The use of elements of the E. coli Ntr-system for the design of an optimized recombinant expression system for high cell density cultivations

Journal of Biotechnology

Volumn 75, Issue 2-3, 1999, Pages 241-250

  Schroeckh, Volker ^a   Wenderoth, Rolf ^a   Kujau, Marian ^a   Knüpfer, Uwe ^a   Riesenberg, Dieter ^a  

^a HANS KNÖLL INSTITUTE   (Germany)

Author keywords

High cell density cultivation; Inducible promoter; Nitrogen limitation; NtrC

Indexed keywords

ANTIBODIES; CELLS; ENZYMES; GENES; GLUCOSE; NITROGEN; RNA;

GLUTAMINE SYNTHETASE; HIGH CELL DENSITY CULTIVATION;

ESCHERICHIA COLI;

ARTICLE; BACTERIUM CULTURE; CELL DENSITY; ENZYME BINDING; ESCHERICHIA COLI; GENE EXPRESSION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION;

ANTIBODIES; BACTERIAL PROTEINS; BASE SEQUENCE; CELL DIVISION; CHLORAMPHENICOL O-ACETYLTRANSFERASE; DNA-BINDING PROTEINS; DNA-DIRECTED RNA POLYMERASES; EPIDERMAL GROWTH FACTOR; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GLUTAMATE-AMMONIA LIGASE; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; NITROGEN; PII NITROGEN REGULATORY PROTEINS; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS; RNA POLYMERASE SIGMA 54; SIGMA FACTOR; TRANS-ACTIVATORS; TRANSCRIPTION FACTORS;

EID: 0032863415     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(99)00167-4     Document Type: Article

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