Expression of eukaryotic proteins in soluble form in Escherichia coli

Protein Expression and Purification

Volumn 12, Issue 2, 1998, Pages 159-165

  Zhang, Yang ^a   Olsen, David R ^a   Nguyen, Kimyen B ^a   Olson, Pamela S ^a   Rhodes, Eric T ^b   Mascarenhas, Desmond ^a  

^a Celtrix Pharmaceuticals   (United States)

^b Palo Alto CA 94304 USA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GROWTH FACTOR; HYBRID PROTEIN; PROTEIN EXPRESSION; SOLUBILITY;

ESCHERICHIA COLI; EUKARYOTA;

EID: 0032030415     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1997.0834     Document Type: Article

References (38)

1. Wilkinson D. L., Harrison R. G. Predicting the solubility of recombinant proteins inEscherichia coli. Bio/Technology. 9:1991;443-448.
2. Schein C. H. Production of soluble recombinant proteins in bacteria. Bio/Technology. 7:1989;1141-1149.
3. LaVallie E. R., McCoy J. M. Gene fusion expression systems inEscherichia coli. Curr. Opin. Biotechnol. 6:1995;501-506.
4. di Guan C., Li P., Riggs P. D., Inouye H. Vectors that facilitate the expression and purification of foreign peptides inEscherichia coli. Gene. 67:1988;21-30.
5. Smith D. B., Johnson K. S. Single-step purification of polypeptides expressed inEscherichia coli. Gene. 67:1988;31-40.
6. Butt T. R., Jonnalagadda S., Monia B. P., Sternberg E. J., Marsh J. A., Stadel J. M., Ecker D. J., Crooke S. T. Ubiquitin fusion augments the yield of cloned gene products inEscherichia coli. Proc. Natl. Acad. Sci. USA. 86:1989;2540-2544.
7. LaVallie E. R., DiBlasio E. A., Kovacic S., Grant K. L., Schendel P. F., McCoy J. M. A thioredoxin gene fusion expression system that circumvents inclusion body formation in theE. coli. Biotechnology. 11:1993;189-193.
8. Bardwell J. C. A., McGovern K., Beckwith J. Identification of a protein required for disulfide bond formation in vivo. Cell. 67:1991;581-589.
9. Martin J. L., Bardwell J. C. A., Kuriyan J. Crystal structure of the DsbA protein required for disulphide bond formation in vivo. Nature. 365:1993;464-468.
10. Thorstenson Y. T., Zhang Y., Olson P. S., Mascarenhas D. Leaderless polypeptides efficiently extracted from whole cells by osmotic shock. J. Bacteriol. 179:1997;5333-5339.
11. Madisen L., Webb N. R., Rose T. M., Marquardt H., Ikeda T., Twardzik D. Transforming growth factor-β2: cDNA cloning and sequence analysis. DNA. 7:1988;1-8.
12. Rinderknecht E., Humbel R. E. The amino acid sequence of human insulin-like growth factor I and its structural homology with proinsulin. J. Biol. Chem. 253:1978;2769-2776.
13. Sommer A., Maack C. A., Spratt S. K., Mascarenhas D., Tressel T. J., Rhodes E. T. Molecular genetics and actions of recombinant insulin-like growth factor binding protein-3. Modern Concepts of Insulin-Like Growth Factors. 1991;Elsevier, Amsterdam. p. 715-728.
14. Keat-Chye Cheah S. S., Porter A. G. Expression and processing of human rhinovirus type 14 polypeptide precursors inEscherichia coli. Gene. 69:1988;265-274.
15. Shimizu N., Fukuzono S., Fujimori K., Park K., Takeshita T., Ishibashi T. Biological activity of brain-derived neurotrophic factor with mismatched disulfide linkages produced byEscherichia coli. Biosci. Biotech. Biochem. 60:1996;971-974.
16. Rentsch J., Levens N., Chiesi M. Recombinant ob-gene product reduces food intake in fasted mice. Biochem. Biophys. Res. Commun. 214:1995;131-136.
17. Lin L. F., Doherty D. H., Lile J. D., Bektesh S., Collins F. GDNF: A glial cell line-derived neurotrophic factor for midbrain dopaminergic neurons. Science. 260:1993;1130-1132.
18. Lin H. Y., Wang X-F., Ng-Eaton E., Weinberg R. A., Lodish H. F. Expression cloning of the TGF-β type II receptor, a functional transmembrane serine/threonine kinase. Cell. 68:1992;775-785.
19. Blaber M., Isackson P. J., Bradshaw R. A. The characterization of recombinant mouse glandular kallikreins fromE. coli. Proteins: Struct. Funct. Genet. 7:1990;280-290.
20. Chalfie M., Tu Y. T., Euskirchen G., Ward W. W., Prasher D. C. Green fluorescent protein as a marker for gene expression. Science. 263:1994;802-805.
21. Liu J., Walsh C. T. Peptidyl-prolylcistransE. coli: Proc. Natl. Acad. Sci. USA. 87:1990;4028-4032.
22. Mascarenhas, D. 1995, Methods and Vectors for Over-expression of Ubiquitin Fusion Proteins in Host Cells.
23. Bachmann B. J. Neidhardt F. C. Derivations and genotypes of some mutant derivatives ofE. coli. Escherichia coliSalmonella. 1996;2460-2488 ASM International, Materials Park.
24. Studier F. W., Moffatt B. A. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189:1986;113-130.
25. Squires C. H., Childs J., Eisenberg S. P., Polverini P. J., Sommer A. Production and characterization of human basic fibroblast growth factor fromE. coli. J. Biol. Chem. 263:1988;16297-16302.
26. Meacock P. A., Cohen S. N. Partitioning of bacterial plasmids during cell division: Acis. Cell. 20:1980;529-542.
27. Watkins B. A., Davis A. E., Cocchi F., Reitz Jr. A rapid method for site-specific mutagenesis using larger plasmids as templates. BioTechniques. 15:1993;700-704.
28. Firth S. M., Baxter R. C. The role of glycosylation in the action of IGFBP-3. Prog. Growth Fact. Res. 6:1995;223-229.
29. Schein C. H. Solubility and secretability. Curr. Opin. Biotechnol. 4:1993;456-461.
30. Wulfing C., Rappuoli R. Efficient production of heat-labile enterotoxin mutant proteins by overexpression of dsbA in a degP-deficientE. coli. Arch. Microbiol. 167:1997;280-283.
31. Rietsch A., Belin D., Martin N., Beckwith J. Anin vivoEscherichia coli. Proc. Natl. Acad. Sci. USA. 93:1996;13048-13053.
32. Wulfing C., Pluckthun A. Correctly folded T-cell receptor fragments in the periplasm ofEscherichia coli. J. Mol. Biol. 242:1994;655-669.
33. Yamanaka H., Kameyama M., Baba T., Fulii Y., Okamoto K. Maturation pathway ofE. coli. J. Bacteriol. 176:1994;2906-2913.
34. Larimer F. W., Soper T. S. Overproduction of Anabaena 7120 ribulose-bisphosphate carboxylase/oxygenase inE. coli. Gene. 126:1993;85-92.
35. Ashiuchi M., Yoshimura T., Kitamura T., Kawata Y., Nagai J., Gorlatov S., Esaki N., Soda K. In vivoE. coli. J. Biochem. (Tokyo). 117:1995;495-498.
36. Collins-Racie L. A., McColgan J. M., Grant K. L., DiBlasio-Smith E. A., McCoy J. M., LaVallie E. R. Production of recombinant bovine enterokinase catalytic subunit inEscherichia coli. Bio/Technology. 13:1995;982-987.
37. Buell G., Schulz M.-F., Selzer G., Chollet A., Movva N. R., Semon D., Escanez S., Kawashima E. Optimizing the expression inE. coli. Nucleic Acids Res. 13:1985;1923-1938.
38. Kain S. R., Adams M., Kondepudi A., Yang T.-T., Ward W. W., Kitts P. Green fluorescent protein as a reporter of gene expression and protein localization. BioTechniques. 19:1995;650-655.