The Pro Domain of β-Secretase Does Not Confer Strict Zymogen-like Properties but Does Assist Proper Folding of the Protease Domain

Journal of Biological Chemistry

Volumn 276, Issue 13, 2001, Pages 10366-10373

  Shi, Xiao Ping ^a   Chen, Elizabeth ^a   Yin, Kuo Chang ^a   Na, Sang ^a   Garsky, Victor M ^a   Lai, Ming Tain ^a   Li, Yue Ming ^a   Platchek, Michael ^a   Register, R Bruce ^a   Sardana, Mohinder K ^a   Tang, Mei Jy ^a   Thiebeau, James ^a   Wood, Theresa ^a   Shafer, Jules A ^a   Gardell, Stephen J ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MEMBRANES; CELLS; POLYPEPTIDES;

CYTOSOLIC DOMAINS;

ENZYMES;

AMYLOID BETA PROTEIN; AMYLOID PRECURSOR PROTEIN; ASPARTIC PROTEINASE; BETA SECRETASE; CONCANAVALIN A; ENZYME PRECURSOR; GUANIDINE; PROTEINASE; BACE1 PROTEIN, HUMAN; BACE2 PROTEIN, HUMAN; BETA SITE APP CLEAVING ENZYME 2; BETA-SITE APP CLEAVING ENZYME 2; COMPLEMENTARY DNA; FURIN; PROTEIN PRECURSOR; RECOMBINANT PROTEIN; SECRETASE; SUBTILISIN;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME RENATURATION; INSECT CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FOLDING; AMINO ACID SEQUENCE; ANIMAL; BACULOVIRUS; BINDING SITE; CATALYSIS; CELL LINE; CHEMISTRY; CULTURE MEDIUM; DOSE RESPONSE; DRUG ANTAGONISM; HUMAN; IMMUNOBLOTTING; INSECT; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR GENETICS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN TERTIARY STRUCTURE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SERODIAGNOSIS; TIME;

ANIMALIA; INSECTA; UNIDENTIFIED BACULOVIRUS;

AMINO ACID SEQUENCE; AMYLOID PRECURSOR PROTEIN SECRETASES; ANIMALS; ASPARTIC ENDOPEPTIDASES; BACULOVIRIDAE; BINDING SITES; CATALYSIS; CELL LINE; CONCANAVALIN A; CULTURE MEDIA, CONDITIONED; DNA, COMPLEMENTARY; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASES; FURIN; GUANIDINE; HUMANS; IMMUNOBLOTTING; INSECTS; KINETICS; MOLECULAR SEQUENCE DATA; PRECIPITIN TESTS; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN PRECURSORS; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SUBTILISINS; TIME FACTORS;

EID: 0035971166     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M009200200     Document Type: Article

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