Catabolite inactivation of the yeast maltose transporter requires ubiquitin-ligase npi1/rsp5 and ubiquitin-hydrolase npi2/doa4

FEMS Microbiology Letters

Volumn 147, Issue 2, 1997, Pages 273-277

  Lucero, Pilar ^a   Lagunas, Rosario ^a  

^a INSTITUTO DE INVESTIGACIONES BIOMÉDICAS ALBERTO SOLS   (Spain)

Author keywords

catabolite inactivation; endocytosis; maltose transporter; proteolysis; Saccharomyces cerevisiae; ubiquitin pathway

Indexed keywords

CARRIER PROTEIN; MALTOSE BINDING PROTEIN;

ARTICLE; ENDOCYTOSIS; MUTANT; PRIORITY JOURNAL; PROTEIN DEGRADATION; REACTION ANALYSIS; SACCHAROMYCES CEREVISIAE;

CARRIER PROTEINS; CHEMILUMINESCENT MEASUREMENTS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOCYTOSIS; ENDOPEPTIDASES; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, FUNGAL; IMMUNOBLOTTING; LIGASES; MALTOSE; PLASMIDS; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSFORMATION, GENETIC; UBIQUITIN-PROTEIN LIGASE COMPLEXES; UBIQUITIN-PROTEIN LIGASES;

EID: 0031055949     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(96)00538-1     Document Type: Article

References (14)

1. Busturia, A. and Lagunas, R. (1985) Identification of two forms of the maltose transport system in Saccharomyces cerevisiae and their regulation by catabolite inactivation. Biochim. Biophys. Acta 820, 324-326.
2. Lucero, P., Herweijer, M. and Lagunas R. (1993) Catabolite inactivation of the yeast maltose transporter is due to proteolysis. FEBS Lett. 333, 165-169.
3. Riballo, E., Herweijer, M., Wolf, D.H. and Lagunas, R. (1995) Catabolite inactivation of the yeast maltose transporter occurs in the vacuole after internalization by endocytosis. J. Bacteriol. 177, 5622-5627.
4. Hochstrasser, M. (1995) Ubiquitin, proteasomes, and regulation of intracellular protein degradation. Curr. Opin. Cell Biol. 7, 215-223.
5. Kolling, R. and Hollenberg, C. (1994) The ABC-transporter Ste6 accumulates in the plasma membrane in a ubiquitinated form in endocytosis mutants. EMBO J. 13, 3261-3271.
6. Hein, C., Springael, J.Y., Volland, C., Haguenauer-Tsapis, R. and André, B. (1995) NPI1, an essential yeast gene involved in induced degradation of Gap1 and Fur4 permeases, encodes the Rsp5 ubiquitin-protein ligase. Mol. Microbiol. 18, 77-87.
7. Egner, E. and Kuchler, K. (1996) The yeast multidrug transporter Pdr5 of the plasma membrane is ubiquitinated prior to endocytosis and degradation in the vacuole. FEBS Lett. 378, 177-181.
8. Galan, J.M., Moreau, V., André, B., Volland C. and Haguenauer-Tsapis, R. (1996) Ubiquitination mediated by the Npi1p/Rsp5p ubiquitin-protein ligase is required for endocytosis of the yeast uracil permease. J. Biol. Chem. 271, 10946-10952.
9. Hicke, L. and Riezman, H. (1996) Ubiquitination of a yeast plasma membrane receptor signals its ligand-stimulated endocytosis. Cell 84, 277-287.
10. Papa, F.R. and Hochstrasser, M. (1993) The yeast DOA4 gene encodes a deubiquitinating enzyme related to a product of the human tre-2 oncogene. Nature 366, 313-319.
11. Rodicio, R. (1986) Insertion of non-homologous DNA sequences into a regulatory gene cause a constitutive maltase synthesis in yeast. Curr. Genet. 11, 235-241.
12. Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 267-275.
13. Bartel, B., Wunning, I and Varshavsky, A (1990) The recognition component of the N-end rule pathway. EMBO J. 9, 3179-3189.
14. Springael, J.-Y., Decraene, J.O., Hein, C. and André, B. (1996) Both ubiquitin-ligase Npi1/Rsp5 and ubiquitin hydrolase Npi2/Doa4 are required for ammonium-induced inactivation and degradation of the general amino-acid permease (Gapl) of Saccharomyces cerevisiae. Proc. 14th Small Meeting on Yeast Transport and Energetics, p. 25.