IκB kinase-β: NF-κB activation and complex formation with IκB kinase-α and NIK

Science

Volumn 278, Issue 5339, 1997, Pages 866-869

  Woronicz, John D ^a   Gao, Xiong ^a   Cao, Zhaodan ^a   Rothe, Mike ^a   Goeddel, David V ^a  

^a AMGEN INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOKINE; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INTERLEUKIN 1; PROTEIN KINASE; SERINE; TUMOR NECROSIS FACTOR; CHUK PROTEIN, HUMAN; I KAPPA B KINASE; IKBKB PROTEIN, HUMAN; IKBKE PROTEIN, HUMAN; NF KAPPA B KINASE; NF-KAPPA B KINASE; PROTEIN SERINE THREONINE KINASE;

AMINO ACID SEQUENCE; ARTICLE; ENZYME ACTIVATION; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; IMMUNOBLOTTING; INFLAMMATION; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; TRANSCRIPTION INITIATION; CELL LINE; GENETIC TRANSFECTION; HELA CELL; METABOLISM; MOLECULAR GENETICS; PHOSPHORYLATION; REPORTER GENE; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; CELL LINE; CYTOKINES; ENZYME ACTIVATION; GENES, REPORTER; HELA CELLS; HUMANS; I-KAPPA B KINASE; MOLECULAR SEQUENCE DATA; NF-KAPPA B; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; SEQUENCE HOMOLOGY, AMINO ACID; TRANSFECTION;

EID: 0030611595     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.278.5339.866     Document Type: Article

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26. Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
27. (1-311) wild-type and mutant expression plasmids are described in (11). The amounts of purified Flag-IKK proteins used in the kinase reactions were determined by immunoblotting with polyclonal antibodies to Flag (Santa Cruz Biotechnology). Only experiments with comparable amounts of Flag-IKK proteins were taken into consideration.
28. We thank H.-B. Shu for constructing the IKK-β deletion mutants; K. Williamson for DNA sequencing; C. Régnier for expression vectors; and P. Baeuerle, C. Kirschning, L. Ling, and C. Régnier for helpful discussions.