Understanding the art of producing protein and nonprotein molecules in Escherichia coli

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 19, Issue 3, 2001, Pages 251-267

  Balbas P ^a  

^a UNIVERSIDAD AUTÓNOMA DEL ESTADO DE MORELOS   (Mexico)

Author keywords

Bioreactor; Biotechnology; Expression; Promoters; Transgenic

Indexed keywords

CHAPERONE; HYBRID PROTEIN; PROTEIN SUBUNIT;

BIOREACTOR; BIOTECHNOLOGY; ESCHERICHIA COLI; NONHUMAN; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN MODIFICATION; PROTEIN PROCESSING; PROTEIN SECRETION; PROTEIN SYNTHESIS; PROTEIN TARGETING; REVIEW; RNA STABILITY; RNA SYNTHESIS; TRANSGENE;

ESCHERICHIA COLI; RECOMBINANT PROTEINS; RNA, MESSENGER;

EID: 0035169206     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/MB:19:3:251     Document Type: Review

References (128)

1. Designing expression plasmid vectors in E coli
2. Design and construction of expression plasmid vectors in Escherichia coli
3. Plasmid vector pBR322 and its special-purpose derivatives, a review
4. Recombinant protein expression in Escherichia coli
5. Overproduction of proteins in E. coli, vectors, hosts and strategies
6. Gene expression technology
7. Strategies for optimizing heterologous protein expression in Escherichia coli
8. Gene fusion expression systems in E. coli
9. Strategies for achieving high-level expression of genes in Escherichia coli
10. Recent developments in heterologous protein production in Escherichia coli
11. Expression and secretion of proteins in E. coli
12. Strategies in high level expression of recombinant proteins in E. coli
13. Alternative regulation principles for the production of recombinant proteins in Escherichia coli
14. Gene expression in recombinant Escherichia coli
15. Optimization of heterologous protein production in Escherichia coli
16. RNA polymerse-promoter interactions, the comings and goings of RNA polymerase
17. Identification of an UP element consensus sequence for bacterial promoters
18. Activation and repression of E. coli promoters
19. Prokaryotic promoters in biotechnology
20. Construction and characterization of a biotin-regulated gene expression system in Escherichia coli
21. Participation of IHF and a distant UP element in the stimulation of the phage lambda PL promoter
22. Development and characterization of an oxygen-dependent inducible promoter system, the modified nar promoter in a mutant Escherichia coli
23. A family of expression vectors based on the rrnB P2 promoter of Escherichia coli
24. High level heterologous expression in E. coli using anaerobically-activated nirB promoter
25. Formation of recombinant proteins in Escherichia coli under control of a nitrogen regulated promoter at low and high cell densities
26. The use of elements of the E. coli NTR-system for the design of an optimized recombinant expression system for high cell density cultivations
27. Use of a modified tryptophanase promoter to direct high-level expression of foreign proteins in E. coli
28. Parameters affecting gene expression from the Pm promoter in Gram-negative bacteria
29. Pm promoter expression mutants and their use in broad-host-range RK2 plasmid vectors
30. A modular set of prokaryotic and eukaryotic expression vectors
31. Control of cloned gene expression by promoter inversion in vivo, construction of the heat-pulse-activated att-nutL-p-att-N module
32. Control of cloned gene expression by promoter inversion in vivo, construction of improved vectors with a multiple cloning site and the Ptac promoter
33. A versatile and highly repressible Escherichia coli expression espression system based on invertible promoters, expression of a gene encoding a toxic product
34. Transcription attenuation, in Escherichia coli and Salmonella
35. Ribosomal RNA operon antitermination. Function of leader spacer region boxB-boxA sequences and their conservation in these microorganisms
36. Antitermination of E. coli rRNA transcription is caused by a control region segment containing lambda nut-like sequences
37. The decay of messenger RNA
38. The ompA 5′untranslated RNA segment functions in Escherichia coli as a growth-rate regulated mRNA stabilizer whose activity is unrelated to translational efficiency
39. A 5′terminal stem-loop structure can stabilize mRNA in Escherichia coli
40. Co-existing structures of a mRNA stability determinant
41. Increased protein expression through improved ribosome-binding sites obtained by library mutagenesis
42. Translational initiation frequency of atp genes from E. coli, identification of an intercistronic sequence that enhances translation
43. A novel sequence element derived from bacteriophage T7 mRNA acts as an enhancer of translation of the lacZ gene in E. coli
44. The downstream box, an efficient and independent translation initiation signal in E. coli
45. A role for mRNA secondary structure in the control of translation initiation
46. Specialized ribosome system, preferential translation of a single mRNA species by a subpopulation of mutated ribosomes
47. Construction and characterization of a specialized ribosome system for the overproduction of proteins in Escherichia coli
48. Engineering of bacterial ribosomes, replacement of all seven Escherichia coli rRNA operons by a single plasmid-encoded operon
49. Overexpression of an mRNA dependent on rare codons inhibits protein synthesis and cell growth
50. Proteases and their targets in Escherichia coli
51. Secretion-dependent proteolysis of heterologous proteins by recombinant Escherichia coli is connected to an increased activity of the energy-generating dissimilatory pathway
52. Upstream strategies to minimize proteolytic degradation upon recombinant production in Escherichia coli
53. Dynamics of in vivo protein aggregation, building inclusion bodies in recombinant bacteria
54. Protein folding in vivo and renaturation of recombinant proteins from inclusion bodies
55. Plasmid encoded protein, the principal factor in the "metabolic burden" associated with recombinant bacteria
56. Metabolic load and heterologous gene expression
57. Metabolic approaches for the optimisation of recombinant fermentation processes
58. Flow cytometric analisis of metabolic stress due to recombinant plasmids and proteins in Escherichia coli production strains
59. Molecular cloning by plasmid vectors
60. Chromosomal editing in Escherichia coli, vectors for DNA integration and excision
61. High-level expression of eukaryotic polypeptides from bacterial chromosomes
62. A chromosomal expression vector for Escherichia coli based on the bacteriophage Mu
63. Review: Optimizing inducer and culture conditions for expression of foreign proteins under the control of the lac promoter
64. Growth rate-optimised tRNA abundance and codon usage
65. Exponentially fed-batch cultures as an alternative to chemostats, the case of penicillin acylase production by recombinant E. coli
66. Products and bioprocesses based on genetically modified organisms, Review of bioengineering issues and trends in the literature. Asia Pac.
67. High cell-density culture of Escherichia coli
68. Culture conditions differentially affect the translation of individual mRNAs
69. Recombinant protein expression in high cell density fed-batch cultures of Escherichia coli
70. Control of in vivo proteolysis in the production of recombinant proteins
71. Polynucleotide phosphorylase and ribonuclease II are required for cell viability and mRNA turnover in Escherichia coli K-12
72. Effects of glycine supplement on protein production and release in recombinant Escherichia coli
73. Refolding of recombinant proteins
74. Protein folding by Escherichia coli chaperones and chaperonins
75. Expression of correctly folded proteins in Escherichia coli
76. Molecular chaperones, folding catalysts, and the recovery of active recombinant proteins from E. coli. To fold and refold
77. ClpB and HtpG facilitate de novo protein folding in stressed Escherichia coli cells
78. Protein folding
79. Efficient folding with multiple disulfide bonds in the Escherichia coli cytoplasm
80. Chaperone-mediated protein folding
81. How chaperones fold proteins
82. How chaperones protect virgin proteins
83. Oxidative refloding chromatography, folding of the scorpion toxin Cn5
84. Lysozyme refolding with immobilized GroEL column chromatography
85. The production and characterization of an immobilised chaperonin system
86. Effect of additives on refolding of a denatured protein
87. Proline inhibits aggregation during protein refolding
88. Escherichia coli translocase, the unravelling of a molecular machine
89. Protein targeting to the bacterial cytoplasmic membrane
90. Secretion of recombinant proteins by Gram-negative bacteria
91. Leaderless polypeptides efficiently extracted from whole cells by osmotic shock
92. Translational level is a critical factor for the secretion of heterologous proteins in Escherichia coli
93. An Escherichia coli host strain useful for efficient production of secreted recombinant protein
94. Expression of eukaryotic proteins in soluble form in Escherichia coli
95. Targeting and assembly of periplasmic and outer-membrane proteins in E. coli
96. Strategies for gene fusions
97. Bypassing the periplasm, reconstitution of the AcrAB multidrug efflux pump of E. coli
98. Affinity fusion strategies for detection, purification and immobilization of recombinant proteins
99. Prokaryotic gene fusion expression systems and their use in structural and functional studies of proteins
100. Fusion proteins in biotechnology
101. Modification with a phosphorilation tag of PKA in the TraT-base display vector of Escherichia coli
102. Insertional gene fusion technology
103. Vector engineering anomalies, impact on fusion protein purification performance
104. An Escherichia coli expression vector that allows recovery of proteins with native N-termini from purified calmodulin-binding peptide fusions
105. The use of recombinant fusion proteases in the affinity purification of recombinant proteins
106. Structure and function of the methionine aminopeptidases
107. Bacterial aminopeptidases, properties and functions
108. New tools for metabolic engineering of Escherichia coli
109. Engineering the central metabolism of Escherichia coli
110. Engineering a living cell to desired metabolite concentrations and fluxes, pathways with multifunctional enzymes
111. Alteration of growth yield by overexpression of ppc and pck in Escherichia coli
112. A direct comparison of approaches for increasing carbon flow to aromatic biosynthesis in Escherichia coli
113. The challenges and promise of metabolic engineering
114. Cloning of two pyruvate kinase isoenzyme structural genes from Escherichia coli, the relative roles of these enzymes in pyruvate biosinthesis
115. Pathway engineering for the production of aromatic compounds in E. coli
116. Metabolic engineering of Escherichia coli for the production of aromatic compounds
117. Metabolic network analisis. A powerful tool in metabolic engineering
118. Use and limitatons of modular control analysis in medicine and biotechnology
119. A general defficition of metabolic pathways useful for systematic organization and analysis of complex metabolic networks
120. Metabolic pathway analisis, basic concepts and scientific applications in the post-genomic era
121. Theory for the systemic definition of metabolic pathways and their use in interpreting metabolic function from a pathway-oriented perspective
122. Evolution of metabolism, a new method for the comparison of metabolic pathways using genomics information