The glucocorticoid receptor inhibits NFκB by interfering with serine-2 phosphorylation of the RNA polymerase II carboxy-terminal domain

Genes and Development

Volumn 14, Issue 18, 2000, Pages 2314-2329

  Nissen, R M ^a   Yamamoto, K R ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Anti inflammation; Chromatin; Glucocorticoid receptor; Intracellular receptor; RelA; Transcriptional repression

Indexed keywords

GLUCOCORTICOID RECEPTOR; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INTERCELLULAR ADHESION MOLECULE 1; INTERLEUKIN 8; RNA POLYMERASE II; SERINE DERIVATIVE; TUMOR NECROSIS FACTOR ALPHA; ZINC;

ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; COMPLEX FORMATION; CONTROLLED STUDY; DIMERIZATION; DNA BINDING; ENZYME SUBUNIT; GENE ACTIVATION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; METAL BINDING; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; TRANSCRIPTION REGULATION;

CELL LINE; HUMAN; I-KAPPA B; INTERCELLULAR ADHESION MOLECULE-1; INTERLEUKIN-8; NF-KAPPA B; PHOSPHORYLATION; PRECIPITIN TESTS; PROMOTER REGIONS (GENETICS); PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, GLUCOCORTICOID; REPRESSOR PROTEINS; RESPONSE ELEMENTS; RNA POLYMERASE II; SERINE; SUPPORT, U.S. GOV'T, P.H.S.; TUMOR NECROSIS FACTOR; ZINC;

EID: 0034665748     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: 10.1101/gad.827900     Document Type: Article

References (131)

1. Ligand-induced differentiation of glucocorticoid receptor (GR) trans-repression and transactivation: Preferential targetting of NF-κB and lack of I-κB involvement
2. The molecular mechanism of mitotic inhibition of TFIIH is mediated by phosphorylation of CDK7
3. Role for N-CoR and histone deacetylase in Sin3-mediated transcriptional repression
4. Extensive homology among the largest subunits of eukaryotic and prokaryotic RNA polymerases
5. A transcriptional activating region with two contrasting modes of protein interaction
6. An essential component of a C-terminal domain phosphatase that interacts with transcription factor IIF in Saccharomyces cerevisiae
7. Immunosuppression by glucocorticoids: Inhibition of NF-κ B activity through induction of I κ B synthesis
8. Mad proteins contain a dominant transcription repression domain
9. Activation of DNA-binding activity in an apparently cytoplasmic precursor of the NF-κ B transcription factor
10. I κ B: A specific inhibitor of the NF-κ B transcription factor
11. The NF-κ B and I κ B proteins: New discoveries and insights
12. Genetic analysis of the repetitive carboxyl-terminal domain of the largest subunit of mouse RNA polymerase II
13. Going the distance: A current view of enhancer action
14. The histone acetylase PCAF is a nuclear receptor coactivator
15. Cytoplasmic retention, DNA binding and processing of the NF-κ B p50 precursor are controlled by a small region in its C-terminus
16. Transcription-independent phosphorylation of the RNA polymerase II C-terminal domain (CTD) involves ERK kinases (MEK1/2)
17. Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern
18. Glucocorticoid-mediated repression of NFκB activity in endothelial cells does not involve induction of IκBα synthesis
19. Negative cross-talk between RelA and the glucocorticoid receptor: A possible mechanism for the antiinflammatory action of glucocorticoids
20. Crystal structure of p50/p65 heterodimer of transcription factor NF- κB bound to DNA
21. Nuclear receptor coactivator ACTR is a novel histone acetyltransferase and forms a multimeric activation complex with P/CAF and CBP/p300
22. Signal-induced site-specific phosphorylation targets I κ B α to the ubiquitin-proteasome pathway
23. Binding of liganded vitamin D receptor to the vitamin D receptor interacting protein coactivator complex induces interaction with RNA polymerase II holoenzyme
24. A protein phosphatase functions to recycle RNA polymerase II
25. KIN28 encodes a C-terminal domain kinase that controls mRNA transcription in Saccharomyces cerevisiae but lacks cyclin-dependent kinase-activating kinase (CAK) activity
26. Sex hormones and glucocorticoids: Interactions with the immune system
27. Structure and specificity of nuclear receptor-coactivator interactions
28. Glucocorticoid-mediated repression of nuclear factor-κB-dependent transcription involves direct interference with transactivation
29. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei
30. Stress, corticotropin-releasing hormone, glucocorticoids, and the immune/inflammatory response: Acute and chronic effects
31. The p23 molecular chaperones act at a late step in intracellular receptor action to differentially affect ligand efficacies
32. Structure of NF-κ B p50 homodimer bound to a κ B site
33. NF-κ B and Rel proteins: Evolutionarily conserved mediators of immune responses
34. Glucocorticoid receptor mutants that are constitutive activators of transcriptional enhancement
35. Yeast Gcn5 functions in two multisub-unit complexes to acetylate nucleosomal histones: Characterization of an Ada complex and the SAGA (Spt/Ada) complex
36. Histone acetylation in chromatin structure and transcription
37. Transcription factor TFIIH is required for promoter melting in vivo
38. Essential involvement of interleukin-8 (IL-8) in acute inflammation
39. Histone deacetylase activity is required for full transcriptional repression by mSin3A
40. I κB α-independent downregulation of NF-κB activity by glucocorticoid receptor
41. A signature motif in transcriptional co-activators mediates binding to nuclear receptors
42. A complex containing N-CoR, mSin3 and histone deacetylase mediates transcriptional repression
43. Temporal regulation of RNA polymerase II by Srb10 and Kin28 cyclin-dependent kinases
44. Distinct roles for CTD Ser-2 and Ser-5 phosphorylarion in the recruitment and allos eric activation of mammalian mRNA capping enzyme
45. Stabilization of cationic liposome-plasmid DNA complexes by polyamines and poly(ethylene glycol)-phospholipid conjugates for efficient in vivo gene delivery
46. Mapping the HSP90 binding region of the glucocorticoid receptor
47. Three amino acid substitutions selectively disrupt the activation but not the repression function of the glucocorticoid receptor N terminus
48. Negative interaction between the RelA(p65) subunit of NF-κB and the progesterone receptor
49. Mechanism of ATP-dependent promoter melting by transcription factor IIH
50. An RNA polymerase II holoenzyme responsive to activators
51. Interference between pathway-specific transcription factors: Glucocorticoids antagonize phorbol ester-induced AP-1 activity without altering AP-1 site occupation in vivo
52. p300 and estrogen receptor cooperatively activate transcription via differential enhancement of initiation and reinitiation
53. Synergistic transcriptional activation of the IL-8 gene by NF-κ B p65 (RelA) and NF-IL-6
54. Binding of TBP to promoters in vivo is stimulated by activators and requires Pol II holoenzyme
55. Histone deacetylases associated with the mSin3 corepressor mediate mad transcriptional repression
56. Promoter analysis of the gene encoding the I κ B-α/MAD3 inhibitor of NF-κ B: Positive regulation by members of the rel NF-κ B family
57. Transcriptional activation independent of TFIIH kinase and the RNA polymerase II mediator in vivo
58. CTD kinase large subunit is encoded by CTK1, a gene required for normal growth of Saccharomyces cerevisiae
59. Allosteric effects of DNA on transcriptional regulators
60. Influence of a steroid receptor DNA-binding domain on transcriptional regulatory functions
61. The sensitivity of RNA polymerase II in elongation complexes to C-terminal domain phosphatase
62. A new function for the C-terminal zinc finger of the glucocorticoid receptor. Repression of RelA transactivation
63. Repression of TFIIH transcriptional activity and TFIIH-associated cdk7 kinase activity at mitosis
64. Interaction of GAL4 and GAL80 gene regulatory proteins in vitro
65. Crystallographic analysis of the interaction of the glucocorticoid receptor with DNA
66. GAL4 interacts with TATA-binding protein and coactivators
67. IKK-1 and IKK-2: Cytokine-activated IκB kinases essential for NF-κB activation
68. Regulatory crosstalk at composite response elements
69. The TAF(II)250 subunit of TFIID has histone acetyltransferase activity
70. A role for the TFIIH XPB DNA helicase in promoter escape by RNA polymerase II
71. Molecular analysis of the inhibition of interleukin-8 production by dexamethasone in a human fibrosarcoma cell line
72. Novel mechanism of glucocorticoid-mediated gene repression. Nuclear factor-κ B is target for glucocorticoid-mediated interleukin 8 gene repression
73. Composite co-activator ARC mediates chromatin-directed transcriptional activation
74. Nuclear receptor repression mediated by a complex containing SMRT, mSin3A, and histone deacetylase
75. Effect of dexamethasone on interleukin-1β-(IL-1β)-induced nuclear factor-κB (NF-κB) and κB-dependent transcription in epithelial cells
76. Functional redundancy and structural polymorphism in the large subunit of RNA polymerase II
77. Phosphorylation of RNA polymerase II C-terminal domain and transcriptional elongation
78. Analysis of chromatin structure by in vivo formaldehyde cross-linking
79. Relationship between the product of the Drosophila ultraspiracle locus and the vertebrate retinoid X receptor
80. The chromatin-specific transcription elongation factor FACT comprises human SPT16 and SSRP1 proteins
81. Mutual transcriptional interference between RelA and androgen receptor
82. Enhanced processivity of RNA polymerase II triggered by Tat-induced phosphorylation of its carboxy-terminal domain
83. Vires infection leads to localized hyperacetylation of histones H3 and H4 at the IFN-β promoter
84. Yeast carboxyl-terminal domain kinase I positively and negatively regulates RNA polymerase II carboxyl-terminal domain phosphorylation
85. Molecular determinants of nuclear receptor-corepressor interaction
86. Two signals mediate hormone-dependent nuclear localization of the glucocorticoid receptor
87. A movable and regulable inactivation function within the steroid binding domain of the glucocorticoid receptor
88. P-TEFb, a cyclin-dependent kinase controlling elongation by RNA polymerase II
89. The DRIP complex and SRC-1/p160 coactivators share similar nuclear receptor binding determinants but constitute functionally distinct complexes
90. Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex
91. Physical association and functional antagonism between the p65 subunit of transcription factor NF-κ B and the glucocorticoid receptor
92. Down-modulation of interleukin-6 gene expression by 17 β-estradiol in the absence of high affinity DNA binding by the estrogen receptor
93. The role of general initiation factors in transcription by RNA polymerase II
94. Functional dissection of the hormone and DNA binding activities of the glucocorticold receptor
95. The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Spl
96. GAL4-VP16 is an unusually potent transcriptional activator
97. Role of transcriptional activation of I κ B α in mediation of immunosuppression by glucocorticoids
98. Characterization of mechanisms involved in transrepression of NF-κ B by activated glucocorticoid receptors
99. Mutations in the glucocorticoid receptor zinc finger region that distinguish interdigitated DNA binding and transcriptional enhancement activities
100. Signal-induced degradation of I κ B α requires site-specific ubiquitination
101. Interaction of the COOH-terminal transactivation domain of p65 NF-κ B with TATA-binding protein, transcription factor IIB, and coactivators
102. Transcriptionally repressed germ cells lack a subpopulation of phosphorylated RNA polymerase II in early embryos of Caenorhabditis elegans and Drosophila melanogaster
103. The Gal3p-Gal80p-Gal4p transcription switch of yeast: Gal3p destabilizes the Gal80p-Ga14p complex in response to galactose and ATP
104. Transcriptional regulation of a pair-rule stripe in Drosophila
105. Regulation of cytomegalovirus gene expression: Alpha and beta promoters are trans activated by viral functions in permissive human fibroblasts
106. Intracellular receptors use a common mechanism to interpret signaling information at response elements
107. Repression of the interleukin-6 promoter by estrogen receptor is mediated by NF-κ B and C/EBP β
108. NF-κ B controls expression of inhibitor I κ B α: Evidence for an inducible autoregulatory pathway
109. NAT, a human complex containing Srb polypeptides that functions as a negative regulator of activated transcription
110. The multiple roles of transcription/repair factor TFIIH
111. A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p
112. Genetic requirements for PIE-1 localization and inhibition of gene expression in the embryonic germ lineage of Caenorhabditis elegans
113. Functional dissection of VP16, the trans-activator of herpes simplex vires immediate early gene expression
114. Characterization of the residues phosphorylated in vitro by different C-terminal domain kinases
115. Molecular mechanisms of action of steroid/thyroid receptor superfamily members
116. Glucocorticoid-mediated repression of intercellular adhesion molecule-expression in human monocytic and bronchial epithelial cell lines
117. 12-O-tetradecanoylphorbol-13-acetate- and tumor necrosis factor α-mediated induction of intercellular adhesion molecule-1 is inhibited by dexamethasone. Functional analysis of the human intercellular adhesion molecular-1 promoter
118. The coactivator TIF2 contains three nuclear receptor-binding motifs and mediates transactivation through CBP binding-dependent and -independent pathways
119. Construction and analysis of yeast RNA polymerase II CTD deletion and substitution mutations
120. Distinct domains of the RelA NF-κB subunit are required for negative cross-talk and direct interaction with the glucocorticoid receptor
121. A dual mechanism mediates repression of NF-κB activity by glucocorticoids
122. IκB kinase-β: NF-κB activation and complex formation with IκB kinase-α and NIK
123. Multilayered control of intracellular receptor function
124. Building transcriptional regulatory complexes: Signals and surfaces
125. TAFII105 mediates activation of anti-apoptotic genes by NF-κB
126. Galactose-dependent reversible interaction of Ga13p with Ga180p in the induction pathway of Ga14p-activated genes of Saccharomyces cerevisiae
127. Coupling of cell identity to signal response in yeast: Interaction between the α 1 and STE12 proteins
128. Common themes in assembly and function of eukaryotic transcription complexes
129. Histone deacetylases and SAP18, a novel polypeptide, are components of a human Sin3 complex
130. Phosphorylation of NF-κ B p65 by PKA stimulates transcriptional activity by promoting a novel bivalent interaction with the coactivator CBP/p300