In vitro protein display in drug discovery

Current Opinion in Drug Discovery and Development

Volumn 4, Issue 2, 2001, Pages 198-204

  Lohse, P A ^a   Wright, M C ^a  

^a Phylos Inc   (United States)

Author keywords

In vitro selection; mRNA protein fusion; Phage display; PROfusion ; Protein display; Protein evolution; Ribosome display

Indexed keywords

PEPTIDE LIBRARY;

DNA LIBRARY; DNA TEMPLATE; DRUG RESEARCH; EPITOPE MAPPING; MUTAGENESIS; PHAGE DISPLAY; POLYMERASE CHAIN REACTION; PROTEIN NUCLEIC ACID INTERACTION; REVIEW; RIBOSOME;

ANIMALS; HUMANS; PEPTIDE LIBRARY; SEQUENCE ANALYSIS, PROTEIN; TECHNOLOGY, PHARMACEUTICAL;

EID: 0035071412     PISSN: 13676733     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (46)

1. Filamentous fusion phage: Novel expression vectors that display cloned antigens on the virion surface
2. Phage displayed peptide libraries
3. Phage display of combinatorial peptide and protein libraries and their applications in biology and chemistry
4. Phage-display technology - Finding a needle in a vast molecular haystack
5. Biosynthetic polypeptide libraries
6. In vitro selection methods for screening of peptide and protein libraries
7. Totally in vitro protein selection using mRNA-protein fusions and ribosome display
8. In vitro display technologies - New tools for drug discovery
9. STABLE: Protein-DNA fusion system for screening of combinatorial protein libraries in vitro
10. RNA-peptide fusions for the in vitro selection of peptides and proteins
11. In vitro virus: Bonding of mRNA bearing puromycin at the 3′-terminal end to the C-terminal end of its encoded protein on the ribosome in vitro
12. Ribosome display: An in vitro method for selection and evolution of antibodies from libraries
13. An in vitro polysome display system for identifying ligands from very large peptide libraries
14. Selection of a human anti-progesterone antibody fragment from a transgenic mouse library by ARM ribosome display
15. Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display
16. Comparison of Escherichia coli and rabbit reticulocyte ribosome display systems
17. Constructing high complexity synthetic libraries of long ORFs using in vitro selection
18. Optimized synthesis of RNA-protein fusions for in vitro protein selection
19. Psoralen photo-crosslinked mRNA-puromycin conjugates: A novel template for the rapid and facile preparation of mRNA-protein fusions
20. An efficient synthetic strategy for the preparation of nucleic acid-encoded peptide and protein libraries for in vitro evolution protocols
21. cDNA-protein fusions: Covalent protein-gene conjugates for the in vitro selection of peptides and proteins
22. Man-made enzymes - From design to in vitro compartmentalization
23. A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae
24. Efficient phage display of polypeptides fused to the carboxy-terminus of the M13 gene-3 minor coat protein
25. Phage display of cDNA repertoires: The pVI display system and its applications for the selection of immunogenic ligands
26. Display cloning: Functional identification of natural product receptors using cDNA-phage display
27. Yeast surface display for directed evolution of protein expression, affinity, and stability
28. PROfusion: Genetically tagged proteins for functional proteomics and beyond
29. Peptides as tools in drug discovery
30. Epitope discovery using monoclonal antibodies and phage peptide libraries
31. Phage antibodies: Filamentous phage displaying antibody variable domains
32. Natural and designer binding sites made by phage display technology
33. Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library
34. Recent progress in biomolecular engineering
35. Creating and engineering human antibodies for immunotherapy
36. Exploiting recombination in single bacteria to make large phage antibody libraries
37. Selectable in vivo recombination to increase antibody library size - An improved phage display vector system
38. Antibody arrays for high-throughput screening of antibody-antigen interactions
39. By-passing selection: Direct screening for antibody-antigen interactions using protein arrays
40. Fine affinity discrimination by yeast surface display and flow cytometry
41. Quantitative analysis of the effect of the mutation frequency on the affinity maturation of single chain Fv antibodies
42. Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity
43. Tailoring in vitro evolution for protein affinity or stability
44. The fibronectin type III domain as a scaffold for novel binding proteins
45. Printing proteins as microarrays for high-throughput function determination
46. Large-scale functional analysis using peptide or protein arrays