A modular set of prokaryotic and eukaryotic expression vectors

Analytical Biochemistry

Volumn 277, Issue 1, 2000, Pages 109-120

  Melcher, Karsten ^a  

^a GOETHE UNIVERSITY   (Germany)

Author keywords

ADH1 promoter; GAL genes; GAL1 promoter; GST; Hexahistidine; T7 promoter; TEV protease

Indexed keywords

AMINO ACIDS; BIOACTIVITY; CLONE CELLS; ESCHERICHIA COLI; GLYCOSYLATION; PRODUCT DESIGN; PURIFICATION; VECTORS; VIRUSES; YEAST;

ADH1 PROMOTER; GAL1 PROMOTER; HEXAHISTIDINE; T7 PROMOTER; TEV PROTEASE;

RECOMBINANT PROTEINS;

GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; EUKARYOTE; EXPRESSION VECTOR; GENE EXPRESSION; PRIORITY JOURNAL; PROKARYOTE; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; REGULATORY MECHANISM; REGULON; SACCHAROMYCES CEREVISIAE;

ESCHERICHIA COLI; EUKARYOTA; NICOTIANA TABACUM; PROKARYOTA; SACCHAROMYCES CEREVISIAE; TOBACCO ETCH VIRUS;

EID: 0033988937     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1999.4383     Document Type: Article

References (20)

1. J.W. Jarvik C.A. Telmer Epitope tagging Annu. Rev. Genet. 32 1998 601 618
2. D.B. Smith K.S. Johnson Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S -transferase Gene 67 1988 31 40
3. D.A. Fancy K. Melcher S.A. Johnston T. Kodadek New chemistry for the study of multiprotein complexes: The six-histidine tag as receptor for a protein crosslinking reagent Chem. Biol. 3 1996 551 559
4. IIs are integral components of the SAGA complex required for nucleosome acetylation and transcriptional stimulation Cell 94 1998 45 53
5. D.A. Polayes A. Goldstein G. Ward A.J. Hughes TEV protease, recombinant: A site-specific protease for efficient cleavage of affinity tags from expressed proteins Focus 16 1994 2 5
6. W.G. Dougherty T.D. Parks S.M. Cary J.F. Bazan R. Fletterick Characterization of the catalytic residues of the tobacco etch virus 49-kDa proteinase Virology 172 1989 302 310
7. T.D. Parks K.K. Leuther E.D. Howard S.A. Johnston W.G. Dougherty Release of proteins and peptides from fusion proteins using a recombinant plant virus proteinase Anal. Biochem. 216 1994 413 417
8. W.G. Dougherty J.C. Carrington S.M. Cary T.D. Parks Biochemical and mutational analysis of a plant virus polyprotein cleavage site EMBO J. 7 1988 1281 1287
9. K. Melcher F.K. Zimmermann K.-D. Entian Yeast Sugar Metabolism 1997 Technomic Publishing Lancaster 235 269
10. Y. Wu R.J. Reece M. Ptashne Quantitation of putative activator-target affinities predicts transcriptional activating potentials EMBO J. 15 1996 3951 3963
11. K. Melcher S.A. Johnston GAL4 interacts with TATA binding protein and coactivators Mol. Cell. Biol. 15 1995 2839 2848
12. F.W. Studier A.H. Rosenberg J.J. Dunn J.W. Dubendorf Use of T7 RNA polymerase to direct the expression of cloned genes Methods Enzymol. 185 1990 60 89
13. K.K. Leuther S.A. Johnston Nondissociation of GAL4 and GAL80 in vivo after galactose induction Science 256 1992 1333 1335
14. T. Vernet D. Dignard D. Thomas A family of yeast expression vectors containing the phage f1 intergenic region Gene 52 1987 225 233
15. P. Hovland J. Flick M. Johnston R.A. Sclafani Galactose as a gratuitous inducer of GAL gene expression in yeasts growing on glucose Gene 83 1989 57 64
16. H. Ito Y. Fukuda K. Murata A. Kimura Transformation of intact yeast cells treated with alkali cations J. Bacteriol. 153 1983 163 168
17. F. Sherman G.R. Fink J.B. Hicks Laboratory Course Manual for Methods in Yeast Genetics 1986 Cold Spring Harbor Laboratory Press Cold Spring Harbor
18. M.A. Blanar W.J. Rutter Interaction cloning: Identification of a helix-loop-helix zipper protein that interacts with c-Fos Science 256 1992 1014 1018
19. E. Erhart C.P. Hollenberg The presence of defective LEU 2 gene on 2 μ DNA recombinant plasmids of Saccharomyces cerevisiae is responsible for curing and high copy number J. Bacteriol. 156 1983 625 635
20. L. Joshua-Tor H.E. Xu S.A. Johnston D.C. Rees Crystal structure of a conserved protease that binds DNA: The bleomycin hydrolase, Gal6 Science 269 1995 945 950