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Gao C, Lin CH, Lo CHL, Mao S, Wirsching P, Lerner RA, Janda KD Making chemistry selectable by linking it to infectivity. Proc Natl Acad Sci USA. 94:1997;11777-11782. There are still significant technical barriers to using phage display technology for selection of chemical activity. In this paper, three strategies for this process are described in detail. The bottom line here is that the technology is still in its infancy, but that there is great promise for practical applications in this field in the future.
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47
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Structural plasticity in a remodeled protein-protein interface
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Phage display is a powerful tool for understanding protein-protein interactions. In this work, the authors create a large cavity at a protein-protein interface by deleting a critical tryptophan residue. Selection of a pentamutant by phage display that fills the cavity largely restores binding affinity.
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Atwell S, Ultsch M, De Vos AM, Wells JA Structural plasticity in a remodeled protein-protein interface. Science. 278:1997;1125-1128. Phage display is a powerful tool for understanding protein-protein interactions. In this work, the authors create a large cavity at a protein-protein interface by deleting a critical tryptophan residue. Selection of a pentamutant by phage display that fills the cavity largely restores binding affinity.
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Atwell, S.1
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Selection of a cyclic nonapeptide inhibitor to alpha-chymotrypsin using a phage display peptide library
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Krook M, Lindbladh C, Eriksen JA, Mosbach K Selection of a cyclic nonapeptide inhibitor to alpha-chymotrypsin using a phage display peptide library. Mol Diversity. 3:1998;149-159.
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Selection of peptides that bind to plasminogen activator inhibitor 1 (PAI-1) using random peptide phage-display libraries
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55
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A peptide isolated by phage display binds to ICAM-1 and inhibits binding to LFA-1
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Screening a library of phage displayed peptides identifies human Bcl-2 as a taxol-binding protein
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Rodi, D.J.1
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61
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Molecular approach to find target(s) for oligoclonal bands in multiple sclerosis
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By screening a random peptide library for affinity to cerebrospinal fluid IgGs from multiple sclerosis patients, the authors identified a sequence motif corresponding to the Epstein-Barr virus (EBV) nuclear antigen. This result suggests that the immune response to this particular antigen in EBV may be a defining event in the development of MS.
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Rand KH, Houck H, Denslow ND, Heilman KM Molecular approach to find target(s) for oligoclonal bands in multiple sclerosis. J Neurol Neurosurg Psychiatry. 65:1998;48-55. By screening a random peptide library for affinity to cerebrospinal fluid IgGs from multiple sclerosis patients, the authors identified a sequence motif corresponding to the Epstein-Barr virus (EBV) nuclear antigen. This result suggests that the immune response to this particular antigen in EBV may be a defining event in the development of MS.
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Rand, K.H.1
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Choo Y, Klug A Toward a code for the interactions of zinc fingers with DNA: selection of randomized fingers displayed on phage. Proc Natl Acad Sci USA. 91:1994;11163-11167.
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Smith GP, Patel SU, Windass JD, Thornton JM, Winter G, Griffiths AD Small binding proteins selected from a combinatorial repertoire of knottins displayed on phage. J Mol Biol. 277:1998;317-332.
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64
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Simultaneous display of different peptides on the surface of filamentous bacteriophage
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The potential to display two distinct peptides or proteins has immediate and obvious application to the construction of targeted pharmaceuticals based on the phage structure.
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Malik P, Perham RN Simultaneous display of different peptides on the surface of filamentous bacteriophage. Nucleic Acids Res. 25:1997;915-916. The potential to display two distinct peptides or proteins has immediate and obvious application to the construction of targeted pharmaceuticals based on the phage structure.
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Malik, P.1
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