural aspects of activation pathways of aspartic protease zymogens and viral 3C protease precursors. Proc. Natl. Acad. Sci. USA 96, 10968-10975.
37. Hussain, I., Christie, G., Schneider, K., Moore, S. & Dingwall, C. (2001) Prodomain processing of Asp1 (BACE2) is autocatalytic. J. Biol. Chem. 276, 23322-23328.
38. Yan, R., Munzner, J.B., Shuck, M.E. & Bienkowski, M.J. (2001) BACE2 functions as an alternative α-secretase in cells. J. Biol. Chem. 276, 34019-34027.
39. Mallender, W.D., Yager, D., Onstead, L., Nichols, M.R., Eckman, C., Sambamurti, K., Kopcho, L.M., Marcinkeviciene, J., Copeland, R.A. & Rosenberry, T.L. (2001) Characterization of recombinant, soluble β-secretase from an insect cell expression system. Mol. Pharmacol. 59, 619-626.
40. Charlwood, J., Dingwall, C., Matico, R., Hussain, I., Johanson, K., Moore, S., Powell, D.J., Skehel, J.M., Ratcliffe, S., Clarke, B., Trill, J., Sweitzer, S. & Camilleri, P. (2001) Characterization of the glycosylation profiles of Alzheimer's β-secretase protein Asp-2 expressed in a variety of cell lines. J. Biol. Chem. 276, 16739-16748.
41. Tang, J., Wolf, S., Caputto, R. & Trucco, R.E. (1959) Isolation and crystallization of gastricsin from human gastric juice. J. Biol. Chem. 234, 1174-1178.
42. Barrett, A.J. (1970) Cathepsin D. Purification of isoenzymes from human and chicken liver. Biochem. J. 117, 601-607.
43. Cawley, N.X., Wong, M., Pu, L.P., Tam, W. & Loh, Y.P. (1995) Secretion of yeast aspartic protease 3 is regulated by its carboxyterminal tail: Characterization of secreted YAP3p. Biochemistry 34, 7430-7437.
44. Pickens, P.T., Bumpus, F.M., Lloyd, A.M., Smeby, R.R. & Page, I.H. (1965) Measurement of renin activity in human plasma. Circ. Res. 17, 438-448.
45. Figueiredo, A.F.S., Takii, Y., Tsuji, H., Kato, K. & Inagami, T. (1983) Rat kidney renin and cathepsin D: Purification and comparison of properties. Biochemistry 22, 5476-5481.
46. Dev, I.K. & Ray, P.H. (1984) Rapid assay and purification of a unique signal peptidase that processes the prolipoprotein from Escherichia coli B. J. Biol. Chem. 259, 11114-11120.
47. Haniu, M., Denis, P., Young, Y., Mendiaz, E.A., Fuller, J., Hui, J.O., Bennett, B.D., Kahn, S., Ross, S., Burgess, T., Katta, V., Rogers, G., Vassar, R. & Citron, M. (2000) Characterization of Alzheimer's β-secretase protein BACE. J. Biol. Chem. 275, 21099-21106.
48. Kraulis, P.J. (1991) Molscript: A program to produce both detailed and schematic plots of protein structures. J. Appl. Crystallogr. 24, 946-950.