Control of cloned gene expression by promoter inversion in vivo: construction of improved vectors with a multiple cloning site and the ptac promoter

Gene

Volumn 56, Issue 1, 1987, Pages 145-151

  Hasan, Noaman ^a   Szybalski, Waclaw ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Escherichia coli host; galactokinase; Int function; N nut mediated antitermination of transcription; phage att site; plasmid; Recombinant DNA

Indexed keywords

ARTICLE; BACTERIOPHAGE LAMBDA; CHROMOSOME INVERSION; DNA VECTOR; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETICS; MOLECULAR CLONING; PLASMID; PROMOTER REGION; VIRAL PHENOMENA AND FUNCTIONS;

ATTACHMENT SITES (MICROBIOLOGY); BACTERIOPHAGE LAMBDA; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETIC VECTORS; INVERSION (GENETICS); PLASMIDS; PROMOTER REGIONS (GENETICS); SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0023464008     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(87)90167-3     Document Type: Article

References (16)

1. A molecular map of coliphage lambda
2. Complete annotated lambda sequence
3. The tac promoter a functional hybrid derived from the trp and lac promoters
4. Boundaries of the nutL anti-terminator of coliphage lambda and effects of mutations in the spacer region between boxA and boxB
5. Effect of the promoter structure on the nutL transcription antitermination function
6. A novel multistep method for generating precise unidirectional deletions using BspMl, a class-IIS restriction enzyme
7. The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation
8. A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase
9. The extent of DNA sequence required for a functional bacterial attachment site of phage lambda
10. Integration and excision of bacteriophage gl: The mechanism of conservative site specific recombination
11. Control of cloned gene expression by promoter inversion in vivo: Construction of the heat-pulse-activated att-nutL-p-att-N module
12. Construction and analysis of in vitro activity of E. coli promoter hybrids and promoter mutants that alter the -35 to -10 spacing
13. Plasmids containing many tandem copies of a synthetic lactose operator
14. Modular structure of the nut and qut anti-terminators of transcription. Interactions between control elements of phage lambda and construction of novel regulatory circuits