EEA1 links PI(3)K function to Rab5 regulation of endosome fusion

Nature

Volumn 394, Issue 6692, 1998, Pages 494-498

  Simonsen, Anne ^a   Lippé, Roger ^b,e   Christoforidis, Savvas ^b,e   Gaullier, Jean Michel ^a   Brech, Andreas ^c   Callaghan, Judy ^d   Toh, Ban Hock ^d   Murphy, Carol ^b,e,f   Zerial, Marino ^b,e   Stenmark, Harald ^a  

^a OSLO UNIVERSITY HOSPITAL   (Norway)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^c UNIVERSITY OF OSLO   (Norway)

^d MONASH UNIVERSITY MEDICAL SCHOOL   (Australia)

^e MAX PLANCK INSTITUTE OF MOLECULAR CELL BIOLOGY AND GENETICS   (Germany)

^f UNIVERSITY OF IOANNINA   (Greece)

Author keywords

[No Author keywords available]

Indexed keywords

AUTOANTIGEN; EARLY ENDOSOME ANTIGEN 1; GUANOSINE TRIPHOSPHATASE; PHOSPHATIDYLINOSITOL 3 KINASE; UNCLASSIFIED DRUG;

AMINO ACID COMPOSITION; ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CELLULAR DISTRIBUTION; ENDOCYTOSIS; ENDOSOME; ENZYME ACTIVITY; GENE ACTIVATION; HELA CELL; MEMBRANE FUSION; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION;

1-PHOSPHATIDYLINOSITOL 3-KINASE; ANDROSTADIENES; ANIMALS; AUTOANTIGENS; CATTLE; CELL LINE; CLONING, MOLECULAR; CRICETINAE; ENDOSOMES; ENZYME INHIBITORS; GTP-BINDING PROTEINS; GUANOSINE TRIPHOSPHATE; HELA CELLS; HUMANS; INTRACELLULAR MEMBRANES; MEMBRANE FUSION; MEMBRANE PROTEINS; MUTAGENESIS; RAB5 GTP-BINDING PROTEINS; RECOMBINANT FUSION PROTEINS; VESICULAR TRANSPORT PROTEINS;

EID: 0032581654     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/28879     Document Type: Article

References (26)

1. Novick, P. & Zerial, M. The diversity of Rab proteins in vesicle transport. Curr. Opin. Cell Biol. 9, 496-504 (1997).
2. Olkkonen, V. M. & Stenmark, H. Role of rab GTPases in membrane traffic. Int. Rev. Cytol. 176, 1-85 (1997).
3. Roth, M. G. & Sternweis, P. C. The role of lipid signaling in constitutive membrane traffic. Curr. Opin. Cell Biol. 9, 519-526 (1997).
4. De Camilli, P., Emr, S. D., McPherson, P. S. & Novick, P. Phosphoinositides as regulators in membrane traffic. Science 271, 1533-1539 (1996).
5. Li, G. et al. Evidence for phosphatidylinositol 3-kinase as a regulator of endocytosis via activation of Rab5. Proc. Natl Acad. Sci. USA 92, 10207-10211 (1995).
6. Jones, A. T. & Clague, M. J. Phosphatidylinositol 3-kinase activity is required for early endosome fusion. Biochem. J. 311, 31-34 (1995).
7. Spiro, D. J., Boll, W., Kirchhausen, T. & Wessling-Resnick, M. Wortmannin alters the transferrin receptor endocytic pathway in vivo and in vitro. Mol Biol. Cell 7, 355-367 (1996).
8. Gorvel, J. P., Chavrier, P., Zerial, M. & Gruenberg, J. rab5 controls early endosome fusion in vitro. Cell 64, 915-925 (1991).
9. Jones, A. T., Mills, I. G., Scheidig, A. J., Alexandrov, K. & Clague, M. J. Inhibition of endosome fusion by wortmannin persists in the presence of activated rab5. Mol. Biol. Cell 9, 323-332 (1998).
10. Mu, F. T. et al. EEA1, an early endosome-associated protein. EEA1 is a conserved alpha-helical peripheral membrane protein flanked by cysteine "fingers" and contains a calmodulin-binding IQ motif. J. Biol. Chem. 270, 13503-13511 (1995).
11. Patki, V., Virbasius, J., Lane, W. S., Toh, B. H., Shpetner, H. S. & Corvera, S. Identification of an early endosomal protein regulated by phosphatidylinositol 3-kinase. Proc. Natl Acad. Sci. USA 94, 7326-7330 (1997).
12. Gaullier, J.-M. et al. A functional PtdIns(3)P-binding motif. Nature 394, 433-434 (1998).
13. Stenmark, H., Aasland, R., Toh, B. H. & D'Arrigo, A. Endosomal localization of the autoantigen EEA1 is mediated by a zinc-binding FYVE finger. J. Biol. Chem. 271, 24048-24054 (1996).
14. Vitale, G. et al. Distinct Rab-binding domains mediate the interaction of rabaptin-5 with GTP-bound rab4 and rab5. EMBO J. 17, 1941-1951 (1998).
15. Stenmark, H., Parton, R. G., Steele-Mortimer, O., Lütcke, A., Gruenberg, J. & Zerial, M. Inhibition of rab5 GTPase activity stimulates membrane fusion in endocytosis. EMBO J. 13, 1287-1296 (1994).
16. Horiuchi, H. et al. A novel Rab5 GDP/GTP exchange factor complexed to rabaptin-5 links nucleotide exchange to effector recruitment and function. Cell 90, 1149-1159 (1997).
17. Shpetner, H., Joly, M., Hartley, D. & Corvera, S. Potential sites of PI-3 kinase function in the endocytic pathway revealed by the PI-3 kinase inhibitor, wortmannin. J. Cell Biol. 132, 595-605 (1996).
18. Rybin, V. et al. GTPase activity of Rab5 acts as a timer for endocytic membrane fusion. Nature 383, 266-269 (1996).
19. D'Arrigo, A., Bucci, C., Toh, B. H. & Stenmark, H. Microtubules are involved in bafilomycin A1-induced tubulation and Rab5-dependent vacuolation of early endosomes. Eur. J. Cell Biol. 72, 95-103 (1997).
20. Stenmark, H., Vitale, G., Ullrich, O. & Zerial, M. Rabaptin-5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion. Cell 83, 423-432 (1995).
21. Chavrier, P., Parton, R. G., Hauri, H. P., Simons, K. & Zerial, M. Localization of low molecular weight GTP binding proteins to exocytic and endocytic compartments. Cell 62, 317-329 (1990).
22. Bucci, C. et al. The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway. Cell 70, 715-728 (1992).
23. Vojtek, A. B., Hollenberg, S. M. & Cooper, J. A. Mammalian Ras interacts directly with the serine/ threonine kinase Raf. Cell 74, 205-214 (1993).
24. Stenmark, H., Bucci, C. & Zerial, M. Expression of Rab GTPases using recombinant vaccinia viruses. Methods Enzymol. 257, 155-164 (1995).
25. Evan, G. I., Lewis, G. K., Ramsay, G. & Bishop, J. M. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell Biol. 5, 3610-3616 (1985).
26. Tooze, J. & Hollinshead, M. Tubular early endosome networks in AtT20 and other cells. J. Cell Biol. 115, 635-653 (1991).