Protein and lipid requirements for endocytosis

Annual Review of Genetics

Volumn 34, Issue , 2000, Pages 255-295

  D'Hondt, K ^a   Heese Peck, A ^a   Riezman, H ^a  

^a UNIVERSITY OF BASEL   (Switzerland)

Author keywords

Actin; Clathrin; Dynamin; EH domain; Endosome; Phosphoinositides; Sterol

Indexed keywords

ACTIN; DYNAMIN; MEMBRANE LIPID; MEMBRANE PROTEIN; PHOSPHATIDYLINOSITIDE; SPHINGOLIPID; STEROL DERIVATIVE;

ENDOCYTOSIS; ENDOSOME; GENETIC SCREENING; HUMAN; INTERNALIZATION; MEMBRANE TRANSPORT; MEMBRANE VESICLE; PRIORITY JOURNAL; REVIEW;

ANIMALS; ENDOCYTOSIS; GENETIC SCREENING; LIPIDS; PROTEINS;

ANIMALIA;

EID: 0034516332     PISSN: 00664197     EISSN: None     Source Type: Book Series    
DOI: 10.1146/annurev.genet.34.1.255     Document Type: Review

References (271)

1. Tlg2p, a yeast syntaxin homolog that resides on the Golgi and endocytic structures
2. Essential role of the dynamin pleckstrin homology domain in receptor-mediated endocytosis
3. Binding of myosin I to membrane lipids
4. Defining protein interactions with yeast actin in vivo
5. The Src homology domain 3 (SH3) of a yeast type I myosin, Myo5p, binds to verprolin and is required for targeting to sites of actin polarization
6. Bound simian virus 40 translocates to caveolin-enriched membrane domains, and its entry is inhibited by drugs that selectively disrupt caveolae
7. Synergistic activation of dynamin GTPase by Grb2 and phosphoinositides
8. The END3 gene encodes a protein that is required for the internalization step of endocytosis and for actin cytoskeleton organization in yeast
9. Inhibition of clathrin-coated pit assembly by an Eps15 mutant
10. The ear of alpha-adaptin interacts with the COOH-terminal domain of the Eps 15 protein
11. The tyrosine kinase substrate eps15 is constitutively associated with the plasma membrane adaptor AP-2
12. AP-2/Eps15 interaction is required for receptor-mediated endocytosis
13. Molecular bases for the recognition of tyrosine-based sorting signals
14. Genetic and functional relationship between Rvsp, myosin and actin in Saccharomyces cerevisiae
15. Functions of lipid rafts in biological membranes
16. Sphingolipid organization in biomembranes: What physical studies of model membranes reveal
17. Cloning and characterization of LCB1, a Saccharomyces gene required for biosynthesis of the long-chain base component of sphingolipids
18. Phosphatidylinositol(3)-phosphate signaling mediated by specific binding to RING FYVE domains
19. The Dbl family of oncogenes
20. Lowering the cholesterol content of MA104 cells inhibits receptor-mediated transport of folate
21. The role of ARF and Rab GTPases in membrane transport
22. Ceramide as a modulator of endocytosis
23. Epsin is an EH-domain-binding protein implicated in clathrin-mediated endocytosis
24. The interaction of epsin and Eps15 with the clathrin adaptor AP-2 is inhibited by mitotic phosphorylation and enhanced by stimulation-dependent dephosphorylation in nerve terminals
25. Systematic mutational analysis of the cation-independent mannose 6-phosphate/insulin-like growth factor II receptor cytoplasmic domain. An acidic cluster containing a key aspartate is important for function in lysosomal enzyme sorting
26. Interaction in vivo and in vitro between the yeast fimbrin, SAC6P, and a polymerization-defective yeast actin (V266G and L267G)
27. The Rab5 effector EEA1 is a core component of endosome docking
28. Phosphatidylinositol-3-OH kinases are Rab5 effectors
29. In vivo analysis of the domains of yeast Rvs167p suggests Rvs167p function is mediated through multiple protein interactions
30. Direct targets of phosphoinositide 3-kinase products in membrane traffic and signal transduction
31. Phosphoinositides in membrane traffic
32. Essential role of phosphoinositide metabolism in synaptic vesicle recycling
33. Assembly of clathrin coats disrupts the association between Eps15 and AP-2 adaptors
34. A multispecificity syntaxin homologue, Vam3p, essential for autophagic and biosynthetic protein transport to the vacuole
35. Biochemistry, cell biology and molecular biology of lipids of Saccharomyces cerevisiae
36. The J.D. mutation in familial hypercholesterolemia: Amino acid substitution in cytoplasmic domain impedes internalization of LDL receptors
37. Complementation of growth factor receptor-dependent mitogenic signaling by a truncated type I phosphatidylinositol 4-phosphate 5-kinase
38. Cis- and trans-acting functions required for endocytosis of the yeast pheromone receptors
39. Phosphoinositides as regulators in membrane traffic
40. Endocytosis of GPI-anchored proteins in human lymphocytes: Role of glycolipid-based domains, actin cytoskeleton, and protein kinases
41. EH: A novel protein-protein interaction domain potentially involved in intracellular sorting
42. Sphingolipid functions in Saccharomyces cerevisiae: Comparison to mammals
43. Epsin binds to clathrin by associating directly with the clathrin-terminal domain. Evidence for cooperative binding through two discrete sites
44. Yeast actin-binding proteins: Evidence for a role in morphogenesis
45. Yeast endocytosis assays
46. The yeast multidrug transporter Pdr5 of the plasma membrane is ubiquitinated prior to endocytosis and degradation in the vacuole
47. An actin-binding protein of the Sla2/Huntingtin interacting protein 1 family is a novel component of clathrin-coated pits and vesicles
48. A role for myosin-I in actin assembly through interactions with Vrp1p, Bee1p, and the Arp2/3 complex
49. Role of beta-arrestin in mediating agonist-promoted G protein-coupled receptor internalization
50. Nef-mediated clathrin-coated pit formation
51. De novo formation of caveolae in lymphocytes by expression of VIP21-caveolin
52. A conserved proline-rich region of the Saccharomyces cerevisiae cyclase-associated protein binds SH3 domains and modulates cytoskeletal localization
53. Increased protein kinase or decreased PPA2A activity bypass sphingoid base requirement in endocytosis
54. Actin assembly plays a variable, but not obligatory role in receptor-mediated endocytosis in mammalian cells
55. A functional phosphatidylinositol 3,4,5-trisphosphate/phosphoinositide binding domain in the clathrin adaptor AP-2 alpha subunit. Implications for the endocytic pathway
56. Phosphoinositide-AP-2 interactions required for targeting to plasma membrane clathrin-coated pits
57. Arrestin function in G protein-coupled receptor endocytosis requires phosphoinositide binding
58. Ubiquitin lys63 is involved in ubiquitination of a yeast plasma membrane protein
59. FYVE fingers bind PtdIns(3)P
60. Role of type I myosins in receptor-mediated endocytosis in yeast
61. Endocytic internalization in yeast and animal cells: Similar and different
62. VPS21 controls entry of endocytosed and biosynthetic proteins into the yeast prevacuolar compartment
63. Pep12p is a multifunctional yeast syntaxin that controls entry of biosynthetic, endocytic and retrograde traffic into the prevacuolar compartment
64. Regulation of membrane trafficking: Structural insights from a Rab/effector complex
65. Beta-arrestin acts as a clathrin adaptor in endocytosis of the beta2-adrenergic receptor
66. Synthetic lethality screen identifies a novel yeast myosin I gene (MYO5): Myosin I proteins are required for polarization of the actin cytoskeleton
67. Actin microfilaments play a critical role in endocytosis at the apical but not the basolateral surface of polarized epithelial cells
68. Identification of a novel ubiquitin conjugation motif, required for ligand-induced internalization of the growth hormone receptor
69. Receptor-mediated endocytosis in the Caenorhabditis elegans oocyte
70. Acidic sphingomyelinase mediates entry of N. gonorrhoeae into nonphagocytic cells
71. Synaptojanin 1: Localization on coated endocytic intermediates in nerve terminals and interaction of its 170 kDa isoform with Eps15
72. Regulation of caveolin and caveolae by cholesterol in MDCK cells
73. Rho GTPases and the actin cytoskeleton
74. Vps9p is a guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport
75. Ceramide in the eukaryotic stress response
76. AP-2 recruitment to synaptotagmin stimulated by tyrosine-based endocytic motifs
77. A C-terminal di-leucine motif and nearby sequences are required for NH4(+)-induced inactivation and degradation of the general amino acid permease, Gap1p, of Saccharomyces cerevisiae
78. NP11, an essential yeast gene involved in induced degradation of Gap1 and Fur4 permeases, encodes the Rsp5 ubiquitin-protein ligase
79. Dynamin-mediated internalization of caveolae
80. Gettin' down with ubiquitin: Turning off cell-surface receptors, transporters and channels
81. Ubiquitination of a yeast plasma membrane receptor signals its ligand-stimulated endocytosis
82. Cytoplasmic tail phosphorylation of the alpha-factor receptor is required for its ubiquitination and internalization
83. Dynamin self-assembles into rings suggesting a mechanism for coated vesicle budding
84. Clathrin and adaptors
85. Ceramide in apoptosis-does it really matter?
86. Vectorial budding of vesicles by asymmetrical enzymatic formation of ceramide in giant liposomes
87. Two syntaxin homologues in the TGN/endosomal system of yeast
88. Synthetic-lethal interactions identify two novel genes, SLA1 and SLA2, that control membrane cytoskeleton assembly in Saccharomyces cerevisiae
89. Membrane biology: Do glycolipid microdomains really exist?
90. Catabolite inactivation of the galactose transporter in the yeast Saccharomyces cerevisiae: Ubiquitination, endocytosis, and degradation in the vacuole
91. VPS21 encodes a rab5-like GTP binding protein that is required for the sorting of yeast vacuolar proteins
92. A novel Rab5 GDP/GTP exchange factor complexed to Rabaptin-5 links nucleotide exchange to effector recruitment and function
93. Clathrin functions in the absence of heterotetrameric adaptors and AP180-related proteins in yeast
94. Novel functions of clathrin light chains: Clathrin heavy chain trimerization is defective in light chain-deficient yeast
95. Splice variants of intersectin are components of the endocytic machinery in neurons and nonneuronal cells
96. A novel endocytosis signal related to the KKXX ER-retrieval signal
97. Looking at lipid rafts?
98. Phosphatidylinositol 3-kinase activity is required at a postendocytic step in platelet-derived growth factor receptor trafficking
99. Phosphatidylinositol 3-kinase activity is required for early endosome fusion
100. Phosphatidylinositol-4,5-bisphosphate is required for endocytic coated vesicle formation
101. Internalization of activated platelet-derived growth factor receptor-phosphatidylinositol-3′ kinase complexes: Potential interactions with the microtubule cytoskeleton
102. New twists for dynamin
103. Adaptors for clathrin-mediated traffic
104. The pleckstrin homology domains of dynamin isoforms require oligomerizafion for high affinity phosphoinositide binding
105. Lipids, lipid domains and lipid-protein interactions in endocytic membrane traffic
106. A lipid associated with the antiphospholipid syndrome regulates endosome structure and function
107. The ABC-transporter Ste6 accumulates in the plasma membrane in a ubiquitinated form in endocytosis mutants
108. The linker region of the ABC-transporter Ste6 mediates ubiquitination and fast turnover of the protein
109. Possible temperature-dependent blockage of synaptic vesicle recycling induced by a single gene mutation in Drosophila
110. Reversible blockage of membrane retrieval and endocytosis in the garland cell of the temperature-sensitive mutant of Drosophila melanogaster, shibirets1
111. Modulation of the arrestin-clathrin interaction in cells. Characterization of beta-arrestin dominant-negative mutants
112. Actin and fimbrin are required for the internalization step of endocytosis in yeast
113. The actin cytoskeleton is required for receptor-mediated endocytosis in mammalian cells
114. The beta2-adrenergic receptor/beta arrestin complex recruits the clathrin adaptor AP-2 during endocytosis
115. Direct involvement of yeast type I myosins in Cdc42-dependent actin polymerization
116. Dominant-negative inhibition of receptor-mediated endocytosis by a dynamin-1 mutant with a defective pleckstrin homology domain
117. ESR determination of membrane order parameter in yeast sterol mutants
118. Clathrin requirement for normal growth of yeast
119. A novel di-leucine motif and a tyrosine-based motif independently mediate lysosomal targeting and endocytosis of CD3 chains
120. Specific retrieval of the exocytic SNARE snc1p from early yeast endosomes
121. Beel, a yeast protein with homology to Wiscott-Aldrich syndrome protein, is critical for the assembly of cortical actin cytoskeleton
122. Induction of endocytic vesicles by exogenous C(6)-ceramide
123. Evidence for physical and functional interactions among two Saccharomyces cerevisiae SH3 domain proteins, an adenylyl cyclase-associated protein and the actin cytoskeleton
124. Catabolite inactivation of the yeast maltose transporter requires ubiquitin-ligase npi1/rsp5 and ubiquitin-hydrolase npi2/doa4
125. Novel genes involved in endosomal traffic in yeast revealed by suppression of a targeting-defective plasma membrane ATPase mutant
126. The Arp2/3 complex: A multifunctional actin organizer
127. Signaling to actin dynamics
128. The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex
129. A PEST-like sequence mediates phosphorylation and efficient ubiquitination of yeast uracil permease
130. Protein sorting by tyrosine-based signals: Adapting to the Ys and wherefores
131. Phosphoinositide lipids as signaling molecules: Common themes for signal transduction, cytoskeletal regulation, and membrane trafficking
132. Intracellular membrane fusion: SNAREs only?
133. Intracellular membrane fusion: SNAREs only?
134. Cholesterol-dependent retention of GPI-anchored proteins in endosomes
135. Oligomeric complexes link Rab5 effectors with NSF and drive membrane fusion via interactions between EEA1 and syntaxin 13
136. The I/LWEQ module: A conserved sequence that signifies F-actin binding in functionally diverse proteins from yeast to mammals
137. Dynamin: A molecular motor with pinchase action
138. The role of ubiquitin conjugation in glucose-induced proteolysis of Saccharomyces maltose permease
139. Endocytosis and molecular sorting
140. Endocytic vesicles move at the tips of actin tails in cultured mast cells
141. Oligomerization of VIP21-caveolin in vitro is stabilized by long chain fatty acylation or cholesterol
142. The yeast actin-related protein Arp2p is required for the internalization step of endocytosis
143. Cholesterol: Stuck in traffic
144. Specific sterols required for the internalization step of endocytosis in yeast
145. Endocytosis is required for the growth of vacuolar H(+)-ATPase-defective yeast: Identification of six new END genes
146. Viability of clathrin heavy-chain-deficient Saccharomyces cerevisiae is compromised by mutations at numerous loci: Implications for the suppression hypothesis
147. end5, end6, and end7: Mutations that cause actin delocalization and block the internalization step of endocytosis in Saccharomyces cerevisiae
148. VIP21/caveolin is a cholesterol-binding protein
149. The WASp homologue Las17p functions with the WIP homologue End5p/verprolin and is essential for endocytosis in yeast
150. Protein-protein interaction between the RVS161 and RVS167 gene products of Saccharomyces cerevisiae
151. Disruption of genes encoding subunits of yeast vacuolar H(+)-ATPase causes conditional lethality
152. Inhibition of the receptor-binding function of clathrin adaptor protein AP-2 by dominant-negative mutant mu2 subunit and its effects on endocytosis
153. Ligand-induced endocytosis of epidermal growth factor receptors that are defective in binding adaptor proteins
154. Dynamin at the neck of caveolae mediates their budding to form transport vesicles by GTP-driven fission from the plasma membrane of endothelium
155. Immunoisolation of caveolae with high affinity antibody binding to the oligomeric caveolin cage. Toward understanding the basis of purification
156. EHSH1/intersectin, a protein that contains EH and SH3 domains and binds to dynamin and SNAP-25. A protein connection between exocytosis and endocytosis?
157. A network approach to the systematic analysis of yeast gene function
158. Filipin-dependent inhibition of cholera toxin: Evidence for toxin internalization and activation through caveolae-like domains
159. A structural explanation for the binding of multiple ligands by the alpha-adaptin appendage domain
160. Crystal structure of the amphiphysin-2 SH3 domain and its role in the prevention of dynamin ring formation
161. Regulated internalization of caveolae
162. A functional PtdIns(3)P-binding motif
163. Combinatorial control in ubiquitin-dependent proteolysis: Don't Skp the F-box hypothesis
164. Protein transport to the vacuole and receptor-mediated endocytosis by clathrin heavy chain-deficient yeast
165. A test of clathrin function in protein secretion and cell growth
166. Vac1p coordinates Rab and phosphatidylinositol 3-kinase signaling in Vps45p-dependent vesicle docking/fusion at the endosome
167. Transport-vesicle targeting: Tethers before SNAREs
168. Mechanism of Nef-induced CD4 endocytosis: Nef connects CD4 with the mu chain of adaptor complexes
169. VPS27 controls vacuolar and endocytic traffic through a prevacuolar compartment in Saccharomyces cerevisiae
170. Syndapin isoforms participate in receptor-mediated endocytosis and actin organization
171. Syndapin I, a synaptic dynamin-binding protein that associates with the neural Wiskott-Aldrich syndrome protein
172. Regulatory interactions in the recognition of endocytic sorting signals by AP-2 complexes
173. end3 and end4: Two mutants defective in receptor-mediated and fluid-phase endocytosis in Saccharomyces cerevisiae
174. The carboxy-terminal segment of the yeast alpha-factor receptor is a regulatory domain
175. The differential miscibility of lipids as the basis for the formation of functional membrane rafts
176. 2+-binding domain
177. Extraction of cholesterol with methyl-beta-cyclodextrin perturbs formation of clathrin-coated endocytic vesicles
178. Extraction of cholesterol with methyl-beta-cyclodextrin perturbs formation of clathrin-coated endocytic vesicles
179. Identification of a novel sequence mediating regulated endocytosis of the G protein-coupled alpha-pheromone receptor in yeast
180. The epsins define a family of proteins that interact with components of the clathrin coat and contain a new protein module
181. A large PEST-like sequence directs the ubiquitination, endocytosis, and vacuolar degradation of the yeast a-factor receptor
182. Caveolin, a protein component of caveolae membrane coats
183. Cholesterol controls the clustering of the glycophospholipid-anchored membrane receptor for 5-methyltetrahydrofolate
184. Phosphatidylinositol 45-biphosphate induces actin-based movement of raft-enriched vesicles through WASP-Arp2/3
185. Selective membrane recruitment of EEA1 suggests a role in directional transport of clathrin-coated vesicles to early endosomes
186. Binding specificity and in vivo targets of the EH domain, a novel protein-protein interaction module
187. Distinct specificity in the recognition of phosphoinositides by the pleckstrin homology domains of dynamin and Bruton's tyrosine kinase
188. Saccharomyces cerevisiae arc35p works through two genetically separable calmodulin functions to regulate the actin and tubulin cytoskeletons
189. Clathrin-coated vesicle formation and protein sorting: An integrated process
190. Dynamin and its partners: A progress report
191. Endophilin I mediates synaptic vesicle formation by transfer of arachidonate to lysophosphatidic acid
192. Filipin-sensitive caveolae-mediated transport in endothelium: Reduced transcytosis, scavenger endocytosis, and capillary permeability of select macromolecules
193. Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting
194. The EH and SH3 domain Ese proteins regulate endocytosis by linking to dynamin and Eps15
195. A yeast t-SNARE involved in endocytosis
196. Impairment of dynamin's GAP domain stimulates receptor-mediated endocytosis
197. The receptor recycling pathway contains two distinct populations of early endosomes with different sorting functions
198. Monoubiquitin carries a novel internalization signal that is appended to activated receptors
199. Distinct roles for the p110alpha and hVPS34 phosphatidylinositol 3′-kinases in vesicular trafficking, regulation of the actin cytoskeleton, and mitogenesis
200. Yeast clathrin has a distinctive light chain that is important for cell growth
201. Functional rafts in cell membranes
202. EEA1 links PI(3)K function to Rab5 regulation of endosome fusion
203. SH3-domain- containing proteins function at distinct steps in clathrin-coated vesicle formation
204. Use of a synthetic lethal screen to identify yeast mutants impaired in endocytosis, vacuolar protein sorting and the organization of the cytoskeleton
205. Synaptojanin family members are implicated in endocytic membrane traffic in yeast
206. Role of three rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the endocytic and vacuolar protein sorting pathways of yeast
207. Yeast Ypt51p and mammalian Rab5: Counterparts with similar function in the early endocytic pathway
208. Role of phosphorylation in regulation of the assembly of endocytic coat complexes
209. Caveolins, liquid-ordered domains, and signal transduction
210. Clustered folate receptors deliver 5-methyltetrahydrofolate to cytoplasm of MA104 cells
211. Epidermal growth factor receptor interaction with clathrin adaptors is mediated by the Tyr974-containing internalization motif
212. The yeast Npi1/Rsp5 ubiquitin ligase lacking its N-terminal C2 domain is competent for ubiquitination but not for subsequent endocytosis of the gap1 permease
213. 4+-induced down-regulation of the Saccharomyces cerevisiae Gap1p permease involves its ubiquitination with lysine-63-linked chains
214. Disruption of three phosphatidylinositol-polyphosphate 5-phosphatase genes from Saccharomyces cerevisiae results in pleiotropic abnormalities of vacuole morphology, cell shape, and osmohomeostasis
215. Endosomal localization of the autoantigen EEA1 is mediated by a zinc-binding FYVE finger
216. Identification and characterization of an essential family of inositol polyphosphate 5-phosphatases (INP51, INP52 and INP53 gene products) in the yeast Saccharomyces cerevisiae
217. Nucleotide-dependent conformational changes in dynamin: Evidence for a mechanochemical molecular spring
218. The ubiquitin-proteasome system and endocytosis
219. The ubiquitin conjugation system is required for ligand-induced endocytosis and degradation of the growth hormone receptor
220. Acute cholesterol depletion inhibits clathrin-coated pit budding
221. Progress in protrusion: The tell-tale scar
222. Dynamin undergoes a GTP-dependent conformational change causing vesiculation
223. Generation of coated intermediates of clathrin-mediated endocytosis on protein-free liposomes
224. Functional partnership between amphiphysin and dynamin in clathrin-mediated endocytosis
225. The phosphatidylinositol 3-phosphate binding protein Vac1p interacts with a Rab GTPase and a Sec1p homologue to facilitate vesicle-mediated vacuolar protein sorting
226. Clathrin facilitates the internalization of seven transmembrane segment receptors for mating pheromones in yeast
227. The sequence NPFXD defines a new class of endocytosis signal in Saccharomyces cerevisiae
228. The EH-domain-containing protein Pan1 is required for normal organization of the actin cytoskeleton in Saccharomyces cerevisiae
229. EH domain proteins Pan1p and End3p are components of a complex that plays a dual role in organization of the cortical actin cytoskeleton and endocytosis in Saccharomyces cerevisiae
230. Pan1p, End3p, and S1a1p, three yeast proteins required for normal cortical actin cytoskeleton organization, associate with each other and play essential roles in cell wall morphogenesis
231. Eps15 is a component of clathrin-coated pits and vesicles and is located at the rim of coated pits
232. A function for monoubiquitination in the internalization of a G protein-coupled receptor
233. Cholesterol binds to synaptophysin and is required for biogenesis of synaptic vesicles
234. Signalling through the lipid products of phosphoinositide-3-OH kinase
235. Eps15 is recruited to the plasma membrane upon epidermal growth factor receptor activation and localizes to components of the endocytic pathway during receptor internalization
236. Signal-dependent membrane protein trafficking in the endocytic pathway
237. An acidic cluster in the cytosolic domain of human cytomegalovirus glycoprotein B is a signal for endocytosis from the plasma membrane
238. Membrane association of Rab5 mediated by GDP-dissociation inhibitor and accompanied by GDP/GTP exchange
239. Actin-binding verprolin is a polarity development protein required for the morphogenesis and function of the yeast actin cytoskeleton
240. Importance of the pleckstrin homology domain of dynamin in clathrin-mediated endocytosis
241. Epidermal growth factor induces ubiquitination of Eps15
242. Dynamin-like protein encoded by the Drosophila shibire gene associated with vesicular traffic
243. Endocytosis and degradation of the growth hormone receptor are proteasome-dependent
244. Induction of caveolae in the apical plasma membrane of Madin-Darby canine kidney cells
245. An acidic sequence within the cytoplasmic domain of furin functions as a determinant of trans-Golgi network localization and internalization from the cell surface
246. CtBP/BARS induces fission of Golgi membranes by acylating lysophosphatidic acid
247. Molecular characterization of VAC1, a gene required for vacuole inheritance and vacuole protein sorting
248. Pan1p, yeast eps15, functions as a multivalent adaptor that coordinates protein-protein interactions essential for endocytosis
249. Protein traffic in the yeast endocytic and vacuolar protein sorting pathways
250. A novel fluorescence-activated cell sorter-based screen for yeast endocytosis mutants identifies a yeast homologue of mammalian eps15
251. Yeast epsins contain an essential N-terminal ENTH domain, bind clathrin and are required for endocytosis
252. End4p/Sla2p interacts with actin-associated proteins for endocytosis in Saccharomyces cerevisiae
253. The role of ADP-ribosylation factor and phospholipase D in adaptor recruitment
254. The F-box protein Rcy1p is involved in endocytic membrane traffic and recycling out of an early endosome in S. cerevisiae
255. Amphiphysin heterodimers: Potential role in clathrin-mediated endocytosis
256. Inhibition of receptor-mediated endocytosis by the amphiphysin SH3 domain
257. In mouse brain profilin I and profilin II associate with regulators of the endocytic pathway and actin assembly
258. Inositol lipid 5-phosphatases - Traffic signals and signal traffic
259. Phosphoinositide signaling and turnover: PtdIns(3)P, a regulator of membrane traffic, is transported to the vacuole and degraded by a process that requires lumenal vacuolar hydrolase activities
260. Phosphoinositide 3-kinases and their FYVE domain-containing effectors as regulators of vacuolar/lysosomal membrane trafficking pathways
261. Intersectin, a novel adaptor protein with two Eps15 homology and five Src homology 3 domains
262. A role for the actin cytoskeleton of Saccharomyces cerevisiae in bipolar budsite selection
263. Endocytosis: Is dynamin a 'blue collar' or 'white collar' worker?
264. Adaptor complex-independent clathrin function in yeast
265. Sphingoid base synthesis requirement for endocytosis in Saccharomyces cerevisiae
266. Two separate functions are encoded by the carboxyl-terminal domains of the yeast cyclase-associated protein and its mammalian homologs. Dimerization and actin binding
267. Regulation of the actin cytoskeleton organization in yeast by a novel serine/threonine kinase Prk1p
268. Sphingomyelinase treatment induces ATP-independent endocytosis
269. Synaptotagmin I is a high affinity receptor for clathrin AP-2: Implications for membrane recycling
270. Identification of the binding site for acidic phospholipids on the pH domain of dynamin: Implications for stimulation of GTPase activity
271. MDP1, a Saccharomyces cerevisiae gene involved in mitochondrial/cytoplasmic protein distribution, is identical to the ubiquitin-protein ligase gene RSP5